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Home My WebLink About; Carlsbad Municiapl Golf Course Toxicity Testing; Carlsbad Municiapl Golf Course Toxicity Testing; 2009-05-01EnviroMatrix 1 J Analytical, Inc. 01 May 2009 Dudek-& Associates, Inc. "EMA Log #: 0904599 Attn: Chris Oesch 605 Third Street Encinitas, CA 92024 ProjectName: CARLSBAD GOLF COURSE Enclosed are the results of analyses for samples received by the laboratory on 04/22/09 15:39. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. 1 certify that this data is in compliance both technically and for completeness. Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewridge Avenue, Suite A - San Diego, California 92123 - (858) 560-7717 - Fax (858) 560-7763 Analytical Chemistry Laboratory Client Name: Dudek & Associates, Inc. EMA Log #: 0904599 ProjectName: CARLSBAD GOLF COURSE ANALYTICAL REPORT FOR SAMPLES Sample ID Laboratory ID Matrix Date Sampled Date Received DOWNSTREAM 0904599-01 Water 04/22/09 08:30 04/22/09 15:39 UPSTREAM, . . 0904599-02 . .Water;. .04/22/09 09:45 04/22/09 15:39 WELLS 0904599-03 Water 04/22/09 09:00 04/22/09 15:39 WELL 1 0904599-04 Water 04/22/09 11:00 04/22/09 15:39 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix [ jAnalytical, Inc. '. Page 2 of 8 Client Name: Dudek & Associates, Inc. Project Name: CARLSBAD GOLF COURSE EMA Log #: 0904599 Conventional Chemistry Parameters by Standard/EPA Methods Analyte Result Reporting Limit Units Dilution Batch Prepared Analyzed Method Notes DOWNSTREAM (0904599-01) Water Sampled: 04/22/09 08:30 Received: 04/22/09 15:39 - •• - - -— Nitrate as N 0.18 0.05 mg/l 1 9042304 04/23/09 04/23/09 SM4500NO3E Nitrite as N ND 0.05 " 9042303 04/23/09 04/23/09 SM4500 N02 B Dissolved Oxygen 6.32 0.10 " 9042919 04/22/09 04/22/09 S.M4500-O G HT-15 pH 7.54 0.10 pH Units 9042301 04/22/09 04/22/09 SM4500-H+ B HT-15 Phosphorus, Total 0.97 0.05 mg/l 9042826 04/27/09 04/27/09 SM4500 P B, E Total Suspended Solids ND 20.0 9042836 04/28/09 04/29/09 SM2540 D UPSTREAM (0904599-02) Water Sampled: 04/22/09 09:45 Received: 04/22/09 15:39 Nitrate as N 0.13 0.05 mg/l 1 9042304 04/23/09 04/23/09 SM4500 N03 E Nitrite as N ND 0.05 9042303 04/23/09 04/23/09 SM4500NO2B Dissolved Oxygen 6.67 0.10 9042919 04/22/09 04/22/09 SM4500-O G HT-15 pH 7.62 0.10 pH Units 9042301 04/22/09 04/22/09 SM4500-H+ B HT-15 Phosphorus, Total 0.28 0.05 mg/l " 9042826 04/27/09 04/27/09 SM4500 P B, E Total Suspended Solids ND 20.0 " 9042836 04/28/09 04/29/09 SM2540 D WELL 3 (0904599-03) Water Sampled: 04/22/09 09:00 Received: 04/22/09 15:39 Nitrate as N 18.7 1.25 mg/l 25 9042304 04/23/09 04/23/09 SM4500 N03 E Nitrite as N ND 0.05 1 9042303 04/23/09 04/23/09 SM4500 N02 B Dissolved Oxygen 3.79 0.10 " " 9042919 04/22/09 04/22/09 SM4500-O G HT-15 pH 6.64 0.10 pH Units 9042301 04/22/09 04/22/09 SM4500-H+ B HT-15 Phosphorus, Total 0.60 0.05 mg/l 9042826 04/27/09 04/27/09 SM4500 P B, E Total Suspended Solids 346 20.0 " " 9042836 04/28/09 04/29/09 SM2540 D WELL 1 (0904599-04) Water Sampled: 04/22/09 11:00 Received: 04/22/09 15:39 Nitrate as N 0.09 0.05 mg/l 1 9042304 ,04/23/09 04/23/09 SM4500 N03 E Nitrite as N ND 0.05 " 9042303 04/23/09 04/23/09 SM4500 Nd2 B Dissolved Oxygen 5.47 0.10 9042919 04/22/09 04/22/09 SM4500-O G HT-15 pH 6.10 0.10 pH Units " 9042301 . 04/22/09 04/22/09 SM4500-H+ B HT-15 Phosphorus, Total 0.75 0.05 mg/l " 9042826 04/27/09 04/27/09 SM4500 P B, E Total Suspended Solids 186 20.0 " 9042836 04/28/09 04/29/09 SM2540 D 77ie results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix [ /ia^ JAnalytical, Inc. Page 3 of8 Client Name: Dudek & Associates, Inc. Project Name: CARLSBAD GOLF COURSE EMA Log #: 0904599 Conventional Chemistry Parameters by Standard/EPA Methods - QuaUty Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 9042301 Duplicate (9042301-DUPl) Source: 0904563-02 Prepared & Analyzed: 04/22/09 pH 7.94 0.10 pH Units 7,96 0,3 20 Reference (9042301-SRMl) Prepared & Analyzed: 04/22/09 pH 9.02 0.10 pH Units 9,10 99 97-102,96 Batch 9042303 Blank (9042303-BLKl) Prepared & Analyzed: 04/23/09 Nitrite as N ND 0.05 mg/l , LCS (9042303-BSl) Prepared & Analyzed: 04/23/09 Nitrite as N 0.10 0.05 mg/l 0.100 105 80-120 LCS Dup (9042303-BSDl) Prepared & Analyzed: 04/23/09 Nitrite as N 0.10 0.05 mg/l 0,100 102 80-120 3 20 Duplicate (9042303-DUPl) Source: 0904599-01 Prepared & Analyzed: 04/23/09 Nitrite as N ND 0,05 mg/l ND 20 Matrix Spike (9042303-MSl) Source: 0904599-01 Prepared & Analyzed: 04/23/09 Nitrite as N 0.12 0.05 mg/l 0.100 ND 117 80-120 Matrix Spike Dup (9042303-MSDl) Source: 0904599-01 ; Prepared & Analyzed: 04/23/09 Nitrite as N ' 0.12 0.05 mg/l 0,100 ND 120 80-120 3 20 Batch 9042304 Blank (9042304-BLKl) Prepared & Analyzed: 04/23/09 Nitrate as N ND 0.05 mg/l The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix f M JAnalytical, Inc. Page 4 of 8 Client Name: Dudek & Associates, Inc. Project Name: CARLSBAD GOLF COURSE EMA Log #: 0904599 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %RBC Level Result %REC Limits RPD RPD Limit Notes Batch 9042304 LCS (9042304-BSl) Prepared & Analyzed: 04/23/09 Nitrate as N 0.46 0.05 mg/l 0.500 93 80-120 LCS Dup (9042304-BSDl) Prepared & Analyzed: 04/23/09 Nitrate as N 0.47 0.05 mg/l 0.500 94 80-120 1 20 Duplicate (9042304-DUPl) Source: 0904551-01 Prepared & Analyzed: 04/23/09 Nitrate as N 3.24 0.25 mg/l 3.12 4 20 Matrix Spike (9042304-MSl) Source: 0904597-01 Prepared & Analyzed: 04/23/09 Nitrate as N 0.44 0.05 mg/l 0,500 ND 89 80-120 Matrix Spike Dup (9042304-MSDl) Source: 0904597-01 Prepared & Analyzed: 04/23/09 Nitrate as N 0.46 0.05 mg/l 0,500 ND 93 80-120 4 20 Reference (9042304-SRMl) Prepared & Analyzed: 04/23/09 Nitrate as N o:7i 0.05 mg/l 0.688 1 04 30.12-107. 7 Batch 9042826 Blank (9042826-BLKl) Prepared & Analyzed: 04/27/09 Phosphoms, Total ND 0.05 mg/l LCS (9042826-BSl) Prepared & Analyzed: 04/27/09 Phosphorus, Total 0.56 0.05 mg/l 0.500 111 80-120 LCS Dup (9042826-BSDl) Prepared & Analyzed: 04/27/09 Phosphorus, Total 0.45 0.05 mg/l 0.500 90 80-120 20 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. ' EnviroMatrix! /iMt JAnalytical, Inc Page 5 of 8 Client Name: Dudek & Associates, Inc. Project Name: CARLSBAD GOLF COURSE EMA Log #: 0904599 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 9042826 Duplicate (9042826-DUPl) Source: 0904429-03 Prepared & Analyzed: 04/27/09 Phosphorus, Total 0.13 0.05 mg/l 0.12 11 20 Matrix Spike (9042826-MSl) Source: 0904429-03 Prepared & Analyzed: 04/27/09 Phosphorus, Total 0.56 0.05 mg/l 0.500 0,12 88 80-120 Matrix Spike Dup (9042826-MSDl) Source: 0904429-03 Prepared & Analyzed: 04/27/09 Phosphoriis, Total 0.53 0.05 mg/l 0,500 0,12 82 80-120 5 20 Batch 9042836 Blank (9042836-BLKl) Prepared: 04/28/09 Analyzed: 04/29/09 Total Suspended Solids ND 20.0 mg/l Duplicate (9042836-DUPl) Source: 0904586-03 Prepared: 04/28/09 Analyzed: 04/29/09 Total Suspended Solids 537 20.0 mgfl 547 2 20 Duplicate (9042836-DUP2) Source: 0904618-01 Prepared: 04/28/09 Analyzed: 04/29/09 Total Suspended Solids 10.0 20.0 mg/l no 10 20 Reference (9042836-SRMl) Prepared: 04/28/09 Analyzed: 04/29/09 Total Suspended Solids 35.5 20.0 mg/l . 34,1 104 9,76-107,3: Batch 9042919 Duplicate (9042919-DUPl) Source: 0904599-01 Prepared & Analyzed: 04/22/09 Dissolved Oxygen 6.29 0.10 mg/l 6,32 . 0,5 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix f M JAnalytical, Inc. Page 6 of 8 Client Name: Dudek & Associates, Inc. Project Name: CARLSBAD GOLF COURSE EMA Log #: 0904599 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 9042919 Duplicate (9042919-DUP2) Source: 0904599-02 Prepared & Analyzed: 04/22/09 Dissolved Oxygen 6.84 0.10 mg/l 6.67 3 20 Duplicate (9042919-DUP3) Source: 0904599-03 Prepared & Analyzed: 04/22/09 Dissolved Oxygen 4.01 0.10 mg/l 3,79 6 20 Duplicate (9042919-DUP4) Source: 0904599-04 Prepared & Analyzed: 04/22/09 Dissolved Oxygen 5.53 0.10 mg/l 5,47 1 20 The resuUs in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix ^^M^ Analytical, Inc. Page 7 of 8 Client Name: Dudek & Associates, Inc. EMA Log #: 0904599 ProjectName: CARLSBAD GOLF COURSE Notes and Definitions HT-15 This sample was received outside of the EPA's recommended 15 minute holding time for this analysis. However, the sample was analyzed immediately upon receipt. .ND ..AnalyteNOTDETECTED.atorabowthereportinglimit NR Not Reported dry Sample results reported on adry weight basis RPD Relative Percent Difference The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix [ /M JAnalytical, Inc. : Page 8 of 8 CHAIN-OF-CUSTODY RECORD Page EMALOGJj Client: - EnviroMatrix V^^^ ^ nalytical. Inc. 4340 Viewridge Ave,, Ste, A - San Diego, CA 92123 - Phone (858) 560-7717 - Ka\ (858) 560-7763 Requested Analysis of Attn: Cufij STO (^^0^ Oe<>CM Samplers(s): QfeSC-H Tl f^ga^LA K^jT Address: ^pS' -"CUil^ Phone: Fax: Email: Billing Address: ProjectlD: CAtUSg^P f-jC^L^ Oc^U'^g Projecl #: P0#: Client Sample ID Sample Date Sample* Time Sample Matrix Container # / Type CJ •s XI (J /3 4/-^7 10 Matrii Codes: A =• Air, DW = Drinking Water, GW = Groundwater. SW = Storm Water WW •= Wastewater, S ° Soil. SED » Sediment, SD - Solid, T » Tissue, 0 •• Oil, L - Liquid RELINQUISI-IED BY Shipped By; ]^ourier oUPS • FedEx • USPS n Clienl Drop Off o Other Signature j::^(ZfJ-pSfi/^ CT^'^ DATE/TIME 'l\im-Around-Tlme: g Same Day • 24 hr Q 48 hr D 3 day o 4 day • 5 day ^STD (7 day) Print c-K^/brtP^^feu C)&cy Company Company 'Repotting Rcqidreinents; • Fax oPDF • Excel • Geolracker/EDF g Hard Copy oEDT 'Saniple MspoMl: jgBy Laboralory •'Return lo Clienl: Pfl) of Delivery- • Archive Signal Sample Integrit)-Company: Correct Containers: Yes No N/A Custody Seals Intact Yes No N/A COCa.abels Agree: Yes No N/A Signature Print Company: Containers Properly Preseved: Yes No N/A Signature Signature Temp @ Receipl:_ Print Print Sampled By: Client EMA Autosampler Company: Company: Project/Sample Comments: Uliite- EMA Canary - Accounting Pink-Client(w/report) Gold - Client (Relinquish) 'Additional costs may apply, consult a project manager for details. ^EMA reserves the ri^t to return any samples lhal do nol