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Home My WebLink About; Carlsbad Golf Course Water Quaility Study; Carlsbad Golf Course Water Quaility Study; 2007-04-04I •3 * cxmMVOK-w.en.-Ul Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Testing Period: February 2007 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 April 4, 2007 Data Quality Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified By:.Date: c o o o o o TOXICITY SUMMARY REPORT Test ID Number: 0702-S052 a, b, c, and d Client: Dudek & Associates Prepared: April 3,2007 o o Sample Information Client: Dudek & Associates, Inc. Project Study: Carlsbad Golf Course Toxicity Evaluation - February 2007 Sample Site ID's: a) Upstream Surface b) Downstream Surface c) Well #1 d) Well #3 Sample Collection Date, Time: 2/26/07,13:00 to 14:45 Sample Receipt Date, Time: 2/27/07,13:30 Sample Material: Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Controls: Protocol Used: Test Acceptability Criteria: Test Validity: 2/27/07 to 3/6/07 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, < 8 hours 100, 50, 25, 12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity = 200 pS/cm Lab freshwater spiked with seawater to raise the conductivity to levels matching the samples (3,300 and 5,000 uS/cm). These controls were conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Test results met all test acceptability criteria and internal QA/QC requirements. Table 1. Water Quality Information: Samples Upon Receipt Sample ID Well #1 Well #3 Upstream Downstream PH 6.17 6.73 7.60 7.90 DO (mg/L) 5.8 5.2 10.8 10.8 Temp (°C) 11.2 8.8 7.8 7.1 Conductivity (uS/cm) 3,450 5,050 3,230 2,490 Hardness (mg/L as CaCO3) 936 1,574 966 684 Alkalinity (mg/L as CaCO3) 57 236 215 208 Nautilus Environmental - San Diego Bioassay Laboratory G o o o o o o o o0 o TOXICITY SUMMARY REPORT Test ID Number: 0702-S052 a, b, c, and d Client: Dudek & Associates Prepared: April 3,2007 Table 2. Summary of Test Results Sample ID Concentration (%) Lab Control Well #1 6.25 12.5 25 50 100 Lab Control Well #3 6.25 12.5 25 50 100 Lab Control Upstream 6.25 12.5 25 50 100 Lab Control Downstream 6.25 12.5 25 50 100 Conductivity Control #1 3,300 uS/cm Conductivity Control #2 5,000 uS/cm Mean Percent Survival 100 100 100 100 100 100 100 100 100 100 90 40* 100 100 100 100 90 100 100 100 100 100 100 90 100 80 Mean Number of Offspring Produced 24 22 23 23 17* 8* 23 23 23 22 13* 1 * 24 27 25 25 24 20 24 28 25 27 27 20 18* 2* * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Notes: 1. Conductivity Control #1 had a conductivity level similar to the Well #1 sample, as well as both Upstream and Downstream samples, all which ranged from 2,450 to 3,450 uS/cm. 2. Conductivity Control #2 had a conductivity level similar to the Well #3 sample, which was approximately 5,000 uS/cm. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: 0702-S052 a, b, c, and d _ Prepared: April 3, 2007 O Table 3. Summary of Statistical Results and Reportable Values © 0 O O O O O0 O Sample ID Test & Endpoint Well#1: Survival Reproduction Well #3: Survival Reproduction Upstream: Survival Reproduction Downstream: Survival Reproduction NOEC (% effluent) 100 25 50 25 100 100 100 100 ECso (% effluent) >100 78.2 87.1 56.0 >100 >100 >100 >100 TUc (toxicity units) 1.0 4.0 2.0 4.0 1.0 1.0 1.0 1.0 NOEC = the highest Concentration that produces No Observed Effect ECso = concentration that causes an adverse effect to 50% of the test organisms Chronic Toxicity Units (TUc) = 100 f NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. Survival and reproduction of the Ceriodaphnia were not affected in the Upstream and Downstream samples. The Well #1 sample produced good survival results, however there was a slight toxic effect to the reproduction. The Well #3 sample had a slight toxic effect to the survival, but a much stronger toxic effect with regards to the reproduction. These adverse effects, especially with regards to the reproduction, were expected however, due to the elevated levels of conductivity found in the Well samples. Through internal studies and experience, we have consistently shown that adverse effects begin to show in the reproduction endpoint when conductivity levels exceed 2,500 (jS/cm. Conductivity levels are determined by the varying concentration of salts in a sample. The correlation between conductivity levels and decreased reproduction in Ceriodaphnia was observed in this study as well. The Well # 3 sample had the highest conductivity level (approximately 5,000 uS/cm), and as expected, showed the greatest effect on the number of offspring produced (a 96% reduction compared to the control). The Well #1 sample also showed an adverse effect to reproduction, but not to the same degree (a 66% reduction). Likewise, the two Conductivity Controls, which matched the conductivity levels of the two Well samples, showed similar reductions in the reproduction rate. Both the Upstream and Downstream samples resulted in no toxicity or adverse effects. The results of this study seem to indicate that the elevated conductivity levels found in the Well #1 and Well #3 samples may have contributed to the adverse effects seen with the Ceriodaphnia test species. Nautilus Environmental - San Diego Bioassay Laboratory ! 5 O o O a Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis oo Site: Upstream CETIS Test Summary Page 1 of 2 Report Date: 03 Apr-07 10:18 AM Link: 18-9362-7671/0702-S052a Ceriodaphnia 7-d Survival and Reproduction Test Test No: 01-8184-2754 Start Date: 27 Feb-07 03:30 PM Ending Date: 06 Mar-07 02:00 PM Setup Date: 27 Feb-07 03:30 PM Sample No: 08-1124-3591 Sample Date: 26 Feb-07 02:00 PM Receive Date: 27 Feb-07 01 :30 PM Sample Age: 26h Comparison Summary Analysis Endpoint Test Type: Reproduction-Survival (7d) Protocol : EPA/82 1 /R-02-01 3 (2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Code: Source: Station: Upstream Ambient Sample 07-0134 Dudek & Associates Upstream NOEL LOEL ChV 16-0820-9604 7d Proportion Survived 08-5162-4915 Reproduction Test Acceptability Analysis Endpoint 100 100 >100 >100 Attribute 16-0820-9604 7d Proportion Survived 08-5162-4915 Reproduction 08-5162-4915 Reproduction Control Response Control Response MSDp 7d Proportion Survived Summary Conc-% Control Type Reps 0 (V0) Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 (3,16$) Conductivity C 0 Lab Control 6.25 12.5 25 50 100 10 10 10 10 10 10 10 Reps 10 10 10 10 10 10 10 Mean 1.00000 1.00000 1.00000 1.00000 1.00000 0.90000 1.00000 Mean 18.1 24.111 27 24.7 25.111 24.4 19.9 Minimum 1.00000 1.00000 1 .00000 1.00000 1 .00000 0.00000 1.00000 Minimum 11 13 25 15 16 14 12 N/A N/A Statistic 1 24.1111 0.2413 Maximum 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Maximum 23 32 29 29 31 33 28 Duration Species: Source: Client: Project: MSDp N/A 24.13% Acceptable Range 0.8 - N/A 15 -N/A 0.13-0.47 SE 0.00000 0.00000 0.00000 0.00000 0.00000 0.10000 0.00000 SE 1.3940 2.2326 0.4216 1.317 1.7752 2.1406 1.7540 SD 0.00000 0.00000 0.00000 0.00000 0.00000 0.31623 0.00000 SD 4.4083 6.6978 1.3333 4.1647 5.3255 6.7692 5.5468 Nautilus Environmental (CA) : 6d 22h Ceriodaphnia dubia In-House Culture Dudek & Associates Method Fisher's Exact Bonferroni Adj Wilcoxon Rank Sum Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria cv 0.00% 0.00% 0.00% 0.00% 0.00% 35.14% 0.00% CV 24.36% 27.78% 4.94% 16.86% 21.21% 27.74% 27.87% 000-089-125-1 CETIS ™v1.025B Analyst:.Approval:, CETIS Test Summary Page 2 of 2 Report Date: 03 Apr-07 10:18 AM Link: 18-9362-7671/0702-S052a 7d Proportion Survived Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Rep1 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 2 1.00000 1.00000 1 .00000 1.00000 1 .00000 1.00000 1 .00000 Rep 3 1.00000 .00000 .00000 .00000 .00000 .00000 .00000 Rep 4 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 5 1.00000 N/A 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 6 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 7 1.00000 1.00000 1.00000 1.00000 N/A 1.00000 1.00000 Rep 8 .00000 .00000 .00000 .00000 .00000 0.00000 1.00000 Rep 9 1.00000 1.00000 1.00000 1 .00000 1.00000 1.00000 1.00000 Rep 10 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Rep1 15 13 25 15 17 18 14 Rep 2 21 24 28 28 24 14 28 Rep 3 11 28 28 26 28 19 13 Rep 4 23 31 25 23 26 30 12 Rep 5 19 N/A 29 26 31 29 17 Rep 6 21 32 27 28 16 33 24 Rep 7 14 24 26 25 N/A 29 23 RepS 22 24 27 29 26 17 22 Rep 9 13 27 27 21 28 30 25 Rep 10 22 14 28 26 30 25 21 000-089-125-1 CETIS™ V1.025B Analyst:.Approval:, CETIS Analysis Detail Comparisons: Report Date: Analysis: Page 1 of 1 03Apr-0710:18AM 16-0820-9604/0702-S052a ) Ceriodaphnia 7-d Survival and Reproduction Test 1 5 Endpoint Analysis Type , ^ 7d Proportion Survived Comparison , » Method Alt H Data Transform Fisher's Exact C>T Untransformed Group Comparisons * ) Control vs Conc-% Statistic Critical f\ Lab Control 6.25 1.00000 0.05000 12.5 1.00000 0.05000 f) 25 1.00000 0.05000 Q 50 0.52632 0.05000 100 1.00000 0.05000 Nautilus Environmental (CA) Sample Link Control Link Date Analyzed Version 18-9362-7671 18-9362-7671 03 Apr-07 10:16 AM CETISv1.025«NOEL LOEL