HomeMy WebLinkAboutHMP 09-05; LAKE CALAVERA TRAILS MASTER PLAN; LIMITED ENVIRONMENTAL ANALYSIS AGUA HEDIONDA CREEK; 2006-02-10I Agua Hedionda Creek February 10, 2006 -
I Carlsbad, California Project No. 105760001
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I APPENDIXA
NOAA SQniRT TABLES
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I 105760001 R.doc
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Table 2. Applicable Sediment Quality Objectives.
Analyte I Sediment Quality Objective
Total Aluminum N/A
Total Cadmium 3.53 mg/kg
Total Chromium(III) 90.0 mg/kg
Total Copper 197.0 mg/kg
Total Lead 91.3 mg/kg
Total Nickel 35.9 mg/kg
Total Zinc 315 mg/kg
Total PAHs N/A
Oil and Grease N/A
Acute Toxicity N/A
NIA-no quality objectives for sediments are available.
Source: NOAA SQUIRT Levels for lnorganics in Solids
-------------------
This set of NOAA Screening Quick Reference Tables, or
SQuiRTs, presents screening concentrations for inorganic and
organic contaminants in various environmental media. Guidelines for
sample preservation and options for laboratory analytical techniques
are also included.
The SQuiRT cards were developed for internal use by the Coastal
Protection & Restoration Division (CPR) of NOAA. The CPR Division
identifies potential impacts to coastal resources and habitats likely
to be affected by hazardous waste sites. To initially Identify
substances which may threaten resources of concern to NOAA,
environmental concentrations are compared to these screening levels.
These tables are intended for preliminary screening purposes only:
they do not represent official NOAA policy and do not constitute
criteria or clean-up levels. NOAA does not endorse their use for any
other purposes. Screening levels are reported with the number of
significant figures they were originally reported with.
Further guidance on the recommended application of various
screening guidelines is provided in the supporting source
documentation (listed on the last page of each section). Users of
the SQuiRT cards are strongly encouraged to review supporting
documentation to determine appropriateness for their specific use.
The SQuiRT card set is organized into the following sections:
• lnorganics in Solids (freshwater and marine sediment, plus soil)
• lnorganics in Water (groundwater and surface water)
• Organics in Water and Solids
• Analytical Methods for lnorganics
• Analytical Methods for Organics
• Guidelines for Sample Collection & Storage
For surface water samples, the CPR Division compares measured
contaminant concentrations to their applicable, EPA Ambient Water
Quality Criteria (AWQC) for the protection of aquatic organisms.
Because releases from hazardous waste sites are often continuous
and long-term, concentrations are compared directly with the chronic
AWQC, when available. SQuiRTs for trace element AWQCs have
been updated to show values for just filtered samples, as well as the
formulae to calculate exact criteria for elements whose criteria are
hardness-dependent. Groundwater concentrations are also screened
against AWQC. However, given the dilution expected during migration
and upon discharge of groundwater to surface water, CPRD uses 10
times the applicable AWQC for screening. If available, suitable site-
specific dilution factors are used. Maximum Contaminant Levels
(MCLs), applicable to drinking water sources and secondary MCLs
applicable to groundwater, are also provided on the SQuiRT cards.
Promulgated criteria similar to the AWQC are generally not avallable
for contaminated soils or sediments. For screening purposes,
inorganic contaminant levels in soils are compared to the average
concentrations found in natural soils of the United States. Organic
compounds in soil are screened against risk-based Canadian soil
standards. Soil standards for different land use categories are listed
to provide perspective. Soil values are not used by NOAA to estimate
aquatic exposures. NOAA screens soil concentrations only to
estimate which contaminants may be elevated and thus represent
potential contaminant sources to aquatic habitats of concern.