match our waste profile. NOTE: By relinquishing samples to EMA, Inc., client agrees to pay for the services requested on this CCX: fomi and any additional analyses performed rai this project. Paymeni for services is due within 7>^ days from date of invoice. Samples will be disposed of after 30 days unless otherwise noted. All work is subject to EMA's terms and conditions. I Results of Toxicity Testing for The City of Carisbad - Municipal Golf Course Water Quality Study Testinq Period: April 2009 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 June 3, 2009 Data Qualitv Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmentai Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as ail minimum acceptability criteria for test controls under the EPA protocol requirements. • Ali data have been reviewed and verified. Results Verified By Date: ^-5-^7 TOXICITY SUMMARY REPORT Test ID Number: 0904-8090 to -S093 Client: Dudek & Associates Monitoring Period: April 2009 Sample Information Client: Project Study: .Sample Site ID's: Sample Collection Date, Time: Sample Receipt Date, Time: Sample Material: Dudek & Associates, Inc. Carlsbad Golf Course Toxicity Evaluation - January 2009 a) Downstream b) Upstream c) Well #3 d) Well #1 4/22/09, 08:30 to 11:00 4/23/09, 10:30 Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Control: Protocol Used: Test Acceptability Criteria: 4/23/09 to 6/1/09 Ceriodaphnia dubia (water flea) 7 or 8-day chronic survival and reproduction In-house culture, < 8 hours at initiation 100, 50, 25,12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity = 200 |jS/cm Lab freshwater spiked with seawater to raise the conductivity to match the sample's conductivity levels, which roughly ranged from 3,000 to 5,000 |jS/cm. This control was conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Table 1. Water Quality Information: Samples Upon Receipt Sample ID pH DO (mg/L) Temp (°C) Conductivity ()iS/cm) Hardness (mg/L as CaCOs) Alkalinity (mg/L as CaCOa) Downstream 7.64 8.0 4.8 3786 >1000 245 Upstream 7.57 8.1 5.8 4170 >1000 189 Well #3 6.59 5.1 5.8 5020 >1000 241 Weil #1 6.35 5.9 4.9 3087 757 45 Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Test ID Number: 0904-S090 to -3093 Ciient: Dudek & Associates Monitoring Period: April 2009 Table 2. Summary of Test Results Concentration (%) Mean Percent Mean Number of Sample ID Concentration (%) Survival Offspring Produced Lab Control 100 15.8 Downstream 6.25 100 21.5 12.5 90 21.4 25 100 • 23.1 50 100 20.5 100 90 17.1 Cond. Control 60 * 6.8* Lab Control \ 100 15.8 Upstream 6.25 80 15.8 12.5 100 21.9 25 90 17.6 50 100 20.1 100 100 15.8 Cond. Control 70 1.2* Lab Control ^ 100 15.8 Well #3 6.25 100 21.5 12.5 80 20.7 25 .90 19.7 50 70 16.4 100 40* 1.5* Cond. Control 60* 3.4 * Lab Control ^ 100 15.8 Well#1 6.25 100 18.6 12.5 100 17.0 25 100 19.7 50 100 21.2 100 100 10.7 Cond. Control 100 16.6 1 The Lab Control conducted with the Downstream sample was used in data analysis for all sannples testeiJ. * An asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control. Note: The Conductivity Control was lab water spiked to match the conductivity of the 100% sample, and was used as a comparison to determine whether conductivity levels had an effect on the final results. The approximate conductivity levels of each sample were as follows: 3800 \iSlcm (Downstream); 4200 pS/cm (Upstream); 5000 pS/cm (Well #3); and 3100 pS/cm (Well #1). Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates TaWe 3. Summary of Statistical Results and Reportable Values Sample ID NOEC ECso TUc Test & Endpoint (% effluent) , (% effluent) (toxicity units) Downstream: Survival 100 >100 1.0 Reproduction 100 >100 1.0 ypstream: Survival 100 >ioo 1.0 Reproduction 100 > 100 1.0 Well #3: Survival 50 79:4 2.0 Reproduction . 50 72.4 2.0 Well#1: Survival 100 > 100 1.0 Reproduction 100 >100 1.0 NOEC = the highest Concentration that produces No Observed Effect ECso = concentration that causes a 50% adverse effect to the test organisms Chronic Toxicity Units (TUc) = 100. NOEC Discussion of Results One lab control, tested concurrently with the Downstream sample, was used for all data analyses and comparisons. This control met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. There was no toxicity observed with the Upstream, Downstream, and Well #1 samples. However, the Well #3 sample did show toxicity to both survival and reproduction at the 100% concentration. The adverse effects seen in the Well #3 sample was somewhat expected, due to the elevated levels of conductivity found in the samples. The Weil #3 sample had the highest conductivity level (about 5,000 ^lS/cm) of the four samples being tested. From internal studies and other references, we normally see adverse effects to the reproduction endpoint when conductivity levels exceed approximately 2,500 (jS/cm. Conductivity is related to fhe concentration of different salts and ions in a sample. The optimum range for Ceriodaphnia is between approximately 100 and 1,500 jjS/cm. Therefore, the high conductivity levels found in the Well #3 sample may have contributed to the toxicity observed in the test However, it was interesting that the other three samples all had conductivity levels exceeding 2,500 pS/cm (ranging from 3,100 to 4,200 pS/cm), but none of the three resulted in toxic effects. Each of the conductivity controls, which matched the conductivity level of the sample, produced the toxic effects that were expected. The fact that, the other three samples produced no toxic effects, meant that there was most likely something in the sample makeup that was inhibiting the toxic effects and allowing the organisms to survive and reproduce normally atthe higher conductivity levels. Nautilus Environmental - San Diego Bioassay Laboratory Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis Site: Downstream CETIS Summary Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Link/Link Code: ,29 May-09 15:28 (p 1 of 2) 03-2684-1851/0904-S093 Nautilus Environmental (CA) Test Run No: 07-9925-0454 Test Type: Reproduction-Survival (7d) StartDate: 23 Apr-09 14:30 Protocol: EPA/821/R-02-013 (2002) Ending Date: 01 May-09 13:20 Species: Ceriodaphnia dubia Duration: 7d 23h Source: In-House Culture Analyst: Diluent: Brine: Age: Diluted Mineral Water (8:2) Not Applicable <8hr Sample No: 17-9916-2486 Sample Date: 22 Apr-09 08:30 Receive Date: 23 Apr-09 10:30 Sample Age: 30h (4.8 "C) Code: 09-0327 Material: Downstream Ambient Sample Source: Dudek & Associates station: Downstream Client: Project: Dudek & Associates Comparison Summary Analysis No Endpoint NOEL LOEL TOEL 00-8676-7941 7d Survival Rate 12-4343-2810 Reproduction PIHSD Method 100 100 > 100 > 100 N/A N/A N/A Fisher Exact/Bonferroni-Holm Test 35.5% Steel Many-One Rank Test Test Acceptability Analysis No Endpoint Attribute Test Stat Acceptabilty Limits Overlap Yes Yes Yes Decision 00-8676-7941 7d Survival Rate 12-4343-2810 Reproduction 12-4343-2810 Reproduction Control Resp Control Resp PMSD 1 15.8 0.355 0,8-NL 15-NL 0.13-0.47 7d Survival Rate Summary Conc-% Control Type Count Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Mean 95% LCL 95% UCL Min 0 0 6.25 12.5 25 50 100 Conductivity C Lab Confrol Max 10 10 10 10 10 10 10 0.6 1 1 • 0.9 1 1 0.9 Std Err Std Dev CV% 0,407 1 1 0.782 1 1 0.782 0.793 0 1 1 0 1 1 0 0,0943 0 0 0.0577 0 0 0,0577 0.516 0 0 0.316 0 0 0.316 86.1% 0.0% 0.0% 35.1% 0,0% 0.0% 35.1% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Diff% 0.0% -66,7% -66.7% . -50.0% -66.7% -66.7% -50,0% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Dlff% Conductivity C 10 6,8 4.13 9.47 0 19 1.31 7.16 105,0% 0,0% Lab Control , 10 15,8 13.8 17.8 5 25 0.992 5,43 34.4% -132,0% 10 21,5 20,1 22.9 18 30 0.662 3.63 16.9% -216.0% 10 21.4 19 23.8 6 27 1.16 , 6.38 29.8% -215.0% 10 23.1 22 24.2 18 27 0.561 3.07 13.3% -240.0% 10 20.5 18.1 22.9 6 28 1.17 , 6,42 31.3% -201.0% 10 17.1 14.6 19.6 0 24 1.24 6.81 39.8% -151,0% 000-089-163-3 CETIS™ v1,6.3revE Analyst I CETIS Summary Report Report Date: Link/Link Code: 29 May-09 15:28 (p 2 of 2) 03-2684-1851/0904-S093 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Reps Rep 9 Rep 10 0 Conductivity Co 0 0 1 1 1 1 0 1 1 0 0 Lab Control 1 • 1 1 1 1 1 1 1 1 1 6.25 1 1 1 1 1 1 1 1 1 1 12.5 1 1 1 1 1 0 1 1 1 1 25 1 1 1 1 1 1 1 1 1 1 50 1 1 1 1 1 1 1 1 1 1 100 0 1 1 • .1 1 1 1 1 1 Reproduction Detail Conc-% Contro) Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Coiiductivlty Co 0 0 17 11 10 4 0 6 19 1 0 Lab Confrol 19 13 16 25 12 17 5 18 20 13 6.25 21 25 19 21 18 22 21 20 18 30 12.5 22 17 19 26 21 6 24 25 27 27 25 23 18 20 24 21 24 26 21 27 27 50 23 22 24 13 6 19 24 28 23 23 100 0 17 15 14 21 19 18 24 20 23 000-089-163-3 CETIS™ v1.6,3revE Analyst: 1)^ QA: CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 15:28 (pi of 1) 03-2684-1851/0904-S093 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 00-8676-7941 Endpoint: 7d Survival Rate CETIS Version: CETlSv1.6.3 Analyzed: 29 May-09 15:27 Analysis: STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed • C>T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% Test Stat P-Value Decision(0.05) Lab Control 6.25 1 1 Non-Significant Effect 12.5 0.5 1 Non-Significant Effect 25 1 1 Non-Significant Effect 50 1 1 Non-Significant Effect 100 0.5 1 Non-Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 6.25 12.5 25. 50 100 Lab Control 10 10 9 10 10 9 10 10 10 10 10 10 7d Survival Rata Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control Graphics , 1,0- 0,9- u 0:8- o: 0.7- •|, 0.6- w A.S-'O 0.4- 0.3- 0.2- 0.1- 0.0 12.5 25 Conc-% 000-089-163-4 CETIS™ v1.6.3revE Analyst:_ CETIS Analytical Report ReportDate: Link/Link Code: 29 May-0915.28 (p 1 of 2) 03-2684-1851/0904-S093 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 12-4343-2810 Endpoint: Reproduction CETIS Version: CETISvl.6.3 Analyzed: 29 May-09 15:27 Analysis: Nonparametric-Contro! vs Treatments Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Rank C>T Not Run 100 >100 N/A 1 35.5% steel Many-One Rank Test Control vs Conc-% Test Stat Critical Ties P-Value Decision(5%) Lab Control 6.25 140 75 4 1.0000 Non-Significant Effect 12.5 136 75 3 1.0000 Non-Significant Effect 25 145 75 2 10000 Non-Significant Effect 50 131 75 2 1.0000 Non-Significant Effect 100 118 75 4 0.9840 Non-Significant Effect ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Decision(5%) Between 400.6 80.12 5 2.67 0.0317 Significant Effect Error 1622.8 30.05185 54 Total 2023.4 110.1719 59 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%) Variances Bartlett Equality of Variance 8.21 15.1 . 