Toxic Units ChV MSDp 100 >100 1.00 N/A Decision(O.OS) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Data Summary ^ Conc-% Control Type Non-Responders Responders Total Observed |P 0 Lab Control 9 0 ^ 6.25 10 0 ^ 12.5 10 0 £25 9 0 50 9 1 W 100 10 o W Graphics ™ Uh O O O O O £ 0.9- O A 1 °'8 ™ | 0.6- • | ^• s ft4i • 2• " 0 6.25 12.5 25 50 100 A Conc-% 9 10 10 9 10 10 000-089-125-1 CETIS™ V1.025B Analyst:.Approval: CETIS Analysis Detail Comparisons: Report Date: Analysis: Page 1 of 1 03Apr-0710:18AM 08-5162-4915/0702-S052a Ceriodaphnia 7-d Survival and Reproduction Test Endpoint Analysis Type Reproduction Comparison Nautilus Environmental (CA) Sample Link Control Link Date Analyzed Version 18-9362-7671 18-9362-7671 03 Apr-07 10:16 AM CETISv1.025 Method Alt H Data Transform Z II NOEL Bonferroni Adj Wilcoxon Rank Sum C > T Untransformed 100 ANOVA Assumptions Attribute Test Statistic Critical P Level Variances Bartlett 18.80485 15.08628 0.00209 Distribution Kolmogorov-Smirnov D 0.16362 ANOVA Table Source Sum of Squares Mean Square Between 273.736 54.7472 Error 1447.178 27.83034 Total 1720.91376 82.577545 0.13527 0.00052 LOEL Toxic Units ChV MSDp >100 1.00 N/A 24.13% Declsion(O.OI) Unequal Variances Non-normal Distribution DF F Statistic P Level Decision(O.OS) 5 1.97 0.09907 Non-Significant Effect 52 57 Group Comparisons Control vs Conc-% Statistic Critical P Level Ties Lab Control 6.25 113 12.5 101 25 90 50 104.5 100 80 Data Summary 0.8423 4 0.5159 3 0.6348 4 0.6099 4 0.0564 4 Original Data Conc-% Control Type Count Mean Minimum Maximum SD Decislon(O.OS) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Transformed Data Mean Minimum Maximum SD 0 Lab Control 9 24.111 13 32 6.6978 6.25 10 27 25 29 1.3333 12.5 10 24.7 15 29 4.1647 25 9 25.111 16 31 5.3255 50 10 24.4 14 33 6.7692 100 10 19.9 12 28 5.5468 Graphics 40- r I * i i •i,| | i 10- 0 6.25 12.S 25 50 Conc-% 10- s- -5- -10- /•60 ° jf£> j^OSJT/ ^X°°° 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Ranklts 000-089-125-1 CETIS™ v1.0258 Analyst:.Approval: o o Freshwater Chronic Bioassay Client/Sample Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Dudek & Assc./ Upstream Start Date/Time:7/-UM-7 \S3O End Date/Time: •?. • t*l Oo Cone. ,<* Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup K ^iH i^ iv* VM( (V* \if \H yOk V^J OH i# 3M 1A *<fe H<\ <;* 5« SH /V^t- <;w- Daily Reproduction/ Survival 1 ". o <o C? <p#£> or <? o o o ^ 2 0 o 0 o . — 0 b o 0 o Sfr '3 ,' 0 0 0 o — o oo 0o f»T !fl'^ o 5 T "7 — £ 5" -7 / H- *~T. ;,•"«'H «D 0 o — O 0o 0o "^ "6" 1a I*.l» — 19, "» 1 0 t( to fcu '"7 o ^-l, ^u £TlA — *r*y 10i 10 O *^ 8 - Total n•ZH T^f 31 L±? 72- 2-W 2^ 1-1 /I QC \2 SW Cone. Cc*v . Rep 1 2 3 4 5 6 7 8 9 10 '"" ' i i Daily Reproduction/ Survival 1 •o {? O O O C? oo C3 O 2 0 C O 6 6 6 6 ft 0 O 3 O o O p 0 0 4 Q O P 4 0r G> 5" s s 3 7 6 5 5," 5 OA D o 0 5 Po D •; e1 o u>1% e i 6 1'i t> 7 i D 10 O to G> ~7 O "tou *l 9 Total /r Z-(/f ^3 If 14 /H lx- /3 1-L, QC 10 3^ Cone. (0?. Rep 1 2 3 4 5 6 7 8 9 10 , ' ' ' > ' Daily Reproduction/ Survival ,1 o o O O 3=rH>- •o O o o o 2 ' O 0 o 0 o o o 0 o o 3 o a a 9 O do 0 " '4''"1 C r A t 5 6 r Atf 6 ,'• 5" .;, D O O O o o 0 o 00 '•••0-',1 -n ip-•i> ui>\\ l-o 10ii ^ 7 i I ii 10 \o \ 2*to \\II\d- Z-T 2-1 •ztf QC \Ai^ | Time Fed (day): (0)(3) \U5 Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Cheek:Final Review: Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. 'Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna ^Client/Sam •Test No: 0 Time Fed (da 0 Notes: d = d ole ID:Dudek & Assc./ Upstream Start Date/Time: 2/27/2007 1S3O 6'To^ ~So5~y^a^ End Date/Time: '3» Vo« o~l l<Ho£ Cone. 12.5% Analyst Rep 1 2 3 4 5 6 7 8 9 10 NM Daily Reproduction/ Survival 1 o <o £2 o ooc o o <0 f-> 2 0 0 0 0o o 0 O 0o &\\ 3 o o O O Q. O Q n n p v-^ ,4 35" S f S £ f S r~3- 5 . 4, r\ o 0 O D 0 o A ?fs 6 ii » 3- AO to q \ 0<^ \ 1 ??\ 1 •V U 7 o 1 O ^ <-o 1 ; (O- \l 1 a"1 "1 > «cs lAL- 8 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 oc> 0 0 <? oo 0 oa z noo 6 0o o 0 6o 3 , Q Oan n 0t^ O n o 4 d H <^ $ k-3P/< 5- T 5 , "7 o Of>, no — £> O O 6 t 0 t o \ 5-u \3 H —i 3- l\ is- 7 n[c> I ( I 9- \ *• O ~- t\ i3 1 9^ 8 — Total IT 2£2,^ Z"J ~L,lf "2JS -L'Z TJ\ 2-( Tf^f QC GZ-ift Total n 'iM ~L5t /; / 3i K/ t-£.f iJL iM "lo QC l\Sfr Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 o o oO O O o Oo o 2 0 0 0 6o fto ft 0 0 3Q Q O O 0 Q o Q O 0 4 0 H "l S" •?• c- T ^fe 5 • 5 ' ' to O n O o ^0 O o d ' 6 i>" I 0 i 9- i ^ i"^ ilii- 1 I/O (:>• <i;i • T O 0n\~$ \\( u\\ y— I 9- l 1 8 — Total 1%/f L^ *3o 1$.33 •z-1 1*7 /4 7&z-r QC V\ •SW Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 v): (0) ^D (1)10 sad; M = male; LIP = Daily Reproduction/ Survival 1 o o o o o 0 oooo £±_(2 lost in f 2 <5 0 fto C) o oo 0 (7 3 0 q O Q 0 O Q Q Q ffc 4 Q t> o ^u ^M- H•>' ^ 5 ' <-) o «t ^Q O Ow o O 6 (5 U % $ G" * -1 6 ^ft tt^O (3) Va*! (4) \*ot (5) uogress; B = 4th brood (only the first 3 7 i<s i i (2 c-> ft i O ^p. i*O t \ 'o 8'.:.;;::, N-fo (6)^7-0 broods are inch Total iH TJ* 11 12.(~j 2^1 7-*^ T-T, Iff ~la\ QC ta'Stt- (7) (8) jded in total) 0 Comments: ^ QC Check: #£/ Vtt'^Final Review: #^- ^i-1^^^} Nautilus Environmental, LLC. 5550 Morehouse Drive, Sotte tSO. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client Samp TestN Analyst Comme Animal QCChe elD: o: Concentration Day_ Oudek & Associates Upstream O~l&'?~- SoS~A-o^_ Test Species: Start Date/Time: End Date/Time: C. c/uto/a 2/27/2007 153>0 t5 -(^ O~T /^OO Lab Control -** 1 0 1 2 3 4 S 6 7 Initial PH DO (mg/L) Cond. ((jmhos/cm) Temp (°C) g. I ^ g. 3 ^30^ &y.5 ^-iH *". I HH i^". "3 S.v* T-.& l°l^> zt5. o «.5.M S.H V°\M 25. \ « . \"^ ©. 0 \°13 ^•5 3 S.A3 lp~7 /*}/ «^S"/ ©,30 ^ ^5) O aH.^ Final PH DO (mg/L) Temp CC) 7.<S§> 9- H Q5^ 8.<^> g>. | 95,o •^j ^•^ "ZA/I ^>7 -^ ^^H-i ^"t>B 8T.O A5T3 ^r01 * g,^ •2^5>'O •g.O <£> •g g 3M.^ Concentration Day Conductivity Control * 1 (3300} 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (jjmhos/cm) Temp (°C) "7-9T 5.O ij^o ato. o ?127 y- / lrJZ.0 "2-5. M ©. 7t "^ .°1 '^>^ic C 2-5-N a. 14 «-S 3^20 2.sn ^ . l\ ft • v S5Cpt a-'S."^ ^i d°> ^•"7 > 33Wo -2S- V •€>-\~i *q..^v =,-2.5° SM..5 . Final PH DO (mg/L) Temp (°C) "7 Ao Q.3, ^6.3 *7.*?H e.a J>5. o *T.1f <g.t*. 7, O 7-2>*3 9j?. ^ ,2^,^ Concentration Day 6.25% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp CC) f.1 1 <fr 0 5^1 s»^.f> •2-7. "\ tf -^'mi ^H.•^ O.L5? "V -°) M^,5 "2-5- >O 3-Z-2. ^<? MK 2.*\,T 8-\^ •a. 2- H^V^ ^•5 -t, Q,\^ ^ . O 0, 0^ 9-5 >A 8.3°^ -A - "^ M3T, 5-H.^ ,,. : Final . , . -. PH DO (mg/L) Temp CC) s: Initial: Final: nts: Source/Date Received: ck: ^ -J-t* §>.0( •& .^ '^'b. 1> 8^01 &. i 5^ O 1?*^£ ^.lf -xx f Q-oCs ©A sH^ "? Jc? 1^.D 5&^ g.o v !^, -2^ ->6O £?oS •S..-6 3V&> 01234567 £L |^\.(- \^ L»^ ^-^ ^U su r~^ Z&r "^^- *-> >4 12-A |^3 ^0 •^if\ Internal N/A Animal Age at Initiation: Final Review: ^^kO /Z^ Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client: SampU Test N< Analysts Common Animal S QC Chec »ID: 5: Concentration Day Dudek & Associates Upstream cn&s^ ?° 5~2-«\. Test Species: Start Date/Time: End Date/Time: C. dub/a 2/27/2007 \6'^^> •*,.(*•£>-} (ctOO 12.5% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) "7.g>R 6. o Lo^>Lf 3^,«l €.U 8- A £ $ ^ •xH-^ •S-lw % , t> ui"?> pH-"1 •8,V« •».< tl-7 Z'Vt S.V"2> 6. ^> VO& 03 as .-5 &. \T_ @>- 0 {& "2-^ 3-6..UJ •g-2^ •^ -^ Cot-l U 5^-(.O5 Final PH DO (mg/L) Temp (°C) <&. " ie>-H^^.3, ?>./•<? %.^ - 3^.o ^o5 -*fv^ ^X^| "Vl'S *© -\ sH-^ ^-V~1 'S)^ c5^S3 6' ° fe. O Oc5.0 7. T5 X5 — T 2!w. ^a Concentration Day 25% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) 7.7 1 fl S \^^<f 3-V.& <j; otf ^..1 \\IC-, L-H.3 "Q.oM <23.-L to$^ OH .•& •8*n «,« V0*2 Z*\,S y -o^ ^v^\VL»£> -s-^.-u TsS-csH <e»-T. •HSrO^l. •3>v«o @. 1 ° ~3 8 10^ "> ^M-Ca Final PH DO (mg/L) Temp (°C) <B-O*7 £,.f/ A'&.S fe- 1 ^ S- D 5t-.o ^•^0^7 tf.H 'l-5.i 8-0 o 8- \ JM ^ *? ^-l ^i-O 5^, ,-2, Q>.o-=i rt 9<5.O ~7. 13 C». 0) ?^7 ^ Concentration Day 50% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) 7-73 8.5 IT&5 2.L/ -7 7-8=1 ^?.7 V\^' T-i-\.^ •*- . 8M 8-5 fl^-O 5"4-^ 1^& *V.2 V%~lS in?- (g. 00 ft. 5 2-£3 ^o o ^.^.\ ^op? ^>^:7 ^<^o^ ^"5>.^ "^•n ^ ^>°> \'@ 2, 3 &M- "^ . Final ; PH DO (mg/L) Temp (°C) 1S).|-7%.*y ^^ 3 8. -94 §>-y- 3-6.v tt ~, a if "J-Js.t 8- tS> 9>. \ dM-^; ^9^1^o 5,S^ j^ "\Of 4J\ "6-\ 5-5 -O ~7.^T ©,O> ^C/.^jA Concentration Day 100% 0 1 1 2 | 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) 7.£,/ f. s ^ac'o Si^C, T7< ^5 ^. S £" J» O *TlLiLL 1~1- M "V. \ 3^i.^>0 9H-5 l.nt «\.« "^ZOD Z^,\ -t). .^L/ 8.8 %"2.WO SM.^ •^ 8? Q.Q ^,T,^-o ^-^^ ""fS ^ ^ >- *~? ^^ £--Ci O >H- ° a H-TJ Final PH DO (mg/L) Temp (°C) : Initial: Final: ts: ource/Oate Received: /k: fa 1~1$-« "^-i j ^.^ 2r3."!> fe H^ 8.0- ^.0 ^Li^^ ?.«5 V>.f © . Tg) ©•0 d-t°l S ^^, O f^J «^ *i ^Sc&'p @, T_ sS.'O ^*"S is •a.