Multiple sediment screening values have been included in the NOAA
SQuiRTs to help portray the entire spectrum of concentrations
which have been associated with various probabilities of adverse
biological effects. This spectrum ranges from presumably non-toxic
e.g., trace metal levels reported to represent non-anthropogenically
CONflNU!:D ON LA sf PAGE 1
UPDATED 5 EPTEM BER, 1999 HAZMAT REPORT99-1
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Agua Hedionda Creek
Carlsbad, California
APPENDIXB
February 10, 2006
Project No. 105760001
GRADATION TEST RESULTS AND SAND EQUIVALENT VALUES
105760001 R doc
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I GRAVEL I SAND FINES
I Coarse I Fine I Coarse! Medium Fine SILT I CLAY
U.S. STANDARD SIEVE NUMBERS HYDROMETER
3" 2" 1½" ..:!." ¾". ½" ¾" 4 10 16 30 50 100 200
100.0 j\ I I ~I I I I I
90.0 I I I I
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(9 I I \1 I
ui I I I I I I I I I ~ 60.0 ' >-I I I I
m I I I I I I I I I I c:: 50.0 w I I r I z I I I I I I I I I u:
I-40.0 I I I I z w I I I I I I I I I I 0 c:: 30.0 I I I I I w a. i I I I I I I I I I 20.0 I I I I I I I I \ I I
10.0 I I I I I I I I I\ I I
I I I I I I I I \ '--I I
I I. I ~ .L 0.0
100.0000 10.0000 1.0000 0.1000 0.0100 0.0010
GRAIN SIZE IN MILLIMETERS
Sample Depth Liquid Plastic Plasticity Dao Cu Cc
Passing
Symbol Location (ft) Limit Limit Index D10 D30 No.200
(%)
• CCAH-01 0.0-2.0 ---0.40 0.78 1.40 3.5 1.1 0
PERFORMED IN GENERAL ACCORDANCE WITH ASTM D 422-63 (02)
l(in90&1(\oo-re GRADATION TEST RESULTS
PROJECT NO. DATE Agua Hedionda-City of Carlsbad
105760001 1/06 Carlsbad, California
105760001 SIEVE NM-CCAH-01 @ 0.0.2.0 xis
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0.0001
u.s.c.s
SP
FIGURE
8-1
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I GRAVEL I SAND FINES
I Coarse I Fine I Coarse Medium Fine SILT I CLAY
U.S. STANDARD SIEVE NUMBERS HYDROMETER
3" 2" 1½" 1"¾" ½""a" 4 10 16 30 50 100 200 100.0 I I I I I I or,. I I I I I"\.
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100.0000 10.0000 1.0000 0.1000 0.0100 0.0010
GRAIN SIZE IN MILLIMETERS
Sample Depth Liquid Plastic Plasticity Passing
Symbol Location (ft) Limit Limit Index 010 030 D50 Cu Cc No. 200
{%)
• CCAH-02 0.0-2.0 ---0.14 0.58 1.10 7.9 2.2 5
PERFORMED IN GENERAL ACCORDANCE WITH ASTM D 422-63 (02)
}(in9o&iV\Oo~e GRADATION TEST RESULTS
PROJECT NO. DATE Agua Hedionda-City of Carlsbad
105760001 1/06 Carlsbad, California
105760001 SIEVE NM-CCAH-02@ 0.0-2 0.xls
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0.0001
u.s.c.s
SW-SM
FIGURE
8-2
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Agua Hedionda Creek
Carlsbad, California
APPENDIXC
February 10, 2006
Project No. 105760001
ANALYTICAL REPORTS AND CHAIN-OF-CUSTODY DOCUMENTATION
105760001 R.doc
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Agua Hedionda Creek
Carlsbad, California
APPENDIXB
February 10, 2006
Project No. 105760001
GRADATION TEST RESULTS AND SAND EQUIVALENT VALUES