0.1450 Equal Variances Distribution Shapiro-Wilk Normality 0.904 0.0002 Non-normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% DifP/o 0 Lab Control 10 15.8 13.7 17.9 5 25 1.01 5.43 34.4% 0.0% 6.25 10 21.5 20.1 22.9 18 30 0.674 3.63 16.9% -36.1% 12,5 10 21.4 19 23.8 6 27 1.18 6.38 29.8% -35.4% 25 10 23.1 21.9 24.3 18 27 0.57 3.07 13.3% -46.2% 50 10 20.5 18.1 22.9 6 28 1.19 6.42 31,3% -29.7% 100 10 17.1 14.5 19.7 0 24 1.26 6.81 39,8% -8,23% Rank Transformed Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% DifP/o 0 Lab Control 10 16.2 10.6 21.7 2 51 , 2.69 14.5 89,6% 0.0% 6.25 10 32.7 27.4 37.9 17 60 2.59 13.9 42.7% -102.0% 12:5 10 37.2 29.9 44.4 • 3.5 56.5 3.54 19.1 51.3% -130.0% 25 10 40.8 35.7 45.9 17 56.5 2.51 13.5 33.1% -153.0% 50 10 34.5 27.6 41.3 3.5 59 3.33 17.9 52.1% -113.0% 100 10 21.8 16.2 27.4 1 46.5 2.73 14,7 67.4% -35.0% 000-089-163-4 CETIS™ v1.6.3revE Analyst:. 1£_ QA^ CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 15:28 (p2of 2) 03-2684-1851/0904-8093 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 12-4343-2810 Analyzed: 29 May-09 15:27 Endpoint: Reproduction Analysis: Nonparametric-Control vs Treatments CETIS Version: CETISv1.6.3 Official Results: Yes Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 25 20 19 18 17 16 13 13 12 5 6.25 30 25 22 21 21 21 20 19 18 18 12.5 27 27 26 25 24 22 21 19 17 6 25 27 27 26 24 24 23 21 21 20 18 50 28 24 24 23 23 23 22 •19 13 6 100 24 23 21 20 19 18 17 15 14 0 Graphics 5 30- 1 12.S 25 Conc-% 100 10 30- 20 10- 0 -JO -20- -30- -40- -2.5 -2.0 -l.s -1.0 -O.S 0.0 0.5 1.0 1.5 2.0 2.5 Rankits 000-089-163-4 CETIS™ v1,6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 15:32 (p1 of 1) 03-2684-1851 /0904-S093 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 08-6396-4057 Endpoint: 7d Survival Rate Analyzed: 29 May-09 15:31 Analysis: Single 2x2 Contingency Table CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run <0 0 N/A N/A Fisher Exact Test Control vs Control Test Stat P-Value Decision(0.05) Lab Control Conductivity Control 0,0433 0.0433 Significant Effect Oata Summary Conc-% Control Type No-Resp Resp Total 0 Conductivity Co 6 0 Lab Control 10 4 0 10 10 7d Survival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 6 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 1 0 Lab Control 1 1 1 1 1 1 1 1 • 1 1 1 0 1 0 1 0 1 0 1 Graphics 1.0- 0.9^ il 0.8- & 0.7- > e 0.6- a 0.5- 0.4- 0.3- 0.2- 0,1- 0.0 0 0 000-089-163-4 CETIS™ v1.6.3revE Analyst: QA: £0 CETIS Analytical Report 1 A 1 ^ ReportDate; .i^iviuy-uc? nj.oi i i^i •/ t-oL ColCT«>' LQAHt)t ) Link/Link Code: 03-2684-1851/0904-S093 29 May-09 15:31 (p 1 of 1) Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysiis No: 12-2947-8979 Analyzed: 29 May-09 15:31 Endpoint: Reproduction Analysis: Parametric-Two Sample CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransfonned C>T Not Run <0 0 N/A 31.2% Equal Variance t Two-Sample Test Control vs Control Test Stat Critical MSD P-Value Decision(5%) Lab Control Conductivity Control 3.17 1.73 4.93 0.0027 Significant Effect ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Decision(S%) Between 405 Error 727.2 405 40.4 1 18 10 0,0054 Significant Effect Total 1132.2 445.4 19 ANOVA Assumptions Attribute Test Test Stat ' Critical P-Value Decision(1%) Variances Variance Ratio F Distribution Shapiro-Wilk Normality 1.74 0.966 6.54 0.4230 0.6770 Equal Variances Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff% 0 Lab Control 10 0 Conductivity Co 10 15.8 6.8 13.7 4.08 17,9 9,52 5 0 25 1.01 19 1,33 5.43 34.4% 7.16 105.0% 0.0% 57.0% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 19 0 Lab Contfol 25 17 20 11 19 10 18 6 17 4 1 16 13 0 0 13 12 0 5 Graphics ••8 iSti c c Reject Null 0 CC Conc-% 0 LC- -2.0 -1.5 -1.0 -0.5 0.0 0.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst; ^l^f QA^ Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates/Downstream Test Number: Q^OM- tjOH '^ Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 / ^.' 3o End Date/Timeu4jp0/20e9 i-S^ I I I I I I Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # mmm 3 •m&m 7 ; 8 Total QC LC 1 17 0 o O o 9 n ' LC 2 6 o o rO ^\ o o •/ • L^ 3 44 o o 0 'i 0 H u 4 39 r. o ij u 2-0 T 5 54 ft o 0 V o \ la 6 56 6 o €> 0 0 -7 17 7 12 , 8 G O 0 o o 8 23 c o 0 0 0 iO /O 9 19 0 G o & b o iO 10 64 O o o •h lO Tech: fA SL Si. Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # ;::::-:*;i;p: 6 Total QC CC 1 8 01 oiA/ _ ---,l/r CC 2 18 0 o _ • — 3 68 6 o 1 o 0 '«•^lc^ 17' 4 34 ft <3 • o « o II 5 66 0 r3 fl 0 o »P 6 24 0 o o 4 7 26 0 O -— -8 11 0 o o o 6 o ti>' 9. 53 O o & o T (Cb 10 28 0 0 o V 0 o -iU-' ... Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # Total QC 6.25% 1 48 0 ,.a ,. o (i 7 o 6.25% 2 70 0 o o a I 6 3.5 3 2 0 o t) 0 H \\ 4 16 0 o o 0 0 •vi 5 21 a o a t O 6 62 0 o O () 0 ier ll 7 50 0 <J7 -o Q A 0 8 38. . <>. O (O (J 1 o to an 9 33 o o 0 u ^ i(^) 10 3 0 o V 0 0 13 Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # :;;;:;3*;; mmm vx;5;:^;:;; ;i;;;r6::;;;:; :::;;:;7:;v-:; Total QC 12.50% 1 58 0 a O 6 a 0 .^A : l\c7. 12.50% 2 69 0 £>. o r) r ^ o 17 3 52 0 O G n VI \\ 4 49 (3 O O (J^ <5 13, 5 7 (5 C O 0 6 6 32 0 O o 0 01 (A.-— — — lo / 7 51 G o r! «+ n I?, 8 40 6 C o 0 H> iO o ii a5 9 1 0 o V 0 0 u lO &7 io 10 6 d o 0 . i-0 q iH Neonates for each replicate were blocked across concentrations at test initiation Rep; Board: Cup; Verified By: 1 2 3 4 5 6 7 8 9 10 Rep; Board: Cup; Verified By: as a% 3a Rep; Board: Cup; Verified By: 5 Rep; Board: Cup; Verified By: Time- Fed/Test Solution Renewed (day): ^ (0)(M^& <i)Mno (2)_i%£_(3)_\^:^_(4)J:£iV_tsi l^HO (6) \Q5" {^j^^ Notes: d = dead; IVl = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) QC Check; .Df ^ |u|0'^ Final Review: 5'\T^\O^ Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates/Downstream Test Number: Q^DM- .^"^ ?) Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 3 C> End Date/Time: 4/30/2609 -rffSO /3>0 Conc. Rep Rand # , Daily Reproduction/ Survival Total i QC Conc. Rep Rand # 4 •7 8 Total i QC 25% 1 31 O o O o 25% 2 57 • ® o* o O 3 o u 1^ 3 20 0 o 0 u o 4 13 0 c o n fc • o 5 41 6 o o 0 5 ^1 6 45 D o Q H q o H . 7 9 0 o o 0 0 \'i 8 35 0 o o 0 \ 9 30 0 o 0. y VO o 10 55 0 O 0 0 0 Tech: Pi!. St SL Conc. Rep Rand # Dally Reproduction/ Survival Total QC Conc. Rep Rand # 'm^-$m :^:,"3"^-,:: 4 mmtA •i; : :8' •• Total QC 50% 1 4 0 6 O o % 50% 2 .37 0 0 <o 3 6 5 I 3 25 0 O O 0 0?. -7 0 W 1^4 4 46 0 o o & o 0 5 42 e O 0 0 0 d o 6 65 6 o p o 1 7 61 n o o n 0 •7 8 43 b O-0 '^ I 10 o 9 5 0 o 0 0 . -1 0 10 14 0 <5 0 0 -o: Conc. Rep Rand # Daily Reproduction/Survival Total QC ) Conc. Rep Rand # Mmi0\ ;;:;i;2i:;^;; m.0B 4: Total QC ) 100% 1 47 0 — — — — • —• 100% 2 67 0 O o 5 o 17' 3 27 h O 0 1) o lb 4 -22 o 6 0 5 6 5 59 0 Q o c; 1 o 5 60 0 o o (? H o w 7 36 d o 0 0 3> 9; 1? 8 63 0 O 0 0 a 9 29 • i") <3 t:^ • <d -7 0 10 15 <? c. V 0 2— 0 1 vH Neonates for each replicate were blocked across concentrations at test initiation" Rep:[ Board; Cup: Verified By: 1 2 3 4 5 7 8-9 10 j 3^ 1 ^ 1 Tiine Fed/Test Solution Renewed (day (01 (Unno (2)_Jl^^ (3). (4) SO (5)\2^ (6) JOS^ Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included In total) .(7). QC Checl<:Df 5 Nautilus Environmen Final Review: W^1/<^ at 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client: Dudek Sample ID: Downstream Water Quality Measurements Test Species: C. dubia Start Date/Time: 4/23/2009 1?0 Test No: Ci=\C>^ - SQ^JS End Date/Time: 4/^pi^09 \'53 0 Concentration Lab Control Day 0|l|z|3|4l5|6|7 fP initial PH ) 7-SO JM. :> DO (mq/L) -7.1 1 u 7. ^ 7.-5 7- b JM. :> Cond. (pmhos/cm) ^q<t \h\ ^0 . JM. :> Temp (°C) 2-M.2- J JM. :> •Final pH B..2vf DO (mq/L) ^ .a 7 <i S.l Temp |°C) 24 3 3^.5 Concentration Conductivity Control Day 0il|2|3|4|5l6i7 ' ' ' iilitial pH ^-12--7 .'8,'=, 7-7& «.io ^. U DO (mq/L) •7.-7 7-f S'.LP 7.1 7. b ^. U Cond. (pmhos/cm) 3_-7itl_ 3700 P>7^^ ^. U Temp (°C) •2.H.Z ^. U " „. ^ • < ..J'inal PH «'c.|5 ir 67 TT DO (mg/L) ^'^ ^4 %.\ -TT Temp CO -54-1 ^4-3 Concentration 6.25% Oay 0 1 1 1 2 | 3 1 4 ! 5 I s l 7 initial pH 7. ?2 D0(mq/L) 7-a n.y 7-<b Cond. (pmhos/cm) t<il5 MM? Temp CO 25.& 25.7 ^,H^1 >< pH /'"^"^ 8,13. DO (mq/L) Teitip (°C) 2H,T Concentration 12.6% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 Initial pH ~}^^ 7Tf7 %-S>b •'tis DO (mg/L) 7-7 -7.7 11 •'tis Cond. (Mmhos/cm) 7(2^ 74-Z 7ZO li\J •'tis Temp ro ^ ^> •26-'2 •'tis Final pH Ml DO (mq/L) S", a.. Temp ("O 01 2 3 4 5 6 7 Analysts: Initial: Final; Sample Used (A, B. C): HO es Mi) ~ 1 Analysts: Initial: Final; Sample Used (A, B. C): ma SL Analysts: Initial: Final; Sample Used (A, B. C): A-4 IV illii Comments: Animal Source/Date Received: Vv^ACTVyM I KIA Sample Log-in Numbers: A: Animal Age at lnifiation:^jQJ^3_1^2- C: — QC Chock; T)F 5|\ol(j)ci Nautilus Einironrnemal 5550 Morelwusa Drive, Suite 150. Sai\ Diago, CA 92121. Final Review: I Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek Test Species: C. dubia Sample ID: Downstream Start Date/Time: 4/23/2009 Test No: End Date/Time: ApeitSoa 13*3^ 6'h \tA pp- Concentration 25% Day 0I1I2I3I4ISI 6I7 pH "Ifll 77^ S.oj AH,7 00 (mq/L) 7 7 7.1 T<? AH,7 Cond. (fjmhos/cm) 1777 I3.)b 1?)\7 AH,7 Temp(°C) ^ (|5 Z5i AH,7 s» pH DO (mq^L) Temp (°C) —. 1 »—• ^3fi7 xq-3 Concentration 50% Day 0 1 1 1 2 1 3 1 , 4 i 5 1 6 1 7 pH 174 ••i.'6 DO (mq/L) 7?) ••i.'6 Cond. (pmhos/cm) lace XI Q O -HOC) ••i.'6 Temp CC) 35. ••i.'6 pH 00 (mc(/L) ^.^ Temp CC) 81-7 :^M'3 Concentration 100% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 Initial pH -7-7 7C^^7 7X 7 MMi DO (mq/L) 7 MMi COIKI. (pmhos/crn) 37H7 3\?