--/SYS 01234567 \CL r^T> {C-Lx R-3 cu S\\ i^2I r^ )S_j R-^ ^r^ R.O ^-^ ~\ Internal N/A Animal Age at Initiation: Final Review: ^awo g^ Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. oo Site: Downstream 0 O CETIS Test Summary Page 1 of 2 Report Date: 03 Apr-07 10:51 AM Link: 09-5603-9470/0702-S052b Ceriodaphnia 7-d Survival and Reproduction Test Test No: 04-1879-4777 Start Date: 27 Feb-07 04: 1 0 PM Ending Date: 06 Mar-07 02:20 PM Setup Date: 27 Feb-07 04:1 0 PM Sample No: 02-6416-9233 Sample Date: 26 Feb-07 01 .00 PM Receive Date: 27 Feb-07 01 :30 PM Sample Age: 27h Comparison Summary Analysis Endpoint Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013(2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Code: Source: Station: Downstream Ambient Sample 07-0135 Dudek & Associates Downstream NOEL LOEL ChV 00-4213-7562 7d Proportion Survived 18-9845-2640 Reproduction Test Acceptability Analysis Endpoint 100 100 >100 >100 Attribute 00-4213-7562 7d Proportion Survived 18-9845-2640 Reproduction 18-9845-2640 Reproduction Control Response Control Response MSDp 7d Proportion Survived Summary Conc-% Control Type Reps 0 ft/i<>») Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 A"J6<0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 10 10 10 10 10 10 10 Reps 10 10 10 10 10 10 10 Mean 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.90000 Mean 18.1 24.111 27.7 25.222 27.2 26.8 20.4 Minimum 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Minimum 11 13 15 14 19 22 4 N/A N/A Statistic 1 24.1111 0.23853 Maximum 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Maximum 23 32 35 30 30 30 27 Duration Species: Source: Client: Project: MSDp N/A 23.85% Acceptable Range 0.8 - N/A 15 -N/A 0.13-0.47 SE 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 0.10000 SE 1.3940 2.2326 1.7 1.7856 0.9866 0.8919 2.125 SD 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 0.31623 SD 4.4083 6.6978 5.3759 5.3567 3.1198 2.8206 6.7198 Nautilus Environmental (CA) : 6d 22h Ceriodaphnia dubia In-House Culture Dudek & Associates Method Fisher's Exact Bonferroni Adj Wilcoxon Rank Sum Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria cv 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 35.14% CV 24.36% 27.78% 19.41% 21.24% 11.47% 10.52% 32.94% 000-089-125-1 CETIS™ V1.025B Analyst:Approval: o o CETIS Test Summary Page 2 of 2 Report Date: 03 Apr-07 10:51 AM Link: 09-5603-9470/0702-S052b 7d Proportion Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Survived Detail Control Type Conductivity C Lab Control Detail Control Type Conductivity C Lab Control Rept 1.00000 1.00000 1.00000 1.00000 1 .00000 1.00000 1.00000 Rep1 15 13 15 14 28 29 22 Rep 2 1.00000 1.00000 1.00000 1.00000 1 .00000 1.00000 1.00000 Rep 2 21 24 35 25 27 24 22 Rep 3 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 3 11 28 25 28 29 28 21 Rep 4 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 4 23 31 31 30 27 30 19 Rep 5 1.00000 N/A 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 5 19 N/A 30 26 28 30 18 Rep 6 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 6 21 32 30 30 19 26 18 Rep 7 1.00000 1.00000 1.00000 N/A 1.00000 1.00000 1.00000 Rep 7 14 24 26 N/A 27 29 27 Rep 8 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 8 22 24 28 28 30 26 26 Rep 9 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 9 13 27 26 19 30 24 27 Rep 10 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 10 22 14 31 27 27 22 4 000-089-125-1 CETIS™ V1.025B Analyst:,Approval:.X o ® CETIS Analysis Defe •0 0 • • 0 9 0 ^ • •0 f A Comparisons: Page 1 of 1 .. Report Date: 03 Apr-07 10:51 AM 3" Analysis: 00-421 3-7562/0702-S052b Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint 7d Proportion Survived Method Fisher's Exact Group Comparisons Control vs Conc-% Lab Control 6.25 12.5 25 50 100 Data Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Graphics l.O-i O O 0.9: | 0.8J M 0.7: 1 0.6- * 0.5- •0 0.4- 0.3- 0.2- 0.1- 0 6.25 Analysis Type Sample Link Control Link Date Analyzed Version Comparison 09-5603-9470 09-5603-9470 03 Apr-07 10:47 AM CETISv1.025 Alt H Data Transform | NOEL LOEL Toxic Units ChV MSDp C>T Untransformed 100 >100 1.00 N/A Statistic Critical Decision(O.OS) 1 .00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 0.52632 0.05000 Non-Significant Effect Non-Responders Responders Total Observed 9 09 10 0 10 9 09 10 0 10 10 0 10 9 1 10 0 O O o 12.5 25 50 100 Conc-% 000-089-125-1 CETIS™v1.025B Analyst:Approval:. o 0 •• • • • •• • • • • •• •• •• • • • *• • ••• • •m CETIS Analysis Detail Ceriodaphnia 7-d Survival and Reproduction Test Comparisons: Page 1 of 1 Report Date: 03 Apr-07 10:51 AM Analysis: 1 8-9845-2640/0702-S052b Nautilus Environmental (CA) Endpolnt Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 09-5603-9470 09-5603-9470 03 Apr-07 10:47 AM CETISv1.025 Method Alt H Data Transform Z | NOEL Bonferroni Adj Wilcoxon Rank Sum C > T Untransformed 100 ANOVA Assumptions Attribute Test Statistic Critical P Level Variances Bartlett 10.30154 15.08628 0.06713 Distribution Kolmogorov-Smirnov D 0.17186 0.13527 0.00020 LOEL Toxic Units ChV MSDp >100 1.00 N/A 23.85% Decislon(O.OI) Equal Variances Non-normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Decision(O.OS) Between 368.9763 73.79525 5 2.71 0.02978 Significant Effect Error 1414.144 27.19509 52 Total 1783.12067 100.99034 57 Group Comparisons Control vs Conc-% Statistic Critical P Level Ties Lab Control 6.25 116.5 0.8945 5 12.5 91 0.6668 5 25 113 0.8423 4 50 108.5 0.7255 5 100 83 0.0912 4 Data Summary Original Data Conc-% Control Type Count Mean Minimum Maximum SD Decision(O.OS) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Transformed Data Mean Minimum Maximum SD 0 Lab Control 9 24.111 13 32 6.6978 6.25 10 27.7 15 35 5.3759 12.5 9 25.222 14 30 5.3567 25 10 27.2 19 30 3.1198 50 10 26.8 22 30 2.8206 100 10 20.4 4 27 6.7198 Graphics 40-j 1 S 30" 11 ,1! Ml| 20- 1 9 3g -K a -i 10- -1 H ^ */*<?'. o s: : o 0 6.25 12.5 25 50 100 -2.5 -2.0 -l.S -1.0 -O.S 0.0 0.5 1.0 1.5 2.0 2.5 Conc-% Ranklts 000-089-125-1 CETIS™ V1.025B Analyst:.Approval: Q o o Freshwater Chronic Bioassay Client/Sample Test Number: Dudek & Assc./ Downstream Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 2/27/2007 End Date/Time:a? O C • • • • • • • • •• •• • •• •• • •• •_ Time Fed (< 0 Notes: A Comments QC Check Lab* Control 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: f^f- 'Hl*t "-/ 11 M '*"1ju_ 12. IZ_ IT- l/«f 2>f ZJj' S1-^ w 41tg 5^- 17. ^ f T Stt Cone. Conrf^ Control hiw $/ Rep 1 2 3 v 4 ' 56 7 8 9 10 <0 O a- o i_\P 0 o C o -o £— ^ 6 0 0 0 ~ 0 D 0 0 0 "fcfrSt* oo o o ~- £• O 0 O <0 ^.i- o 5 "^e"^S ^"^— 6s1 7 6 M- .-^i "S^0! 3 O o L> - 0 u o o o ^b> £\ fe ts^ V3- - 13 ** lo 11 IO Y.V- a \\ vv Vi - in U7 T 10 nr*-_^ ^ Daily Reproduction/ Survival i o o O o O o o o oo 2 O o 0 0 0 0 0 0 0 0 ", 3"6 o £) nDat>oo £> 4-'.." 0s or s 5 1 3 / <$ '. '$.!,!''(^ 0 4 O 0 o s e> 0 D '':r'i.''l&,,' ^IP T£ ft *\ <& ^-7 C> .: 7 to vo o \n / T 6 \o 0 <\ 8 |3 2/^< •?& 31 Utt "3>T- -i/-\ 2-^ "2.1 /I Viift Total ^5" "Z-\K 1J> 1*1 *^xl I(J VI, Hin^ QC 10 ift Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 toy); (OH,'0 (Djg d = dead; M = male; /, ,.,. iSl LIP Daily Reproduction/ Survival 1 -D G O O <o O f-y O O O 2 O 0 0 0 6 0 0 o t) 0 3(3 O "v"^ lo H T ^\ -\ <^ 7 <±> v°\ U \~L-\°v\"i\^> " L")-^i<- "VAtS* 8 i \ the first 3 broods are in il5tal f ^ '-^8? l! ^JT ^5 ^5" 31 ^ 3o "X73«'^^4^ ~l£ ~3l QC ^^u ') m eluded in total) -<J~? Final Review: £z- ^-^"<^7 Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Q O freshwater Chronic Bioassay ^Client/Sample ID: 0Test No: Dudek & Assc./ Downstream Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna _ Start Date/Time: 2/27/2007 End Date/Time: >> v/- ^ Time Fed (da £ Notes: d = d Cone. 12.5% Analyst Rep 1 2 3 4 5 6 7 8 9 10 i^i Daily Reproduction/ Survival 1 e? C 0 Q 0 O (i P £•' 5' O fk.S 2 O o O 0 0 o — oo o svv 3 6 V L_ £> 0 O V —t> £> O \U— 4 6 £L •5 / ^f •^ M- 4 ^VJ. "5 ' o (P O 0 o 0 — 0 y o <=-3* '' '6 ^1 iiu 1to — \ 1 1 (0 I/I . 7 u \-v < 1^ •\"2, \1' 1U> — I?P X) \ \ U s 8 — Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 Cc t •o o o o c,1 o C 2 a 0o o o 0 0 o D o w &oo ftof}u 0 o 0 4 SH ^_59; ~7 % Ar4- "'".£'/-) o £J Oo 0 0> t> 0 o §' ' fo Co I f \o l«3 i *• VI U C( ^ ,' 7 ' 1*5? \"3j I"2) \1^ \O O \l ^15 i<4 ;•• s ' Total /iy ^,•5- 2^" 3o Z^C, 3t> LXP Z^? Ci ~L~I QC \2. ilV Total Z4T TS\ ~lf[in "2&11 2,-1 30 3/) In QC \t Srt Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 co cc r? 0o o o o 2 o o f> 0o o 0o 0 o ' "3, ; L> o O D<o &u& V 6 •4 •- f) S 1 -7 <; 1 7<£ <6 i ,'$', ''o o /) a oo Oj 0 o ''; 6 (O *\\ \ ^3. l(n\<? <\ \t? io '' 7 v"5 |O 1° \ ^(M \ ^IVvv °\ C_p 1 ;!Total •2,' 2/1 1* 3° 7° 1A* 1fi\ TA. 1>\ 7r~Lr QC vo <>w- Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 c V 0o V V u I/' t< •o y): (O)K(Q (DtiW (2 ead; M = male; LIP = lost in f 2 o oo o o 6 ft 0 o 0 3 d) £> f £J>^JL> t> Qt)O '"4 6 ^V •J t- $ f, 7 if ^\ "5"'! <3 v-7 fPOo (3 10 4J O O '• '6 1 i ofi fc-7 5 0 \t> to O/ C7 t,DMS (3) iu/c> (4) IU00 (5) | >rogress; B = 4th brood (only the first 3 7 *^, ^A^ &4 V^)l\ °) i •*> \ 8 .— Total "Z-Tx fl— -z.\ 1^(] U 2-n 1^> 2-"1 H /i(f broods are includec QC « sVt 71 (8) 1 in total) A Comments: *QC Check: K- •J-VJ-CT) Final Review: >^ ^-i-trf 'Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92*21 Q © Fresh Client Samp TestN o ©o •• •• • • • • • •• • •• •• • • • • ^^ Analyst •^b Comme •A Animal £ QC Che £ Nautilus • • • • water Chronic Bioassay : Dudek & Associates elD: o: Concentration Day Downstream *7oT- -rdS-24 Water Quality Measurements Test Species: C. dub/a Start Date/Time: End Date/Time: 2/27/2007 \UtO 3.1^.