105760001 R.doc
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I GRAVEL I SAND FINES
I Coarse I Fine . I Coarse I Medium Fine SILT I CLAY
U.S. STANDARD SIEVE NUMBERS HYDROMETER
3" 2" 1½" .!." ¾" ½" ¾" 4 10 16 30 50 100 200 100.0
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90.0 I I I
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>-I I I I
ro I I I I I I I I I I a::: 50.0 w I I 0 I z I I I I I I I I I u:
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10.0 I I I I I I I I \ I I
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100.0000 10.0000 1.0000 0.1000 0.0100 0.0010
GRAIN SIZE IN MILLIMETERS •
'
Sample Depth Liquid Plastic Plasticity Passing
Symbol Location (ft) Limit Limit Index D10 D30 Deo Cu Cc No.200
(%)
• CCAH-01 0.0-2.0 ---0.40 0.78 1.40 3.5 1.1 0
PERFORMED IN GENERAL ACCORDANCE WITH ASTM D 422-63 (02)
JYin90&.JV\Oore GRADATION TEST RESULTS
PROJECT NO. DATE Agua Hedionda-City of Carlsbad
105760001 1/06 Carlsbad, California
105760001 SIEVE NM-CCAH-01, @ 0 0-2.0.xls
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0.0001
u.s.c.s
SP
FIGURE
8-1
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Agua Hedionda Creek
Carlsbad, California
APPENDIXC
February 10, 2006
Project No. 105760001
ANALYTICAL REPORTS AND CHAIN-OF-CUSTODY DOCUMENTATION
105760001 R doc
--------CALSCIENCE ENVIRONMENTAL
LABORATORIES, INC.
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TEL: (714) 895-5494 • FAX: (714) 894-7501
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i'\U:<NARwUND TIME: \. ~ V \,, y ~ □SAME DAY 024HR 048HR □12HR )i'soAYs 0 10DAYS REQUESTED ANALYSES ... SPECIAL REQUIREMENTS (ADDITIONAL COSTS MAY APPLY) ,
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Please note that oaAes 1 and 2 of 2 of our T/Cs are printed on the reverse side of the Green and Yellow copies respectively. 10120/04 Revision
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APPENDIX A
SUMMARY OF SURVIVAL AND GROWTH RESULTS
Page23 of50
Appendix A-1. Summary of.Results for 10-day Amphipod Survival and Growth
Calscience Environmental Laboratories
Test Initiation Date: January 4, 2006
Am phi pod -Hya/el/a azteca
Mean Total Ash-Free Ash-Free Dry Mean Ash-Free Dry
' Site Rep # Alive % Survival % Survival Dry Weight (mg) Weight per Org. (mg) Weight per Org. (mg)
Control A 16 80 0.220 0.014
B 12 60 0.130 0.011
C 20 100 0.460 0.023
D 13 65 0.190 0.015
E 20 100 0.610 0.031
Surr 11 85 82 0.520 0.031 0.021
Site NM-CCAH-01 A 15 75 0.370 0.025
B 18 90 0.350 0.019
C 19 95 0.400 0.021
D 19 95 0.610 0.032
E 17 85 0.390 0.023
Surr 20 ·100 90 0.500 0.025 0.024
Site NM-CCAH-02 A 19 95 0.540 0.028
B 15 75 0.100 0.007
C 17 85 0.440 0.026
D 15 75 0.180 0.012
E 11 55 0.120 0.011 ~ Surr 13 65 75 0.330 0.025 0.018 ~ ~ (I)
N ~
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- - - -
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I Page 25 of 50
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I APPENDIX 8
I WATER QUALITY RESULTS