\733 3S7D ^7ion rqooo 7 MMi Temp (°C) 0.5-3 7 MMi pH '?.'^( 3-^7 ^. so DO (mq/L) ^4 & W Temp (°C) a,4,{) 0 1 2 3 4 5 6 7 Analysts: Initial: Final: Sample Used (A, B, C): NO ilii -— Analysts: Initial: Final: Sample Used (A, B, C): SL '(^ HP SL Analysts: Initial: Final: Sample Used (A, B, C): A-4r A-A liie: ^— \ Comments; Animal Source/Date Received: (m'^yWO-V Sample Log-in Numbers: A: 0*^ " OT^ 2-^ B: 00 Check; Animal Age at Initiation: C; Final Review; Nautilus Environmental 5550 /Morehouse Drive, Suite 150. Sun Dingo. CA 92121. Site: Upstream I I I CETIS Summary Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Link/Link Code: 29 May-09 16:04 (p 1 of 2) 07-2893-1117/0904-S092 Test Run No: Start Date: Ending Date: Duration: 10-4321-9131 23 Apr-09 14:30 01 May-09 09:25 7d 19h Test Type: Reproduction-Survival (7d) Protocol: EPA/821 /R-02-013 (2002) Species: Ceriodaphnia dubia Source: In-House Culture Nautilus Environmental (CA) Analyst: Diluent: Brine: Age: Diluted Mineral Water (8:2) Not Applicable <8hr Sample No: 17-9103-9974 Code: Sample Date: 22 Apr-09 09:30 Material: Receive Date: 23 Apr-09 10:30 Source: Sample Age: 29h (5.8 °C) Station: 09-0326 Dudek & Associates Upstream Client- Project: Dudek & Associates Comments: Comparison Summary Analysis No , Endpoint .. The lab control from the Downstream testdD# 0904-3093) was shared and used fordata analysis and comparisons.' 09-0777-1421 14-7132-3282 7d Survival Rate Reproduction NOEL LOEL TOEL PMSD Method 100 100 > 100 > 100 N/A N/A Test Acceptability Analysis No Endpoint N/A Fisher Exact/Bonferronl-Holm Test 37.4% Steel Many-One Rank Test 09-0777-1421 7d Survival Rate 14-7132-3282 Reproduction 14-7132-3282 Reproduction Attribute Control Resp Control Resp PMSD Test Stat Acceptabilty Limits 1 15.8 0.374 0.8-NL 15-NL 0.13-0.47 7d Survival Rate Summary Conc-% Controt Type Count Overiap Decision Yes Passes acceptability criteria Yes Passes acceptability criteria Yes Passes acceptability criteria 0, 0 6.25 12.5 25 50 100 Conductivity C 10 0.7 0.52 Lab Control 10 i i 10 0.8 0.643 10 1 1 10 0.9 0.782 10 1 1 10 1 1 Mean 95% LCL 95% UCL Min Max Reproduction Summary Conc-% Control Type 0.88 1 0.957 1 1 1 1 0 1 0 1 0 1 1 0 0 6.25 12.5 25 50 100 Conductivity C Lab Control Count 10 10 10 10 10 10 10 Mean 1.2 15.8 15.8 21.9 17.6 20.1 15.8 95% LCL 9S%UCL Min 0,741, 13,8 13 20,8 14.5 18.9 14 1.66 17.8 18.6 23 20.7 21.3 17.6 0 5 0 17 0 14 4 Max 3 25 22 26 26 25 20 0.224 0,992 1.38 0.554 1,52 0.599 0.877 Std Err Std Dev CV% Diff% 0.0882 0.483 69.0% 0.0% 0 0 0,0% -42.9% 0.077 0.422 52.7% -14.3% 0 0 0.0% -42.9% 0.0577 0.316 35,1% -28.6% 0 0 0.0% -42,9% 0 0 0.0% -42.9% Std Err Std Dev CV% 1,23 • 5,43 7.57 3.03 8.34 3.28 4.8 102.0% 34.4% 47.9% 13.9% 47,4% 16.3% 30.4% Diff% 0.0% -1220.0% -1220.0% -1730.0% -1370.0% -1580.0% -1220.0% 000-089-163-3 CET1.S™ vl.6.3revE Analyst: . QA: CETIS Summary Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Link/Link Code: 29 May-09 16:04 (p 2 of 2) 07-2893-1117/0904-S092 Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% Control Type Rep 1 0 0 6.25 T2.5 25 50 100 Conductivity Co 0 Lab Control Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Reproduction Detail Conc-% Control Type Rep 1 Rep 7 1 1 1 1 0 1 1 Rep 8 Rep 9 Rep 10 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 0 Conductivity Co 0 3 . o 1 0 Lab Control 19 13 is 25 6.25 15 16 20 21 ••2.5 23 23 19 26 25 11 21 22 26 50 17 22 21 21 100 18 18 13 20 1 12 5 24 12 24 17 2 17 0 26 25 18 14 0 5 21 17 0 25 20 0 18 22 21 19 19 19 2 20 22 21 14 20 4 Rep 7 Rep 8 Rep 9 Rep 10 3 13 16 19 26 14 15 000-089-163-3 CETIS™ v1,6.3revE Analyst:. QA: /t> CETiS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Link/Link Code: 29 May-09 16:04 (p 1 of 1) 07-2893-1117/0904-5092 Analysis No: 09-0777-1421 Analyzed: 29 May-09 16:01 Endpoint 7d Survival Rate Analysis: STP 2x2 Contingency Tables Nautilus Environmental (CA) CETIS Version: Official Results: CETlSvl.6.3 Yes Data Transform Zeta Untransformed Alt Hyp Monte Carlo NOEL LOEL C>T TOEL Not Run TU 100 >100 PMSD N/A N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100 Test Stat 0.237 1 0:5 1 1 P-Value Decision(0.05) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Oata Summary Conc-% Control Type No-Resp Resp Total 0 6.25 12.5 25 50 100 Lab Control 10 '8 10 9 10 10 0 2 0 1 0 0 10 10 10 10 10 10 7d Survival Rate Detail — Conc.% Control Type Repl Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rap 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control Graphics 1 0 1 1 1 1 1.0-1 0.9- s s 0.8-s s 0.7- 2 g 0.6- 3 Ul 0.5- 0.4- 0.3- 0.2- 0.1- 0.0 - 6.2S 12.5 25 Conc-% 1 0 1 0 1 1 000-089-163-4 CETIS™ v1.6,3revE Analyst:. ^ QA:^ CETIS Analytical Report Report Date: Link/Unk Code: 29 May-09 16:04 (p 1 of 2) 07-2893-1117/0904-S092 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 14-7132-3282 Endpoint: Reproduction CETIS Version: CETISvl.6.3 Analyzed: 29 May-09 16:01 Analysis: Nonparametric-Control vs Treatments Official Results Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Rank OT Not Run 100 >100 N/A 1 37.4% Steel Many-One Rank Test Control vs Conc-% Test Stat Critical Ties P-Value Declslon(5%) Lab Control 6.25 113 75 3 0.9550 Non-Significant Effect 12.5 141 75 2 1.0000 Non-Significant Effect 25 117 75 3 0.9780 Non-Significant Effect 50 133 75 5 1.0000 Non-Significant Effect 100 109 75 5 0.9080 Non-Significant Effect ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Decision(5%) Between 341.3333 68,26667 5 2.05 0.0856 Non-Significant Effect Error 1795 33.24074 54 Total 2136.333 101.5074 59 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%) Variances . Bartlett Equality of Variance 13.9 15.1 0.0162 Equal Variances Distribution Shapiro-Wilk Normality 0.926 0.0014 Non-normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min ; Max Std Err Std Dev CV% Diff% 0 Lab Control 10 15.8 13.7 17.9 5 25 1.01 5.43 34.4% 0.0% 6.25 10 15.8 12.9 18.7 0 22 1.41 7.57 47.9% 0.0% 12.5 10 21.9 20,7 23.1 17 26 0.564 3.03 13.9% -38.6% 25 10 17.6 14.4 20,8 0 26 1.55 8.34' 47.4% -11.4% 50 10 20.1 18.9 21.3 14 25 0.609 3.28 16.3% -27.2% 100 10 15.8 14 17,6 4 20 0.892 4,8 30.4% 0.0% Rank Transformed Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff% 0 Lab Contro! 10 21.9 15,9 27.8 4.5 55 2.9 15,6^ 71.4% 0.0% 6.25 10 27,3 20.5 34 1.5 47.5 3.29 17.7' 65.0% -24.7% 12.5 10 43.7 38.9 48.5 21.5 58.5 2.36 12.7 29.1% -100.0% 25 10 32 23.2 40.8 1.5 58.5 4.31 23.2 72.5% -46.5% 50 10 36.6 31.3 41.8 13 55 2.56 13.8 37.8% -67.3% 100 10 21.7 17.4 25.9 3 36 2.07 11,1 51,4% 0.92"/« 000-089-163-4 CETIS™ v1.6.3revE Analyst: OA: CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 16.04 (p 2 of 2) 07-2893-1117/0904-S092 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 14-7132-3282 Analyzed: 29 May-09 16:01 Endpoint: Reproduction CETIS Version: Analysis: Nonparametric-Control vs Treatments Official Results: CETlSvl.6.3 Yes Reproduction Detail Conc-% Control Type Rep1 > Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 25 20 19 18 17 16 13 13 12 5 6.25 22 22 21 21 20 16 16 15 5 0 12.5 26 26 24 23 23 21 21 19 19 17 25 26 26 25 22 21 19 14 12 11 0 50 25 24 22 21 21 20 19 18 17 14 100 20 20 19 18 18 17 15 14 13 4 Graphics 25- B 20-za 10- 30- 20- £ JC S « 10- = 2 S 0 -10- -20- -30- -40- 12.5 2S Conc-°/o -2.5 -2.0 -l.s -1.0 -O.S 0.0 0.5 1.0 I.S 2.0 2.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst: CETIS Analytical Report Report Oate: Link/Link Code: 29 May-09 16:07 (pi of 1) . 07-2893-1117/0904-S092 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 07-6801-8770 Endpoint: 7d Survival Rate CETIS Version: CETISvl.6.3 Analyzed: 29 May-09 16:02 Analysis: Single 2x2 Contingency Table Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run 0 >0 N/A N/A Fisher Exact Test Control vs Control Test Stat P-Value Declsion(O.OS) Lab Control Conductivity Control 0,105 0.105 Non-Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 , Conductivity Co 7 3 10 0 Lab Control 10 0 10 7d Survival Rate Detail Conc-% Control Type Repl Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C. 1 1 1 1 1 1 1 0 0 0 0 Lab Control 1 1 1 1 1 1 1 1 1 1 Graphics 1.0 0.9- « 0.8- S 0.7 •| 0.6-1 5 0.5-•o 0.4- 0.3 0.2- 0.1- 0.0 0 Conc-% 0 000-089-163-4 CETIS™ v1.6.3revE Analyst: QA: .C CETIS Analytical Report Report Date: 29 May-09 16:07 (p 1 of 1) 07-2893-1117/0904-S092 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 08-2156-3038 Analyzed: 29 May-09 16:02 Endpoint: Reproduction Analysis: Parametric-Two Sample CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform 2:eta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run <0 0 N/A 19.3% Equal Variance t Two-Satnple Test Control vs Control Test Stat Critical MSO P-Value Decision(5%) ' Lab Control Conductivity Control 8.29 1.73 3.05 0.0000 Significant Effect ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Decislon(S%) Betw/een 1065.8 Error . 279.2 1065.8 15.51111 1 18 68,7 0.0000 Significant Effect Total 1345 1081.311 19 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%) Variances Variance Ratio F Distribution Shapiro-Wilk Normality 19.5 0.912 6,54 0.0001 0.0697 Unequal Variances Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff% 0 Lab Confrol 10 0 Conductivity Co 10 15.8 1.2 13.7 0.732 17.9 1,67 5 0 25 1.01 3 0.228 5.43 34.4% 0.0% 1.23 102.0% 92.4% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 3 0 Lab Control 25 3 20 2 19 2 18 1 17 1 0 16 13 0 0 0 13 12 .5 Graphics ts 20 I CL 3 15- ^1 Conc-% 0 Ld -O.S 0.0 O.S Rankits 000-089-163-4 CETIS™ v1,6.3revE Analyst; QA: Frestiwater Chronic Bioassay Client/Sample tD: Dudek & Associates/Upstream Test Number:_^CKi50l5= Daphnid Survival and Reproduction Datasheet Test Species: Odubia O. py/ex D. magna Start Date/Time: 4/23/2009 I M^"^ End Date/Time:-4/3W2e09__[i5ie <E>\\ Conc. LC Rep 8 9 10 Rand # 17 44 39 54 56 12 23 19 Tech o Daily Reproduction/ Survival Q "ST "TT •0 -XT o "cT IT o .;::;5;::. IT IT 8 ; J6 3E Total 16 QC \2. I MD Su 'iL. X5 tt it. :» i? Ifi Conc. CC Rep Rand 10 18 68 34 66 24 26 11 53 0 0 Jl. -6- o <5 "XT Daily Reproduction/ surviva IT o "XT T 0 0 Q •mm: O :2: mm^-'-' rto' 3 0 .