^-7 ,H^O Lab Control •*• I 01234567 initial a. ,~, PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) 8>.\o 0.3 Sex? 2n3 «^M ^-1 I If "L< "i %>.D"J T,<fe l*V^ 2-5. 0 *-^* «.H m ZSA ^.i-^ xcrr^.o \o\V6 >6 ."i s.«?^x f """"7 1 CJ( 1 ^^ £^ ^J "&-^o "7.M 5>-lo 9M."»> PH DO (mg/L) Temp (°C) ^C?-- Ci_* ^-^ c5 6-3 ^>5.3 •S.O3- e> .v 2^-° stiSS1'* fv-T z^t ^0-"^ •%ro^ ^S.-^ ^•. °S t.Ci ^5 3 ~>.e\( 6.0 ^52? <&-cte 8- "6 gLL{ /& So -3*9 Concentration Day Conductivity Control *) (3"SCO^ 0123 4567 Initial ,, PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) •7.£j«\ o0.o •y^&o ?L,.0 ^. 2-"? <^. \ 3>'2-0 2.5- S igj.^t, T.^ 3>3^° 3-$. ^ €At <, 4.S 3320 isn ^.•O-4*1 <crJ^S/ tJ3>^° >5 ?*Av §. o<; Cor-, •^0,1)0 ^S-1-/ &.(6{ -7-1 ^*bO QM-5 ~,A° Flnahos- pH DO (mg/L) Temp (8C) "r.QJ^ •^.^ ^6> "* l.'lH !£* • /^ /I pjt C' -^7-7.^^ ^H^»^ -iX.\ S.^sO dlSO 7T1 %.c> eZ-63 "7. C5^ ^- °S^.-o "?-'2><^> S-"cb 2<Y.€> V^'0" Concentration Day 6.25% 01234567 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) 8 C1^ 6-3 ^^o•2 ^r. « ^.1^ 7 ^ l*^^ T- Si fe.l"^ fe-T- •?> Us =? 3M.T •*,z<, «•! 3^5 2H.5 8.>^ •B • "^ >>M°I pH.«fj %• 2.0 to- *» S'fto ^"5% S>. ^3 *^-^^^ <^=, ^M °\ Final PH DO (mg/L) Temp (°C) s: Initial: Final: nts: Source/Date Received: ck: $L 7-T*- S.o3*1 ft. 5 ^>4-2 g,.oo >. «2, =>&.o ^-(?« ?-C T^5 ^ "*-W^i ^f •^ ^*1 ^^»J ^*\ 01234 I/ 1LX C-*f\s\ J \^ /.— "^^ \>u ^H ^v. p,3> ••^^ "7.^D 9"^ ^6 3 "7. ^ "7-f2> 0)6- C? 7B'/ g, (^o 2H ^ 567e^ ^S J^\ ^u.Y*~ •0 Internal N/A Animal Age at Initiation: Final Review: <e)L^S /- *r-V-T Environmental, LLC. 5550 Morehouse Drive, Suite 150, San Diego, CA 92121. * Q Freshwater Chronic Bioassay Water Quality Measurements £ Client: Q Sample 0 Test N( • • • •O ©© 0 • • • • • • • • • • • • • • • • • • • • • • ™ Analysts • ^ Common _ Animal S A QC Chec gfc Nautilus E • !lD: •>: Concentration Day Dudek & Associates Downstream Test Species: Start Date/Time: C. dub/a 2/27/2007 V(^t^ <97^2- —fc^~i-\> End Date/Time: 3- Ijj* o~f l<-O_C? 12.5% 0 1 2 3 4 5 initial PH DO (mg/L) Cond. (iamrtos/cm) Temp (°C) PH DO (mg/L) Temp (°C) Concentration Day S.o3 &3- £,01 ~2,*y. O ^.1_'2- t-Q S'i'? "L.^.7, SS-oS Q.1-* i>^-3 €>.O,'Z- S -i- •5M v ^>v4r\ 6-oS 0.0 ^•5. 0 «,21 ^•1 S"7« 2H,S <B-vO 6- 1> *5^ 3S-1-/ ft .n•o .-* M^v^ S'TJ^ 6 7 -@>-X<P "*""^ 5-2TZ- 9M6 Final ^^^vi 1^x( "tt .•o\ <S-o OH-^ S-O"^ ^.D r&S 3 "7 A3 *7. ^ 36-^ ~T.<:3f5 ft.-r2<-{ <a 25% 0 1 2 3 I 4 5 6 7 Initial PH DO (mg/L) Cond. ((jmhos/cm) Temp (°C) PH DO (mg/L) Temp (°C) ~j 4 1 <?-? 3 <&2c> 3£.o Si.\ ^ ^. !• •RA1? T-S-i "S-v^ 8- "^ ^^ vT<A.V^ •e.\3 3.9 ^vHS 2H-^ ft.n e>. %£TAO S1?, 5 <fe.lz- Cp .°l A.V^V O.s^ ,O •&-Z.O-tf.l^ "Szw 9H-1 Final S-ol "&.H ^6.1> %.° \&. t p^o •g.TA ? *5i-5. ! fe-^ \M Q. 0 •3-1. °\ oA^, "5;\ Sl^,3 ^.^T -7.6 r3^- O -7.&-1 S>.'7 ^-'S Concentration Day 50% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp CO 7,«t f & $ (?>$( P^.t? •X.o? *?-^iqiq T-<T.O & -"^M <^> . 1 IMtO •>M. z s.f>3 1-3 V5C\ ZHA ^>-\"t s . ^ lM3"l u^ ,\ 6.o<^ €-u> \iA^\ o 35^ fi. 1 °•p,.^ fS^H S>-\.-3- Q.o<t> Final PH DO (mg/L) Temp (°C) fe.53g,.ty ^3^ "\ g.((pr^ 8-2 3>5o •?_Z3y. sX.'O ©.-7,8 Q - \ >4,°i ' ?A>'^> "^,^5. r?.6 <2, 6>.i^ l.^ 36 o 'g.o'S. S .^P-q <3 Concentration Day 100% 0 1 2 3 | 4 5 6 7 Initial PH DO (mg/L) Cond. (Mmhos/cm) Temp (°C) •?.£>€> *r *»2^70 TJy.C? -j 0(L^ C\.L\ '''HfcO •VH.^ "1 ?IT. 'I- 1 3MCoO >^'° HAS •\-i ZH^O 2HA 8-o-p, 6-1 >MUO >^. © ^-O^ ft-^ ?^l^0 0^^ T^l^ C> "=^ 3- l^-) C-^ O a-M-o Final PH DO (mg/L) Temp (°C) Initial: Final: ts: ource/Date Received: k: /L 7 -"&£-* •&-M3- g.l-/ Q^.^ '6.'^^ 6-V 3.1^.0 0 1 2 ^ (VvT Y-Ls U-^» ^u zf-li?^-4 >S.I S.?>^ ^•O 3M..°\ 3 4 sw 7X.J 12-^ &-.:> Sr2^>'S-O- i2^2> 5 R~i ^iK ^. 3>l "?. <-6 ' ^t^.e? $iJ2,(0 ^-f^V.'ZS 6 7 F~^ ^6-^<^' •7 Internal N/A Animal Age at Initiation: Final Review: <4WS /^ nvironmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. o © Site: Well#1 CETIS Test Summary Page 1 of 2 Report Date: 03 Apr-07 11:22 AM Link: 08-4701-8123/0702-S052C Ceriodaphnla 7-d Survival and Reproduction Test Test No: 09-3605-9635 Start Date: 27 Feb-07 03:25 PM Ending Date: 06 Mar-07 02:00 PM Setup Date: 27 Feb-07 03:25 PM Sample No: 07-2146-8492 Sample Date: 26 Feb-07 02:45 PM Receive Date: 27 Feb-07 01:30 PM Sample Age: 25h Comparison Summary Analysis Endpoint Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013(2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Well Water Code: 07-0136 Source: Dudek & Associates Station: Well 1 NOEL LOEL ChV 09-0754-8491 7d Proportion Survived 1 2-8266-2273 Reproduction Point Estimate Summary Analysis Endpoint 09-0768-6815 Reproduction Test Acceptability Analysis Endpoint 100 > 100 25 50 N/A 35.355 % Effect Conc-% 95% LCL 25 44.50000 30.06757 50 78.24074 61.55914 09-0754-8491 7d Proportion Survived 09-0768-68 1 5 Reproduction 12-8266-2273 Reproduction 12-8266-2273 Reproduction 7d Proportion Survived Summary Conc-% Control Type Reps 0 fi^oo") Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 fj;700 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 10 10 10 10 10 10 10 Reps 10 10 10 10 10 10 10 Attribute Control Response Control Response Control Response MSDp Mean Minimum 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Mean Minimum 18.1 11 24.111 13 21.7 14 23.3 16 22.9 17 16.8 8 8.4 6 Statistic 1 24.1111 24.1111 0.20692 Maximum 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Maximum 23 32 26 28 27 25 13 Acceptable 0.8 - N/A 15 -N/A 15 -N/A 0.13-0.47 SE 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 SE 1.3940 2.2326 1.461 1.2207 1.0899 1.6384 0.7630 Duration Species: Source: Client: Project: MSDp N/A 20.69% 95% UCL 59.15799 89.36781 Range SD 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 0.00000 SD 4.4083 6.6978 4.6200 3.8601 3.4464 5.1812 2.4129 Nautilus Environmental (CA) : 6d 22h Ceriodaphnia dubia In-House Culture Dudek & Associates Method Fisher's Exact Bonferroni Adj t Method Linear Interpolation Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria CV 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% cv 24.36% 27.78% 21.29% 16.57% 15.05% 30.84% 28.73% 000-089-125-1 CETIS™ v1.0256 Analyst:Approval: o CETIS Test Summary Page 2 of 2 Report Date: 03 Apr-07 11.22 AM Link: 08-4701-8123/0702-S052C 7d Proportion Survived Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Detail Control Type Conductivity C Lab Control Rep1 1 .00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep1 15 13 14 19 17 13 12 Rep 2 1 .00000 1.00000 1 .00000 1 .00000 1.00000 1 .00000 1.00000 Rep 2 21 24 14 16 17 12 8 Rep 3 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 3 11 28 20 26 27 22 8 Rep 4 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 4 23 31 24 26 25 25 13 Rep 5 1 .00000 N/A 1.00000 1.00000 1 .00000 1.00000 1 .00000 Rep 5 19 N/A 23 26 24 19 6 Rep 6 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 6 21 32 24 21 23 8 6 Rep 7 1.00000 1 .00000 1.00000 1.00000 1 .00000 1 .00000 1 .00000 Rep 7 14 24 26 24 25 20 8 Rep 8 1 .00000 1.00000 1 .00000 1.00000 1 .00000 1 .00000 1 .00000 Rep 8 22 24 26 21 22 18 8 Rep 9 1 .00000 1 .00000 1 .00000 1.00000 1 .00000 1 .00000 1 .00000 Rep 9 13 27 26 28 23 18 6 Rep 10 1.00000 1.00000 1.00000 1.00000 1 .00000 1 .00000 1.00000 Rep 10 22 14 20 26 26 13 9 000-089-125-1 CETIS ™v1.0258 Analyst:.Approval Qo • •• • • • • Comparisons: Page 1 of 1 -——.^ . i • n t '1 Report Date: 03 Apr-07 1 1 :22 AM L/C I lO AnalySIS U8tail Analysis: 09-0754-8491/0702-S052C Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint 7d Proportion Survived Method Fisher's Exact Group Comparisons Control vs Conc-% Lab Control 6.25 12.5 25 50 100 Data Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Graphics l.On O O 0.9: 1 0.7: •I 0.6- g "1 0.3: 0.2: 0.1- 0 6.25 Analysis Type Sample Link Control Link Date Analyzed Version Comparison 08-4701-8123 08-4701-8123 03 Apr-07 11:16 AM CETISv1.025 Alt H Data Transform II NOEL LOEL Toxic Units ChV MSDp C>T Untransformed 1 100 >100 1.00 N/A Statistic Critical Decision(O.OS) 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1 .00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect Non-Responders Respondent Total Observed 9 09 10 0 10 10 0 10 10 0 10 10 0 10 10 0 10 O O O O 12.5 25 50 100 Conc-% 000-089-125-1 CETIS™ v1.0258 Analyst:Approval:, O CETIS Analysis Detail Comparisons: Report Date: Analysis: Page 1 of 1 03Apr-0711:22AM 12-8266-2273/0702-S052C Ceriodaphnia 7-d Survival and Reproduction Test Endpoint Reproduction Method Bonferroni Adj t Nautilus Environmental (CA) Analysis Type Sample Link Control Link Date Analyzed Version Comparison 08-4701-8123 08-4701-8123 03 Apr-07 11:16 AM CETISv1.025 Alt H Data Transform Z II NOEL C > T Untransformed 25 ANOVA Assumptions Attribute Test Statistic Critical P Level Variances Bartlett 9.60811 15.08628 0.08713 Distribution Kolmogorov-Smirnov D 0.12419 0.13416 0.02413 LOEL Toxic Units ChV MSDp 50 4.00 35.355 20.69% Decision(O.OI) Equal Variances Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Decision(O.OS) Between 