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I Page 29 of SO
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I APPENDIXC
I REFERENCE TOXICANT TESTS
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Page30 of50 I
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• REFERENCE TOXICANT TEST 1 I
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I CETIS Test Summary
I Acute Amphlpod Survival Test
Test No: 18-1465-1616
Start Date: 04 Jan-06 02:00 PM
Ending Date: 08 Jan-06 02:00 PM
Setup Date: 04 Jan-06 02:00 PM
Sample No: 18-9355-9446
Sample Date: 04 Jan-06
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Receive Date: 04 Jan-06
Sample Age: 14h
Comparison Summary
Analysis Endpoint
12-04 78-9376 96h Proportion Survived
I Point Estimate Summary
Analysis Endpoint
14-2282-9276 96h Proportion Survived
I 96h Proportion Survived Summary
Conc-µg/L Control Type Reps
0 Lab Control 4
100 4
200 4
400 4
800 4
1600 4
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96h Proportion Survived Detail
Conc-µg/L
0
100
200
400
800
1600 I
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10-089-125-2
I
Control Type Rep 1
Lab Control 1.00000
0.80000
0.40000
0.20000
0.00000
0.00000
Test Type: Survival (96h)
Protocol: EPA/600/R-99/064 (2000)
Oil Water: Culligan Filtered Water
Brine: Not Applicable
Material: Copper chloride
Code: 060104HARA
Source: Reference Toxicant
Station:
NOEL LOEL ChV
100 200 141.421
% Effect Conc-µg/L 95% LCL
25 202.61630 128.93070
50 283.50090 213.47630
Mean Minimum Maximum SE
0.85000 0.70000 1.00000 0.06455
0.80000 0.70000 0.90000 0.04082
0.60000 0.40000 0.70000 0.07071
0.25000 0.00000 0.50000 0.10408
0.00000 0.00000 0.00000 0.00000
0.00000 0.00000 0.00000 0.00000
Rep 2 Rep 3 Rep4
0.70000 0.90000 0.80000
0.70000 0.80000 0.90000
0.70000 0.60000 0.70000
0.50000 0.30000 0.00000
0.00000 0.00000 0.00000
0.00000 0.00000 0.00000
CETIS™ v1.025B
Duration:
Species:
Source:
Client:
Project:
MSDp
23.77%
95% UCL
256.23410
342.83280
SD
0.12910
0.08165
0.14142
0.20817
0.00000
0.00000
Page 31 of Silage 1 of 1
Report Date: 10 Jan-06 12:08 PM
Link: 17-4075-6960/060104HARA
Nautilus Environmental (CA)
96h
Hyalella azteca
Aquatic Biosystems, CO
Internal
Method
Dunnett's Multiple Comparison
Method
Linear Regression
CV
15.19%
10.21%
23.57%
83.27%
0.00%
0.00%
Analyst:fu Approval:~ \ -2 ~-()5
C~mparlsonsrage 32 of 5.,,age 1 of 1
CETIS Analysis Detail Report Date: 10 Jan-06 12:08 PM
Analysis: 12-0478-9376/060104HARA
Acute Amphipod Survival Test Nautilus Environmental (CA)
Endpoint Analysis Type Sample Link Control Link Date Analyzed Version
96h Proportion Survived Comparison 17-4075-6960 17-4075-6960 10 Jan-06 12:06 PM CETISv1.025
l'y1ethod Alt H Data Transform z NOEL LOEL Toxic Units ChV MSDp
Dunnett's Multiple Comparison C>T Angular (Corrected) 100 200 1.00 141.421 23.77%
ANOVA Assumptions
Attribute Test Statistic Critical P Level Declslon(0.01)
Variances Modified Levene 2.96073 4.24788 .0.04020 Equal Variances .