•o •2-J2_ 175" Total 0 -li ± QC HO Conc. Rep Rand # 48 6.25% 10 70 16 21 62 50 38 33 0 73" _o_ o e G Dailv Reproduction/ Survival ^:-^W:Mm.imMm^imM: IT o -XT IT TT ..SL O Total TT 2s: QC Conc. 12.50% Rep Rand # 58 69 52 49 32 51 40 TT Daily Reproduction/ Survival •£2 O TT o TT 0 1_ 10 I 10 I 0 I O I ' 1— 1— ^ '—" ^ M.^n.i.c fnr ,..nh replicate Were blocked across concentrations at tegtinitiatlgn Rep:r^~n rn 3 L^^J^ I ^ L. ^. L I- IT 3: IE o 0 \0 Total Th.. TT o 4^ QC HO 11 3c> Board: Cup: , Verified By:l_Q^2Lj ^ _ ^, , Final Review: (^ S'/z-'/A ( QC Check: IT 5|uiD^ •- -zr^ ^ Naof/lus Env;ror;me/ita/ 5550 Morehouse Drive. Suite 150. San Diego, CA 92121. 2^1 ^6 Si 10 I I Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates/Upstream Test Number. Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 IM^"^ End Date/Time: 4/:gfe^ee^ O'M^ Conc. Rep Rand Dailv Reproduction/Survival Total : QC Conc. Rep # mi: m^ 3 msm:\' :;i:i;6i^i::i: \^m:l\] 1 31 0 6 0 0 0 % 0 U ' :: 25% 2 57 •"6 o 0 0 % —ys"7^— • 3 20 n o O b 0 l3 4 13 0 a \ \'3 \2. < 5 41 ft 0 0 4 0 O [Q- : —^— 6 45 —u 0 o O 7 o 0 7 9 — •— —--0 /^f 3 35 0 o b o 5 o g 30 d 6 0 O 1-5 0 •7 IH ; 10 55 6 o H \t> & 9 AKSi : Tpch: GFl Sl PF •Sl-5t flD Conc. Rep Rand Dailv Reproduction/ S urvival Total QC Conc. Rep # 1 3 m.Mmi m^m 7 8 1 4 0 "d O o rb 0 •7 17 50% 2 •37 0 0 O r o 'd -7 3 25 • 0 0 o h © H 4 46 c <3 0 p (. ^\ 5 42 0 O 0 0 k 6 65 0 G X) 0 V ?^ G S 7 61 0 r^ D o 8 43 0 —4=J o D 4-s 0 10 ; 9 5 0 o U a o VO 10 14 0 o —1>— (i \ "7 Conc. Rep Rand Dailv Reproduction/ Survival Total QC Conc. Rep # m.\m% m. 2 ;,^: :H.:;3:;.-.-mii^--/-m T ;•; 1 47 rs o /O 4' k o 1^ 100% 2 67 \J 0 0 0 0 0 (-> 9 • 3 27 0 o o 0 "7--0 o li 0 4 22 0 <0 0 D 0 -4— 5 59 0 o 0 o 0 (7 6 60 0 o o o 0 -7 14 7 36 0 o 0 0 1 O 8 63 0 0 o o 0 ~l )^ 9 29 0 o 0 •0 \ o 4 10 15 ^ 0 o -o •o. 2_ O 1 HP Neonates for each replicate were blocked across concentrations at test initiation I I I i '. 1 z I :: I -1 o Q Rep: Board: Cup: Verified By 5 ^1 1 10 ^3, Time Fed/Test Solution Renewed (day): (t-,i.i^^ s-i.tt' '0< ift>ii3:3Sr m)m3^(i)|i^o (2)_r3SO_(3)_Ji^L('»)JAi:i^(5)J3l5_(6)J]2c^ (.•tfjjzaasr- M^i^TT^ rie^dTM^ male; LIP =^ lost in progress: B = 4th brood (only the first 3 broods are included m total) QG Check:jT 5 IU ID'^ Nautilus Environmental 5550 Morehouse Drive. Suite 150. San Diego, CA 92121. Final Review: Freshwater Chronic Bioassay Client: Dudek Water Quality Measurements Test Species: C. dubia Sample ID: Upstream Test NO: OW^C^^ Start Date/Time: 4/23/2009 End Date/Time:-^jwoCTOS Analysts; Initial Final Sample Used (A, B, C); Comments: 0 ¥^ 1 MO 3 DP 4 s *^ MD MD //% SL HO SL MD A-A A A-A B; Animal Source/Date Received: ^t-'N-'^^'^* / Sample Log-in Numbers: A: 0^ QC Check: Naur/lus £nv/ron«,e„ra/ 5550 Morehouse Drivo, Suite 150. San Diogo, CA 92121. Animal Age at Initiation: C: Final Review Freshwater Chronic Bioassay Water Quality Measurements Client; Dudek Test Species: C. dubia Sample ID: Upstream Test No: Start Date/Time: 4/23/2009 / ^ ^ '-^ End Daterrime:-^a8ffl8e9 0*^^$ sll 1.01-P^ Concentration 25% Dav n 1 1 1 2 1 3 1 4 1 5 1 6 1 7 ^ y pH 7.^3 '2.0'=1 •^.'^^ A DO (ms/L) •7.8 S7 7 « , A Cond. ((imhos/cm) iW ii7l ^370 \21-3 IMMM A TomD (°C1 A—K—^-29 .-7 •2^7 A A pH 8,25 DO (mq/L) Temo (°C1 aH.s Concentration 50% Day 0 1 1 1 2 i 3 1 4 1 S 1 6 1 7 Initial pH 77q 7.'=IU 7.'',D i-^\ 7^<S. 7 at 69 Uiii / DO (mq/L) 0 7 at 69 Uiii / Cond. (nmhos/cm) a55o X370 • 2-3^7 XSOO 7 at 69 Uiii / Temp (°C) XM.7 « 25.7 25 7 7 at 69 Uiii / pH 6-2^ ??-Z7 tLLl DO (mq/L) 'g.o ^-^ TemD(°C) an.? . Z.M- Concentration 100% Day 0 1 1 ! 2 1 3 1 4 1 5 ,| 6 1 7 ;:sSj;:;SiSK:ss:m^^ pH in 7.75 l.E'\ 7,-50 7'«>9 DO (mq/L) i-^^ - a <? Cond. (iJmhos/cm) meg *f Temo (°C1 =2^-7 X5-^ PH <!S. M;2 DO (mq/L) a 3. Temp (°C) 5M.7 2«3 n 1 234567^ Analysts: Initial Final; Sample Used (A, B, C): w DF HO St ——— Analysts: Initial Final; Sample Used (A, B, C): HD Analysts: Initial Final; Sample Used (A, B, C): A 1 A IV ilii Comments: Animal Source/Date Received: Sample Log-in Numbers: A: QC ChecK; Nauiiius environmental 5550 MorohousQ Drive, Suite iSO. San Diego. CA 92121. Animal Age al Initiation: Final Review Site: Well #3 CETIS Sunimary Report Report Date: 03 Jun-09 10:42 (p 1 of 2) Ceriodaphnia 7-d Survival and Reproduction Test JO-O'»0O-,^..ii5C)/uau4-b091 Nautilus Environmental (CA) Test Run No: 00-8306-9490 Start Date: 23 Apr-09 15:15 Ending Date: 01 May-09 15:30 Duration: 8d Ih Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013 (2002) Species: Ceriodaphnia dubia Source: In-House Culture Analyst: Diluent: Brine: Age: Diluted Mineral Water (8:2) Nof Applicable <8hr Sample No: 13-1478-7140 Sample Date: 22 Apr-09 09:00 Receive Date: 23 Apr-09 10:30 Sample Age: 30h (5.8 °C) Code: 09-0325 Material: Groundwater Source: Dudek & Associates Station: Well 3 Client: Project: Dudek & Associates Comments: The lab control from the Downstream test (ID# 0904-S093) was shared and used for data analysis and comparisons Comparison Summary Analysis No Endpoint NOEL LOEL TOEL PMSD Method 05-3614-8194 7d Survival Rate 09-6180-0282 Reproduction 50 100 50 10O 70.7 70.7 N/A Fisher Exact/Bonferroni-Holm Test 44,1% Steel Many-One Rank Test Point Estimate Summary Analysis No Endpoint 00-2356-5395 7d Survival Rate Effect-% Conc-% 95% LCL 95% UCL Method 16-4681-0311 Reproduction 50 72.4 61.5 77.6 Test Acceptability Analysis No Endpoint Linear Interpolation (ICPIN) Attribute Control Resp Control Resp Control Resp Confrol Resp PMSD Test Stat Acceptabilty Limits Overiap Yes Decision Passes acceptability criteria 00-2356-5395 7d Sun/ival Rate 05-3614-8194 7d Survival Rate 09-6180-0282 Reproduction 16-4681-0311 Reproduction 09-6180-0282 Reproduction 1 1 15.8 15.8 0.441 0.8 - NL 0.8 - NL 15-NL 15-NL 0.13-0.47 Yes Yes Yes Yes 7d Survival Rate Summary Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Conc-% Control Type Count Mean 95% LCL 95% UCL Min 0 Conductivity C 10 0.6 0.407 0.793 0 0 Lab Control 10 1 1 1 1 6.25 10 1 1 1 1 12.5 10 0.8 0.643 0.957 0 25 10 0.9 0:782 1 0 50 10 0.7 0.52 0.88 0 100 10 0.4 0.207 0.593. 0 Max Reproduction Summary Conc-% Control Type Count Std Err Std Dev CV% Diff% 0.0943 0.516 86.1% 0.0% 0 0 0.0% -66.7% 0 0 0,0% -66.7% 0.077 0.422 52.7% -33.3% 0.0577 0,316 35.1% -50.0% 0,0882 0.483 69.0% -16.7% 0.0943 0.516 129.0% 33.3% Mean 0 0 6,25 12.5 25 50 100 Conductivity C Lab Control 95% LCL 95% UCL Min 10 10 10 10 10 10 10 Max 3.4 , 15.8 21.5 20.7 19,7 16.4 1.5 1.76 13.8 19.2 16.9 16.6 14.3 0.516 5.04 17.8 23.8 24.5 22.8 18.5 2.48 std Err Std Dev CV% 0 5 11 0 0 4 0 Diff% 13 25 30 30 28 24 8 0.804 0.992 1.14 1.86 1.5 1.02 0.481 4.4 5.43 6.26 10.2 8.23 5.56 • 2.64 129.0% 34.4% 29.1% 49.1% 41.8% 33.9% 176.0% 0.0% -365.0% -532.0% -509.0% -479.0% -382.0% 55,9% 000-089-153-3 CETIS™ vl ,6.3revE Analyst: QA: CETIS Summary Report Report Date: 03 Jun-09,10:42 (p 2 of 2) Ceriodaphnia 7-d Survival and Reproduction Test > Nautilus Environmental (CA) 7d Sun/ival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 1 0 0 1 0 1 1 1 0 1 0 Lab Control 1 1 1 1 1 .) 1 •] 6.25 1 1 1 1 1 t 1 1 , 1 12.5 0 1 1 1 1 1 1 1 1 0 25 1 1 1 1 1 1 1 0 1 1 50 1 1 0 1 1 1 1 0 1 0 100 1 1 0 0 0 0 1 0 0 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Reps Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 0 0 0 8 0 4 13 0 4 5 0 Lab'Control 19 13 16 25 12 17 6 18 20 13 6.25 11 14 30 18 22 19 20 26 28 27 12.5 0 22 29 20 19 26 26 30 29 6 25 22 22 24 19 15 15 26 0 28 26 50 18 24 4 17 15 16 21 21 16 12 100 2 8 1 0 0 0 0 4 0 0 000-089-163-3 CETIS™ v1.6.3revE Analyst:, QA: CETiS Analytical Report Report Date: Link/Link Code: 03 Jun-09 10:18 (pi of 1) 1 ST8483-2286/0904-S091 Ceriodaphnia 7-d Sun/lval and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 05-3614-8194 03 Jun-09 10:16 Endpoint: 7d Survival Rate Analysis: STP 2x2 Contingency Tables CETIS Version: Official Results: CETlSvl.6.3 Yes Data Transform Zeta Untransformed Alt Hyp Monte Cario NOEL LOEL TOEL C>T TU PMSD Not Run 50 100 70.7 N/A Fisher Exact/Bonferronl-Holm Test Control vs Conc-% Lab Control 6.25 Test Stat P-Value Oecision(0.05) 12.5 .25 50 100 1 0.237 0.5 0.105 0.00542 1 0.711 1 0.421 0,0271 Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Data Summary Conc-% Control Type No-Resp Resp ""o 0 •2 1 3 6 Total 10 10 10 10 10 10 6.25 12.5 25 • 50 100 10 10 8 9 7 4 7d Survival Rate Detail Conc-% Control Type Repl Rep 2 Rep 3 0 6.25 12.5 25 50 100 Lab Control Rep 4 Reps Rep 6 Rep 7 Rep 8 Rep 9 Rep io Graphics 0 1 0 0 I.On 0.9- O.S- 0.7- 0.6- O.S- 0.4. 