1804.655 360.931 5 17.61 0.00000 Significant Effect Error 1085.989 20.49036 53 Total 2890.64404 381.42139 58 Group Comparisons Control vs Conc-% Statistic Critical P Level MSD Decislon(O.OS) Lab Control 6.25 1.15928 2.39879 0.1258 4.98910 Non-Significant Effect 12.5 0.38999 2.39879 0.3491 4.98910 Non-Significant Effect 25 0.58231 2.39879 0.2814 4.98910 Non-Significant Effect 50 3.51523 2.39879 0.0005 4.98910 Significant Effect 100 7.55399 2.39879 0.0000 4.98910 Significant Effect Data Summary Original Data Conc-% Control Type Count Mean Minimum Maximum SD Transformed Data Mean Minimum Maximum SD 0 Lab Control 9 24.111 13 32 6.6978 6.25 10 21.7 14 26 4.6200 12.5 10 23.3 16 28 3.8601 25 10 22.9 17 27 3.4464 50 10 16.8 8 25 5.1812 100 10 8.4 6 13 2.4129 Graphics 10- $ 30- § 20- 10- 0 K j I } I I] ' J t A Reject Nul. 3 I 1 S^" /Q<f> >• / oO 6.25 12.5 25 50 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Conc-% Ranklts 000-089-125-1 CETIS™ V1.025B Analyst:,Approval:. Q o CETIS Analysis Detail Linear Interpolation: Page 1 of 1 Report Date: 03 Apr-07 11.22 AM Analysis: 09-0768-6815/0702-S052C Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Linear Interpolation 08-4701-8123 08-4701-8123 03 Apr-07 11:17 AM CETISv1.025 Linear Interpolation Options X Transform Y Transform Seed Resamples Expanded CL Method Linear Linear 5795186 200 Yes Two-Point Interpolation Point Estimates % Effect Conc-%95% LCL 95% UCL 25 50 44.50000 78.24074 30.06757 61.55914 59.15799 89.36781 Data Summary Conc-% Control Type Count Mean Calculated Variate Minimum Maximum SE SD 0 6.25 12.5 25 50 100 Lab Control 9 10 10 10 10 10 24.111111 21.7 23.3 22.9 16.8 8.4 13 14 16 17 8 6 32 26 28 27 25 13 1.36719 0.94305 0.78793 0.7035 1.05760 0.49254 6.69784 4.62000 3.86005 3.44642 5.18116 2.41293 Graphics 000-089-125-1 CETIS™ v1.0258 Analyst:.Approval: Q o o CETIS Test Summary tfs Page 1 of 1 Report Date: 03 Apr-07 11:55 AM Link: 08-4701-8123/0702-S052C Ceriodaphnia 7-d Survival and Reproduction Test ("?/5*° >*#/£>- } Test No: 09-3605-9635 Start Date: 27 Feb-07 03:25 PM Ending Date: 06 Mar-07 02:00 PM Setup Date: 27 Feb-07 03:25 PM Sample No: 07-2146-8492 Sample Date: 26 Feb-07 02:45 PM Receive Date: 27 Feb-07 01 :30 PM Sample Age: 25h Comparison Summary Analysis Endpoint 13-8686-7570 Reproduction Test Acceptability Analysis Endpoint 13-8686-7570 Reproduction 13-8686-7570 Reproduction 1 3-8686-7570 Reproduction Reproduction Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Detail Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-01 3 (2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Code: Source: Station: Well Water 07-0136 Dudek & Associates WelM NOEL LOEL <0 0 Attribute ChV N/A Duration: Species: Source: Client: Project: MSDp 18.58% Nautilus Environmental (CA) 6d 22h Ceriodaphnia dubia In-House Culture Dudek & Associates Method Equal Variance t Statistic Acceptable Range Control Response 24. 1 1 1 1 Control Response 18.1 MSDp Reps 10 10 10 10 10 10 10 Rep1 15 13 14 19 17 13 12 Mean 18.1 24.111 21.7 23.3 22.9 16.8 8.4 Rep 2 21 24 14 16 17 12 8 Minimum 11 13 14 16 17 8 6 Rep 3 11 28 20 26 27 22 8 0.18576 15 -N/A 15 -N/A 0.13-0.47 Maximum SE 23 32 26 28 27 25 13 Rep 4 23 31 24 26 25 25 13 1.3940 2.2326 1.461 1.2207 1.0899 1.6384 0.7630 Rep 5 19 N/A 23 26 24 19 6 SD 4.4083 6.6978 4.6200 3.8601 3.4464 5.1812 2.4129 Rep 6 21 32 24 21 23 8 6 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria cv 24.36% 27.78% 21.29% 16.57% 15.05% 30.84% 28.73% Rep 7 Rep 8 14 24 26 24 25 20 8 22 24 26 21 22 18 8 Rep 9 13 27 26 28 23 18 6 Rep 10 22 14 20 26 26 13 9 0 o 000-089-125-1 CETIS ™v1.0258 Analyst:.Approval:. Q O • • • • • • *o•••• ••••••• •••••••• CETIS Analysis Detail Ceriodaphnia 7-d Survival and Reproduction Test Comparisons: Page 1 of 1 Report Date: 03 Apr-07 1 1 :55 AM Analysis: 13-8686-7570/0702-S052c Nautilus Environmental (CA) Endpolnt Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 08-4701-8123 08-4701-8123 03 Apr-07 11:18 AM CETISv1.025 Method Alt H Data Transform Z Equal Variance t C > T Untransformed ANOVA Assumptions Attribute Test Variances Variance Ratio Distribution Shapiro-Wilk W ANOVA Table Source Sum of Squares Between 171.1585 Error 533.7889 Total 704.947357 Group Comparisons Control vs Control Lab Control Conductivity Co Data Summary Conc-% Control Type Count 0 Conductivity C 10 0 Lab Control 9 Graphics 40- % 30~ I- ) 10- Statistic Critical 2.30846 6.69330 0.93269 0.86288 INOEL LOEL Toxic Units ChV MSDp <0 0 N/A 18.58% P Level Decision(O.Ot) 0.23446 Equal Variances 0.19363 Normal Distribution Mean Square DF F Statistic P Level Decision(O.OS) 171.1585 1 5.45 31.39935 17 202.55783 18 Statistic Critical P Level 2.33474 1.73961 0.0160 Original Data 0.03208 Significant Effect MSD Decision(O.OS) 4.47885 Significant Effect Transformed Data Mean Minimum Maximum SD Mean Minimum Maximum SD 18.1 11 23 24.111 13 32 i Reject Null 0 0 ££. Conc-% |_C 4.4083 6.6978 10-j ., .S 0 _ S- /OD oeroH 1 ooo/'^ ./0 -10- j/ Q•o -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Ranklts 000-089-125-1 CETIS™ v1.0258 Analyst:Approval:_ o o Freshwater Chronic Bioassay Client/Sample Test Number: Dudek & Assc./ Well-1 Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 2/27/2007 End Date/Time: •?>. t^. <-T7 Cone. Lab*t Control Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup /3 t3> i3 ^>3 \*)1 3 ID \«H *-/•*yn> s>v 3^ }& ^ ^Hi£^ i^ SW Daily Reproduction/ Survival 1 o o o 0 -0U.P o C? 6 o ov-v 2 O 0 oo •e*- 0 0 o 0 0«i 3 O<oo Cj —ooooo £t- 4,o5^-* - U> •5 ^v- fo 4 « KA § . V^ 5-i> to O - <£> a 0 O la^Cj SA !," fr""' ^<Q i "a i*& — \3- 1 1C? l\ (O V^b 7<D n \i H-- 1«A \o •^ tPO M.<— 8 - Total n IX T% 3/ urf ^^' t^( ^f -z-T /f QC h.*,ti Cone. Cond. ' Control ^Itfo) Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 o o o o o o o 0 o o 2 0 0o 0 0 0 0 o o D 3 c^> <j r> 0 & OnOo D "4;v3 "5 C! •5 *=5 ^*> 2> U3 ft , '*' S C3 'V <r\ o c> 3 o 0 O ••; e'r 0 i^-) ft %1 v o( 1It ' 7, > <» 1° O fo <^•^ o\°oq 8 Total /r rz/l H 7,3 /f o-\ '1-zi14. •2-^ QC ro ^»f Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 o O o O o o t)o oo 2 o o o t> o a O o o C? 3 £> O O a 0 o O O P D '4 , Oo -3 <s(oV V V <v^ '"5 Q? •3 o o o o Q O o CD . 6"' % <\ <y\ il » i<? iO fo I'D vo , 7 ' C> O <zf t> f^ ,flf ttTx \1- 0 It- yO •© 8 Total Jf /«Y 7^ iy T-1^2i•Ufu» *L(» -L4> QC fo 12^ Fed (day): (0)(1) I&PD (2) iooc> (3) i (7) — (8) _ ^_ Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: I QC Check:Final Review:_- Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. oo Freshwater Chronic Bioassay Client/Sample ID: Test No: Oudek & Assc./ Well-1 Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna _ Start Date/Time: 2/27/2007 End Date/Time: *,.(«. o"1 Cone. 12.5% Analyst Rep 1 2 3 4 5 6 7 8 9 10 fcu Daily Reproduction/ Survival 1 o o O O CS o 0 O O o 'XM 2 C3 L> e> c. 0 f t?o o (.-> M 3 o Ci nof> r?ooQ o tL ' 4' O (p (jy ^5- S••=5 V.u 5Cp Rj ' 5 >' -?o o 0 O O £> 0 O & **^ & \9^<o II to•, | jO (£7 *l I CJ & Y-L. 7c> O j(5 "\j| iOu?•=5J •& '< 3 $ \> toX 8 Total /^llpl^j. 2-<rfafXidi ^3-\ 1H '2A IX QC ^ wv\ Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1o o O O o ,,-, o ,0o £3 2 o o 0 o or, n o O n 3 /^ £>no^> 0c- OC O 4''1 O 0 G?Cj$ Crj^^7- <_/ ^'r?L "5" 1 In O O 0 O £) 0 On 6 i3 1 1i an I! iO u>rt Vr> "7 ' o o <6 °\ ro df *£>^sif "%• Of<% o> \o $> \ \ 8 Total nn•2. -7 2-S- ln Z"JI'tjKrzs' •2.7. "Z-"3 •£4 QC 1 O»^v) Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 /o(^ nOoo 0 oc> o 2 O &o, o Cs p, Cs f> O O 3 iJ iD ^)O O f) C 0(•} O 4 •• o Q tfO7 U O ^^.5- Q O ,' .5 4iV n o Cs oo 0 «5*S 6 ^% 7io P-, ^tj «j 0 (?) 7 o o\ \is ^•^ o[/) /^f? oo 8 Total 1 "3 1 "Z, "2<T- 2<T/g % •i4>/ *•& IS QC "-^ V-^ Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 O oo 15 O,-, o o„ o 2^ o o o ^ ^<^ o ot> \ 3 Oo0o t) Oo £> d> 0 4o <0 o Q 2_ O 3, O oo 5 "\ 3,i i-^ 0 I ir> \ a 0 6 5 0> <n a J- S 5 "1tp 1 7,', U> **\/n £> '6 n £) O O Ooo 8 Total iiry f/-3 C? frpr fLg ^H? *V QC O P^J (2) itn^O (3)\&5 (7) - (8) - otes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) 0QC Check:Final Review: Environmental, LLC.5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client } Samp 1 ^ TestN t :) O n it * m « • • • • • • • • • • • • • • •^ Analyst •Comme Animal QCChe Nautilus elD: o: Concentration Day Dudek & Associates Well-1 ^Z-roffXo Test Species: Start Date/Time: End Date/Time: C. dub/a 2/27/2007 V6?5 3-b-O"^ IM.C'O Lab Control * I 0 1 2 3 4 567 ">" Initial pH DO (mg/L) Cond. (pmhos/cm) Temp (°C) ty.\° 3.2 3C?o PH. tj <?. ^H ^ \ lI'-V T^5.^ ^.-^T -7."2> l ^i3 35.0 «,2fl ^^IV* iS A S.n cS C) \C?\ t~^ ~^ «;^ ^,,33, LD-~? 'S ) d3^1 S-3o "7, c-) 3H O ^H.5 "•i .^Q • • Final . PH DO (mg/L) Temp (°C)* <fe,OCT ^^^^L»O *™ j « V. O< 2J6 .^ 6.0^ i 0.1 2^.o ^d\ ^.-^ 7-5.| "7.^3, ~)<^ c^HA %c3 &0 ^S"^ "7-^ r ?>.C? 96.0 CS O^P* %% 2\ (J ^fj Concentration Day Conductivity Control ^ ) (^306) 01234567 • ' '-' /.Initial" ' '• "' •' """• ' • "•'•":- •••'.'•• PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) ~l.t\<H <&.*> 2,^0 2U.O ^."i-l 1TA 7"bW> 2-S-^ Cg.^>\ T."V ^JUO pJ& 1 <.u tS-b 3920 2sn Sill _Ei35^0 £^5.") ^, cfl Ul 334 b cQS-H <g) \<^ 7.1 'ja-^p SH.