Distribution Shapiro-Wilk W 0.91996 0.88421 0.06148 Normal Distribution
ANOVA Table
Source Sum of Squares Mean Square DF F Statistic P Level Declslon(0.05)
Between 4.269273 0.8538547 5 38.35 0.00000 Significant Effect
Error. 0.4007185 0.0222621 18
Total 4.66999176 0.8761168 23
Group Comparisons
Control VS Conc-µg/L Statistic Critical P Level MSD Decfslon(0.05)
Lab Control 100 0.72241 2.41 > 0.0500 0.25426 Non-Significant Effect
200 2.85833 2.41 <= 0.0500 0.25426 Significant Effect
400 6.56825 2.41 <= 0.0500 0.25426 Significant Effect
·000 9.77274 2.41 <=0.0500 0.25426 Significant Effect
1600 9.77274 2.41 <=0.0500 0.25426 Significant Effect
Data Summary Original Data Transformed Data
Cpnc-µg/L Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum so
0 Lab Control 4 0.85000 0.70000 1.00000 0.12910 1.18984 0.99116 1.41202 0.18182
100 4 0.80000 0.70000 0.90000 0,08165 1.11362 0.99116 1.24905 0.10555
200 4 0.60000 0.40000 0.70000 0.14142 0.88828 0.68472 0.99116 0.14446
400 4 0,25000 0.00000 0.50000 0.20817 0.49687 0.15878 0,78540 0.26173
BOO 4 0.00000 0.00000 0.00000 0.00000 0.15878 0.15878 0.15878 0.00001
1600 4 0,00000 0.00000 0.00000 0.00000 0.15878 0.15878 6.15878 0.00001
Graphics
1.0
t {
0.3 0
l 0.9-: ' 0 0.2
0.8 ' ' 0 ::, Ji 0.1 0 UI 0.7 ························r····················· ..... ,~-· C 0 0 i 0.6 ss 0,0 ··----·············ooeoo' -ooo--------·······--·····
e o.s 0 1
0,. -0.1 0
.c 0.4 "' ' °' 0.3 ·0.2 0 c,
0.2
-0.3 0.1 p
o.o •0,4
0 100 200 400 800 1600 •2,0 ·1.5 •1,0 ·0.5 o.o 0.5 1.0 1.5 2.0
Conc-µg/L Ranklts
000-089-125-2 CETIS™ v1.0258 Analyst: .AC, . Approval: S IA \-2 (;, -cb
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CETIS Analysis Detail
Acute Amphipod Survival Test
Linear Regre~Rgie 33 of 5@>age 1 of 2
Report Date: 10 Jan-06 12:08 PM
Analysis: 14-2282-9276/060104 HARA
Nautilus Environmental (CA)
Endpoint Analysis Type Sample Link Control Link Date Analyzed Version
96h Proportion Survived Linear Regression 17-4075-6960 17-4075-6960 10 Jan-06 12:06 PM CETISv1 .025
Linear Regression Options
Model Threshold Option Lower Threshold Threshold Optimized Rewelghted Pooled Groups Heterogeneity Corr.
Log-Normal Control Threshold 0.15 Yes Yes No No
Regression Parameters
Parameter Estimate Std Error 95% LCL 95% UCL t Statistic P Level Declslon(0.05) -
Threshold 0.16992 0.04687 0.07807 0.26178 3.626 0.03610 Significant
Slope 4.62359 0.92991 2.80098 6.44621 4.972 0.01563 Significant
Intercept -6.33962 2.35695 -10.95924 -1.71999 -2.690 0.07443 Nol Significant
Regression Summary
lters Log Likelihood Mu Sigma G Stat Chi-Sq Critical P Level Decision(0.05)
9 -30.15384 -1.37114 0.21628 0.15539 13.31712 28.86930 0.77240 Non-Significant Heterogeneity
Residual Analysis
Attribute Method Statistic Critical P Level Decision(0.05)
Variances Modified Levene 1.29662 2.95825 0.32020 Equal Variances