0.3-) 0.2- 0.1 0.0 6.25 12.5 25 Conc-% 000-089-163-4 CETIS™ v1.6.3revE Analyst: If CETIS Analytical Report Report Oate: Link/Link Code: 03 Jun-09 10:41 (p 1 of 1) 15-8483-2286/0904-S091 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 00-2356-5395 Endpoint: Analyzed: 03 Jun-0910:40 Analysis; Nautilus Environmental (CA) 7d Sun/ival Rate Trimmed Spearman-Karber CETIS Version: Official Results: CETISvl.6.3 Yes Spearman-Kariier Estimates Threshold Option Threshold Trim Mu Control Threshold Sigma 40.00% 1.9 EC/LC50 9S%LCL 95% UCL 0.114 79.4 46.9 134 7d Survival Rate Summary Conc-% Control Type Count Mean Min 0 Lab Control 10 1 , 1 6.25 10 1 1 12,5 10 0.8 0 25 10 0.9 0 50 10' 0.7 0 100 10 0.4 0 Calculated Variate(A/B) Max Std Err Std Dev CV% Diff% A B 0, 0 0,0% 0.0% 10 10 0 0 0.0% 0.0% 10 10 0.077 0.422 52.7% 20,0% 8 io 0.0577 0.316 35.1% 10.0% 9 10 0.0882 0.483 69.0% 30.0% 7 10 0.0943 0.516 129.0% 60.0% 4 10 7d Survival Rate Detail Conc-% Control Type Rep 1 0 6.25 12,5 25 50 100 Lab Conlrol Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 1 1 0 1 0 0 Graphics i.o» • 0.9- 0,8- S 0.7- r 0.6- 1 0.5- =1 0.3- 0.2- 0.1 0.0 10 60 Conc-Vo 000-089-163-4 CETIS™ v1.6.3revE Analyst;, U OA: A CETIS Analytical Report Report Date: 03 Jun-09 10:18 (pi of 2) Ceriodaphnia 7-d Survival and Reproduction Test 15-848j-2Ze6/0904-S091 Nautilus Environmental (CA) Analysis No: 09-6180-0282 Analyzed: 03 Jun-09 10:17 Endpoint: Reproduction Analysis: Nonparametric-Control vs Treatments CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Cario NOEL LOEL TOEL TU PMSD Rank C > T. Not Run 50 100 70.7 2 44.1% Steel Many-One Rank Test Control vs Conc-% Lab Control a25 Test Stat Critical Ties P-Value Decision(5%) 12.5 25 50 100' 7^ 2 1.0000 Non-Significant Effect 75 1 0.9990 Non-Significant EIFfect •"^^ 75 4 0.9000 Non-Significant Effect _ff 75 0 0.0005 Significant Effect ANOVA Table Source Sum Squares Between Error 2764.533 2499.2 Total Mean Square 552.9067 46.28148 DF 5 64 FStat 11.9 P-Value Decision(5%) 0.0000 Significant Effect 5263.733 599.1882 59 ANOVA Assumptions Attribute Test Variances Bartlett Equality of Variance 14.9 Distribution Shapiro-Wilk Normality 0.908 Test Stat Critical P-Value Decision(1%) 15.1 0.0106 0.0003 Equal Variances Non-normal Distribution Reproduction Summary Conc-% Control Type Count Mean 0 6.25 12.5 25 50 100 95% LCL 95% UCL Min Lab Control Max 10 10 10 10 10 10 15.8 21.5 20.7 19.7 16.4 1.5 13.7 19.1 16.8 16.6 14.3 0.498 17.9 23.9 24.6 22.8 18.5 2.5 Std Err Std Dev CV% 5 11 0 0 4 0 Diff/o 25 30 30 28 24 8 1.01 1.16 1.89 1,53 1:03 0.489 5.43 6.26 10.2 8.23 5.66 2.64 34,4% 29.1% 49.1% 41.8% 33.9% 176.0% 0.0% -36.1% -31,0% -24,7% -3.8% 90.5% Rank Transformed Summary Conc-% Control Type Count 0 6.25 12,5 25 50 100 Lab Control 10 10 10 10 10 10 Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff/o 27.6 23.5 31.6 13 48 1.99 10,7 38.9% 0.0% 40.4 34.7 46.1 16 59.5 2,77 14.9 36.9% -46,6% 41.1 33.9 48.3 4.5 59.5 3.5 18.9 45.9% -49.2% 37.6 .31.4 43.8 4.5 55.5 3.01 16.2 43,1% -36,5% 29.1 25 33.2 11.5 46.5 2.02 10.9 37.4% -5.63% 7.25 5.78 8.72 4.5 15 0.717 3,85 53.2% 73.7% 000-089-163-4 CETIS™ v1.6.3revE Analyst: QA:_/6 CETIS Analytical Report Report Date: Link/Link Code: 03 Jun-09 10:18 (p 2 of 2) 15-8483-2286/0904-S091 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 09-6180-0282 Analyzed: 03 Jun-09 10:17 Endpoint- Reproduction Analysis: Nonparametric-Control vs Treatments CETIS Version: CETISvl.6,3 Official Results: Yes Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 25 20 19 18 17 16 13 13 12 ' 5 6.25 30 28 27 26 22 20 19 16 14 11 12.5 30 29 29 26 26 22 20 19 6 0 25 28 26 26 24 22 22 19 15 15 0 50 24 . 21. 21 18 17 16 16 15 12 4 100 8 4 2 1 0 0 0 0 0 0 Graphics 1 II 30- 20- 10 0 -10- -20- -30- 12.5 25 Conc-°/p -40- -2.5 -2.0 -1.5 -1.0 -O.S 0.0 O.S Ranicits 1,0 1.S 2.0 2.5 000-089-163-4 CETIS™ v1.6.3revE Analyst: OA: L CETIS Analytical Report Report Date: Link/Link Code: 03 Jun-09 10:41 (p 1 of 1) 15-8483-2286/0904-S091 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 16-4681-0311 Endpoint: Reproduction CETIS Version: CETlSvl.6.3 Analyzed: 03 Jun-09 10:41 Analysis: Linear Interpolation (ICPIN) Official Results: Yes Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 95% CL Method Linear Linear 7055475 280 Yes Two-Point Interpolation Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 72.4 61.5 77.6 Reproduction Summary Calculated Varlate Conc-% Control type Count Mean Min Max Std Err Std Dev CV% Diff% 0 Lab Control 10 15.8 5 25 0.992 5.43 34.4% 0.0% 6.25 10 21,5 11 30 1.14 6.26 29.1% -36.1% 12.5 10 20.7 0 30 1.86 10.2 49.1% -31.0% 25 10 19.7 0 23 1.5 8.23 41.8% -24.7% 50 10 16,4 4 24 1.02 5.56 33.9% -3.8% 100 10 1.5 0 8 0.481 2.64 176.0% 90.5% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 19 13 16 25 12 17 5 18 20 13 6.25 11 14 30 18 22 19 20 26 28 27 12.5 0 22 29 20 19 26 26 30 29 6 25 22 22 24 19 15 15 26 0 28 26 50 18 24 4 17 15 16 21 21 16 12 100 2 8 1 0 0 0 0 4 0 0 Graphics 25- 20-...» c O 1 •fi 15- 3 "O 5 o. S 10- 5- • 0 1 1— 1 20 40 1 60 80 100 120 Conc-% 000-089-163-4 CETIS™ v1.6.3revE Analyst: QA: CETIS, Report Date: 03 Jun-09 10:20 (p 1 of 1) 15-8483-2286/0904-S091 Ceriodaphnia 7-d Survival and Reproduction Test / Nautilus Environmental (CA) Analysis No: 08-5985-9782 Endpoint: 7d Survival Rate CETIS Version: CETlSvl.6.3 Analyzed: 03 Jun-09 10:17 Analysis: Single 2x2 Contingency Table OfTicial Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run <0 0 N/A N/A Fisher Exact Test Control vs Control Test Stat P-Value Decision(0.05) Lab Control Conductivity Control 0.0433 0.0433 Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 Conductivity Co 6 4 10 0 Lab Control 10 0 10 7d Survival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 1 1 1 1 1 1 0 0 0 0 0 Lab Controi 1 1 1 1 1 1 1 1 1 1 Graphics 1.0^ 0.9- 0.8- 0.7- 0.6- 0.5- 0.4- 0.3 0.2- 0.1- 0.0 0 CC Conc-% LC 000-089-163-4 CETIS™ v1.6.3revE Analyst: : br QA: I CETIS Analytical Report /! i /, i c /> i \ • ^ ( Z.cd> LVCVTOV VS. 6g>/w>'CTtui Report Date: I Link/Unk Code: 03 Jun-09 10:20 (p 1 of 1) 15-8483-2286/0904-S091 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 18-6723-7215 Analyzed: 03 Jun-09 10:17 Endpoint: Reproduction Analysis: Parametric-Two Sample CETIS Version: Official Results: CETlSvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Cario NOEL LOEL TOEL TU PMSD Untransformed OT Not Run <0 0 N/A 24.3% Equal Variance t Two-Sample Test Control vs Control Test Stat Critical MSD P-Value Decislon(S%) Lab Control Conductivity Control 5.61 1.73 3.83 0.0000 Significant Effect. ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Decislon(5%) Between 768.8 Error 440 768.8 24.44444 1 18 31.5 0,0000 Significant Effect Total 1208.8 793.2444 19 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%) Variances Variance Ratio F Distribution Shapiro-Wilk Nonnality 1.52 0.934 6.54 0.5410 0.1830 Equal Variances Normal Distribution Reproduction Summary Conc-% Controt Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff% 0 Lab Control 10 0 Conductivity Co 10 15.8 3.4 13.7 1.73 17.9 5,07 5 0 25 1.01 13 0.817 5.43 34.4% 0,0% 4,4 129.0% 78.5% Reproduction Detail Conc-% Control Type Repl Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 13 0 Lab Control 25 8 20 5 19 4 18 4 17 0 , 0 15 13 0 0 0 13 12 5 Graphics 30- B 20^ 1 St; c c Cl re 0 CC Conc-% 0 -2.0 -1.5 ^).5 0.0 O.S Rankits 000-089-163-4 CETIS™ v1,6.3revE Analyst:. QA: rresnwater Chronic Bioassay Client/Sample ID: Dudek & Associates/Well 3 Test Number: 0(0^-50^1 Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 iSTlS End DatelTme-.'^immm >ieBc&-^ Conc. Rep Rand Daily Reproduction/ Survival Total "'7 1 h : QC Conc. Rep # m^im-^ A \:mmi 6 ::;;;;^i^;;^:^; 8 Total "'7 1 h : QC tc 1 17 C) o •o a "7 tc 2 6 C) - o o 0 0 o •a 1- 3 44 -0 0 o M , 4 39 - n o H o 0 5 54 0 0 o ^-o -7 6 56 0 • D (9 ,^ o 0 r^ ' iO 7 12 0 0 O 2. Lii m 8 23 o V 9 U W~ 9 19 c 0 a % A/0 • y-^ 0— 10 64 0 u o o q o Tech: SL 1 ^^ JO ir_ Conc. Rep Rand Daily Reproduction/Survival QC Conc. Rep # ;:i:i:;1:;;;:; ::;::::;;3:;-:: ; :;:^::;4:n: 7 8 - Total QC 1 8 c 0 ~ —,—J... '6' o o O O 0 2 18 (S 0 OICKJ — _.. —•. — I: 3 68 . D o Ol(k. -— . 4 34 0 O o 0 o (3) — : —' 5 66 (b o o O MO 6 24 c 0 o o O 1 7 26 0 o c> . 0 (3' 13 8 11 0 r> V n O \— - o o 0 9 53 0 9. V <=l 0 0 O i\ klo I 10 28 0 u K) D f) fl n i b Conc. Rep Rand Daily Reproduction/ Survival Total QC Conc. Rep # 1 2 3 m{M^ 5 mm(m 8 Total QC 1 48 O o £> O •3 o 9i 2 70 0 0 u 0 o 5 o C) m .—fit „ 3 2 Cl Ci U 0 <s / , O 4 16 e o 0 o o «r o iO I'S 5 21 (i o 0 ^ o o -—•J. V 6 62 a o o <o 0 7 50 a D o 2. o to 0 . :—* 20 8 38 C) O -o 0 7 \s o 0.(0 9 33 0 o 5' 0 10 3 0 u n <^ 16 o ' Ci . Conc. Rep Rand Daily Reproduction/ S urvival Total QC Conc. Rep # 1 '\m2::m: i-mi^A :;;^::;4::;Hi; M\]^m mimm 7 Total QC 1 58 6 O/ci — • — 2 69 b o o O o o 3 52 0 o e=i 4 o IO o 4 49 0 u o o 7 o V5> • 5 7 f) o o o a .1 6 • 32 . 0 o o . H a o 7 51 0 o o "3-0 is StU 8 40 0 O o (-7 o 9 1 a ^) o o • V:L/ : ill 10 10 0 u y -^ZS—1 L LP/. Neonates i or each replicate were blocked across concentrations at tfi.qt i nitiatior —'-v/zaa Rep: 1 2 3 4 5 6 7 8 9 10 Board; An <?;^ 9^ Cup: 7 SS Verified By: ^^fcj Time Fed/Test Solution Renewed (day): (O)J^J^(I) <0^0 (2)_jig_ (3) 12-26 (4) i40O (5) IZOS (6) |(50 (7)_V^ ^ - dead; M = male; LIP = lost in progress: B = 4th brood (only the fjrst 3 broods arje inciuded in total) 6) QC Check: ,]> 5(U|D^ Final Review: ^ ^/>-'?Al A/ouri/us Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & AssociatesA/Veli 3 Test Number: CTOH-$0^ Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 IS', (ST End Date/Time: 4/30/2009 mo Conc. Rep Rand Daily Reproduction/ Survival i QC # ::;i:;^?li;:ii! 2 m--m^:-i : m^m^. \ iii: ;:;::::;6:^:r: i:;;i;i^i::i 8 - Total i QC 1 31 6 o o 2-7 o \S 2 57 0 \ o -7 0 'iM 3 20 0 o o o 7 0 4 13 C> a o c? o 7 o — 1 5 41 0 O o 2. o o 6 45 0 o V 0 H o u —1 7 9 0 o o 3 o o I'D" 8 35 0 o o OlcL -— 9 30 0 6 7 o o 10 55 lb €> V V 9r \^ '• 6 Tech: Hi? SL SL SL. m Conc. Rep Rand Daily Reproduction/Survival Total i QC Rep # '\\mm mmmmmmmm mmm ;iiiii:&;H;:i 7 8 Total i QC 1 4 e <o 0 S c3 2 37 D O M o O it-"^i :— 3 25 0 —Y'v <D u OlcL — H/J 4 46 0 O o O <S 5 42 o V c o <? IS ^ 6 65 0 O O o •2-' s 9 IU 7 61 0 0 0 O 5 0 l"^ —I . 8 43 -a a o 0 \o A/0 9 10 5 14 6 •0 o c? 6 S O ^-C/ 7 —- Conc. Rep Rand Daily Reproduction/ Survival Total : QC Rep # m-mm ii;ivi2;;:i:; •""^sci^; :i:i:i;S;:;:: ••m^'y:. :i:i:;i?;::;:. ;;.;;;s;;.;; Total : QC 1 47 0 0 © 0 0 i X 2 67 (h 0 u 0 0 • S 3 27 c Q 0 0 0 0 I 4 22 f. 0 T) -ofA 5 59 0 —f—t 0 —-— O/A 6 60 -— — —^/ F-O/A 7 36 0 0 0 0 -—— 8 63 . 0 a 0 0 ) 0 u SWIM 9 10 29 15 0 t)/el 0 u — ScI Neor lates for eac h repliCc te were blockec 1 across concer 1 trations at test 0/' nitiation ._ Rep: 1 2 3 4 5 6 7 8 9 10 board: =5-"2-'I Cup; > J V < IXs 56 veritied By: Time Fed/Test Solution Renewed (day)- *y (0)J^(1)l0liil_(2)Jl6O_(3,j2^(4)aH0L(5) fe^ (6)JI5^(7) C^^^ ""'""^ ' = dead^JVl^n^ UP = lost in progress: B = 4th hrn-;;;r^v thTTi^iTJbrQ-^^h^rincluSt^tan QC Check: DF Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121 Final Review: Freshwater Chronic Bioassay Client: Dudek Sample ID: Well 3 Test No: Water Quality Measurements Test Species: C- dubia Start Date/Time: 4/23/2009 t ^ 1^ End Date/Time: Concentration Lab Control Day 0|1|2|3|4|5|6|7 , Initial pH «.2-4-l.<\\ ?.i^ -3. 15' • DO (mg/L) 7-7 - ?.3 7-7. to -3. 15' • Cond. (pmhos/cm) aoi -3. 15' • Tomp (°C) 2 5.<=l as-a 25 (b -3. 15' • Rnal PH ?,l3 e. It^ DO (mg/L) 7.9 &^ •So Temp(°C) 3M.(C2 Concentration Conductivity Control Day 0 1 1 1 2 1 3 1 4 1 5 1 6' 1 7 • - Initial - pH 1.%1 7 7^4 .T 5".-7 DO (mq/L) 7^ •7-t/' 7-^ .T 5".-7 Cond. (pmhos/cm) <5b70 4XSO 5*470 .T 5".-7 Temp ("C) 2.5.1 aLV7 .T 5".-7 pH %.\^ 7^ DO (mg/L) 'STL Temp (°C) 2-4-3 an .5 Concentration 6.25% Day 0|ll2|3|4|5|6|7 Initial pH 7.72 7'7q 7.lO 7-27 •a CM •7.7 DO (mg/L) 7-7 7. IV '?.<?, 1.1 11 •a CM •7.7 Cond. (pmhos/cm) 67-4 MS2. 693 •a CM •7.7 Temp (°C) 0:0'* 0^ 24-7 •2 too •a CM •7.7 pH iiiliffi i^. ai •6.30 ^./^ DO (mg/L) iiiliffi •7.9 Temp CC) iiiliffi ^4.7 '2M'2> 1 Concentration Day 12.6% _EH_ DO (mq/L) Cond. (pmhos/cm) Temp CC) .0 7.U7 7-SS 25-fe Initial I 7.g5 A7r 7-71 7d_ pH DO (mg/L) Temp CC) ^7g^ 7,9 34-g 33^ 7:5il_ 1067 2fo,0 7.<Jf^ •S 0 ^gH7 ^4..<^ •n ZM.3 Analysts; Initial: ^6 HP Final: SL Sl- Sample Used (A, B, C): A Comments: Animal Source/Date I I Received: InViwvJL^,-^-gt*-^. tvjD^^^^ta/j^ Sample Log-in Numbers; QC Check; \fr 5|U)ica Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Animal Age at Initiation: ^:^4V~\i. C: Final Review Freshwater Chronic Bioassay Client: Dudek Water Quality Measurements Test Species: C. dubia Sample ID: Well 3 Test No: start Date/Time: 4/23/2009 [ End Date/Time: Mionooo /0>3<7 Concentration Day 25% pH DO (mg/L) Cond. (pmhos/cm) TEmpt'C). 7-3'2-7.q4 -7- 4 H.L Initial T.jjl] 7d_ 141 M 7-55 7'\^0 7 5 7(^^ JJH. DO (mq/L) Temp CC) .^47 7,? Finals Z1,7 S4 W) ff Concentration 50% 'S7 Day 0|lf2|3|4lsi6l7 'S7 'S7 pH 7,\S 72.^ 7 30 7.-2.4 7.31 7 -B-a 7.Hl 'S7 DO (mq/L) 77?. 17 87 ?^1 7.Hl 'S7 Cond. (pmhos/cm) ( 30C0 3030 7.Hl 'S7 Temp CO c '2.U>.C> 2^.5 14.^ =250 7.Hl 'S7 'S7 pH S^.'^7 5^' 0L| 'S7 DO (mq/L) 7-? ^-^ «.( 'S7 Temp (°C) >?f--7 2.M-3 Concentration . 100% Day 0 1 1 1 2 ! 3 1 4 1 5 1 G 7 PH i 7 IS DO (mq/L) 7.1 7-2 8 ^ %^ Cond. (pmhos/cm) 5010 4440 H''t70 f?4<S0 Temp CC) XM.b 7 "5.3 PH <?r.3'7 DO (mq/L) 7, <) «\ Temp (°C) X\2> &5 iiiii SL HO St. Analysts: Initial: Final: Sample Used (A, B, C): • Comments: Animal Source/Dale Received: {if\t^\OVi''~ 1 A/A Sample Log-in Numbers: A: " (TT^^^T) B: QC Check: DF S|(o.lO<^ Nautilus Environmental 5550 /More/ioi/so Dr/ve, Su/ie J50. San Diego, CA 92131. Animal Age at Initiation: 1 C: Final Review: Site: Weil #1 CETiS Summary Report Report Date: Link/Link Code: 29 May-09 16:24 (p 1 of 2) 11-8639-9634/0904-S090 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Test Run No: 08-9587-0877 Test Type: Reproduction-Survival (7d) Analyst: start Date: 23 Apr-09 15:30 Protocol: EP/V821/R-02-013 (2002) Diluent: Diluted Mineral Water (8:2) Ending Date: 30 Apr-09 16:05 Species: Ceriodaphnia dubia Brine: . Not Applicabie Duration: 7d Ih Source: In-House Culture Age: <8hr Sample No: 00-8142-8426 Code: 09-0324 Client: Dudek & Associates Sample Date: 22 Apr-09 11:00 Material: Groundwater Project: Receive Date: 23 Apr-09 10:30 Source; Dudek & Associates Sample Age: 28h (4.9 °C) Station: Well 1 Comments: The lab control from the Downstream test (ID# 0904-S093) was shared and used for data analysis and comparisons. Comparison Summary Analysis No Endpoint NOEL LOEL TOEL PMSD Method 20-9667-9981 7d Survival Rate 100 > 100 N/A N/A Fisher Exact/Bonferroni-Holm Test 16-6446-2124 Reproduction 100 >100 N/A 40.5% Bonferroni Adj t Test Test Acceptability Analysis No Endpoint Attribute Test Stat Acceptabilty Limits Overlap Decision 20-9667-9981 7d Survival Rate Control Resp 1 0.8-NL Yes Passes acceptability criteria 16-6446-2124 Reproduction Control Resp 15.8 15-NL Yes Passes acceptability criteria 16-6446-2124 Reproduction PMSD 0.405 0.13-0.47 Yes Passes acceptability criteria 7d Survival Rate Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Dlff% 0 Conductivity C 10 1 1 1 1 1 0 0 0.0% 0,0% 0 LabControl 10 1 1 1 1 1 0 0 0.0% 0,0% 6.25 9 1 1 1 1 1 0 0 0.0% 0.0% 12.5 10 1 1 1 1 . 1 0 0 0.0% 0.0% 25 10 1 1 1 1 1 0 0 0,0% 0,0% 50 10 1 1 1 1 1 0 0 0.0% 0,0% 100 10 1 1 1 1 1 0 0 0,0% 0.0% Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev CV% Diff% 0 Conductivity C 10 16.6 14.3 18.9 6 23 1.1 6.04 36,4% 0.0% 0 LabControl 10 15,8 13,8 17.8 5 25 0.992 5.43 34.4% 4.82% 6.25 9 18.6 16.6 20.5 8 25 0.971 5.32 28.7% -11.8% 12.5 10 17 13.8 20.2 6 28 1.55 8.47 49.8% -2,41% ,25 10 19.7 17.7 21.7 12 26 0.985 6.4 27.4% -18.7% 50 10 21,2 18.9 23.5 11 27 1.12 6.12 28.9% -27,7% 100 10 10.7 9.21-12.2 6 17 0.731 4 37.4% 35.5% 000-089-163-3 CETIS™ v1.6.3revE Analyst: QA: CETIS Summary Report Report Date: Link/Link Code: 29 May-09 16:24 (p 2 oT . 11-B639-9634/0904-S090 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 1 ,1 1 1 1 1 1 0 Lab ContnDl 1 1 1 1 1 1 1 1 6.25 1 1 1 1 1 1 1 1 12,5 1 1 1 1 1 1 1 1 1 25 1 1 1 1 1 1 1 1 50 1 1 1 1 1 1 1 1 1 100 1 1 1 ' ^ 1 ^ 1 ^ Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Reps Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 23 "8 6 11 21 20 18 22 18 19 0 LabControl 19 13 16 25 12 17 5 18 20 13 6.25 23 8 17 16 25 19 24 20 15 12.5 28 10 6 11 23 23 11 24 8 26 25 26 22 12 13 23 16 26 23 14 22 50 25 27 12 11 16 26 24 24 27 20 100 7 8 9 12 •15 17 9 16 6 8 000-089-163-3 CETIS™ v1.6.3revE Analyst: QA^ CETiS Summary Report. Report Date: Link/Link Code: 29 May-09 16:24 (p 2 of 2) 11-8639-9634/0904-S090 Ceriodaphnia ird Survival and Reproduction fest Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% Control Type Rep 1 , Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 1 1 1 ,1..-1 1 1 1 1 1 O' Lab Contnsl • 1 1 .-1 • il • 1 ' 1 1 1 1 6.25 ,' 1 , 1 • • • 1.. • • • .1 • -•• V 1 1 1 1 12.5 1 1 ,1 1 1 1 1 1 1 1 25 1 1 . .1 .... - •., :. 1 •1 , 1 1 1 1 50 1 ••• • • ••yV:-1 • 1 il- ••" 1 • 1 1 1 1 100 1 1 1 • Y.;. .1' 1 ' '1 •I 1 1 Reproduction Detail Conc-% Coiitrol Type Rep 1 Rep 2 Rep 3 • Rep 4 Rep Sr i Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity Co 23 6 • M " 21 20 18 22 18 19 0 Lab Control 19 13 16 25 12 17 • 5 18 20 13 6.25 23 - •• 8 .7 ..... . ... !17 • • 16 • -25 '19 24 20 15 12.5 28 10 6- • ' 11 23 • 23 11 24 8 26 25 26 22 12 13-23 . 16 26 23 14 22 50 25 •--•'(2 •!t1 ' , 26 -i -24 .: • 24..: ..: .. 27 : 20-... 100 7 8 9 12 15 17 9 16 6 8 000-089-163-3 CETIS™ v1.6.3revE Analyst CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 16:24 (p 1 of 1) 11-8639-9634/0904-S090 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 20-9667-9981 Endpoint: 7d Sun/ival Rate Analyzed: 29 May-09 16:22 Analysis: STP 2x2 Contingency Tables CETIS Version: Official Results: CETlSvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% Test Stat P-Value Declsion(0.05) Lab Control 6.25 1 1 Non-Significant Effect 12.5 1 1 Non-Significant Effect 25 11 Non-Significant Effect 50 11 Non-Significant Effect 100 1 1 Non-Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 Lab Control 10 0 10 6.25 9 0 9 12.5 ,10 0 10 25 10 0 10 50 10 0 10 100 10 0 10 . 7d Survival Rate Detail Conc-% Control Type Rep1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control .1 .1 . . . ^ . 1... ; .; .. 1 . 1 . • .1 1 1 1 6.25 •.r' • . 1 •. . • 1 ST. ' : x-:\ 1 1 1 1 12.5 , 1 1 • 1 . . ^ 1 . /I: 1. . 1 1 1 1 25 1 i 1 ..i ,1 1 . 1. • .: 1 1 1 1 1 50 ,ii 1 ..• 1 1 . 1 1 . 1 1 1 1 100 1 1 1 • 1 •.. . 1: . 1- ... . 1 1 1 1 Graphics 1.0- 0.9- £ 0.8- ro Of 0.7-1 i o;6- cn 0.5- 0.4- 0.3- o.^ O.l- 0.0 12.5 25 Conc-% 000-089-163-4 CETIS™ v1,6.3revE Analyst:. VE_ QA:^ CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 16:24 (p 1 of 2) 11^639-9534/0904-8090 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysts No: 16-6446-2124 Endpoint: Reproduction CETIS Version: CETlSvl.6.3 Analyzed: 29 May-09 16:22 Analysis: Parametrio-Muttiple Comparison Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run 100 >100 N/A 1 40.5% Bonferroni Adj t Test Control VS Conc-% Test Stat Critical MSD P-Value Decision(S%) Lab Control 6.25 -1.01 2,4 6.57 1.0000 Non-Significant Effect 12.5 -0.45 2.4 6.39 1.0000 Non-Significant Effect 25 -1.46 2.4 6.39 1.0000 Non-Significant Effect 50 -2.03 2.4 6.39 1.0000 Non-Significant Effect 100 1.91 2.4 6.39 0.1530 Non-Significant Effect ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Declsion(S%) Between 681.293 136.2586 5 3,84 0.0049 Significant Effect Error 1881.622 35.50231 53 Total 2562.915 171.7609 58 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%>) Variances Bartlett Equality of Variance 5.37 15.1 0.3730 Equal Variances Distribution Shapiro-Wilk Normality 0.962 0.(3649 Norrial Distribution Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min Max Std Err Std Dev GV% Diff% 0 Lab Control 10 15.8 13.7 17.9 5 25 1.01 5.43 34,4% 0.0% 6.25 9 18.6 16.5 20.6 8 25 0.987 5.32 28.7% -17.4% 12.5 10 17 13.8 20.2 6 28 1.67 8.47 49,8% -7.59% 25 10 19.7 17.6 21.8 12 26 1 5.4 27.4% -24.7% 50 10 21.2 18.9 23.5 11 27 1.14 6.12 28.9% -34.2% 100 10 10.7 9.18 12.2 6 17 0.743 4 37.4% 32.3% 000-089-163-4 CETIS™ v1.6.3revE Analyst: QA: CETIS Analytical Report Report Date: Link/Link Code: 29 May-09 16:24 (p 2 of 2) 11-8639-9634/0904-S090 -Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 16-6446-2124 Endpoint: Reproduction Analyzed: 29 May-09 16:22 . Analysis: Parametric-Multiple Comparison CETIS Version: CETlSvl.6.3 Official Results: Yes Reproduction Detail Conc-% Controt Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 0 Lab Control 25 20 19 18 17 16 13 13 12 5 6.25 25 24 23 20 19 17 - 16 15 8 12.5 28 26 24 23 23 11 11 10 8 6 25 26 26 23 23 22 22 16 14 13 12 50 27 27 26 25 24 24 20 16 12 11 100 17 16 15 12 9 9 8 8 7 6 Graphics 30- o B 20- IS li c c SI Rejsmriii 12.5 25 Conc-% -IS--T 1 1- -2.5 -2.0 -1.5 -1.0 -0.5 O.Q 0.5 1.0 1.5 2,0 2.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst : W QA: Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates/ Well 1 Test Number: Q40Mr5Q^O Daphnid Survival and Reproduction Datasheet Test Species; C. dubia D. pulex D. magna start Date/Time: 4/23/2009 ^^$30 End Date/Time: 4/30/2009 Upg" Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # ii;i;iiiii:i:i;i mmm mM^m 4 . 5 . 6 7"" 8 Total QC LC 1 17 d' t> H "D « b • l'5^ LC 2 6 o o o O XJ r 3 44 0 o 3 TH 4 39 0 o 1-O P, ID 5 54 0 a 0 o \o l'l 6 56 (5 o o 2-0 H IO 7 12 0 o •o ^2-o Lie - L 8 23 6 o 0 3 o & \% 9 19 0 o D •2-C I to 64 c> o •I Tech: ^0 MD SL i?'.8' Avevo Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # iiiiii:;?;'-;^ ::.^;^;:3a:^ 4 5 7 8 Total QC CCL 1 8 o o o o % to CCL 2 18 0 ci o • 0 2. (a o % 3 68 n 0 0 o O c o to 4 34 o O 0 o f o "7 l\ 5 66 0 0 Q P> o h \f 51 6 24 b o O •A-£> <? 7 26 O 0 4 1^ 8 11 t O O. i ? ;aP 9 53 0 o O O IS 10 28 0 0 0 o 3 9 n Conc. Rep Rand # Daily Reproduction/ Survival Total QC Conc. Rep Rand # 1 \' 2 :>mi:m i;i:Hi4;i;i:i: 5 iiiii:;6;i;-i :Hi;i;i^;iii;; iiiii;iSiiiv Total QC 1 48 o o O ta 2 70 ft o o O 5 3 2 0 o '0 4 O - Jfc P 4 16 0 o % o O c= i{ 17 5 21 . • 0 0 o D o / Ito 6 62 G 0 o o <^ — 7 50 r> o •a 0' H 8 38 © •0 Q T 514 9 33 0 o 0 D t» •1 ll 20 10 3 0 0 0 4 O H 7 7 k^L. Conc. Rep Rand Daily Reproduction/ Survival Total QC Conc. Rep # •m:Tm ::i:i:i2;i:::i i:::i>:3::iii; 5 mi}m\\ iHiiiiai:;-Total QC 1 58 o O o 1 '1 2 69 o o M o b o ID 3 52 0 o o o 0 o Iff 4 49 CJ o 4 0 o 11 5 7 0 o '(^ o 7 to 6 32 0 6) •0 <J \3 a. ^ 7 51 (S O • 0 4 0 7-o II 8 40 o o € " 7 t^ aH 9 1 0 \) o V 7^ s 10 10 0 o () 4 0 -' 5r Neonates for each replicate were blocked across concentrations at test initiation Rep: 1 2 3 4 5 6 7 8 9 10 Board: ^ / 3; •3 ( ^/ Cup: I •z. 6 JO h H 1? Verified By: I I I Time Fed/Test Solution Renewed (day): (0)J4^ (DlOOS (2)J135_ (3) l^gO (4) \430 (5) 113^ (6)jaa0_ (7) Notes; d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) 0) Mb (/^K-i^r^lib^iy.-k^ ieTt ^•^t)foH-?oi^)LJ'^i -liir cciM/i^jm.f. ~ QC Check:J>F')lfglu)0'^ Final Review: ,£ ^^T^t Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample iD: Dudek & AssociatesA/Vell 1 Test Number: 0%*^ - 50 ^-D Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 4/23/2009 ) <S> End Date/Time: 4/30/2009 [60S Conc. Rep , Rand Daily Reproduction/ Survival QC # 1 2 i^ mm& 5 6 7 iiii^iiS;:^; - Total QC 2.^7o 1 31 0 o 2-r£> <\ is' au. 2.^7o 2 57 0 0 tC5 ,aa. 3 20 f) 0 t> 0 o 4 13 (5 o o 4 O 4 "0 5 41 © o o 7 0 u 6 45 0 o u 3 (9 0 7 9 0 o 5 0 8 8 35 0 c -o 4 0 \\ —^ ^— 9 30 0 o u s 0 9 t m 10 . 55 0 0 4 O (o a Tech: H? W MD - Conc. Rep Rand Daily Reproduction/ Survival Total ; QC Rep # m-Tm 2 ':i.i;i3;i.i:^ 4 mms iiii ;i6::;.:: iiii^iii^iii^i 8 Total ; QC 1 , 4 0 0 ^•^^ 0 3 la 2 37 0 0 o o 8 —> 27 3 25 0 c? s 0 & \ la.-4 46 5 o 0 o •2: 11 5 42 n o 1 (5 lu 6 65 0 0 r:) 0 4 V7 ' au 7 61 0 o o 0 \3 PM i 8 43 0 0 4 0 & v4 9 5 0 u 0 . 5 8 8 — ' -i41ci.-10 14 0 z> ^> 3 0 8 1 Conc. Rep Rand Daily Reproduction/ Survival Total : QC Rep # 1 ^m^-^m. 3 4 i;i:i:5;;i;H; 'iiiiiiSiiiiii iii:ii|i^i:^:. 8 Total : QC 1 47 O o o o 0 7 : 2 67 e o D o 5 o 3 27 0 6 . O 0 7 o Q : 4 4 22 0. t> 0 0 S i'7 J • IZ il 5 59 <3 o Q » * — • 15' JL! 6 60 cO o 4 O 6 17 7 36 0 !c> 0 V M ?> 1 8 63 0 <i o O 5 1U> 9 29 0 0 D \ S o Le 10 15 -5 o V O 5 o S Neonates f or each replicate were blocked across concentrations at tG.<;t i nitiation 1. Rep: 1 2 3 4 5 6 7 8 9 10 Board: ^( ^( 9./ ^( 5.1 31 "S- 1 Cup; i 4 1 o il 1 2-Verified By: pr (O)J^o (1 )iooc; {2)jQ£ (3) ]7Sv (4) i4.=!)Q (5)ll3S (6)jaOO (7) Notes: d = dead; M = male; UP = lost in progress; B • 4th brood (only the firsts broods are included in total) QC Check: DF ^|U10^ Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Final Review: C-lSl-^^ Freshwater Chronic Bioassay Client: Dudek Sample ID: Well t Water Quality Measurements Test Species: C. dubia Start Date/Time: 4/23/2009 Test No: O^OM-SblO End Date/Time: 4/30/2009 \ 6 OS* Concentration Lab Control ' Day 0 1 3 1 4 1 5 1 6 1 7 ., ' Initial PH 7.4\ <^.o3 DO (mq/L) 7-4 7 5 7.'6 7-4 Cond. (pmhos/cm) :2o4 142 7Q6 -211 M7 Temp CO 15 7 •^4^ 62 7S.^ ^ ' , Final ^ - - . pH 7,'?3 DO (mq/L) 'ir^(l ^.7' - — * "8. a Temp (°C) , ^^47 a/1,7 z^-ia Concentration Conductivitv Controf Day 0 i 1 2 3 1 4 1 5 6 7 " , . • . (nifi'al PH 7.^4 7.«3 8-15 s:. c3 DO (mq/L) 7.?? 7-^ ^•5 77^2 7-5 Cond. (pmhos/cm) :-iD76 -SISD 3300 iiigiSi^-i fempilC) .25.0 2_5-& <s?4.4 iiigiSi^-i oH y.lW ' -7<l( 7.41 DO (mq/L) 7.9 Temp (°C) aHrS-ZM 3 .3M . <r Concentration 6.25% Day . 0 3 4 5 6 7 Initial • PH 7:7 a," 7.S4^ 1 '\'\ ^ii'gS^i^ii^ii DO (mq/L) 7."7 «.4 -7.% •7.4 7.5 ^ii'gS^i^ii^ii Cond. (pmhos/cm) 4"57 HIT Temp (°C) ^SX i3 5.(^ ,S.7,> 7-M.t3 ^4.7 , . Final ijj^^ijia:::;;.^:; «.a;6^ g'.fO DO (mg/L) f 4 ft Temp CC) i?4.i •-L/.io 2.M-3 n Concentration 12.5% Day 0 1 .2 i 3 1 4 1 5 1 6 1 7 ... ~ Initial PH /.GJ-I 7.S<=:> i 7-S'7 7.-/2 ~i-0>'2-7 . ^2. DO (mq/L) 7-^ 1.1 7-5 72 Cond. (pmhos/cm) 57^ Lfoq WMi^S: •^;'^yS^;<r'.--: iiSpii Tamp (°C) ^ '^.^ '25. 2CvO f l-'-A, 7 - WMi^S: •^;'^yS^;<r'.--: iiSpii rin-il pH ^^4'& 1 •0 (mq/L) •if.7 1,6 P E L Temp CO ^M, LP ( 0 1 2 3 4 5 6 7 Analysts: Inifial; ^ ! OP MP Final; SL SL Sample Used (A, B, C): lAr-A IV Comments; Animal Source/Date Received: XK'^AO^K-I <^ I 1 /V^ Sample Log-in Numbers: A: ()^ ~ CT^, B; QC Check: DF 5|ljs|o'^ Nautilus Environmental 5550 Morehouse Drive, Suite ISO. San Diego, CA 92121. Animal Age at Initiation: <dl^(-ir'> C: ""^ FinnI Review: Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek Test Species: C. dubia Sample ID: Well 1 Test No: Start Date/Time: 4/23/2009 I CROM -SQ40 End Date/Time: 4120/2009 I 6 ^ S' Concentration 25% Oay 0jl|2|3|4|5|6l7 Initial , . pH 7 -2^5 l-lZ l-2\ 750 7.52 7-fto 7-'oa DO (mq(L) l'r4 1-1 76 •S.O 77 7.tf> Cond. ((jmhos/cm) Temp(°C) '2B.'=b c35{^ <LH.7 as-? ' final pH ?:.-27 DO (mq/L) 7.9 6-5 Temp (°C) IW-Cc? a4vS- Concentration 50% Oay o| l| 2(3] 4| 5|6 |7 to tlal 7 IlO pH DO (mq/L) 7 7 7 J> ,7- n , "70Z /-IC 7'<p Cond. ((jmhos/cm) 1744 I7M2 1^,3^1 \itz. Temp CO R^r-7 25^.7-Z4.fl =1 . «nal pH 7") 7 DO (mq/L) ?! Temp {°C) 2H>sr XH-3 Concentration . 100% Day 0 1 1 1 2 1 3 1 4 i 5 1 G 1 7 Initial pH ^.'Hc t77 DO (mq/L) 7.Z> 7^ ^.^ Cond. (pmhos/cm) ^)Oo2-,3130 li6(,0 c Temp CC) ^.•^ a<5.o as:(ri 20. .0 Biiial pH 7.W 7.<?6 7>4^ 7,«7 ft H T--7, "^^ • 0 (mq/L) 7.9 Temp CO) 7^c6 '3iK.S cJHcS- Analysts: Initial: HP E5 1^ MO Final: (/)^ SL Sample Used (A. B, C): A-A A .4r A 1 A Ar A Comments; Animal Source/Date Received: \'r\ifiir)r^O-'\ Sample Log-in Numbers: A: 0^'~O33^'^ B; QC CheclOp5MI^ °1 Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Animal Age at Initiation; <^^€)l-\t^^ C: Final Review: I I Appendix B Sample Receipt Infonnation Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Client; tests Performed: CPV4 Sample ID: hW.W 1 l.p<^jYT.flifr), rWiiSC^'^^PPr^rrs . Test ID No(s).: 0?tfV-S^?O 7^ To°l'!> Sample^B, C) Log-in No. (09-xxxx) Sample Collection Date & Time Sample Receipt Date & Tim Check-in Temperature (°C) Temperature OK? DO (mg/L) pH (units) Conductivity (pS/cm) Salinity (ppt) Alkalinity {mglty Hardness(m9/L)* Total Chlorine (mgfl.) Technician Initials 1 ' fn -tO~\ M 1 7 0. '^75^ V5 16c • = mg/L as CaCOj,' - Measured for freshwater samples only, NA = Not Applicabte Freshwater tests: Control/Dilution Water Source: Additional Control? Marine Tests: Control/Dilution Water Source: LABSW ARTSW Additional Control? Y N =_ Other /Ukalinity:_^_2_- _ Alkalinity: Other: Alkalinity: Alkalinity: Hardness; Hardness: Salinity: _ Salinity:. .^0 Sample Salted w/ artificial salt? Y N Sample salted v^f/brine? Y N If yes, what ppt?. If yes. what ppr?. Sample Checi<-ln Information Sample Descnption: COC Complete? ^ N I) A B_ Rltration? .C_ Y Pore Size: Organisms or Aeration? Y Debris Length of Time: Final DO: Final pH: pH Adjustment? Initial pH: Final pH: CI2 Adjustment? t) Initial Free Clj: STS added: Final Free Cij: Sub.«amples for additional chemistry: Comnnents: Temperature for sample must be 0-6'C if received >24 hours past collection time. QC Check: ^l^O-} Final Review: Appendix C Chain-of-Custody Form • CAUFORNIA 5550 MoieliaBaOiive'Site 150 Son Diego, Colitoin 92121 te 858.587.7333 Fox 858.587,3961 • WASHINGTON 5009PodicHiglMt)yEost-StitE2 Toc(siiQ,Wosliiigton 98424 te 253.922,4296 fox 253,922,5814 • BRITISH COLUMBIA 8664 Commerce Court Buinoby British Columbio CaiiodoV5A4N7 te 604,420.8773 Fax604.357.1361 Dote Chain of Custody V^2/0i P„,e of <:„.pi.r.ii.«„„.,. n^SCH. rl^AgQUAK:^ ANALYSES REQUIRED Invoice to: Company :.'..-.gi..;. •. Address 6«>S' ^DyiK^P s-( Address State Cily Stote Zio Contact > UJ Phone/Email , (iJ'T^. Phone/Emai :> >-* S. :• -1U>-W SAMPLE ID DATE Tif/E /AWRIX CONTAINER TYPE NUMSER OF CONTAINERS COMMENTS at 1 Tr3<^ y ,7w PROJECT INFORMATION CUENT P.O, NO, SHIPPED VIA: SAMPLE RECEIPT TOJJM NO OF CONTAINERS , •REC'D GOOD CONDITTON REUNQUISHED BY |CUENT) T SPECIAL INSTRWtnOMS/COMJ^ENTS; REUNQUISHED BY (COURIER) (Time) (Signoture) (PrintBd Name) (Compo ny)' (Date) (Printed Nome) (Company) RECEIVED BY (COURIER) RECEiyEDtBY (LABOR (Signature) (Time) (Printed Name) (Date) (PiJri®i,Nafe6f;i-,,.,.,,,, (Compony) Nc:u^lus£nvl•onmcrlalLog n No (Time) (Dote) -ilKi'!?): Additional costs may be required for sample disposol or storage. Nel 30 unless otiierwise contrc:ct«d. DISTRIBLmON: WHITE - Nauhlus Environnnental. COLOR • Originator Appendix D Qualifier Codes for Datasheets Glossary of Qualifier Codes: Q1 - pH out of recommended range Q2 - Temps out of recommended range, refer to CAR Q3 - DO out of recommended range, refer to CAR Q4 - Salinity out of recommended range, refer to CAR Q5 - Miscalculation Q6 - Spilled test chamber/ Lost test animal Q7- Instrumentation Error/Failure Q8 - Inadequate sample volume, 50% renewal Q9 - Inadequate sample volume, no renewal performed Q10 - Sample out of holding time Q11 - Refer to QA section of report for explanation Q12 - Supplemental information is footnoted Q13 - Test initiated with an incorfect number of test organisms Q14 - Replicate(s) not initiated Q15 - Survival counts not recorded due to poor visibility or heavy debris Q16 - Test placed on air due to DO levels dropping below 4.0 -mg/L 017 - Test initiated on air due to an anticipated drop in DO 018 - Airline obstructed or fell out of replicate and replaced, drop in DO may occur 019 - Animals initiated out of appropriate age range Q20 - Readings not taken, tech error 021 - Replicate not counted, tech error 022 - DO outside of 6-9mg/L but remained within the 100% saturation requirement, % saturation recorded in comments section of datasheet. Updated 11/12/08