^ " . ' ' •'" .- ' ' ' Final .. - : ,. ••-•...• pH DO (mg/L) Temp (°C) t^-O *-3 2.-S.-3> "7-^ ^.3. ^6-L> 7.^H £^ 'i-SA 0 c5 %"-(OiJ- Q l°n&o •2S-3 "7.?>^ ^.o &<&. o 7,%^ 3.® s^.'S* Concentration Day 6.25% 0 1 2 3 4 5 6 7 Initial pH DO (mg/L) Cond. (Mtnhos/cm) Temp (°C) "7. 6>^3 g> a Mi? s-5. 3. 7- £A <^.7_ HI 7 2-4-0 -q.-^-^ 9s - ^ H"V^ >H-^ l.SM ^,^ uio ^H.-sr ^.05 sg> .3 >\^*5 &H.Q -^-g5<$j ^- O MM^ ^-^••^ ^c|q XI. "^ MM a 9H<5, ' : - Final • ' . ' '.- .' : >:'- pH DO (mg/L) Temp (°C) s: Initial: Final: nts: Source/Date Received: ck: /^, 1-1X- 8-0^ a- ( -i,-s-'^> ^.W S. o 35 o •7.^0 ^M "2^.1 ~^^^ -3 -°\ 3-M-^ ^ \*\ 5-7,0, ^^5;^ ^,.o^V @>.\ a6- P 715 6.f\ ^N-'S' 0 1 2 3 4 5 6-7 tL /wcr R-J> ^^ ^L- st\ A^T ^-^ -JZ-^ 1^^ •=5^ \5_^ T^^^C *n Internal N/A Animal Age at Initiation: Final Review: ^8K-S <£ ^^,. Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. •* Freshi ^Client: ^ Sampl< ,,» TestNc a a o o Q * *• • • • • • • • • • • • • • • • • • • •0 Analysts •^P Commen ^ Animal S •QC Chec _ Nautilus E * /vater Chronic Bioassay Dudek & Associates }ID: r. Concentration Day Well-1 en<n--#>r*~<- Water Quality Measurements Test Species: C. dubia Start Date/Time: End Date/Time: 2/27/2007 I ^>«^3 %-tp-O-T )MoO 12.5% 0 1 2 34567 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) *-} .<-i^ 8. 0 0^1 ^>&.le ~1H5T « J2 14, fc> -•xMr.O *T- -so•& . o (j,0^0! &M..V-? i&a SA TCH 2.1.S rj.vaft "2j. ^> V£)g)U ^•5 , V ~3 ~(<yQ ^ A (a ~^-^ ->^>^ "1-"=}- \ ~^V -I T- oo S«-)-T : •-": Final /'•". :••",."•:• : : -, • PH DO (mg/L) Temp (°C) ^.0 ^ 9, . v.>-« ,=-, g.00> & • ( 9 $-0 1ft> •ft. 4 I-**. \ "^-OfS «- 0 ^4 .^ ^.tS "&' 1 ^15 ^ ^-°\?> •Qi-'T— 5e>.cp 7.^7 8-cS ^wf, ^ Concentration Day 25% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) •7-23 €>. o (o^f "7 3&.'-l 7, l-i 7-.*\ \~l~11 •I2r^~" V ^V *&• Q \\°l(J5 ^M.5 n-H\ «.•« IV2S iH/3 IV, -5 \ •Ss . ^ l-2.-Z-^) ^•5> . O "^MT- ^>- v \1'2.2. ^^^ "^M") -4 t> \ v ^>3 5M-^> -z>V.o Final PH DO (mg/L) Temp (°C) -^-cm ©.v ><3 -*i •?. T1 & -d- 35.0 ")-*7 <Hjr Z-£f -^-°l 3 S "^ ^vV.°\ "7. *?(-$> ^O £5 2> 3-^T- <& . * 0-6 -0 "7. *? o £>. ~? D.M. 2) Concentration Day 50% 0 1 2 3 4 5 6 7 ' • • " ' .•••• , •./'-'•• ••'Initial..'' : ' '- •' "" :'••'•' • :« .':'.' ; ; ' PH DO (mg/L) Cond. (pmhos/cm) Temp fC) t/ SV-?. f, U&58> P-6.3 (v^ 1.1 *^.\l\ V-* -° u-°i 5 "g-O 307.0 ^H o n.n •3,3 V^O*r-V'i •^ . T-£) O ^-> V? * \°-)£)Cj •>M.I *? 'S ^». ? 1^1-5 ors 8 •TL ^> "*- tJ- \ (^ CaZ SM-O5 •' • , " .'.'•: • ••"'.- Final • ••-'•' . . '" . .•:••' PH DO (mg/L) Temp (°C) ^•S~¥ ^5- T_ 3-5 , ° n 13 &3 25. 0 l.tl %• ? 2_^ 1 "=}• ^54 <& - ""— 5M-^ "?-.<'? O s-o --^-.3 •%. e>i^ ft- 2- 9^5. O 7.<2>(B>ft.'a -2-H.ft Concentration Day 100% 01234567 Initial PH DO (mg/L) Cond. (|jmhos/cm) Temp (°C) Ce-^5> K^r". d^V 3M^O s^ 3 (j-'f/! 7 1 ^Mlo -2_H--0 ^(o 4 ~3 1-. ^ 3M oo ^•O C.5-S- •4.^ 3-54.0 Z-H.V to • ^3 " 2>. u ^^o >H,«-f ^ Q 5 ^.M. 3>H?-0 &-5"?i Co-^T Q ^35.TO JM-O .• Final PH DO (mg/L) Temp (°C) Initial: Final: ts: ource/Date Received: k: fa, ^'TX'* ~3f3-^ ft-^3 ^-5 .^> 1-Sl %.-5 ^6.0 •7 <2 ^.<r "2-5". 1 •^.^.c( 8.\ ^H .*! •^,^4-<A\ j5fsp, "T- ,"=5 \ (^ .ti 3^.0 T. '((a 8 -~1 Ck-M-'^, 01234567 ^<-"^1 ^-i Us b I/ 1L*^ L->^ sw A^V o *\ '12^. L v fc.5 Sb R. ^ ^A , • " '."' ".:'' ^•*- 1 Internal N/A Animal Age at Initiation: Final Review: nvlronmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. ^8K,5 ^^ oo Site: Well #3 CETIS Test Summary Page 1 of 2 Report Date: 03 Apr-07 11:45 AM Link: 15-2200-2891/0702-S052d Ceriodaphnla 7-d Survival and Reproduction Test Test No: 05-8250-6996 Start Date: 27 Feb-07 02:45 PM Ending Date: 06 Mar-07 09: 1 5 AM Setup Date: 27 Feb-07 02:45 PM Sample No: 1 8-5490-071 7 Sample Date: 26 Feb-07 01 :35 PM Receive Date: 27 Feb-07 01 :30 PM Sample Age: 25h Comparison Summary Analysis Endpoint Test Type: Reproduction-Survival (7d) Protocol: EPA/82 1/R-02-01 3 (2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Code: Source: Station: Well Water 07-0137 Dudek & Associates Well 3 NOEL LOEL 07-5847-3316 7d Proportion Survived 50 05-6970-4056 Reproduction 25 Point Estimate Summary Analysis Endpoint 100 50 % Effect Conc-% 01-9820-4927 7d Proportion Survived 50 06-4023-4168 Reproduction Test Acceptability Analysis Endpoint 25 50 87.05505 37.95787 55.95878 Attribute ChV 70.711 35.355 95% LCL 61.49250 29.75694 45.68713 Nautilus Environmental (CA) Duration: 6d 18h Species: Ceriodaphnia dubia Source: In-House Culture Client: Project: MSDp N/A 21.37% 95% UCL 123.24400 43.03161 62.85225 Statistic Acceptable Range 01-9820-4927 7d Proportion Survived Control Response 1 0.8 -N/A 07-5847-3316 7d Proportion Survived Control Response 1 0.8 -N/A 05-6970-4056 Reproduction Control Response 23.4444 15 -N/A 06-4023-4168 Reproduction Control Response 23.4444 15 -N/A 05-6970-4056 Reproduction 7d Proportion Survived Summary Conc-% Control Type Reps 0 foo 0 6.25 12.5 25 50 100 »o^ Conductivity C 10 Lab Control 10 10 10 10 10 10 Reproduction Summary Conc-% Control Type Reps 0 (f)0 0 6.25 12.5 25 50 100 **} Conductivity C 10 Lab Control 10 10 10 10 10 10 MSDp Mean 0.80000 1 .00000 1.00000 1.00000 1.00000 0.90000 0.40000 Mean 2.2 23.444 22.5 23.1 22.3 13.2 0.8 0.21368 0.13-0.47 Minimum Maximum SE 0.00000 1.00000 0.13333 1.00000 1.00000 0.00000 1.00000 1.00000 0.00000 1.00000 1.00000 0.00000 1.00000 1.00000 0.00000 0.00000 1.00000 0.10000 0.00000 1.00000 0.16330 Minimum Maximum SE 0 14 13 17 14 3 0 6 29 29 31 28 18 7 0.6960 1.6082 1.8089 1.5308 1.461 1.3646 0.6960 SD 0.42164 0.00000 0.00000 0.00000 0.00000 0.31623 0.51640 SD 2.2010 4.8247 5.7203 4.8408 4.6200 4.3153 2.2010 Dudek & Associates Method Fisher's Exact Bonferroni Adj Wilcoxon Rank Sum Method Trimmed Spearman-Karber Linear Interpolation Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria cv 52.70% 0.00% 0.00% 0.00% 0.00% 35.14% 129.10 CV 100.05 20.58% 25.42% 20.96% 20.72% 32.69% 275.13 000-089-125-1 CETIS™ v1.0258 Analyst:Approval: ? O CETIS Test Summary Page 2 of 2 Report Date: 03 Apr-07 11:45 AM Link: 15-2200-2891/0702-S052d 7d Proportion Survived Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Detail Control Type Conductivity C Lab Control Rep1 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep1 4 14 13 18 17 15 0 Rep 2 0.00000 1.00000 1.00000 1.00000 1.00000 1 .00000 1.00000 Rep 2 0 29 14 17 14 18 7 Rep 3 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 3 3 24 22 27 24 12 1 Rep 4 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 4 0 25 28 28 28 15 0 Rep 5 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 5 1 27 27 21 23 18 0 Rep 6 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 6 1 24 26 24 25 11 0 Rep 7 1.00000 1 .00000 1.00000 1.00000 1 .00000 0.00000 1.00000 Rep 7 6 26 29 23 17 3 0 Rep 8 1.00000 N/A 1.00000 1 .00000 1.00000 1.00000 0.00000 Rep 8 5 N/A 25 17 25 15 0 Rep 9 1.00000 1.00000 1.00000 1 .00000 1.00000 1.00000 0.00000 Rep 9 2 25 23 25 24 12 0 Rep 10 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 10 0 17 18 31 26 13 0 000-089-125-1 CETIS™ v1.0258 Analyst:.Approval: CETIS Analysis Detail Comparisons: Page 1 of 1 Report Date: 03 Apr-07 11:45 AM Analysis: 07-5847-3316/0702-S052d Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version 7d Proportion Survived Comparison 15-2200-2891 15-2200-2891 03 Apr-07 11:43 AM CETISv1.025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Fisher's Exact C>T Untransformed 50 100 2.00 70.711 Group Comparisons Control vs Conc-%Statistic Critical Decision(O.OS) Lab Control 6.25 12.5 25 50 100 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 0.52632 0.05000 Non-Significant Effect 0.00774 0.05000 Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed 0 6.25 12.5 25 50 100 Lab Control 9 10 10 10 9 4 9 10 10 10 10 10 Graphics l.O-i O 0.8- 0.7: O.fr! 0.5: O.^i 0.3-i 0.2: 0.1- 0.0 6.25 12.5 25 Conc-% 50 100 000-089-125-1 CETIS™v1.025B Analyst:Approval:/ ./pis CETIS Analysis Detail Spearman-Karber: Page 1 of 1 Report Date: 03 Apr-07 11:45 AM Analysis: 01-9820-4927/0702-S052d Ceriodaphnia 7-d Survival and Reproduction Test Endpoint Analysis Type 7d Proportion Survived Trimmed Spearman-Karber Spearman-Karber Options Threshold Option Lower Threshold Trim Level Mu Nautilus Environmental (CA) Sample Link Control Link Date Analyzed Version 1 5-2200-2891 1 5-2200-2891 03 Apr-07 1 1 :43 AM CETISvl .025 Sigma Control Threshold 0 40.00% 1.939794 0.07548594 Point Estimates EC50/LCSO 95% LCL 95% UCL 87.05505 61.49250 123.24400 Data Summary Calculated Variate(A/B) Conc-% Control Type Count Mean Minimum 0 Lab Control 9 1.00000 1,00000 6.25 10 1.00000 1.00000 12.5 10 1.00000 1.00000 25 10 1.00000 1.00000 50 10 0.90000 0.00000 100 10 0.40000 0.00000 Graphics 0.9- Q. 0.8- \. 