Distribution Shapiro-Wilk W 0.68169 0.90456 0.00000 Non-normal Distribution
Point Estimates
% Effect Conc-µg/L 95% LCL 95% UCL
25 202.61630 128.93070 256.23410
50 283.50090 213.47630 342.83280
Data Summary Calculated Variate(A/8)
Conc-µg/L Control Type Count Mean Minimum Maximum SE SD A B
0 Lab Control 4 0.85000 0.70000 1.00000 0.02635 0.12910 34 40
100 4 0.80000 0.70000 0.90000 0.01667 0.08165 32 40
200 4 0.60000 0.40000 0.70000 0.02887 0.14142 24 40
400 4 0.25000 0.00000 0.50000 0.04249 0.20817 10 40
800 4 0.00000 0.00000 0.00000 0.00000 0.00000 0 40
1600 4 0.00000 0.00000 0.00000 0.00000 0.00000 0 40
lo0-089-125-2 CETIS™ v1.0258 Analyst: ,AC Approval: ~h \-2b-QG
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I REFERENCE TOXICANT TEST 2
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CETIS Test Summary
Acute Amphfpod Survival Test
Test No: 05-3040-8350 Test Type: Survival (96h)
Start Date: 16 Jan-06 03:00 PM Protocol: EPN600/R-99/064 (2000)
Ending Date: 20 Jan-06 01 :00 PM OIi Water: Culligan Filtered Water
Setup Date: 01 Jan-0612:00 AM Brine: Not Applicable
Sample No: 13-2548-4701 Material: Copper chloride
Sample Date: 16 Jan-06 Code: 060116HARA
Receive Date: 16 Jan-06 Source: Reference Toxlcant
Sample Age: NIA Station:
Comparison Summary
Analysis Endpoint NOEL LOEL ChV
. 10-1323-6689 96h Proportion Survived 200 400 282.843
Point Estimate Summary
Analysis Endpoint % Effect Conc-µg/L 95% LCL
13-1908-0796 96~ Proportion Survived 1 172.28180 73.61657·
5 245.79560 127.79780
10 297.06350 171.03040
15 337.56710 207.81900
20 373.66140 242.26020
25 407.68780 275.92270
40 507.81080 379.42180
50 579.53030 454.80850
96h Proportion Survived Summary
Conc-µg/L Control Type Reps Mean Minimum Maximum SE
0 Lab Control 4 0.92500 0.90000 1.00000 0.02500
100 4 0.82500 0.70000 0.90000 0.04787
200 4 0.85000 0.70000 1.00000 0.06455
400 4 0.67.500 0.60000 0.80000 0.04787
800 4 0.22500 0.10000 0.30000 0.04787
1600 4 0.02500 0.00000 0.10000 0.02500
96h Proportion Survived Detail
Conc-µg/L Contror Type Rep 1 Rep 2 Rep3 Rep4
0 Lab Control 0.90000 0.90000 0.90000 1.00000
i 100 0.70000 0.90000 0.90000 0.80000
I 200 1.00000 0.80000 0.90000 0.70000
400 0.70000 0.60000 0.60000 0.80000
800 0.30000 0.20000 0.30000 0.10000
1600 0.00000 0.00000 0.10000 0.00000
000-089-125-2 CETIS™ v1.0258
Duration:
Species:
Source:
Client:
Project:
MSDp
15.60%
95% UCL
259.67300
339.93550
393.46360
435.03490
471.89070
506.72150
61.2.00000
692.75780
so
0,05000
0.09574
0.12910
0.09574
0.09574
0.05000
Page38 of50
Page 1 of 1
Report Date: 26 Jan-06 9:56 AM
Link: 13-8083-0968/060116HARA
Nautilus Environmental (CA)
94h
Hyalella azteca
Aquatic Biosystems, CO
Internal
Method
Dunnett's Multiple Comparison
Method
Linear Regression
CV
5.41%
11.61%
15.19%
14.18%
42.55%
200.00
Analyst: .$\-\-Approval: .St\ \·'2.b-06
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CETIS Analysis Detail
Acute Amphipod Survival Test
Page 39 of 50
Comparisons: Page 1 of
Report Date:
Analysis:
26 Jan-06 9:56 AM
10-1323-6689/060116HARA
Nautilus Environmental (CA)
Endpoint Analysis Type Sample Link Control Link Date Analyzed Version
96h Proportion Survived Comparison 13-8083-0968 13-8083-0968 26 Jan-06 9:50 AM CETISv1.025
Method Alt H Data Transform z NOEL LOEL Toxic Units ChV MSDp
Dunnett's Multiple Comparison C>T Angular (Corrected) 200 400 0.50 282.843 15.60%
ANOVA Assumptions
Attribute Test Statistic Critical P Level Decisfon(0.01)
Variances Bartlett 2.57371 15.08628 0.76536 Equal Variances
Distribution Shapiro-Wilk W 0.95035 0.88421 0.28263 Normal Distribution
ANOVA Table
Source Sum of Squares Mean Square DF F Statistic P Level Decisfon(0.05)
Between 3.848681 0.7697362 5 52.47 0.00000 Significant Effect
Error 0.2640387 0.0146688 18
Total 4.11271971 0.7844050 23
Group Comparisons
Control vs Conc-µg/L Statistic Critical P Level MSD Decision(0.05)
Lab Control 100 1.64278 2.41 > 0.0500 0.2064 Non-Significant Effect
200 1.16704 2.41 > 0.0500 0.2064 Non-Significant Effect
400 3.7619 2.41 <= 0.0500 0.2064 Significant Effect
800 9.38356 2.41 <=0.0500 0.2064 Significant Effect
1600 12.7306 2.41 <= 0.0500 0.2064 Significant Effect
Data Summary Original Data Transformed Data
Conc-µg/L Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD
0 Lab Control 4 0.92500 0.90000 1.00000 0.05000 1.28979 1.24905 1.41202 0.08149
100 4 0.82500 0.70000 0.90000 0.09574 1.14910 0.99116 1.24905 0.12475
200 4 0.85000 0.70000 1.00000 0.12910 1.18984 0.99116 1.41202 0.18182
400 4 0.67500 0.60000 0.80000 0.09574 0.96761 0.88608 1.10715 0.10539
800 4 0.22500 0.10000 0.30000 0.09574 0.48617 0.32175 0.57964 0.12249
1600 4 0.02500 0.00000 0.10000 0.05000 0.19952 0.15878 0.32175 0.08149
Graphics
1.0
l l t 0.25
0
al 0.9-0.20
> 0.8 'E ............ -•• , ••••• -•• -•••• - - • -~ - - • - - - - -••• - • Reject. Nuli" -0.15 ::, ., 0
Ul 0.7 rg, C 0.10 00° 0 'f 06 ... < 3g a.as 0 8. e 0.5 u
Q. 0.00 ·------------------------------------------------------
,,: 0.4 :o
"' 0 OOCi=> en ·0.05 0.3 t 00
0.2 ·0.10
0.1 ! ·0.15 0
o.o -0.20-
0 100 200 400 800 1600 -2.0 -1.5 -1.0 ·0.5 0.0 o.s 1.0 1.5 2.0
Conc-µg/L Ranklts
000-089-125-2 CETIS™ v1.025B Analyst:-'S"'-·=\~--Approval: St\ l -2'=1-0b
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CETIS Analysis Detail
Acute Amphipod Survival Test
Page 40 of50
Linear Regression: Page 1 of 2
26 Jan-06 9:56 AM
13-1908-0796/060116HARA
Report Date:
Analysis:
Nautilus Environmental (CA)
Endpoint Analysis Type Sample Link Control Link Date Analyzed Versfop
96h Proportion Survived Linear Regression 13-8083-0968 13-8083-0968 26 Jan-06 9:52 AM • CETISv1.025
Linear Regression Options
Model Threshold Option Lower Threshold Threshold Optimized Rewelghted Pooled Groups Heterogeneity Corr.
Log-Normal Control Threshold • 0.075 Yes Yes No No
Regression Parameters
Parameter Estimate Std Error 95% LCL 95% UCL t Statistic P Level Decfslon(0.05)
Threshold 0.12612 0.03319 0.06108 0.19117 3.801 0.03199 Significant
Slope 4.41569 • 0.82534 2.79802 6.03336 5.350 0.01277 Significant
Intercept -7.20089. 2.33984 -11.78697 -2.61481 -3.078 0.05424 Not Significant
Regression Summary
fters Log Likelihood Mu Sigma G Stat Chi-Sq Critical P Level Decislon(0.05)
7 -45.21379 -1.63075 0.22647 0.13421 13.63283 28.86930 . 0.75269 Non-Significant Heterogeneity
Residual Analysis
Attribute Method Statistic Critical P Level Decislon(0.05)
Variances Modified Levene 0.40805 2.95825 0.83533 Equal Variances
Distribution Shapiro-Wilk W 0.87335 0.90456 0.01252 Non-n.ormaf Distribution
• Point Estimates
1 % Effect Conc-µg/L 95% LCL 95% UCL
11 172.28180 73.61657 259.67300
5 245.79560 127.79780 339.93550
10 297.06350 171.03040 393.46360
15 337.56710 207.81900 435.03490
20. 373.66140 242.26020 471.89070
25 407.68780 275.92270 506.72150
40 507.81080 379.42180 612.00000
50 579.53030 454.80850 692.75780
• Data Summary Calculated Varlate(A/B)
Conc-µg/L Control Type Count Mean Minimum Maximum SE SD A B
0 Lab Control 4 0.92500 0.90000 1.00000 0.01021 0.05000 37 40
100 4 0.82500 0.70000 0.90000 0.01954 0.09574 33 40
200 4 0.85000 0.70000 1.00000 0.02635 0.12910 34 40
400 4 0.67500 0.60000 0.80000 0.01954 0.09574 27 40
800 4 0.22500 0.10000 0;30000 0.01954 0.09574 9 40
1600 4 0.02500 0.00000 0.10000 0.01021 0.05000 1 40
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000-089-125-2 CETIS™ v1.025B Analyst: Sl:\: Approval: Sl-\-\-2b-~ • I
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APPENDIXD
STATISTICAL SUMMARY
Page45 of SO
Hyalella Surv Grow.pzf:Layout 1 -Fri Jan 2716:06:19 2006
Appendix Table 0-1. Unpaired t-test Summary Table
• Amphipod Survival
CalScience -Site NM-CCAH-01 and Site NM-CCAH-02
January 2006
Table Analyzed
Column A
vs
Column B
Unpaired t test
Pvalue
Parameter
P value summary
Are means signlf. different? (P < 0.05)
One-or two-tailed P value?
t, df
How big Is the difference?
Mean ± SEM of column A
Mean ± SEM of column B
Difference between means
95% cc>nfidence interval
R squared
F test to compare variances
F,DFn, Dfd
Pvalue
P value summary
Are variances significantly different?
Tab e Analyzed
• ColumnA
VS
ColumnC
Unpaired t test
Pvalue
Parameter
P value summary
Are means slgnif. different? (P < 0.05)
One-or two-tailed P value?
t, df
How big is the difference?
Mean± SEM of column A
Mean ± SEM of column C
Difference between means
95% confidence Interval
R squared
F test to compare variances
F,DFn, Dfd
Pvalue
P value summary
Are variances significantly different?
Value
Ha urvival
Control
vs
Site01
0.1557
ns
No
One-tailed
1=1.066 df=10
0.8167 ± 0.06912 N=6
0.9000 ± 0.03651 N=6
-0.08333 ± 0.07817
-0.2575 to 0.09084
0.1020
3.583, 5, 5
0.1876
/JS
No
Value
Ha urvival
Control
vs
Slte02
0.2381
·ns
No
One-tailed
t=0. 7 402 df=10
0.8167 ± 0.06912 N=6
0,7500 ± 0.05774 N=6
0,06667 ± 0.09006
-o. 1340 to 0.2673
0.05195
1.433, 5, 5
0.7024
ns
No
I Page46 of50
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