1 0.7- N. * 0.6- \. '•P 0.5: \. | 0.4: TO 5 0.3: 0.2: 0.1: 0 20 40 60 80 100 Conc-% Maximum SE 1.00000 0.00000 1.00000 0.00000 1.00000 0.00000 1.00000 0.00000 1.00000 0.06455 1.00000 0.10541 SD A B 0.00000 9 9 0.00000 10 10 0.00000 10 10 0.00000 10 10 0.31623 9 10 0.51640 4 10 000-089-125-1 CETIS™ v1.0258 Analyst:,Approval:, O CETIS Analysis Detail Comparisons: Page 1 of 1 Report Date: 03 Apr-07 11:45 AM Analysis: 05-6970-4056/0702-S052d Ceriodaphnia 7-d Survival and Reproduction Test Endpoint Reproduction Method Nautilus Environmental (CA) Analysis Type Sample Link Control Link Date Analyzed Version Comparison 15-2200-2891 15-2200-2891 03 Apr-07 1 1 :44 AM CETISv1.025 Alt H Data Transform Z || NOEL Bonferroni Adj W Icoxon Rank Sum C > T Untransformed II 25 ANOVA Assumptions Attribute Test Variances Bartlett Statistic Critical P Level 7.14965 15.08628 0.20975 Distribution Kolmogorov-Smirnov D 0.15577 0.13416 0.00110 ANOVA Table Source Sum of Squares Mean Square DF F Statistic P LOEL Toxic Units ChV MSDp 50 4.00 35.355 21.37% Decision(O.OI) Equal Variances Non-normal Distribution Level Decision(O.OS) Between 4085.722 817.1443 5 39.55 0.00000 Significant Effect Error 1094.922 20.65891 53 Total 5180.64417 837.80326 58 Group Comparisons Control vs Conc-% Statistic Critical P Level Ties Lab Control 6.25 12.5 25 50 100 Data Summary 97 0.4211 6 96.5 0.3901 4 91 0.2483 5 62 0.0005 5 55 0.0000 3 Original Data Conc-% Control Type Count Mean Minimum Maximum SD 0 Lab Control 6.25 12.5 25 50 100 Graphics 40- § 30- J1 20- 10- Decision(O.OS) Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Significant Effect Transformed Data Mean Minimum Maximum SD 9 23.444 14 29 4.8247 10 22.5 13 29 5.7203 10 23.1 17 31 4.8408 10 22.3 14 28 4.6200 10 13.2 3 18 4.3153 10 0.8 0 7 2.2010 i 1 ' I 1 ( -1 1 h -- ®S^ 5: /{&X-Q33 ;S OO >xT °° 0 6.25 12.5 25 50 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Conc-% Ranklts 000-089-125-1 CETIS™ v1.0258 Analyst:,Approval: CETIS Analysis Detail Linear Interpolation: Page 1 of 1 Report Date: 03 Apr-07 11:45 AM Analysis: 06-4023-4168/0702-S052d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Linear Interpolation 15-2200-2891 15-2200-2891 03 Apr-07 11:44 AM CETISv1.025 Linear Interpolation Options X Transform Y Transform Seed Resamples Expanded CL Method Linear Linear 3019480 200 Yes Two-Point Interpolation Point Estimates % Effect Conc-%95% LCL 95% UCL 25 50 37.95787 55.95878 29.75694 45.68713 43.03161 62.85225 Data Summary Conc-% Control Type Count Mean Calculated Variate Minimum Maximum SE SD 0 6.25 12.5 25 50 100 Lab Control 9 10 10 10 10 10 23, 22.5 23.1 22.3 13.2 0.8 14 13 17 14 3 0 29 29 31 28 18 7 0.98484 1.16766 0.98812 4.82470 5.72033 4.8408 0.94305 4.62000 0.88087 4.31535 0.44928 2.20101 Graphics 25-c 15- 20 40 60 Conc-% 100 000-089-125-1 CETIS™v1.025B Analyst:,Approval:. e CETIS Test Summary frk»l Page 1 of 2 Report Date: 03 Apr-07 11:50 AM Link: 15-2200-2891/0702-S052d Ceriodaphnia 7-d Survival and Reproduction Test 5}"°^ M*/Ct* Test No: 05-8250-6996 Start Date: 27 Feb-07 02:45 PM Ending Date: 06 Mar-07 09: 1 5 AM Setup Date: 27 Feb-07 02:45 PM Sample No: 18-5490-0717 Sample Date: 26 Feb-07 01 :35 PM Receive Date: 27 Feb-07 01 :30 PM Sample Age: 25h Comparison Summary Analysis Endpoint 1 8-8932-31 01 7d Proportion Survived 07-3788-6040 Reproduction Test Acceptability Analysis Endpoint 18-8932-3101 7d Proportion Survived 18-8932-3101 7d Proportion Survived 07-3788-6040 Reproduction 07-3788-6040 Reproduction 07-3788-6040 Reproduction 7d Proportion Survived Summary Conc-% Control Type Reps 0 Conductivity C 10 0 Lab Control 10 6.25 10 12.5 10 25 10 50 10 100 10 Reproduction Summary Conc-% Control Type Reps 0 Conductivity C 10 0 Lab Control 10 6.25 10 12.5 10 25 10 50 10 100 10 Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013(2002) Dil Water: Diluted Mineral Water (8:2) Brine: Material: Well Water Code: 07-0137 Source: Dudek & Associates Station: Well 3 NOEL LOEL 0 >0 <0 0 Attribute Control Response Control Response Control Response Control Response MSDp Mean Minimum 0.80000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.90000 0.00000 0.40000 0.00000 Mean Minimum 2.2 0 23.444 14 22.5 13 23.1 17 22.3 14 13.2 3 0.8 0 Statistic 1 0.8 23.4444 2.2 0.12535 ChV N/A N/A Duration Species: Source: Client: Project: MSDp N/A 12.54% Acceptable Range 0.8 - N/A 0.8 - N/A 15 -N/A 15 -N/A 0.13-0.47 Maximum SE 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.13333 0.00000 0.00000 0.00000 0.00000 0.10000 0.16330 Maximum SE 6 29 29 31 28 18 7 0.6960 1.6082 1.8089 1.5308 1.461 1.3646 0.6960 SD 0.42164 0.00000 0.00000 0.00000 0.00000 0.31623 0.51640 SD 2.2010 4.8247 5.7203 4.8408 4.6200 4.3153 2.2010 Nautilus Environmental (CA) : 6d 18h Ceriodaphnia dubia In-House Culture Dudek & Associates Method Fisher's Exact Equal Variance t Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Fails acceptability criteria Fails acceptability criteria cv 52.70% 0.00% 0.00% 0.00% 0.00% 35.14% 129.10 CV 100.05 20.58% 25.42% 20.96% 20.72% 32.69% 275.13 000-089-125-1 CETIS™ v1.0256 Analyst:,Approval:. O CETIS Test Summary Page 2 of 2 Report Date: 03 Apr-07 11:50 AM Link: 15-2200-2891/0702-S052d 7d Proportion Survived Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Rep1 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 2 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 3 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 4 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 5 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 6 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Rep 7 1.00000 1 .00000 1.00000 1.00000 1.00000 0.00000 1.00000 RepS 1 .00000 N/A 1.00000 1.00000 1.00000 1 .00000 0.00000 Rep 9 1.00000 1.00000 1 .00000 1.00000 1 .00000 1.00000 0.00000 Rep 10 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Rep1 4 14 13 18 17 15 0 Rep 2 0 29 14 17 14 18 7 Rep 3 3 24 22 27 24 12 1 Rep 4 0 25 28 28 28 15 0 RepS 1 27 27 21 23 18 0 Rep 6 1 24 26 24 25 11 0 Rep 7 6 26 29 23 17 3 0 RepS 5 N/A 25 17 25 15 0 Rep 9 2 25 23 25 24 12 0 Rep 10 0 17 18 31 26 13 0 000-089-125-1 CETIS ™v1.025B Analyst:_Approval: Q CETIS Analysis Detail (0 Comparisons: Report Date: Analysis: Page 1 of 1 03Apr-0711:50AM 18-8932-3101/0702-S052d Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpolnt Analysis Type Sample Link Control Link Date Analyzed Version 7d Proportion Survived Comparison 15-2200-2891 15-2200-2891 03 Apr-07 11:48 AM CETISv1.025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Fisher's Exact OT Untransformed >0 N/A N/A Group Comparisons Control vs Control Statistic Critical Decislon(O.OS) Lab Control Conductivity Co 0.26316 0.05000 Non-Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed Conductivity Co 8 Lab Control 9 10 9 Graphics i.o- 0.9- 0.8- 0.7- 0.6- 0.5- 0.4- 0.3- 0.2- 0.1- 0.0 0 1C Conc-% 000-089-125-1 CETIS™ V1.025B Analyst:,Approval:, o oo CETIS Analysis Detail u, . Comparisons: Page 1 of 1 Report Date: 03 Apr-07 11:50 AM Analysis: 07-3788-6040/0702-S052d Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 15-2200-2891 15-2200-2891 03 Apr-07 11:48 AM CETISv1.025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Equal Variance t OT Untransformed <0 N/A 12.54% ANOVA Assumptions Attribute Test Statistic Critical P Level Decision(O.OI) Variances Variance Ratio 4.80505 6.69330 0.03051 Equal Variances Distribution Shapiro-Wilk W 0.92241 0.86288 0.12600 Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Declsion(O.OS) Between Error 2137.862 229.8222 2137.862 13.51895 1 17 158.14 0.00000 Significant Effect Total 2367.68428 2151.3810 18 Group Comparisons Control vs Control Statistic Critical P Level MSD Decislon(O.OS) Lab Control Conductivity Co 12.5753 1.73961 0.0000 2.93886 Significant Effect Original DataData Summary Conc-% Control Type Count Mean Minimum Maximum SD Transformed Data Mean Minimum Maximum SD Conductivity C 10 2.20000 0.00000 6.00000 2.20101 Lab Control 9 23.4444 14.0000 29.0000 4.82470 Graphics 3<h 25- S '•§ 20- 15- 10- 0 CC Conc-% 0 LC -6- -8- •10--2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Rankits 000-089-125-1 CETIS™ v1.0258 Analyst:_Approval:. o Freshwater Chronic Bioassay © Client/Sample Test Number: Dudek & Assc./ Well-3 Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pa/ex D. magna Start Date/Time: 2/27/2007 End Date/Time: g>.tg-cl Cone. Lab** Control Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup \1 O 15 \1> \\ ^ ^i~b ^H ^\H\e> P4•v-y 3>fc HH 5* T^> 3U l£U SW Daily Reproduction/ Survival 1 0 O o o 0o 0 0 oo a-i 2 o 0 o o 0 o o o 0 O ,-^^ 3 , o o o O o o o o 0 C? £<- ..,,... . O V Cr 3 3 <& cS ^ ^Vo &A :;;':;$""; <^.. <£> o 0 o 0 / ofcf o 3k $.t> '"ff ' <=\ \3> \ D\(h ((\\ -•l^^w~ r o \i.°( \\ 1U-&°( _ ^o WJL, 8 - Total If -i^\ Tx^fz-r^-~)z>r-L& u>fr Zs*rn QC Qte^ Cone. Cond. ^ Control (r/><?°l Rep 1 2 3 4 5 6 7 8 9 10 Dajly Reproduction/ Survival 1 0 0 O oo o o o<J o 2 o •o O O o O o t~\ o o 3 o o ^ e(t> €? O O O 0p/ .' 4,.,, o 4/o o — o o o 0 o o ' '5... «3k — O - /o 3^ Q n 0 6 0 — O -c> o n *o p 7 1- — 3~ o1 4 6> ^c> 8 — - Total V n fi-/<# ?°/<xI Itr^ ft QC #=, vtc Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 O 0 o o 0 o 0 o o O 2 o o O o o O o C5 0 O 3 O O 0 Oo D O C? o D "4';"v c^ V*><^s &5 ^S^5- V 5 Oo o o oooo a 0 6q*i i> \ 0 lo 413. 9> 1 IH 7 D 0 !<? V2-\1- \1s\\\1-q 0 8 Total /? /•/ 2.1^ •2% 2-7 24 2^ ^T X? tr QC )\10. Time Fed (day): (0)(2) \OftO (3) Hop (4) 1 Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) i Comments: QC Check:Final Review: Nautilus Environmental, LLC. 5550 More/rouse Drive, Su/te 150. San Diego, CA 92121. o o Freshwater Chronic Bioassay Client/Sample ID: No: Dudek & Assc./ Wei I -3 0-702, -.To 5"^ Jl Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna _ Start Date/Time: 2/27/2007 End Date/Time: 2,.^ Cone. 12.5% Analyst Rep 1 2 3 4 5 6 7 8 9 10 V;>- Daily Reproduction/ Survival 1 D O <~> O •o o 0 o o o ^-> 2 o *o r~> o o o O O O O <2-A 3 o O o O u o 0oo o fr_ 4 o 5 5 q- Ljy Sq u <£> •5 ^ 5© D O 0p> o 0o 0o •=*«v ,' 6 >> \2^ H l^ n io *3v 1 ii 13 VJu 7 0 Cuy?•c *•" Lo •8:i^) IULC_ 8 Total /^n ^17>-^\ 2X 1,1 1-7 LS" IV QC / ^)\CA_ (2) vopo (3)(41 /VoV Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 oo o <~>oo o o oo 2o 0 o 0 o o 0 0 o O 3 r> 0 O G> C> Oe> o 0o 4 os5 O1 ^ 05 ^S"a '5' ' O n n r\ o & On 0 C7 6 !> 10 1C?i a- !«. i? ^i\ "\ U 7 C) O9loq1-\to \o 1 8 Total /-? )H %M T^- -2/1-z/rn7^- ^M 7^ QC 'Tt£- Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 -o o o o o oo oo o 2 o o oo o 0o o oo 3o&o•o oo oo oo 4 •C^ Oa,o M M ^M vS ^~ •' -§' ' •5 <4> D M>Ou o Qo O •"•'6 1 01*<\*\& T t/D "77 * 7 o O 0 0q 0.— 4o (9 8 *"""^ Total jrI*J-z.irfr i ,i/J/<- ^>~ir \T> QC 0^<- Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 0 -o o> o 4/c. O O 4.1 O O o 2 o o<=> d/o 4/0 c. o — ^-N o 3 Oo 0 —• — 3/Q £»— ^/P 4 o o o — — - . o . — Q - 5 0 ^L. O— — - 0— t» -~ 6 £? Oc> — ——<p— €> - 7 o 5 1 — — O — old' 8 —- ~~ **' Total o ~l 1 of* e /a 0>^\ 0 (>/<St o/A t> fa QC C>lc:c (7) - (8). : d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) Comments: JQC Check:Final Review: utilus Environ 7^ ironmental, LLC.5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Q Freshwater Chronic Bioassay Water Quality Measurements *«* ClientQ O Samp O Test N C • • •• • • • •• • •• • • •• ••• •• • •» W Analyst • ^^ Comme • ^^ Animal QC Che Nautilus • • • elD: o: Concentration Day Dudek & Associates Well-3 0-701. -sor^Ji Test Species: Start Date/Time: End Date/Time: C. dub/a 2/27/2007 1 ^HS 3 M(?7 to'tt^ Lab Control * "2- 01234567 <0 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) g.oO- &.O [*[(, 36.0 # 'a.'A ^?rO t 4H ^.1 8- *=$ 1 • & (^"4 >-5 ' ° 9,» •s^ WM 2.SA fe-iv % -1 \^ ^ ^^ ,\ %.2-S •^ >'::)- ^ ^ 0"5^ ^.7.^ •^ Ctf SviyO 3^\.^ Final PH DO (mg/L) Temp (°C) "^fz- 'R-V 'to,2? 8.16 €>- \ 3-5.0 D>^ ^"^*2_<,l <& .{-\- -A .°\ SM.°I ?r. 9^b ^:O 953 "-*• 8"^ 6M ^5.0 "7-^*7 8 -<^ 3H€> Concentration Day Conductivity Control *Z (sooo") 0 1 23456 7 Initial pH DO (mg/L) Cond. (jjmhos/cm) Temp (°C) <2>.co -7.1 '-I^Go •2M.^ Sf-K ?-•) So^0 "^-H-.^ e> -"2-0 %>-\ ^t5(0 >l-5 •4-lfc 4/S 5000 i%.^ S.08s> , •% ^'ho.o 5-^^ . o 8.o«=» &.o S>31 O ="5 ^> Sty •^.T ^vj> •& 0 a^v. ^ • . ;:' ' "v '••'•' '" Final ' ' •' "- ' •..'•'• •.'•'. .• :'-. PH DO (mg/L) Temp ('C) "^ri3 ft -v •2-0- "b 7.^1 &.tfl 3Z-0 1,^7 «-, ^ "2-C 1 ^<^<^Sb-7. S^H.CI 'Bvc^V 0^.0 ^^^ -^-S^ §.3 3^G> 7.gCo ^ (j, ^t/.-S Concentration Day 6.25% 0 1 2 34567 ' ' < Initial pH DO (mg/L) Cond. (pmhos/cm) Temp ('C) ~/.-7<y G.^ £6>S £•5. 7 7.^4 1?-;, Z~4 1- T '-^^ ^>. ^ {o\ ~}~ >H.Lff n^l «•! ^•\« ZH.^ B-os i.^> 5"% 5> ">^.e*) 8 .\^ ^».^ Co\vo 9 H.o <& .C5-- <\- (d \J>~*'~2- S-H-^ Final <r^ f |O^ pH DO (mg/L) Temp (°C) s: Initial: Final: nts: Source/Date Received: ck: /^3-7jf ^> -o -«-( tS . t- 2--5.-S €>. \<y Q. I ^5.0 ^-QS ^ M 2-S. ( 9>-Cfe g.. O oA.0! '8^ o-Oass 8-O^ «.<s> a'o-o &.o° 6 -] Q.M_Sfe 0 1 2 3 4 S 6 7 t<-•^jr p-J fu^ VL, S1V ^a ^^ ?~^ ^-^ "?=^ fc_* v^^^u *^S r-~<3~~) Internal N/A Animal Age at Initiation: Final Review: z-bv,^^ ^ «/-«/- Environmental, IXC. 5550 Aforeftouse Drive, Suite 150. San Diego, CA 92121. o Freshwater Chronic Bioassay Water Quality Measurements Q Client: 0 Sample (Q Test N< 0 o 00 ^^^B ^^s0 •••••• • • •• •• *• •••» • •• Analysts • Commen A Animal S 0 QC Chec 0 Nautilus E • >ID: 3: Concentration Day Dudek & Associates Well-3 en en, -rss-^ Test Species: Start Date/Time: End Date/Time: C. dud/a 2/27/2007 iMM^S }/o>|A-7 o^^ ' ' 12.5% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp ("O ~7 -6^ -7. -7 ft^5 3(0. O PH DO (mg/L) Temp (°C) "7.. dO <£, i <^7•1x^.1 "?> .O<0 « • \ 3.f5 <•?> -q- "5 "i- S-o lo \ 5 ^M.l 1, SI S.-7 'Ifel 2.H3 "V^^<e> . ^ °IOOP^ ."? ~^ &^ -V-T. @^^ 3-^-0 T-T-M ~=V. *?c ^^^3*4. ^> Final £. O 6- 1 2!?. o J'-O^<g(>i "2-S-A 'S'OS €> • v 'iH'"3' < TKOtf ^T.^ ^5.S fe-o-5 €- 2_ ^^ -C> &.o > g> ^"7 JX/.^ Concentration Day 25% 0 1 2 3 4 5 6 7 Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) "7-3>( "7- "^ txS<> 25.% 7. 3f, <S.f> \ 6^3 ~LLir.~l "^ -3^. ~V-^rv^^o 3-^-^ 1 21 «.T ITM^ Z.H-3 •> u> ^ <Sg> . M. (5-%- 0> 3-M.""1 "^l A\ v^.£> ^o2- a'-X-U' 1- -M^T•^ g> 1^)5 SMM Final PH DO (mg/L) Temp (°C) Concentration Day "S"lH^>- 1- 2--$ .=?> ^ .1 <-» %.? a6 .0 ^-'6 & f> T-<J ft-V"^ &• V ^-A."5! ^. )o %"D o^.-^ B> \ ^ fe ^^•=5.0 8. o.5 6- 8 £it .c2, 50% 0 1 2 3 4 5 6 7 /Initial PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) "7-0 <§> "7.5ai^yo 35. <=i •7AO 1.4 "ic^lO V-\ vft 'l-.'OVo ~V-Q 30 >tiOM . v T.OS^ •s.<\ 3010 ZH,Z "3 ,^3> <& • ^ ^i^^lO -S-M..-^ rk \^ q ,\ ^•55^ i>6 .O -=)^O *& • \ ^"^7. O a->4. -z- Final •$0)^.3:3 PH DO (mg/L) Temp (°C) €>a^ P>-7/ X-5 .^> fe.3 1 «- i J2-5.0 ^. -^t it A 2£ .\ ft .X3i S.O >A«^ ^-4^: &o 5<g^ S-zi- €>-S 9-5. o 'ft-H ^'13^4 -g, Concentration Day 100% 0123456 7 Initial PH DO (mg/L) Cond. (^mhos/cm) Temp (°C) G>-S^ *?* b.C>•&CQO ^Itf- O 6 . \ M'6-^ ^J5 1 ^ IxV.o ^-ft0! ^-•5 5o 1 0 >"5."^ t.«* 1J.3 H^tD XH.D "^ ^7, •S. ^ <5O -^C dH-1-' '=\-Q^ "^- \ VJ^'h^O ^ \-A O -^ . Q ^ e> .T M*/ "6 O >M. 1 Final PH DO (mg/L) Temp (°C) Initial: Final: ts: ource/Date Received: k: fit- "5-* 7^"' fe-'-t S) ^.UV ^^.'S &M0 &d. 3t>.& •^.T-l •3.^ 7J>-/ ^-^T? R. ff. 1 LP "^-o «^^^ S -v^H 'S-'v} ^•5,0 B- t?~T S.-7 ^?W.^> 01234567 I**"v-iT pL_^ P-i ILU £\A r>M:^>"P_> 12_A "^Tx R-3 >R,^PL Internal N/A Animal Age at Initiation: Final Review: i.t?h/!> /<- nvironmental, LLC, 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. o o o o o o o o Appendix B Sample Receipt Information o ooooooooo o o c o 0 Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Client: J^LoJI^ *c frfS*tJ**#j Sample ID: C0S~\S\&j)t £*lf £*W£-€_- Sample (A, B, C): Log-in No. (07-xxxx): Sample Collection Date & Time: Sample Receipt Date & Time: Check-in Temperature (°C) Temperature OK? DO (mg/L) pH (units) Conductivity (uS/cm) Salinity (ppt) Alkalinity (mg/L)* Hardness (mg/L)*1 Total Chlorine (mg/L) Technician Initials tWr^i/h 0*7- 0\3^ 2/24(^7 /*/0c> 24-7/,7 /33o 7-ff* Y N /&$ ~7.(oQ l^d f.tj 1XT °l(Jt O^-L, /h^ Tests Performed: TestlDNo(s).: ^OtxJA5-fW^ -0135* 7^2^/7 l3^o Cw "t.l Y N /<•-£" 7^o 2X?« M -zx>r 6?"4 />.i>\ ^i^ U«J( 1 -C>I36 2/2^/07 yWT ^ /^Z- Y N r.r b.n ?Vro /.7 T7 13fc o^"2- /** Qefibvpfw ?«^ Oics-^os"i- uM S -6J31 Z^/7 /7/r 8.T Y N $"-2_ ^,"73 rar» 2^& 1SKt> /5-7H ^.07 /nr = mg/L as CaCO,,' = Measured for freshwater samples only, NA = Not Applicable iFreshwater Testsil s~i Control/Dilution Water Source: (8:2; Culligan Additional Control? /?) N = Marine Tests: Additional Control? Sample Salted w/ artificial salt? Sample salted w/brine? Other Alkalinity:__£Z_ Hardness:. ^Z. Hardness: 27^ LAB SW ART SW Other Y N = Y N If yes. what Dot? Y N If yes, what not? Alkalinity: Alkalinity: Salinity: Salinity: Sample Check-In Information Sample Description: 4 1 -f 3 : COC Complete? ABC Filtration? Y ft) Pore Size: Organisms or Aeration? Y _ fi) Length of Time: Final DO: Final pH: pH Adjustment? Y Initial pH: Final pH: Debris CI2 Adjustment? Y /fi> A B Initial Free CI2: STS added: Final Free CI2: Sub-samples for additional chemistry: Comments: Temperature for sample must be 0-6"C if received >24 hours past collection time. QC Check: . Final Review: o o o a o o a o a 0a Appendix C Chain-of-Custody Form M**V]iJ/NAMtilu5 c>mMro •••••••••••••••00o ^CALIFORNIA D WASHINGTON D BRITISH COLUMBIA ' 5550Morer0BeDme.SuSe150 5009 Pao&Hghway&Et.Suite 2 8664 Commerce Court San Kego, Coforia 92121 TbmVteriiglon 98424 Bumaby, British Columbia, Canada V5A4N3 Phone 858.587.7333 Phone 253.922.4296 Phone 604.420.8773 Fax 858.587.3961 Fox 253.922.5814 Fax 604.357.1361 a a o o o o © o • © ® © o Chain of Custody Invoice to: Company Address City RECEIVED BY (COURIER) i AddHional costs may be required for sample disposal or storage. Net 30 unless otherwise contracted.DISTRIBUTION: WHITE - Nautilus Environmental, COLOR - Originator DUDEK 605 THIRD STREET ENCINITAS, CALIFORNIA 92024 T 760.942.5147 F 760.632.8710 L.ETTER OF TRANSMITTAL DATE: Apr 09, 2007 JOB NO. 4821-02 RE: Carlsbad Municipal Golf Course Water Quality Testing To:Dudek 1645 S. Rancho Santa Fe Road Suite 202 San Marcos, California 92078 Attention: George Litzinger We are sending you the following items: Copies 3 4/4/2007 NO. DESCRIPTION Second Post-Construction Water Quality Toxicity Testing Laboratory Results for the Carlsbad Golf Course, February 26, 2007 sampling THESE ARE SENT as checked below: For approval X For your use Other As requested For review and comment Remarks: Enclosed are the second "post-construction" toxicological laboratory results for the water quality testing program for the golf course. This testing was done following a rainstorm. The next sampling wi|l be around the end of May or early June, depending on weather conditions. Copy to: John CahNI^City of Carlsbad Signed: