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Home My WebLink About; Crossings at Carlsbad Annual Water Quality 2009; Crossings at Carlsbad Annual Water Quality 2009; 2009-08-01I I I I I I I I I I I I I I I I Water Quality Monitoring Annual Report for the Crossings at Carlsbad Carlsbad, California AUGUST 2009 City of Carlsbad Recreation Department 1200 Carlsbad Village Drive Carlsbad, California 92008 PREPARED BY: Dudek 605 Third Street Encinitas, California 92024 Water Quality Monitoring Annual Report for the Crossings at Carlsbad Carlsbad, California Prepared for: City Of Carlsbad Recreation Department 1200 Carlsbad Village Drive Carlsbad, California 92008 Contact: John Cahill Prepared by: DUDEK 605 Third Street Encinitas, California 92024 Contact Doug Gett/nger AUGUST 2009 Water Quality Monitoring Annual Report for the Crossings at Carlsbad TABLE OF CONTENTS Section Page No. 1.0 INTRODUCTION 1 2.0 DISCUSSION OF MONITORING RESULTS 7 2.1 Nitrate 7 2.2 Nitrite 7 2.3 Phosphorus 7 2.4 Total Suspended Solids 8 2.5 Dissolved Oxygen 8 2.6 Hydrogen Ion Activity 8 2.7 Salinity 8 2.8 Toxicity 9 3.0 RECOMMENDATIONS 9 4.0 CONCLUSION 9 5.0 REFERENCES 9 APPENDIX A Laboratory Test Results LIST OF TABLES 1 Project Timeline Showing Construction Milestones and Water Quality Monitoring 1 2 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results Upstream Surface Location 3 3 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results Upstream Well Location 4 4 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results Downstream Surface Location 5 5 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results Downstream Well Location 6 5938-00 D U D E K i August 2009 Water Quality Monitoring Annual Report for the Crossings at Carlsbad INTENTIONALLY LEFT BLANK 5938-00 D U D E K ii August 2009 Water Quality Monitoring Annual Report for the Crossings at Carlsbad 1.0 INTRODUCTION This annual report documents results of water quality monitoring for calendar year 2008 undertaken at the Crossings at Carlsbad golf course in Carlsbad, California. Water quality monitoring is required by Coastal Development Permit No. A-6-CII-00-087, Special Condition No. 7. P & D Environmental prepared the City of Carlsbad Municipal Golf Course Water Quality Monitoring Plan (Plan), dated November 18, 2004. The Plan outlines where sampling is to occur, sampling frequency, and testing requirements. The Plan also outlines water quality thresholds and outlines when corrective actions are required. Dudek implemented the water quality monitoring program in substantial conformance with the Plan. The Water Quality Monitoring Annual Report for the Crossings at Carlsbad, Carlsbad, California, dated September 5, 2007, (Dudek) describes the installation and initial water quality monitoring that was undertaken during construction and up to the golf course opening in 2007. Water was sampled at four locations as specified in the Plan, consisting of upstream and downstream surface and groundwater well locations. Surface water samples were collected from the creek adjacent to the well locations. In 2008, quarterly samples were collected on February 11, June 11, and September 25. A sampling scheduled for late December 2008 had to be postponed to January 2009 due to laboratory scheduling issues for toxicity testing. The January 2009 test results are included in this annual report. Table 1 graphically shows a timeline when various project related milestones and the water quality monitoring occurred since golf course construction began. Water samples were submitted to EnviroMatrix Analytical, Inc. for chemical analysis and to Nautilus Environmental for toxicity testing. Two labs were used for the testing because the types of analysis for each type of testing are distinctly different and are require different equipment and expertise. Test results were forwarded to the Golf Course Manager after they were received from the labs. Monitoring results are summarized in Tables 2-5, and discussed below. Full laboratory test results are included in Appendix A. Table 1 Project Timeline Showing Construction Milestones and Water Quality Monitoring Year 2005 2006 2007 2008 2009 J M F M M M 3 A M 1 J M M M J M A M .-r. .*>."•• S s ,.'T- . IT 0 c N M D S = Start of Clearing and Grading C = Completion of Vegetation Clearing G = Completion of Mass Grading I = Installation of Monitoring Wells M = Water Quality Monitoring T = Installation of Turf in Macario Canyon Area 0 = Golf Course Open DUDEK 5938-00 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad INTENTIONALLY LEFT BLANK 5938-00 D U D E K 2 August 2009 Water Quality Monitoring Annual Report for the Crossings at Carlsbad Table 2 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results* Upstream Surface Location Date 6-1-06 7-19-06 8-23-06 11-28-06 2-26-07 6-13-07 2-11-08 6-11-08 9-25-08 1-15-09 Nitrate 0.39 0.39 0.28 1.3 0.36 0.89 0.73 0.32 0.45 — Nitrite NO ND ND ND ND ND ND ND ND — DO 3.34 5.49 3.92 4.37 9.15 7.06 5.64 6.02 3.13 7.97 pH 7.24 7.33 7.26 7.50 7.33 7.81 7.75 7.86 7.65 7.66 Phosphorus 0.06 0.05 0.14 ND 0.07 0.30 0.13 0.11 0.06 0.06 TSS ND ND ND ND ND ND ND ND ND ND pH 7.49 7.62 7.78 7.35 7.60 7.87 7.68 7.88 7.72 7.79 DO 8.4 8.7 8.8 10.2 10.8 8.6 10.3 10.1 10.0 10.6 Conductivity 3,910 3,380 3,840 1,890 3,230 3,720 3,860 3,689 3,340 3,510 Hardness >1,000 1,089 >1,000 525 966 >1,000 1,182 1,172 1,086 >1,000 Alkalinity 202 217 182 125 215 167 220 259 178 197 'Results highlighted in grey are baseline measurements done prior to turf installation in the Macario Canyon portion of the golf course. DUDEK 5938-00 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad Table 3 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results* Upstream Well Location Date 6-1-06 7-19-06 8-23-06 11-28-06 2-26-07 6-13-07 2-11-08 6-11-08 9-25-08 1-15-09 Nitrate ND 0.06 ND ND ND 0.05 0.06 0.07 ND — Nitrite ND ND ND ND ND ND ND ND ND — DO 1.10 4.13 0.56 0.11 4.48 2.50 1.68 2.46 1.03 2.81 pH 5.9 6.84 6.03 6.00 5.60 6.06 6.43 5.94 5.85 5.90 Phosphorus 0.17 0.16 0.70 0.27 0.15 1.01 0.48 0.21 0.42 0.23 TSS ND 145 464 175 108 55 465 99 235 27 pH 6.32 6.80 6.58 6.47 6.17 6.64 7.30 6.20 6.34 7.26 DO 4.2 5.6 4.6 7.3 5.8 5.5 5.7 5.4 7.8 5.7 Conductivity 3,720 2,580 3,380 3,450 3,450 3,020 2,460 2,969 3,020 2,670 Hardness 968 585 887 953 936 782 596 886 860 >1,000 Alkalinity 71 110 68 70 57 66 81 90 59 41 'Results highlighted in grey are baseline measurements done prior to turf installation in the Macario Canyon portion of the golf course. DUDEK 5938-00 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad Table 4 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results* Downstream Surface Location Date 6-1-06 7-19-06 8-23-06 11-28-06 2-26-07 6-13-07 2-11-08 6-11-08 9-25-08 1-15-09 Nitrate ND 0.06 ND 0.74 0.07 0.48 0.27 0.23 0.43 — Nitrite ND ND ND ND ND ND ND ND ND — 00 2.92 6.32 4.31 3.97 8.5 7.01 5.06 6.04 3.47 7.60 PH 7.42 7.49 7.40 7.73 7.50 7.82 7.78 7.81 7.84 7.51 Phosphorus 0.21 0.17 0.29 0.08 0.08 0.32 0.17 0.13 0.12 0.08 TSS ND ND ND 31 ND ND 20 ND 29 ND pH 7.79 7.86 7.92 7.32 7.90 7.92 7.87 7.88 7.82 7.80 DO 8.1 8.5 8.9 10.6 10.8 8.3 10.0 9.1 10.6 9.8 Conductivity 3,590 3,430 3,690 2,160 2,490 3,490 3,370 3,559 3,210 3,100 Hardness >1,000 1,000 >1,000 793 684 >1,000 996 1,099 996 >1,000 Alkalinity 245 268 254 64 208 257 152 285 234 255 'Results highlighted in grey are baseline measurements done prior to turf installation in the Macario Canyon portion of the golf course. DUDEK 5938-00 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad Table 5 Carlsbad Municipal Golf Course Water Quality Monitoring Test Results* Downstream Well Location Date 6-1-06 7-19-06 8-23-06 11-28-06 2-26-07 6-13-07 2-11-08 6-11-08 9-25-08 1-15-09 Nitrate 9.28 16.4 20.6 20.2 21.2 19.4 16.1 19.7 31.9 — Nitrite 0.05 ND ND ND 0.14 ND ND ND ND — 00 0.94 2.85 3.93 1.22 5.18 2.81 2.18 3.38 1.35 2.80 PH 6.60 6.56 6.37 6.61 6.27 6.69 6.68 6.65 6.70 6.63 Phosphorus 0.36 0.28 1.68 0.20 0.22 0.31 0.26 0.39 0.26 0.78 TSS 37 100 66 183 179 341 232 299 112 807 pH 6.63 6.79 6.79 6.64 6.73 6.83 6.88 6.80 6.91 7.34 00 2.7 4.8 5.4 8.3 5.2 4.7 7.5 5.7 7.3 5.4 Conductivity 4,820 4,730 4,990 4,980 5,050 4,920 4,850 4,930 4,450 4,320 Hardness >1,000 1,424 >1,000 >1,000 1,574 >1,000 1,465 1,694 1,525 >1,000 Alkalinity 250 278 231 263 236 228 250 262 230 127 'Results highlighted in grey are baseline measurements done prior to turf installation in the Macario Canyon portion of the golf course. DUDEK 5938-00 August 2009 Water Quality Monitoring Annual Report for the Crossings at Carlsbad 2.0 DISCUSSION OF MONITORING RESULTS Each of the constituents monitored is discussed below, followed by recommendations for the future and conclusions. 2.1 Nitrate All of the water samples collected in 2008, with the exception of the downstream well location, have either very low levels or levels below detection limits for nitrate. The lower end detection rate for nitrate is 0.05 mg/1. Surface water samples flowing onto the site at the upstream collection point had nitrate levels ranging from 0.32 to 0.73 mg/1. Surface water samples flowing off the site at the downstream collection point had lower nitrate levels, ranging from 0.23 to 0.43 mg/1. For all the surface water samples, the water leaving the site had lower nitrate levels than the water flowing onto the site from upstream sources. Reductions range from 4% to 63%. There was considerable variation between the upstream and downstream groundwater samples. One of the three upstream groundwater samples had a nitrate level that was undetectable, while the two other samples yielded 0.06 mg/1 and 0.07 mg/1, slightly above the 0.05 mg/1 lower level detection limit. The downstream groundwater samples had moderate to high levels of nitrate. The samples had nitrate levels ranging from 16.1 to 31.9 mg/1. The 31.9 mg/1 nitrate level recorded at the September 25 sampling is out of the normal range for this location. Nitrate levels at the downstream well location have ranged from 9.28 to 21.2 mg/1. All downstream samples had significantly higher nitrate levels than the upstream monitoring location, which has been consistent since the wells were installed. Note that nitrate levels were not measured for the January 2009 sampling due to a paperwork error on the chain-of-custody form. 2.2 Nitrite All of the water samples collected in 2008, both groundwater and surface samples, have levels below detection limits for nitrite. Note that nitrite levels were not measured for the January 2009 sampling due to a paperwork error on the chain-of-custody form. 2.3 Phosphorus With regards to phosphorus levels, test results vary considerably from 0.06 to 0.48 mg/1. Downstream surface water samples had higher phosphorus levels than the upstream surface samples, consistent with previous results. Upstream surface phosphorous levels ranged from 0.06 to 0.13 mg/1 and downstream surface phosphorous levels ranged from 0.08 to 0.17 mg/1. For groundwater samples, upstream phosphorus levels varied from 0.21 to 0.48 mg/1 and downstream phosphorous levels ranged from 0.26 to 0.39 mg/1. 5938-00 D U D E K 7 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad 2.4 Total Suspended Solids Total suspended solids (TSS) measurements for the surface water samples were detected in the downstream sample collected in February and September 2008. Those samples had a TSS reading of 20 mg/1 and 29 mg/1, respectively. TSS measurements were also required for groundwater. TSS levels ranged from 27 to 465 mg/1 in the upstream groundwater samples, and 112 to 807 mg/1 in the downstream groundwater samples. The groundwater TSS levels largely reflect the stirring up of sediment within the well casing when the water is purged prior to collecting the sample. 2.5 Dissolved Oxygen Dissolved oxygen levels have varied significantly for all samples collected, pre-construction and post-construction, surface, and groundwater. Groundwater samples generally have lower dissolved oxygen levels than surface samples, as would be expected. Dissolved oxygen levels will vary depending on water temperature and other factors and can be expected to change throughout the year because of this. Dissolved oxygen levels measured vary between the two testing laboratories. This variance in measurements for the same samples likely results from temperature variations in the lab samples when tested, time variations between when the water samples were collected and analyzed, and perhaps differences in testing methodologies. The testing protocol for dissolved oxygen require the reading to be taken within 15 minutes of sample collection and it is not possible to collect the samples, transport them to any testing lab, and make the measurement within that time frame. 2.6 Hydrogen Ion Activity Hydrogen ion activity, expressed as pH, also vary between the two testing laboratories. Measured pH levels in surface and groundwater samples collected at the site have ranged from 5.85 to 7.88 in 2008. The baseline groundwater samples had measured pH levels ranging from 5.9 to 7.92, several of which fall outside the specified "acceptable" range of 6.5 to 9.0, as stated in the Plan. The natural, baseline condition of the groundwater in this area appears to be acidic and salty. This can probably be attributed to the marine geology in the area (Bowman 1973). 2.7 Salinity Conductivity measurements have shown that the water collected from both groundwater and surface locations have a high salt content. Surface water conductivity measurements ranged from 3,100 to 3,860 mg/1 for the surface water samples. The groundwater samples measurements varied between upstream and downstream well locations. Upstream well locations measurements ranged from 2,460 to 3,020 mg/1, and 4,320 to 4,930 mg/1 at the downstream sampling location. 5938-00 D U D E K 8 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad This can probably be attributed, at least in part, to the marine geology in the area (Bowman 1973). 2.8 Toxicity Toxicity testing was conducted for all water samples collected. Conductivity measurements showed that nearly all samples tested had high enough salt levels to cause reduced reproduction for the water fleas (Ceriodaphnia dubia) used for toxicity testing. Because of this, the laboratory conducted additional conductivity control tests to determine if something other than the high salt levels was causing the observed water flea mortality. Test results indicate that elevated conductivity levels in the samples can be attributed to the observed adverse effect in water flea reproduction. 3.0 RECOMMENDATIONS As was stated in last year's report (Dudek 2007), Dudek believes there are significant problems with the water quality standards listed in Section 9.0 of the Plan, "Interpretation of Results." The Plan is inadequate in that it does not specify sampling protocols or procedures, and does not establish a scientific basis for setting threshold values for action levels that vary from the measured baseline levels. The standards have limited relevance to the stated water quality objectives and should be modified to provide useful guidelines and action levels. Dudek's hydrogeology group is preparing revised water quality monitoring guidelines and standards for the City to present to the Coastal Commission. 4.0 CONCLUSION Water quality monitoring results during the 2008 period show no significant adverse water quality issues resulting from golf course operations, though some measured levels do fall within the established corrective action guidelines in the approved Plan. There is no data to show any adverse trends developing from golf course operations with regards to water quality for the area. 5.0 REFERENCES Bowman, R.H. 1973. Soil Survey, San Diego Area, California, Part 1. United States Department of the Agriculture. 104 pp. + appendices. Dudek. 2007. Water Quality Monitoring Annual Report for the Crossings at Carlsbad, Carlsbad, California. September 5. 11 pp + appendix. P & D Environmental. 2004. City of Carlsbad Municipal Golf Course Water Quality Monitoring Plan. November 18. 9 pp. 5938-00 DUDEK 9 August 2009 Water Quality Monitoring Annual Report for the Crossings At Carlsbad INTENTIONALLY LEFT BLANK 5938-00 D U D E K 10 August 2009 APPENDIX A Laboratory Test Results February 22, 2005 EnviroMatrix [Ml Analytical, Inc. 22 February 2008 Dudek & Associates, Inc. EMA Log #: 0802198 Attn: Doug Gettinger 605 Third Street Encinitas, CA 92024 Project Name: 5938-01 Carlsbad Golf Course Enclosed are the results of analyses for samples received by the laboratory on 02/11/08 20:24. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. I certify that this datais in compliance both technically and for completeness. Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewridge Avenue, Suite A • San Diego, California 92123 • (858) 560-7717 • Fax (858) 560-7763 Analytical Chemistry Laboratory Client Name: Dudek & Associates, Inc. EMALog#: 0802198 Project Name: 5938-01 Carlsbad Golf Course ANALYTICAL REPORT FOR SAMPLES [ Sample ID Laboratory ID Matrix Date Sampled Date Received Welll 0802198-01 Grad-Water 02/11/0813:30 02/11/0820:24 Surface 1 0802198-02 L«H»«i 02/11/0813:45 02/11/0820:24 Well 3 0802198-03 Grnd-Water 02/11/0812:15 02/11/0820:24 Surfaces 0802198-04 Liquid 02/11/0812:15 02/11/0820:24 'Tie results in this report apply to the samples analyzed hi accordance with the chain of •ustody document. This analytical report must be reproduced in its entirely. EnviroMatri* Rtf 1 Analytical. Inc. Page 2 of 8 Client Name: Dudek & Associates, Inc. Project Name: 5938-01 Carlsbad Golf Course EMALog#:0802198 Conventional Chemistry Parameters by Standard/EPA Methods Anaryte Result Well 1 (0802198-01) Grnd-Water Sampled: 02/11/08 Nitrate as N Nitrite as N Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids Surface 1 (0802198-02) Liquid Nitrate as N Nitrite as N Dissolved Oxygen pH Phosphorus, Total Total Suspended Solids 0.06 ND 1.68 6.43 0.48 465 Sampled: 02/11/08 13 0.73 ND 5.64 7.75 0.13 ND ReportLimi 13:30 0.05 0.05 0.10 0.10 0.05 20 ing t Units Dilution Received: 02/11/08 20:24 mg/t 1 n 11 „ pH Units mg/1 " n n Batch 8021928 8021224 8022038 8021301 8022102 8021424 Prepared 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/15/08 Analyzed 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/16/08 Method SM4500N03E SM4500NO2B SM4500-O G SM4500-H+ B SM4500PB.E SM2540 D Notes W-02 HT-15 HT-15 :45 Received: 02/11/08 20:24 0.05 0.05 0.10 0.10 0.05 20 Well 3 (0802198-03) Grnd-Water Sampled: 02/11/08 12:15 Nitrate as N Nitrite as N Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids Surface 3 (0802198-04) Liquid Nitrate as N Nitrite as N Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids 16.1 ND 2.18 6.68 0.26 232 Sampled: 02/11/08 12 0.27 ND 5.06 7.78 0.17 20 1.25 0.05 0.10 0.10 0.05 20 mg/1 1 M 11 " pH Units mg/1 M H Received: 02/11/08 20:24 mg/1 25 1 n n pH Units " mg/1 " ti rf 8021928 8021224 8022038 8021301 8022102 8021424 8021928 8021224 8022038 8021301 8022102 8021424 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/15/08 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/15/08 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/16/08 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/16/08 SM4500 N03 E SM4500 NO2 B SM4500-OG SM4500-H+ B SM4500 P B, E SM2540 D SM4SOONO3E SM4500NO2B SM4500-OG SM4SOO-H+ B SM4500 P B, E SM2540D W-02 HT-15 HT-15 W-02 HT-15 HT-15 :15 Received: 02/11/08 20:24 0.05 0.05 0.10 0.10 0.05 20 mg/1 1 r« II it ti pH Units " mg/1 " n n 8021928 8021224 8022038 8021301 8022102 8021424 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/15/08 02/20/08 02/12/08 02/12/08 02/12/08 02/21/08 02/16/08 SM4500NO3E SM4500NO2B SM4500-O G SM4500-H+ B SM4500 P B, E SM2540D W-02 HT-15 HT-15 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report mast be reproduced In its entirety. • EmriroHatrix Analytical, Inc.Page 3 of 8 Client Name: Dudek & Associates, Inc. Project Name: 5938-01 Carlsbad Golf Course Conventional Chemistry Analyte Result EMALog#: 0802198 Parameters by Standard/EPA Methods - Quality Control Reporting Spike Source %REC RPD Limit Units Level Result %REC Limits RPD Limit Notes Batch 8021224 Blank (8021224-BLK1) Nitrite as N LCS(8021224-BS1) Nitrite as N LCS Dup (8021224-BSD1) Nitrite as N Duplicate (8021224-DUP1) Nitrite as N Matrix Spike (8021224-MS1) Nitrite as N Matrix Spike Dup (8021224-MSD1) Nitrite as N Batch 8021301 Duplicate (8021301-DUP1) pH Reference (8021301-SRM1) PH Batch 8021424 Blank (8021424-BLK1) ND 0.12 0.12 0.01 0.11 0.11 6.41 9.19 Prepared & Analyzed: 02/12/08 0.05 mg/1 Prepared & Analyzed: 02/12/08 0.05 mg/1 0.100 120 80-120 Prepared & Analyzed: 02/12/08 0.05 mg/I 0.100 120 80-120 0 20 Source: 0802198-04 Prepared & Analyzed: 02/12/08 0.05 mg/1 0.01 0 20 Source: 0802198-04 Prepared & Analyzed: 02/12/08 0.05 mg/1 0.100 0.01 100 80-120 Source: 0802198-04 Prepared & Analyzed 02/12/08 0.05 mg/1 0.100 0.01 100 80-120 0 20 Source: 0802198-01 Prepared & Analyzed: 02/12/08 0.10 pH Units 6.43 0.3 20 HT-15 Prepared & Analyzed: 02/12/08 0.10 pH Units 9.10 101 97.8-102.2 Prepared & Analyzed: 02/14/08 Total Suspended Solids ND 20 mg/1 The results in this report apply to the samples analyzed in accordance with the chain of custody document This analytical report must be reproduced in its entirety. - BnviroMatrix Analytical, In Page 4 of 8 Client Name: Dudek & Associates, Inc. Project Name: 5938-01 Carlsbad Golf Course Conventional Chemistry Analyte Result EMALog#: 0802198 Parameters by Standard/EPA Methods - Quality Control Reporting Spike Source %REC RPD Limit Units Level Result %REC Limits RPD Limit Notes Batch 8021424 Duplicate (8021424-DUP1) Total Suspended Solids 186 Reference (8021424-SRM1) Total Suspended Solids 70 Batch 8021928 Blank (8021928-BLK1) Nitrate as N ND LCS (8021928-BS1) Nitrate as N 0.44 LCS Dup (8021928-BSD1) Nitrate as N 0.50 Duplicate (8021928-DUP1) Nitrate as N 0.97 Matrix Spike (8021928-MS1) KitrateasN 3.06 Matrix Spike Dup (8021928-MSD1) Nitrate as N 3.16 Reference (8021928-SRM1) Nitrate as N 0.56 Source: 0802231-01 Prepared & Analyzed: 02/14/08 20 rag/1 208 11 20 Prepared & Analyzed: 02/14/08 20 rag/I 74.5 94 85.9-104.3 Prepared & Analyzed: 02/19/08 0.05 rog/1 Prepared & Analyzed: 02/19/08 0.05 mg/1 0.500 88 80-120 Prepared & Analyzed: 02/19/08 0.05 mg/1 0.500 100 80-120 13 20 Source: 0802086-05RE1 Prepared & Analyzed: 02/19/08 0.05 rog/1 0.94 3 20 Source: 0802086-OSREI Prepared & Analyzed: 02/19/08 0.25 mg/1 2.50 0.94 85 80-120 Source: 0802086-05RE1 Prepared & Analyzed: 02/19/08 0.25 mg/1 2.50 0.94 89 80-120 3 20 Prepared & Analyzed: 02/19/08 0.05 mg/1 0.621 90 89.2-108.5 The results in this report apply to the samples analyzed in accordance with the chain of xtstody document, this analytical report must be reproduced in its entirety. EnriroHatrix Analytical, Ini Page S of8 Client Name: Dudek & Associates, Inc. Project Name: 5938-01 Carlsbad Golf Course Conventional Chemistry Analytc Result EMALogft 0802198 Parameters by Standard/EPA Methods - Quality Control Reporting Limit Units Spike Source %REC RPD Level Result %REC Limits RPD limit Notes Batch 8022038 Duplicate (8022038-DUP1) Dissolved Oxygen 1.64 Duplicate (8022038-DUP2) Dissolved Oxygen 5.33 Duplicate (8022038-DUP3) Dissolved Oxygen 2.17 Duplicate (8022038-DUP4) Dissolved Oxygen 4.97 Batch 8022102 Blank (8022102-BLK1) Phosphorus, Total ND LCS (8022102-BS1) Phosphorus, Total 0.56 LCS Dup (8022102-BSD1) Phosphorus, Total 0.56 Duplicate (8022102-DUP1) Phosphorus, Total 0.53 Matrix Spike (8022102-MS1) Phosphorus, Total 1.02 Source: 0802198-01 0.10 mg/1 Source: 0802198-02 0.10 mg/1 Source: 0802198-03 0.10 mg/I Source: 0802198-04 0.10 mg/1 0.05 mg/1 0.05 mg/1 0.05 mg/1 Source: 0802198-01 0.05 mg/1 Source: 0802198-01 0.05 mg/1 Prepared & Analyzed 02/12/08 1.68 2 20 Prepared & Analyzed 02/12/08 5.64 6 20 Prepared & Analyzed 02/12/08 2.18 0.5 20 Prepared & Analyzed 02/12/08 5.06 2 20 Prepared & Analyzed 02/21/08 Prepared & Analyzed 02/2 1/08 0.500 112 80-120 Prepared & Analyzed 02/21/08 0.500 112 80-120 0 20 Prepared & Analyzed 02/21/08 0.48 10 20 Prepared & Analyzed 02/2 1 /OS 0.500 0.48 108 80-120 HT-15 HT-15 HT-1S HT-15 The results in this report apply to the samples analyzed in accordance with the chain of vstody document This analytical report must be reproduced In its entirety. • EnviroMatrix I /Mf, I Analytical, Ii Page 6 of8 Client Name: Dudek & Associates, Inc. EMALogfc 0802198 Project Name: 5938-01 Carlsbad Golf Course Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Reporting Spike Source %REC RPD Analyte Result Limit Units Level Result %REC Limits RPD Limit Notes Batcb 8022102 Matrix Spike Pup (8022102-MSD1) Source; 0802198-01 Prepared & Analyzed: 02/21/08 Phosphorus, Total 1.00 0.05 mg/1 0.500 0.48 104 80-120 2 20 The results in this report apply la Ae samples analyzed in accordance with the chain of custody document This analytical report must be reproduced in its entirety. • BnviroMatrix [ ,M 1 Analytical, Inc. Page 7 of 8 Client Name: Dudck & Associates, Inc. EMALog#: 0802198 Project Name: 5938-01 Carlsbad Golf Course Notes and Definitions W-02 The sample for nitrate analysis was preserved with H2SO4 after the nitrite portion of the analysis was completed to extend the holding time for the sample. Nitrate results are corrected for the nitrite contribution pa- the method. HT-15 This sample was received outside of the EP A's recommended 15 minute holding time for mis analysis. However, the sample was analyzed immediately upon receipt ND Analyte NOT DETECTED at or above the reporting limit NR Not Repotted dry Sample results reported on a dry weight basis RPO Relative Percent Difference lie results in this report apply to the samples analyzed In accordance with the chain of Custody document. This analytical report must be reproduced in its entirety. EnTiroBUtrix [ftlL J Analytical, toe. BageSofS- CHAIN-OF-CUSTODY FORM pagej—°f (Analytical, Inc. MALOG* O £02 4340 Viewrid e Avt, Ste. A • San Diego, CA 9M33 • Phone ( 58) 560-7717 • Fan (858) 560-7763 ^Vft- Sample Date/Time Contalner(s) # of /Type Simple Matrix Codti: GW-ground water WW«wastt water DW'dnnking water Sw=storm water A-air SED-sediment O-oil T-tiwue S-iolid otber (jpeciry)f Illiquid other (specify) ier HUPS nFedEx HUSPS OOient drop off DOthei; Turnaround Time: RSamcday H24hr 048 hi njday TMday r,5 diy A<fD(7day) Reporting Kcqulnmtntt: UFax WDF DExcel OGcotrackcr EDF HOlher Simple DIspoul: n*iji Laboratory ll'Rctum to client: F/U or Dtlivcty n Archive until Conlainers Properly PrMerved: Yes No N/A Temperature@Receipt COOLibete Agree: Yes No NM Sampled By: DClient OJBMA CAutosampler White - EMA Canary - Accounting Fink - Client (w/Repon)Goldeorod - Client (Relinquish Samples)'Additional costs may apply, consult a project manager for details. 'EMA reserves the right to return any samples that do not match our waste proiUe. Note: By relinquishing samples to EnviroMatrix Analytical, Inc., client agrees to pay for the services requested on this chain of custody form and any additional analyses performed on this project. Payment for services is due wilhin 30 days from the date of the invoice. Sample(s) will be disposed of after 30 days unless otherwise noted. All work is subject to EMA's terms and conditions. California 5550 Morehouse Drive Suite 150 .Son Diego, CA 92121 858-587-7333 fax:858-587-3961 Washington 5009 Pacific Highway East Suite 2 TacomaWA 98424 .253-922-1296 fax:253-922-5814 British Columbia *8664 Commerce Court Bumaby, British Columbia V5A4N7 604-603-9381 fax:604-603-9381 Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Testing Period: February 2008 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 April 8, 2008 Data Quality Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified Date: #-#- TOXICITY SUMMARY REPORT Test ID Number 0802-S056 a, b, c, and d Client: Dudek & Associates Monitoring Period: February 2008 Sample Information Client: Dudek & Associates, Inc. Project Study: Carlsbad Golf Course Toxicity Evaluation - February 2008 Sample Site ID's: a) Well #1 b) Well #3 c) Surface 1 d) Surface 3 Sample Collection Date, Time: 2/11/08,12:15 to 13:45 Sample Receipt Date, Time: 2/12/08,14:30 Sample Material: Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Controls: Protocol Used: Test Acceptability Criteria: Test Validity: 2/12/08 to 2/19/08 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, < 8 hours 100, 50, 25,12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity = 200 uS/cm Lab freshwater spiked with seawater to raise the conductivity to levels matching the samples (range: 2,460 to 4,850 uS/cm). These controls were conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic EPA/821/B-00/004,2000 Method Guidance The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Test results met all test acceptability criteria and internal QA/QC requirements. Table 1. Water Quality Information: Samples Upon Receipt Sample ID Well #1 Well #3 Surface 1 Surface 3 PH 7.30 6.88 7.68 7.87 DO (mg/L) 5.7 7.5 10.3 10.0 Temp (°C) 5.4 5.9 6.0 5.5 Conductivity (jtS/cm) 2460 4850 3860 3370 Hardness (mg/L as CaCO3) 596 1465 1182 996 Alkalinity (mg/L as CaCO3) 81 250 220 152 Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates -TesUP Number: 0802-S056 a, b, c, and d Monitoring Peripd: February 2008 Table 2. Summary of Test Results Sample ID Well #1 Well #3 Surface 1 Surface 3 Concentration (%) Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Mean Percent Survival 100 100 100 100 80 100 90 100 100 100 90 80 22* 70 100 100 100 100 100 100 70 90 90 100 100 100 70 70 Mean Number of Offspring Produced 32 26* 21* 14* 8* 6* 15* 27 23 24 14* 8* 0* 1 * 27 27 27 25 22 7* 11 * 23 23 26 25 23 8* 8* * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Note: the Conductivity Control with each test had a conductivity level, which matched that of the 100% sample material. As can be seen from the data above, the matching conductivity levels resulted in a similar adverse effect to the organism's reproduction. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: Q802-S056 a, b,c, and d . . „ __.JJJonj^jflng^ejngd:Fefar!M!!yjOM.. Table 3. Summary of Statistical Results and Reportable Values Sample ID Test & Endpoint Welitt: Survival Reproduction Well #3: Survival Reproduction Surface 1: Survival Reproduction Surface 3: Survival Reproduction NOEC (% effluent) 100 <6.25 50 12.5 100 50 100 50 ECM (% effluent) >100 21.7 68.9 27.5 >100 79.2 >100 86.1 TUc (toxicity units) 1.0 >16 2.0 8.0 1.0 2.0 1.0 2.0 NOEC = the highest Concentration that produces No Observed Effect ECso ~ concentration that causes an adverse effect to 50% of the test organisms Chronic Toxicity Units (TUc) = 100 4 NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. The Percent Minimum Significant Difference (PMSD), which is a measure of variability, was slightly below the acceptable range in the Well 1 test (12% vs. a range of 13 - 47%). This could lead to over-sensitive statistics, and may detect differences as low as 12%. However, the mean difference in reproduction between the control and 6.25% concentration was 18.4%, which is greater than the detection limit and therefore statistically significant (according to the EPA protocol). In three of the samples, there were no adverse effects observed with the survival of the test organisms. However, site Well 3 did produce a statistically significant decrease in survival in the 100% concentration, when compared to the survival in the lab control. There was also an adverse effect or toxicity observed in all four samples with regards to the reproduction endpoint The two Surface samples resulted in toxicity at the 100% concentration only, while Well 3 resulted in toxicity down to the 25% concentration and Well 1 at the 6.25% concentration. The adverse effects to reproduction were expected however, due to the elevated levels of conductivity found in the samples. Through internal studies and experience, we have consistently seen that adverse effects begin to show in the reproduction endpoint when conductivity levels exceed 2,500 uS/cm. Conductivity levels are determined by the varying concentration of salts in a sample. The optimum range for Ceriodaphnia is between approximately 100 and 1,500 uS/cm. The correlation between higher conductivity levels and decreased reproduction in Ceriodaphnia was observed in this study as well. Since all four samples had conductivity levels at or greater than 2,500 uS/cm (ranging from 2,460 to 4,850 uS/cm), an adverse effect to the reproduction was Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test.i^NumMn..OM^ogBg,.^,.g,.and.d . MonjjQring.Perigdj.feJlguajy.2008.. expected. The Well # 3 sample had the highest conductivity level (4,850 uS/cm), and as expected, showed the greatest decrease in the number of offspring produced (zero reproduction in the 100% concentration). Along with each sample tested, a Conductivity Control was tested. The Conductivity Control was standard laboratory water with natural seawater added to raise the conductivity level to match that of the 100% sample. Each Conductivity Control, with its corresponding 100% sample, resulted in similar reductions in the reproduction rate. The results of this study indicate that the elevated conductivity levels found in the samples may have contributed to the adverse effects observed in reproduction of the Ceriadaphnia test species. Nautilus Environmental - San Diego Bioassay Laboratory Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis Site: WelM CETIS Summary Report Report Date: Link/Link Code: 02Apr-0811:43(p1 of 2) 12-9976-2955/0802-S056C Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 16-6401-8616 Test Type: Reproduction-Survival (7d) Start Date: 12 Feb-08 15:15 Protocol: EPA/821/R-02-013 (2002) Ending Date: 19 Feb-08 10:30 Species: Ceriodaphnia dubia Duration: 6d 19h Source: In-House Culture Sample No: 11-7396-8136 Code: 08-0168 Sample Date: 11 Feb-08 13:30 Material: Groundwater Receive Date: 12 Feb-08 14:30 Source: Dudek & Associates Sample Age: 26h(5.4'C) Station: Well 1 Comparison Summary Analysis No Endpoint 05-9844-4642 7d Survival Rate 18-4393-2691 Reproduction Point Estimate Summary Analysis No Endpoint 06-9939-2046 Reproduction Test Acceptability Analysis No Endpoint 05-9844-4642 7d Survival Rate 06-9939-2046 Reproduction 18-4393-2691 Reproduction 18-4393-2691 Reproduction NOEL LOEL 100 > 100 <6.25 6.25 Effect-% 50 Attribute Control Resp Control Resp Control Resp PMSD Conc-% 21.7 Test Stat 1 31.6 31.6 0.123 TOEL N/A N/A Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <8h Client Dudek & Associates Project: PMSD N/A 12.3% Method Fisher Exact/Bonrerroni-Holm Test Dunnetfs Multiple Comparison Test 95% LCL 95% UCL Method 18.8 28.1 Linear Interpolation (ICPIN) Acceptably Limits Overlap 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria .. Fails acceptability criteria ^Ijvj 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 10 Mean 0.9 1 1 1 1 0.8 1 95% LCL 0.782 1 1 1 1 0.643 1 95% UCL 0.957 1 Min 0 1 1 1 1 0 1 Max 1 1 1 1 1 1 1 StdErr 0.0577 0 0 0 0 0.077 0 StdDev 0.316 0 0 0 0 0.422 0 CV% 35.1% 0.0% 0.0% 0.0% 0.0% 52.7% 0.0% Diff% 0.0% -11.1% -11.1% -11.1% -11.1% 11.1% -11.1% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 10 Mean 15 31.6 25.8 21 13.9 7.7 6.3 95% LCL 12.1 30.7 24.5 19.7 12.3 6.02 4.74 95% UCL 17.9 32.5 27.1 22.3 15.5 9.38 7.86 Min 1 27 21 17 7 0 0 Max 28 35 31 27 21 14 11 StdErr 1.43 0.457 0.625 0.65 0.781 0.821 0.76 StdDev 7.83 2.5 3.43 3.56 4.28 4.5 4.16 CV% 52.2% 7.92% 13.3% 16.9% 30.8% 58.4% 66.1% Diff% 0.0% -111.0% -72.0% -40.0% 7.33% 48.7% 58.0% PM6P m V_x» 000-089-163-3 CETIS™ v1.6.3revE Analyst:QA:: L * CETIS Summary Report Report Date: Link/Link Code: 02Apr-0811:43(p2of 2) 12-9976-2955/0802-S056C Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6,25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 1 Rep1 7 31 22 17 12 9 3 Rep 2 1 1 1 1 1 0 1 Rep 2 28 35 22 17 18 2 8 Rep 3 1 1 1 1 1 1 1 Rep 3 19 31 31 23 21 10 10 Rep 4 1 1 1 1 1 1 1 Rep 4 14 27 25 27 16 13 11 RepS 1 1 1 1 1 1 1 RepS 17 32 25 22 17 10 11 Rep 6 1 1 1 1 1 0 1 RepS 13 34 27 20 7 0 5 Rep 7 0 1 1 1 1 1 1 Rep 7 1 29 21 19 13 14 10 RepS 1 1 1 1 1 1 1 Rep 8 18 33 28 17 15 6 2 Rep 9 1 1 1 1 1 1 1 Rep 9 10 30 30 25 10 5 0 Rep 10 1 1 1 1 1 1 1 Rep 10 23 34 27 23 10 8 3 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:43(p1 of 1) 12-9976-2955/0802-S056C Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 05-9844-4642 Endpolnt: 02 Apr-08 1 1 :43 Analysis: 7d Survival Rate CETIS Version: CETISv1.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% TestStat P-Value Declslon(0.05) Lab Control 6.25 1 12.5 1 25 1 50 0.237 100 1 Test Acceptability Attribute Test Stat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- M OJ- f 0.7- | 0.6- 3 o.s- S o., 0.3-i 0.2- 0.1- 0.0 Lab Control 10 0 10 0 10 0 10 0 8 2 10 0 • • • e 0 6.25 12.S 25 Conc-% 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 10 10 • • 50 100 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:43(p1 of 1) 12-9976-2955/0802-S056C Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 18-4393-2691 Analyzed: 02 Apr-OS 1 1 :43 Endpolnt: Reproduction Analysis: Parametric-Control vs Treatments Data Transform Zeta Aft Hyp Untransformed OT Dunnett's Multiple Comparison Test Control vs Conc-% TestStat Lab Control 6.25* 12.5' 25* 50* 100* Test Acceptability Attribute Control Resp PMSD 3.41 6.24 10.4 14.1 14.9 Monte Carlo NOEL Not Run <6.25 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes LOEL TOEL TU PMSD 6.25 N/A 12.3% Critical MSD P-Value Decision(S%) 2.29 3.89 0.0028 2.29 3.89 0.0000 2.29 3.89 0.0000 2.29 3.89 0.0000 2.29 3.89 0.0000 Significant Effect Significant Effect Significant Effect Significant Effect Significant Effect TestStat Acceptability Limits Overlap Decision 31.6 15 -NL Yes 0.123 0.13-0.47 Yes ANOVA Table Source Sum Squares Mean Square Between 5141.083 Error 779.1 Total 5920.184 ANOVA Assumptions Attribute Test 1028.217 14.42778 1042.644 Passes acceptability criteria Fails acceptability criteria OF FStat P-Value Decision(5%) 5 71.3 0.0000 54 59 Significant Effect TestStat Critical P-Value Decision(1%) Variances BarUett Equality of Variance Distribution Shapiro-Wifk Normality Reproduction Summary Conc-% Control Type Count Mean 0 Lab Control 10 6.25 10 12.5 10 25 10 50 10 100 10 Graphics 50- 40- § 5 -•- I 3°"a ~*~ 20- * 10- 31.6 25.8 21 13.9 7.7 6.3 3.53 15.1 0.6180 0.977 0.3000 95%LCL 95%UCL Min 30.6 32.6 27 24.5 27.1 21 19.6 22.4 17 12.3 15.5 7 5.99 9.41 0 4.72 7.88 0 8- 6- 1 *: !H ReJectNuU 0| Q: -2: F n "*1 * Equal Variances Normal Distribution Max StdErr Std Dev CV% Diff% 35 0.465 2.5 7.92% 0.0% 31 0.636 3.43 13.3% 18.4% 27 0.661 3.56 16.9% 33.5% 21 0.795 4.28 30.8% 56.0% 14 0.835 4.5 58.4% 75.6% 11 0.773 4.16 66.1% 80.1% S+ /*/_m • / 0 6.25 12.5 25 50 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Conc-% Rankits 000-089-163-3 CETIS ™v1.6.3revE Analyst; CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:43(p1 of 1) 12-9976-2955/0802-S056C Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 06-9939-2046 Analyzed: 02 Apr-08 11:43 Endpoint Reproduction Analysis: Linear Interpolation (ICPIN) CETIS Version: Official Results: CETJSv1.6.3 Yes Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 95% CL Method Linear Linear 5795186 280 Yes Two-Point Interpolation Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp 31.6 15-NL Yes Passes acceptability criteria Point Estimates Effect-% Conc-% 95%LCL 95%UCL 50 21.7 18.8 28.1 Reproduction Summary Conc-% Control Type Calculated Variate Count Mean Win Max Std Err Std Dev CV%Diff% 0 6.25 12.5 25 50 100 Lab Control 10 10 10 10 10 10 31.6 25.8 21 13.9 7.7 6.3 27 21 17 7 0 0 35 31 27 21 14 11 0.457 0.625 0.65 0.781 0.821 0.76 2.5 3.43 3.56 4.28 4.5 4.16 7.92% 13.3% 16.9% 30.8% 58.4% 66.1% 0.0% 18.4% 33.5% 56.0% 75.6% 80.1% Reproduction Detail Conc-% Control Type Rep1 Rep 2 Rep 3 Rep 4 RepS Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control 31 22 17 12 9 3 35 22 17 18 2 8 31 31 23 21 10 10 27 25 27 16 13 11 32 25 22 17 10 11 34 27 20 7 0 5 29 21 19 13 14 10 33 28 17 15 6 2 30 30 25 10 5 0 34 27 23 10 8 3 Graphics 35- 30- 20- 20 40 60 BO 100 120 000-089-163-3 CETIS™ v1.6.3revE Analyst:QA: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:44<p1 of 1) 12-9976-2955/0802-S056C Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 12-3920-9398 Endpoint: Reproduction Analyzed: 02 Apr-08 11:44 Analysis: Parametric-Two Sample Data Transform Untransformed Zeta Alt Hyp Monte Carlo NOEL C > T Not Run <0 Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Lab Control Conductivity Control 6.38 1.73 4.51 0.0000 Test Acceptability Attribute Control Resp Control Resp PMSD TestStat Acceptability Limits Overlap 31.6 15 -NL Yes 15 15 -NL Yes 0.143 0.13-0.47 Yes ANOVA Table Source Sum Squares Mean Square DF FStat P-Value Between 1377.8 1377.8 1 40.8 0.0000 Error 608.4 33.8 18 Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 14.3% Decision(5%) Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Total 1986.2 1411.6 19 ANOVA Assumptions Attribute Test TestStat Critical P-Value Variances Variance Ratio F 9.79 6.54 0.0023 Distribution Shapiro-Wilk Normality 0.958 0.5120 Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95%UCL Min 0 Lab Control 10 31.6 30.6 32.6 27 0 Conductivity Co 10 15 12 18 1 Graphics 5°: 40- 2fr CoJ 15- Ifr ll ^ 1 I •_,. ~5~ Reject Null ,10_ Decision{1%) Unequal Variances Normal Distribution Max StdErr StdDev CV% Diff% 35 0.465 2.5 7.92% 0.0% 28 1.45 7.83 52.2% 52.5% S / 0 0 -2.0 -1.S -1.0 -0.5 0.0 0.5 1.0 15 2.0 tt&WT* conc-% {JSJ.D Ranklts 000-089-163-3 CETIS™ v1.6.3revE Analys :reshwater Chronic Bioassay Client/Sample ID: Dudek Carlsbad/Well #1 Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: End Date/Time: (4)(7L 2/12/2008 2/19/2008 1Q30 Cone. LC Rep 1 2 3 4 5 6 7 8 9id Analyst Verified by: Board/ Cup U9 \ f(o iq > >• ?£- M *&l $\ &~- *H3 Daily Reproduction/ Survival :::::::&:!;;:: 0oo ?oo 00 O C» 0 t^A- |;!:i:I«?&j::: 1? rt 2?c> /) J5 c.'/> /\ O fli- •:.?£&}:$'f O Or & 6 0O O <2o0 £<r ':::3il-:::;:: 0 <5 I /^ C, fZ -)f) & <0 OVX/ :j:j:j!Sii;;i:: 5 ' O c^ O O O Q t-5 o AO Si-:! 8i:!:!:i fl ' ^1i 13 L-&. 1 ^5 IP ft ^ It" \/L J°Vu V^Jr i:|:|:|3[;|:l:|i S' ** Ifj ]U iltf •70u 15i<5 .NH ;j£i:$;j;;:| i . j , , . Total 3135 ^} 3-13*2.3«f i^-??^o 34 QC l"2- Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival „ *1i*;:; 5oo o 0ooooo ;*;:;?!:=:;: /o O O 0 0jy 0 0 B 0 mmmo 6 00oo 0e oo :ii:Hi#;!;!; 0 fo s o 2- *// (9 H 3*5!!::!!: ^ ^U- f> .3 ^—> / A i!#;8i!!#! I- ^—\3 * ;;i;i:!7)|;^; 0 ib ? 3-fJf >r? ;!;;;!«;;;;;! — Total I 7 1 $•!/A l« 10 ^3 Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival #&!*! & ft r> O 6 fc C Oo r> l^hlZi^i; O 0 ebo 0 0& 0^ ;!#H3:;#! o C? C? O O ft Oo&o i:;:;;;4iii:;; 2_ ? $ 3 ^5 j~ 3 -7 d :=!!i!!$!N!|itfifeoI«L ^£> O / P 1 1 i;i;;;:8l;;i!|; 4<vf>7n»^ Mib</ !#|;i7:-;::;; ||t4i (?•H11y.f'4-L7-li^ |;!!i!Wj!!iii Total ol69~ c3ia- 31 ^5 ^•3-7 2- ^?V2n Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival . . lil&JSI 0 O O c>oo oc5 <?o iiiiliaiHll!: 0 o 0 O 0c 0 o o o mmm o/»o Q O oO A 0o !ii==!i«;i!l! \ H &sr 4 >5 •2- 3 4 6 *!£#;*! t> O Oaio 0 0o 0 •nnH*!;!H;i ^.3A5«r•* Zry •L^5 ;!!!!!pl=il?16o I U *ftij ,T/& (2- !ii;!ij?!;i!iii Total n It *i413»>- fi7 1*1nvs. 3A QC ^ QC \«4 QC y me Fed/Test Solution Renewed (day): lotes: d = dead; M = male; LIP = tost in progress; B = 4th brood (only the first 3 broods are included in total) omments: >C Check:Final Review: Jautilus Environmental 5550 Morehouse Drive, Suite 1 SO. San Diego, C4 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek Carlsbad/Well #1 Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: End Date/Time: 2/12/2008 2/19/2008 'lO?>0 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup to j )Hb W ^l P^ ^H Ml ^5H bi. Sfr Daily Reproduction/ Survival lii&HHSN Ou 0 0 0 0 o 0 0o PA- jijlj^lii?!!s « o o O O C 0 C' 0 A/i-r !!iHll«!=!=!! 0e> 0 o O 6 0 0 o o e$ £!i|!4iiH!i! O 2-<r ^ o o \ o o o t>W iiiiiilSiii **23 tJ O / O O «Q '^4.4o !|=h!«!!l!=! 'i?3t n^5 0«?- s5 7 'ft"V^ fiiiliusiiiiii f(( IV 5T ^'T- |O (0 Oo »^A- HHiJSlilil! -1 ]—3 « Total 11 \S -a-i l<^n7 \b ft |0 P QC i Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survivalmmoco Cif>r; ft on 0 vmmc c ft 0 0 O<? o o> 0 wmm C, */Ae> o o (7 m^oo o c? O— 3 t9 On JL ^-MJ) M n o *tfX-ji^ !l!=!ii5iii!i!i!=f__ >4u \\ *L 3& iiiiiiiSiiiii! _ — Total 1,aM |0 13i<? o/A 14 & 5"8 QC lO Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 t> O 0 Co cn Oou •• *o o 0 0I) 00 0 0 D $ \ 0 oo o o <y O 0 0 <3 4 . <5 0 0 0 £> 0 <9 0 O o 5 <f> 0 0 O o£> C? G rt G $ *>5!? ^5" "i3 (0 •H <?- ^si T D <5 5 -=?- ^?5<*oo 0 $Total 3 «5 ICP i<n 5 10&x O 2) QC O Time Fed/Test Solution Renewed (day): (0) 16/S miX(S (21 \>05 (3)_ Notes: d = dead; M = male; LIP = tost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Check: Iff Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client: Oudek Carlsbad Sample ID: Well #1 Test No: Water Quality Measurements Test Species: C. dubla Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 Concentration Da PH DO <mg/L) Cond. {(jmhos/cm) Temp (°C) Conductivity Control 7.M2, PH DO (mg/L) Temp (*C) 2-4, lfiffrlfn <*. 01 1.3 "14*. O as .7 Concentration Da PH DO (mg/L) Cond. (pmhos/cm) Temp ('C) 6.25% °. r 1' 1.213 ir.o 2-1,7 7,5- PH DO (mg/L) Temp CO .• 1 1 JwL X.3as. 7 Concentration Day 12.5% 1... '. 2 .[...» PH DO (mg/L) Cond. (pmnos/cm) Temp ° Zfct Sot PH DO (mg/L) Temp (°C) * 7. 8.JM §-,0 ^ n 2-5V? Analysts: Initial: Final: Sample Used (A, B. C): Comments: Animal Source/Date Received: Sample Log-in Numbers: A: QC Check: W e-5 PP iA- . t r B: Animal Age at Initiation: <£ ffiVJIf'". C: M/A Final Review: Nautilus Environmental 5550 Morehouse Drive, Sorte f 50. San Diego, CM 92J21. Freshwater Chronic Bioassay Water Quality Measurements Client: _ Dudek Carlsbad Sample ID: \\XAl ^ Test No: Test Species: C. dubia Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 jt) 2)0 Concentration Da 25% 1 PH DO (mg/L) Cond. (pmhos/cm) Temp ("C) L-L^-1/ \: ,?m 7. M -752, 7.6,3 2.5. 1 •1.63 ->.. -M.3 PH DO (mg/L) Temp ('C) a-IJ is- y Concentration Da 50% PH DO (mg/L) Cond. (iimhos/cm) Temp (°C) 7.0 (3(0 2-5. PH DO (mgIL) Temp^C)55. J -72^3 "7.3 Concentration Day 1...... !......« i 3 1 4 PH DO (mg/L) Cond. (pmhos/cm) Temp °C) 7. II PH DO (mg/L) Temp (°CJ =25-3 Analysts: Initial: Final: Sample Used (A, B, C): £5 A A df A Comments: Animal Source/Date Received: _ Sample Log-in Numbers: A: QC Check: r^f -7 95.7 B: Animal Age at Initiation: C: N)//V Final Review: Nautilus Environmental 5550 Morehousa Drive, Suite 150. San Diego. CA 92121. Site: Well 3 CETIS Summary Report Report Date: Link/Link Code: 02Apr-0811:36(p1 of 2) 05-5297-5731/0802-S056d Ceriodaphnla 7-d Survival and Reproduction Test Test Run No: 10-8130-8265 Test Type: Reproduction-Survival (7d) Start Date: 12 Feb-08 15:35 Protocol: EPA/821/R-02-013 (2002) Ending Date: 19 Feb-08 10:55 Species: Cerlodaphnia dubia Duration: 6d 19h Source: In-House Culture Sample No: 10-6887-8387 Code: 08-0169 Sample Date: 11 Feb-08 12:30 Material: Groundwater Receive Date: 12 Feb-08 14:30 Source: Dudek & Associates Sample Age: 27h(5.9°C) Station: Well 3 Comparison Summary Analysis No Endpoint 1 5-383 1 -3399 7d Survival Rate 20-8801-0022 Reproduction Point Estimate Summary Analysis No Endpoint 17-7586-9391 7d Survival Rate 06-3437-3206 Reproduction Test Acceptability Analysis No Endpoint 15-3831-3399 7d Survival Rate 17-7586-9391 7d Survival Rate 06-3437-3206 Reproduction 20-8801-0022 Reproduction 20-8801-0022 Reproduction NOEL 50 12.5 Effect-% 50 50 Attribute Control Resp Control Resp Control Resp Control Resp PMSD LOEL 100 25 Conc-% 68.9 27.5 TestStat 1 1 27.1 27.1 0.142 TOEL 70.7 17.7 Nautilus Environmental (CA) Analyst Diluent Diluted Mineral Water (8:2) Brine: Not Applicable Age: <8h Client Dudek & Associates Project: PMSD N/A 14.2% Method Fisher Exact/Bonferroni-Holm Test Wilcoxon/Bonferroni Adj Test 95% LCL 95% UCL Method 48.7 22.9 127 34.9 Linear Regression (MLE) Linear Interpolation (ICPIN) Acceptabilty Limits Overlap 0.8 - NL 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Yes Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 9 9 9 10 10 9 Mean 0.7 1 1 1 0.9 0.8 0.222 95% LCL 0.52 1 1 1 0.782 0.643 0.0576 95% UCL 0.88 1 1 1 1 0.957 0.387 Min 0 1 1 1 0 0 0 Max 1 1 1 1 1 1 1 Std Err 0.0882 0 0 0 0.0577 0.077 0.0805 Std Dev 0.483 0 0 0 0.316 0.422 0.441 CV% 69.0% 0.0% 0.0% 0.0% 35.1% 52.7% 198.0% Dl«% 0.0% -42.9% -42.9% -42.9% -28.6% -14.3% 68.3% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 9 9 9 10 10 9 Mean 1.2 27.1 22.9 24.3 14.2 7.8 0 95% LCL 0.57 25.9 21.2 23.6 12.6 6.37 0 95% UCL 1.83 28.3 24.6 25.1 15.8 9.23 0 Min 0 21 14 22 5 2 0 Max 4 32 28 27 20 14 0 Std Err 0.308 0.602 0.839 0.365 0.769 0.698 0 Std Dev 1.69 3.3 4.59 2 4.21 3.82 0 cv% 141.0% 12.2% 20.1% 8.22% 29.7% 49.0% Diff% 0.0% -2160.0% -1810.0% -1930.0% -1080.0% -550.0% 100.0% 000-089-163-3 CETIS™ v1.6.3revE Analyst:QA:7 CETIS Summary Report Report Date: Link/Link Code: 02Apr-0811:36(p2of 2) 05-5297-5731/0802-S056d Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 0 Rep 2 1 1 1 1 1 1 0 Rep 3 1 1 1 1 1 0 1 Rep 4 0 1 1 1 1 1 0 RepS 0 1 1 0 0 Rep 6 1 1 1 1 1 1 Rep7 1 1 1 1 1 1 0 RepS 1 1 1 1 1 1 1 Rep 9 1 1 1 1 1 1 0 Rep 10 0 1 1 1 0 1 0 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 2 27 26 20 14 0 Rep 2 0 26 23 27 12 3 0 Rep 3 4 30 18 25 12 8 0 Rep 4 0 29 26 22 14 8 0 RepS 0 27 13 9 0 Rep 6 0 25 14 27 15 8 Rep 7 4 32 21 22 15 2 0 RepS 0 21 28 23 18 5 0 Rep 9 2 25 26 23 18 13 0 Rep 10 0 29 23 24 5 8 0 000-089-163-3 CETIS™ v1.6.3revE AnalvstAS NA QA:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p1of 1) 05-5297-5731/0802-S056d Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 15-3831-3399 Endpoint 02 Apr-OS 11 :35 Analysis: 7d Survival Rate CETIS Version: CETISv1.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 50 100 70.7 2 N/A Fisher Exact/Bonferronl-Holm Test Control vs Conc-% TestStat P-Value Oecision(O.OS) Lab Control 6.25 1 1 Non-Significant Effect 12.5 1 1 Non-Significant Effect 25 0.526 1 Non-Significant Effect 50 0.263 1 Non-Significant Effect 100 0.00113 0.00566 Significant Effect Test Acceptability Attribute Test Slat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- 5 "•°« 0.7- •I 0.6- 3 0.5- 5 „.. O.J- 0.2- O.lr 0.0 Lab Control 9 0 9 0 9 0 9 1 a 2 2 7 Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 9 9 9 10 10 9 • e • • • • 0 6.25 12.5 25 Conc-% 50 tOO 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p1of 2) 05-5297-5731/0802-S056d Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 17-7586-9391 Endpolnt 7d Survival Rate Analyzed: 02 Apr-08 11:35 Analysis: Linear Regression (MLE) Linear Regression Options Model Function Threshold Option Log-Normal [NED=A+B*log(X)] Regression Summary Iters LL AlCc 4 -13.6 37.2 Mu -0.474 Control Threshold Sigma 0.273 GStat 0.385 CETIS Version: Official Results Threshold Optimized Pooled 0 Chi-Sq 1.12 Yes Critical 7.81 Yes P-Value 0.7730 Het Con- No Nautilus Environmental (CA) CETISv1.6.3 : Yes Weighted Yes Decision(6%) Non-Significant Heterogeneity Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 68.9 48.7 127 Test Acceptability Attribute Control Resp Test Stat Acceptability 1 Limits 0.8 - NL Regression Parameters Parameter Estimate Std Error 95% LCL 95% UCL tStat Slope 3.67 Intercept -1 .74 Residual Analysis Attribute Method 1.16 2.04 1.39 -5.75 5.94 2.27 3.16 -0.851 Test Stat Critical Distribution Shapiro-Wilk Normality 7d Survival Rate Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 7d Survival Rate Detail Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 0.983 Overlap Yes P-Value 0.0508 0.4580 P-Value 0.9480 Decision Passes acceptability criteria Decision(5%) Non-Significant Parameter Non-Significant Parameter Decision(5%) Normal Distribution Calculated Variate(A/B) Count 9 9 9 10 10 9 Repf 1 1 1 1 1 0 Mean 1 1 1 0.9 0.8 0.222 Rep 2 1 1 1 1 1 0 Win 1 1 1 0 0 0 Rep 3 1 1 1 1 0 1 Max 1 1 1 1 1 1 Rep 4 1 1 1 1 1 0 Std Err 0 0 0 0.0577 0.077 0.0805 Rep 5 1 1 1 1 0 0 Std Dev 0 0 0 0.316 0.422 0.441 Rep 6 1 1 1 1 1 0 CV% 0.0% 0.0% 0.0% 35.1% 52.7% 198.0% Rep 7 1 1 1 1 1 1 Diff% 0.0% 0.0% 0.0% 10.0% 20.0% 77.8% Rep 8 1 1 1 1 1 0 A 9 9 9 9 8 2 Rep 9 1 1 1 1 1 0 B 9 9 9 10 10 9 Rep 10 0 1 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p2of 2) 05-5297-5731/0802-S056d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-7586-9391 Analyzed: 02 Apr-OS 11:35 Endpoint: 7d Survival Rate Analysis: Linear Regression (MLE) CETIS Version: CETISv1.6.3 Official Results: Yes Graphics 0.8- 0.6- 0.4- 0-2- 0.0 •0.2- -0.4- -0.6- 100 120 -O.ft O.fr 0.6- 0.1: 0.2- 0.0! -0.2- -0.* •0.6- -0.8- 0* 0.6- O.'h 0.2- 0.0 -0.2: -0.4- 20 40 60 Conc-% 100 120 -O* -1.0 -0.5 0.0 RanUtf O.S 1.0 1.S 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 7d Survival Rate 000-089-163-3 CETIS I"v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p1of 2) 05-5297-5731/0802-S056d Ceriodaphnla 7-d Survival and Reproduction Test Analysis No: Analyzed: 20-8801-0022 Endpoint: Reproduction 02 Apr-OS 11:36 Analysis: Nonparametric-Multiple Comparison Data Transform Rank Zeta Wilcoxon/Bonferroni Adj Test Control vs Cortc-% Lab Control 6.25 12.5 25* 50* 100* Test Acceptability Attribute Control Resp PMSD ANOVA Table Source Between Error Total Alt Hyp OT Test Stat 64 64.5 55 55 45 Monte Carlo Not Run Critical Test Stat Acceptability Ties 3 3 0 0 0 Limits 27.1 15 -NL 0.142 0.13-0.47 Sum Squares 5171.147 578.9778 5750.125 Mean Square 1034.229 11.57956 1045.809 ANOVA Assumptions Attribute Test Variances Distribution Mod Levene Equality of Varianc Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type 0 6.25 12.5 25 50 100 Lab Control Rank Transformed Summary Conc-% Control Type 0 6.25 12.5 25 50 100 Lab Control Count 9 9 ' 9 10 10 9 Count 9 9 9 10 10 9 Mean 27.1 22.9 24.3 14.2 7.8 0 Mean 47.7 39.2 41.2 23.9 15.9 5 DF 5 50 55 Test Stat 4.44 0.948 95% LCL 25.9 21.1 23.6 12.6 6.35 0 96% LCL 44.7 35.6 38.9 21.7 14.2 5 FStat 89.3 Critical 3.41 95% UCL 28.4 24.6 25.1 15.8 9.25 0 95% UCL 50.7 42.9 43.4 26.1 17.6 5 NOEL 12.5 P-Value 0.1250 0.1250 0.0000 0.0000 0.0001 Overlap Yes Yes P-Value 0.0000 P-Value 0.0020 0.0168 Min 21 14 22 5 2 0 Min 32.5 24 34.5 12.5 10 5 Nautilus Environmental (CA) CETIS Version: CETISvl Official Results: Yes LOEL 25 TOEL 17.7 TU 8 .6.3 PMSD 14.2% Oecision(5%) Non-Significant Effect Non-Significant Effect Significant Effect Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Decision(1%) Unequal Variances Normal Distribution Max 32 28 27 20 14 0 Max 56 52 49.5 31 24 5 StdErr 0.612 0.853 0.371 0.782 0.71 0 StdErr 1.46 1.78 1.09 1.05 0.813 0 Std Dev 3.3 4.59 2 4.21 3.82 0 Std Dev 7.85 9.59 5.87 5.68 4.38 0 CV% 12.2% 20.1% 8.22% 29.7% 49.0% CV% 16.5% 24.4% 14.3% 23.8% 27.5% 0.0% Diff% 0.0% 15.6% 10.2% 47.6% 71.2% 100.0% Diff% 0.0% 17.8% 13.7% 49.9% 66.7% 89.5% 000-089-163-3 CETIS™ v1.6.3revE Analyst CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p2of 2) 05-5297-5731 /0802-S056d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 20-8301-0022 Endpoint Reproduction Analyzed: 02 Apr-OS 11:36 Analysis: Nonparametric-Multlple Comparison CETIS Version: CETISvl .6.3 Official Results: Yes Graphics I 30- 20- 10- 15- 10- 5- 0 -5- -10: -15- 6.25 12.5 25 Conc-°/o 50 100 -20- -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Ranktts 000-089-163-3 CETIS™ v1.6.3revE Analyst: ^<^^A QA:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:36(p1of 1) 05-5297-5731/0802-S056d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 06-3437-3206 Analyzed: 02 Apr-OS 11:36 Endpoint Reproduction Analysis: Linear Interpolation (ICPIN) CETIS Version: Official Results: CETISvl.6.3 Yes Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 96% CL Method Linear Linear 7055475 280 Yes Two-Point Interpolation Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp 27.1 15-NL Yes Passes acceptability criteria Point Estimates Effect-% Conc-%95% LCL 95% UCL 50 27.5 22.9 34.9 Reproduction Summary Conc-% Control Type Calculated Variate Count Mean Min Max Std Err Std Dev CV%Dlff% 0 6.25 12.5 25 50 100 Lab Control 9 9 9 10 10 9 27.1 22.9 24.3 14.2 7.8 0 21 14 22 5 2 0 32 28 27 20 14 0 0.602 0.839 0.365 0.769 0.698 0 3.3 4.59 2 4.21 3.82 0 12.2% 20.1% 8.22% 29.7% 49.0% 0.0% 15.6% 10.2% 47.6% 71.2% 100.0% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control 27 23 26 20 14 0 26 18 27 12 3 0 30 26 25 12 8 0 29 27 22 14 8 0 25 14 27 13 9 0 32 21 22 15 8 0 21 28 23 15 2 0 25 26 23 18 5 0 29 23 24 18 13 0 Graphics 100 120 000^)89-163-3 CETIS™ v1.6.3revE Analyst: CETIS Analytical Report VK Report Date: Link/Unk Code: 02Apr-0811:37(p1of 1) 05-5297-5731/0802-S056d Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 07-8078-6203 Endpoint: 7d Survival Rate Analyzed: 02 Apr-08 11:36 Analysis: Single 2x2 Contingency Table CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run >0 N/A N/A Fisher Exact Test Control vs Control TestStat P-Value Oecision(0.05) Lab Control Conductivity Control 0.124 0.124 Non-Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp Control Resp 1 0.7 0.8 - NL 0.8 - NL Yes Passes acceptability criteria Yes Fails acceptability criteria Data Summary Conc-% Control Type No-Resp Resp Total Conductivity Co 7 Lab Control 9 3 0 10 9 Graphics 1.0-. 0.9- 0.8- 0.7- 0.6 0.5- 0.4- 0.3: 0.2- 0.1- 0.0 COIK-% 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:37(p1of 1) 05-5297-5731/0802-S056d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 21-0424-5507 Endpoint: Reproduction Analyzed: 02 Apr-OS 11:36 Analysis: Parametric-Two Sample Data Transform Zeta Alt Hyp Untransformed C > T Equal Variance t Two-Sample Test Control vs Control TestStat Lab Control Conductivity Control 21 .9 Monte Carlo NOEL Not Run <0 CETIS Version: CETISvl.6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 7.58% Critical MSD P-Value Decision(5%) 1.74 2.06 0.0000 Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp 27.1 15 - NL Yes Control Resp 1.2 15 -NL Yes PMSD 0.0758 0.13-0.47 Yes ANOVA Table Source Sum Squares Mean Square Between 3180.248 3180.248 Error 112.4889 6.616993 Total 3292.737 3186.865 ANOVA Assumptions Attribute Test Variances Variance Ratio F Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count Mean 0 Lab Control 9 27.1 0 Conductivity Co 10 1.2 Graphics SO-, 40- c •9 30- 0. « 20- 10- 1 » 1 Passes acceptability criteria Fails acceptability criteria Fails acceptability criteria DF FStat P-Value Oecision(S%) 1 481 0.0000 17 18 Significant Effect TestStat Critical P-Value Decision! 1%) 3.82 6.69 0.0620 0.93 0.1710 95%LCL 95%UCL Min 25.9 28.4 21 0.558 1.84 0 6-j «: IH 3 -2- -»: -6: Equal Variances Normal Distribution Max StdErr StdDev CV% 32 0.612 3.3 12.2% 4 0.313 1.69 141.0% Diff% 0.0% 95.6% • »^» 9f^"^ • •^••» • •s' • o i i -o i1 r r -"T 1 1 .0 0 . -2.0 -15 -1.0 -0.5 0.0 O.S 1.0 1.5 tVJUM* conc.% /jab R^Wt, 'I 2.0 000-089-163-3 CETIS™ v1.6.3revE Analyst: Freshwater Chronic Bioassay Client/Sample ID: Dudek Carlsbad/Well #3 "Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: End Date/Time: 2/12/2008 2/19/2008 (056 Cone. LC Rep 1 2 3 4 5 B 7 8 9 10 Analyst Verified by: Board/ Cupi« <JJ \ H(f H •31olM ,^ Hi fit£« yfv <?G- Daily Reproduction/ Survival HiiNJIIiilii! £? <?6 oo 0 6 tljC O N«r ££;*£=!! O 0 O G> C-1 aS ?6 O M IHliflSHg!0 Aof) 0o 0 0 0 0 /M !i;=;!iif!!H!l 2-•? (, H /. 5 62. 1 5 <W !iiij;j3!:;# £> <9 O O {? O <9 0 p O •tft' i:iij;S;j#;: t*>7£ IJj3 te1$7 .1l-i- Kf :i:;:i:7i:;:;i-is ,!if5!HUP W{?A7- tlx Nfr ^!:|«;;;:!; — Total oc-j -2£* 3c? 2^1 U//Mf a^3?- Q2fi ^\ QC li Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival j!:!j!!;;!j::i b O c)ooO t> 0 G C> m&m6c 0<•) ot>o^) 0 MO :|:i:!!i5;iH::o 0o 1(4<pD O 0 0— •- iOl;i#*i! c? e> a— e/<rf6o 0 <9i ;|!!!|!5i!!i!!: i^ 0 1. -. — — rt YAT) 0 — - *£»*;;!raft A. _ . — (O& 0 Q ~~ IHilliJ!*;* t? O <5 — Oo D 2- — iijiliiPi'li — — . — Total 1~ O 1^0/4 O/A O 1d -2- ,&/A. Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival |i|:l1:ii|i!i £, & O 00 o Doa v * \i\mm\ r^o oo 0 0 d 0 e D !i!!;j;3;i!l!! O o 0 0 0 0 0 0o 0 inilllShiji! 3 3 Z_ H S~ •\ 3 S~ 5~ 4 mmx 6 0 O £> O O d> 6 3 o *:\mm ^-i 5_Yfo2L to \OV7 :;:;:;=?;:;;;:; UP li (^M- > ffiii?> 1^k- ;:i;;;i8.ii::;;: T Total KIl+'P 31> i«8 2^> •?1T-t 4-$ IM) 3.1 QC a. QC 15. Cone 12.5% Rep 1 2 3 4 5 6 7 8 9 ; 10 Dally Reproduction/ Survival iiii&iiiil: G> <*) e^> A 0 0 0 0 O £li!*8h!i c 8o o D 0eec 0 IS*?;!*; 0 rt (? ^(5 0oo o 0 !*;;!##!;f 21 s?\ 5£i> 1 !!li!il5ii:i!i6 & 1 0oo 0 0 0o i!!|ijlSp;;i ^•7- ^4/^ 7fry Q*7 *t hP^TIP;;it-if? 42^ IOf | GVA _L p' u :;;;;:i8!;>;H — - Total ^i? ^1.°y£>£uS*~ \\/i# ^1^LA J3 4,3j«-r QC IdL rime Fed/Test Solution Renewed (day):_ ^ .(3).(8) Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Check:Ef Final Review: Nautilus environmental 5550 Morehouso Drive, Suite 150. San 0/090, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Dudek Carlsbad/Well #3 Start Date/Time: End Date/Time: 2/12/2008 2/19/2008 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup 10 t i va \ H \p \4 ?~\ Sf-\ <\fftj-i 3\ 53 ,»•" 16- Daily Reproduction/ Survival !!i|£ii!&! r> r> o O O Cj O(<~) 0 D (MA ;;;;ii^!;ii;:; O 0 0 e> vi 0 0 0 0 0 A/U |;:ii;gi|:i;i; 0o 0 O 0 0 0 0 0 0 W &M& •^t «^ ;i;iii;S:;;l;i O 0 0 0 0 0 p o 1 4£ iiiii;i^i|l|i! 7^3 v^ U t^L- xJS'7£? IT —taF i:;i;i!?H!iH 6 .3 5 2 ^7- 9- gf? ^^. MA 1i!i;i8£!i! .. Total JD r2* pfHTri H 15 18 £"M QC 7 Cone.Rep Daily Reproduction/ Survival Total QC 50%0 0 t?±O * 10 o 0 5 ) O D 0 0 O o o 6?O Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival !!!*!!!H! •e> O Oo Q (^ Q 0 O 0 iHliHailh!! £> O 0 gy^.<~*t/6 o 0 0 0 0 0 d 0 D O 0 !=!ij!i*!ij!!! 0 oo°/^ 6 — 0 o o 0 $!=!*;!!!!! o o 0 — 0 —til A 0 *>,o/d !i!!j!l«i!!!!i O 0/ct 0— cV<?C- -^ —O o/^C — illiiii^iiHl! p/D— o— • — _ o — — !!il!ll*!!!l!l —— — — — -- — Total ^/4 c/A *?JoMo/a —-0/4! 0 f»«L 0/d QC O Time Fed/Test Solution Renewed (day): Notes:d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) QC Check:Final Review: Nautilus Environmental $550 Morehouse Drive, Suite 150. San 0/ego, CA 92121. Freshwater Chronic Bioassay Client:Dudek Carlsbad Sample ID: Well #3 Test No: Water Quality Measurements Test Species: C. duo/a Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 Concentration Da PH DO (mn/L) Cond. (pmhos/cm) Tem °C 6.25% JLJL PH DO (mg/L) Temp (*C) M- 7,4 ft. •2.M-.1 Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: Sample Log-in Numbers: QC Check: #= .A 65 A of A A P A A:B: Animal Age at Initiation: A- C: feJIrY Final Review: Nautilus Environmental 5550 Moreftouse Drive. Suite f 50. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client: _ Oudek Carlsbad Sample ID: W-t\\ Test No: CgQ3-<<: e Test Species: C. dubia Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 Concentration 25% Day 1 I ». PH DO (mg/L)1-7 Cond. (pmhos/cm) Temp CO IS. 2-2.M.4 -7.0 PH DO (mg/L) Temp CO 2-M.7 23.3 ^5.7 Concentration 50% Da PH DO (mg/L) Cond. (pmhos/cm) Temp ("C) -7.IH PH DO (mgrt.) Temp (*C)2X.7 as.j -?, o *»•(? Concentration 100% Da PH DO (mg/L) Cond. (pmhos/cm) Temp CO to C.4I 2M.7 -7.0$.n 4886 PH DO {mg/L) Temp (°C)55T. A £5 A Of3 Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: Sample Log-in Numbers: A:Cffb& —D QC Check: ££ Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. (2-5 A A Animal Age at Initiation: B:; MA- Final Review: Site: Surface 1 CETIS Summary Report Report Date: LlnK/Link Code: 04Apr-0816:31 (p1 of 2) 20-8424-2555/0802-S056a Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: Start Date: Ending Date: Duration: Sample No: Sample Data: Receive Date: Sample Age: 00-2685-6779 Test Type: Reproduction-Survival (7d) 12 Feb-08 15:55 Protocol: EPA/821/R-02-013 (2002) 19Feb-0809:25 Species: Ceriodaphnia dubia 6d 17h Source: In-House Culture 02-5959-5179 Code: 08-0166 11 Feb-08 13:45 Material: Receiving Water 12 Feb-08 14:30 Source: Dudek & Associates 26h (6 *C) Station: Surface 1 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <8h Client Dudek & Associates Project Comparison Summary Analysis No 17-3527-8295 13-6665-8603 Endpoint 7d Survival Rate Reproduction NOEL LOEL 100 >100 50 100 TOEL N/A 70.7 PMSD N/A 21.2% Method Fisher Exact/Bonferroni-Holm Test BonferroniAdjtTest Point Estimate Summary Analysis No 10-2843-5091 Endpoint Reproduction Effect-% 50 Conc-% 79.2 95% LCL 69.5 95% UCL 88.4 Method Linear Interpolation (ICPIN) Test Acceptability Analysis No 17-3527-8295 10-2843-5091 13-6665-8603 13-6665-8603 Endpoint Attribute 7d Survival Rate Reproduction Reproduction Reproduction Control Resp Control Resp Control PMSD Resp TestStat 1 27.1 27.1 0.212 Acceptabilty Limits 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Overlap Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 9 9 Mean 0.7 1 1 1 1 1 1 95% LCL 0.52 1 1 1 1 1 1 95% UCL 0.88 1 1 1 1 1 1 Min 0 1 1 1 1 1 1 Max 1 1 1 1 1 1 1 StdErr 0.0882 0 0 0 0 0 0 StdDev 0.483 0 0 0 0 0 0 CV% 69.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% Diff% 0.0% -42.9% -42.9% -42.9% -42.9% -42.9% -42.9% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 9 9 Mean 9.9 27.1 27.2 26.7 25 22.3 7.33 95% LCL 6.69 24.6 25.8 25.5 23.3 19.7 5.38 95% UCL 13.1 29.6 28.6 27.9 26.7 24.9 9.29 Mln 0 21 19 21 17 10 0 Max 22 39 32 32 33 33 18 StdErr 1.57 1.21 0.693 0.603 0.812 1.28 0.957 StdDev 8.61 6.64 3.79 3.3 4.45 7 5.24 cv% 87.0% 24.5% 14.0% 12.4% 17.8% 31.3% 71.5% Diff% 0.0% -174.0% -175.0% -170.0% -153.0% -126.0% 25.9% 000-089-163-3 CETIS™ v1.6.3revE Analyst:, CETIS Summary Report Report Date: Link/Link Coda: 04Apr-0816:31 (p2of 2) 20-8424-2555/0802-S056a Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.6 25 50 100 Control Type Conductivity Co Lab Control Rep1 0 1 1 1 1 Rep 2 1 1 1 1 1 1 1 Rep 3 1 1 1 1 1 1 1 Rep 4 1 1 1 1 1 1 1 Rep 5 1 1 1 1 1 1 1 Rep 6 1 1 1 1 1 1 1 Rep 7 0 1 1 1 1 1 1 Rep 8 0 1 1 1 1 1 1 Rep 9 1 1 1 1 1 1 1 Rep 10 1 1 1 1 1 1 1 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 0 23 26 30 24 Rep 2 11 26 24 28 27 10 0 Rep 3 4 23 29 28 23 26 7 Rep 4 11 22 27 28 30 33 18 Rep5 12 39 30 32 23 20 6 Rep 6 20 36 28 24 27 16 7 Rep 7 0 22 19 21 23 24 4 Rep 8 0 34 31 26 23 26 3 Rep 9 19 25 32 27 33 28 10 Rep 10 22 21 26 23 17 18 11 000-089-163-3 CETIS™ v1.6.3revE Analyst CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:19(pi of 1) 20-8424-2555/0802-S056a Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 17-3527-6295 Endpoint: 02 Apr-08 11:18 Analysis: 7d Survival Rate CETIS Version: CETIS v1. 6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% TestStat P-Value Decision(O.OS) Lab Control 6.25 1 12.5 1 25 1 50 1 100 1 Test Acceptability Attribute TestStat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- & 0.8- * 0.7- •| 0.6- W 0.5- S o.. 0.3- 0.2: 0.1- 0.0 Lab Control 10 0 10 0 10 0 10 0 9 0 9 0 • e • • 0 6.2S 12.5 25 Conc-% 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 9 9 • • 50 100 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:19(p1of 1) 20-8424-2555/0802-S056a Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 13-6665-8603 Analyzed: 02 Apr-08 11:18 Endpoinfc Reproduction Analysis: Parametric-Multiple Comparison Data Transform Zeta Alt Hyp Monte Carlo NOEL Untransformed Bonferronl Adj t Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100* Test Acceptability Attribute Control Resp PMSD OT Not Run 50 TestStat Critical MSD P-Value -0.0429 2.4 0.171 2.4 0.9 2.4 1.99 2.4 8.25 2.4 5.6 1.0000 5.6 1.0000 5.6 0.9300 5.75 0.1300 5.75 0.0000 TestStat Acceptability Limits Overlap 27.1 15 - NL 0.212 0.13-0.47 ANOVA Table Source Sum Squares Mean Square DF Between 2733.555 Error 1414.6 Total 4148.155 ANOVA Assumptions Attribute Test 546.7111 5 27.20385 52 573.9149 57 Yes Yes F Stat P-Value 20.1 0.0000 TestStat Critical P-Value Variances Barttett Equality of Variance 7.35 Distribution Shapiro-Wilk Normality 0.987 15.1 0.1960 0.7990 Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Min 0 Lab Control 10 6.25 10 12.5 10 25 10 50 9 100 9 Graphics 40-1 8 30~ 0 "*~ "* -4 g a >ft- 10- 27.1 24.6 27.2 25.8 26.7 25.4 25 23.3 22.3 19.7 7.33 5.34 t~ 0 Reject" •4 29.6 21 28.6 19 28 21 26.7 17 25 10 9.33 0 is- 10- M » 1 o -5- M -10- Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes LOEL TOEL TU PMSD 100 70.7 2 21.2% Decision(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Decision(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 39 1.23 6.64 24.5% 0.0% 32 0.705 3.79 14.0% -0.37% 32 0.613 3.3 12.4% 1.48% 33 0.826 4.45 17.8% 7.75% 33 1.3 7 31.3% 17.6% 18 0.974 5.24 71.5% 72.9% • */ ^ 0 6.25 12.S 25 50 100 -2.5 -Z.O -1.5 -1.0 -0.5 0.0 OS 1.0 U 2.0 2.5 Conc-% Rankits 000-089-163-3 CETIS™ v1.6.3revE AnalystrJ QA: CETIS Analytical Report Report Date: Link/Link Code: 02 Apr-OS 11 :19 (p 1 of 1 ) 20-8424-2555/0802-S056a Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 10-2843-5091 Endpoint: Reproduction Analyzed: 02 Apr-08 11:18 Analysis: Linear Interpolation (ICPIN) CETIS Version: Official Results: CETISvl .6.3 Yes Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 95% CL Method Linear Linear 7055475 280 Yes Two-Point Interpolation Test Acceptability Attribute Test Stat Acceptability Limits Overlap Decision Control Resp 27.1 15 -NL Yes Passes acceptability criteria Point Estimates Effect-% Conc-%95%LCL 95%UCL 50 79.2 69.5 88.4 Reproduction Summary Conc-% Control Type Calculated Variate Count Mean Min Max Std Err Std Oev CV%Oiff% 0 6.25 12.5 25 50 100 Lab Control 10 10 10 10 9 9 27.1 27.2 26.7 25 22.3 7.33 21 19 21 17 10 0 39 32 32 33 33 18 1.21 0.693 0.603 0.812 1.28 0.957 6.64 3.79 3.3 4.45 7 5.24 24.5% 14.0% 12.4% 17.8% 31.3% 71.5% 0.0% -0.37% 1.48% 7.75% 17.6% 72.9% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 RepS Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control 23 26 30 24 10 0 26 24 28 27 26 7 23 29 28 23 33 18 22 27 28 30 20 6 39 30 32 23 16 7 36 28 24 27 24 4 22 19 21 23 26 3 34 31 26 23 28 10 25 32 27 33 18 11 21 26 23 17 Graphics 000-089-163-3 CETIS™ v1.6.3revE QA: CETIS Analytical Report VS. Report Date: Link/Link Code: 04 Apr-08 16:31 (p 1 of 1) 20-8424-2555/0802-30563 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 14-1475-7807 Endpoint Reproduction Analyzed: 04 Apr-08 16:27 Analysis: Parametric-Two Sample Data Transform Untransformed Zeta Alt Hyp Monte Carlo NOEL C> T Not Run <0 Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Lab Control Conductivity Control 5 1.73 5.96 0.0000 ANOVA Table Source Sum Squares Mean Square DP FStat P-Value Between 1479.2 Error 1063.8 Total 2543 ANOVA Assumptions Attribute Test 1479.2 1 25 0.0001 59.1 18 1538.3 19 TestStat Critical P-Value Variances Variance Ratio F 1.68 6.54 0.4510 Distribution Shapiro-Wilk Normality 0.912 0.0685 Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Min 0 Lab Control 10 27.1 24.6 29.6 21 0 Conductivity Co 10 9.9 6.63 13.2 0 Reproduction Detail Conc-% Control Type 0 Conductivity C 0 Lab Control Graphics 40- 1 20- 1 0 Repf Rep 2 Rep 3 Rep 4 Rep 5 22 20 19 12 11 39 36 34 26 25 IS-i 1 "" !i •ga 0 -5- RigectNult Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 22.0% Decislon(5%) Significant Effect Declsion(5%) Significant Effect Decision{1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 39 1.23 6.64 24.5% 0.0% 22 1.6 8.61 87.0% 63.5% Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 11 4 0 00 23 23 22 22 21 / /*" 0 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 "1 Conc-% l^ffO Ranktti 000-089-163-4 CETIS™ v1.6.3revE Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Client/S, Test Nui ample ID fnber: Cone. LC Anal; Verlf : Dudek Carlsbad/Up*^^^a,\ jcfitoSL -Sc&bck Start Date/Time: 2/12/2008 .&•£? End Date/Time: 2/19/2008 O^^fi Rep 1 2 3 4s- 6 • 7 8 9 10 fSt edby: Board/ Cup ~i « °\ Q*l 1 1 Jt Hi" M"J -j M 3/7 \$&ff\ flw\, %fr Daily Reproduction/ Survival :i:-:j:fl£:!:i O oo o OO D 0 Oo £/A- :*:•:•: V; •:•:•o? o o c)oo O 0 Op • :•;•:-!*,•:•>'. ©o Q)o O O OV«J ;|;Hi;S;;;:-ji Ht c» c> O 0 f?Oo *?Gr :|:|:!Si:;:i:j O d dc) \i 1S ^t\ Ji 7j^g, •:[Hi|rii:|:|: 11*i»*17-IT-.5 it* *f!(x H 'V-ilfr ;;;j;ir8:;;;:;i * Total ^>n£* It/El?/l/l' ~^*t\3G> **"! QC <jj£r t 1- Cone. CC Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Rep 1 2 3 4 S 6 7 8 9 10 Daily Reproduction/ Survival ... .1 £8o (9 -O e? (? £3 <? </ *t/ • 0 j:!jN£j:;ji: O O 0 D d O ftec 0 :&:::3:::£ 0 fio(1) O O o/A Q/& O O |:j:;:|i|::i|i! O •2_ 2L CD_ - 1 2. ;:!;;;:5;;|;|i «9/^ O Oo o O_ - 0 o ij:i|::jB^:i|!;i ^. 51 C <-{ s> !o_ - k, 'Z :;;;i!i?fri:;H- t» H5 Il9 '9 — - f •:!!;iifti:iii: •*" Total O /<1 it Hn11- 1 0/4.O/JLITUJ^T^ N3o>- - Daily Reproduction/ Survival 1 ;;;:;;0 O O C?O 0 0 0 Oo ;;;:;:i2;:i;i; o o 6 o o o 0 0o 0 j:;|:i:3i:ii:|i O g) 0 Q> 0 O) n (3 Q O :j;:ij:8:!;!:i *f 2- *f f s 3 S <-( <- M j:ji|;i5j:j:j: V^ i~\~ O It 7- O 11 1. t!> •lij-j-fi:::::!1 •3- <? C> O Q> O {2 •«• !i|:::|:7i:!ij;; If i 'Sjliii ^ifS O ^4-|U j-ilj-iSlHi:: jv7 Total 2£* *^*t i2-1 JLT ao ii-V ^*1r3V <52. 2^? QC •-I < QC t<J 12.5% Rfin i 2 3 4 5 6 7 8 9 10 1 -;&i 0 <7 O O & 0 0o 9,0 i!i|l;i?i!l!=lt o o O6e *— , e>o O Daily F ^i!!l3!i:i!i ft n 00 0 0 OOnPJ ieprodu i^i;i»i:!ii;.^1s~&f& H H.r ction/S !;i;;ii5i!ii:!; O 0 0^ Q " $ f] 'J' 0*1 urvivalmmx\ \01n 7 - i£<; 7 9- lo c> ii:;:;7!;ij;;: l&" 'P£lb '& 0 (0 WV?w ;:*:$:;:;:;Total 2£? "^§ ^^"0z-v f?-JiT •2 1 2*Lf JPIA QC II fefr me Fed/Test Solution Renewed (day): . (11 t(fo (2)l^Sl_ (3)_JJULL .(5)JillI_(6) INS (7)..(8)_ d = dead; M = male; LIP = tost in progress; B = 4th brood (only the first 3 broods are included in total) >mments: 1C Check:Final Review: autilus Environmental 5550 Morehouse Drive. Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ifr Test Number: Dudek Carlsbad/Upotream Cffo3-.Sog6?di Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: End Date/Time: 2/12/2008 2/1 9/2008 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup ^| V 4 ^M f r A4\o M-2> 1(1 31sa I5"?aH *O-€.6- Daily Reproduction/ Survival ...f. O O O O 0 O O O O O JJA- 2 j D <} Cs 0 0 3 0 0 r*.V 0 Nrf 3 O O 0 0 0 0 O 0 ft O €6 4. 0 2- 2- H ^fyH L, H 3?VA/" *3#^> a£> 0I O O H ffr e ^10s11 6 (2,s 7 16 e> &, 1 I2)15 »£ IB IH-iih?\5-l>«? KiJ^ 8 Total 3Han ^?) ^0A34.1.asJ3 ^1t-i QC If t Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 • Daily Reproduction/ Survival .. IjlHii @> o 0 ^0 O <© 0 Or> w&m— Q 0 0 0 o 0 0 !!!!!!$)=!!!!.^_*><- O ft o 0 0 0 o 0 8 :i;j;;j4i;;j;ii . — & O *, 3 j§ M 3 2 & ;;!:!;$!!:!!;! — -. & 1 & o o t> 0 d •?, ;!!i|i;8jjji;;i ^_ <; <\ '3 S (, 9 <? '*^ |l!i;!!JJi!;!i;; ._ Dlo \S 12- ^h 12- \^i1*- |l llHHiSiiH!: — Total -. to o^/ fe«9-^; c^O \(j ^2O i^fl« QC 10 ' Cone. 100% Rep 1 I 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 'tiljlli ^?> 0 O p £> O O 6 0 O £iii:!%ii!i!*tr O 0 0 Oo C C O 0 ijiiiij&jpjl; **^6- O 0 0 a 0 (5 a0o Hi!*!:!!!! —0 0 H 3> O o o oo mmmi-* 0 I 5 0 •b U- 3o ,3 iiliiilSlli-iJ — ' <D <5 f 0 ^o 6 3 2, l|!!l:!?ji;i;( - ft l» J? 2. n C?0 9- (a .;i;i;i$;:i!;; - Total —o •J 1^u 7 if 3 10 H QC O ? Time Fed/Test Solution Renewed (day): Notes: d = dead; M = mate; UP = lost In progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Check: Iff-Final Review: Nautilus Environmental 5550 Monhouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client: Dticfok Carlsbad Sample ID: "UpotreamV*— Test No: Concentration Day oH DO (mg/L) Cond. (pmhos/cm) Tem 'C) PH DO (mgA.) Temp (*C) Concentration Day PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) PH DO (mg/L) Temp {'C) Concentration Day PH DO (mg/L) Cond. ((imhos/cm) Tem PC) Water Quality Measurements Test Species: C. dub/a Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 Lab Control Q... I J . L..« I. 3. an * ZH.7 71 6^^ a, 10 f J . 0 -7 6.25% nU^Ul.,,.! ..3. 5? "7-7 AS .-7 12.5% 4«fr PH DO (mg/L) Temp (°C)ZM.7 7. -Z.M.5 •7-0 -11 V.1.0 LS-f 33. as:? Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: Sample Log-in Numbers: QC Check: Yf es A A A of A to- A /v pp A:B= __ Animal Age at Inittatjon: <- gKf\ N|*T C: Nautilus Environmental 5550 More/rouse DrrVe, Surte J50. San D/ego, CA 92127. Freshwater Chronic Bioassay Water Quality Measurements Client: _ Dudek Carlsbad Sample ID: U P Test No: Test Species: C. cfa/fr/a Start Date/Time: 2/12/2008 I End Date/Time: 2/19/2008 Concentration Da 25% PH DO (mg/L) Cond. (Mmhos/cm) Temp ('C> UU'b PH DO (mg/L) Temp (°C)as.i -to-o Concentration Da 50% PH DO (mg/L) Cond. (pmhos/cm) Temp °C) •&.S 103 1> 2.M.S PH DO (mg/L) Temp (°C) -7^0 Concentration Day 100% PH DO (mg/L) Cond. (pmhos/cmjf v Temp (°C)£ -1-2. 1H. . *« ^-71*7 PH DO (mg/L) Temp(°C)ZM.7 £8.}-2-5:3 Analysts: Initial: Final: Sample Used (A, B, C):A bF /v A A A Comments! Animal Source/Date Received: Sample Log-in Numbers: A: QC Check: fcf as.7 €.5 515 -" S.SS B: Animal Age at Initiation: C: Final Review: /yfo Nautilus Environmental 5550 More/loose OoVe, Sufte f 50. San O/ego, C-4 92 Hf . Site: Surfaces CETIS Summary Report Report Date: Link/Link Code: 02Apr-0811:40(p1of 2) 17-8656-1068/0802-S056b Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 16-8344-7753 Test Type: Reproduction-Survival (7d) Start Date: 12 Feb-08 16:00 Protocol: EPA/82 1/R-02-01 3 (2002) Ending Date: 19Feb-08 09:50 Species: Ceriodaphnia dubia Duration: 6d 18h Source: In-House Culture Sample No: 04-1838-3807 Code: 08-0167 Sample Date: 11 Feb-08 12:15 Material: Downstream Ambient Sample Receive Date: 12 Feb-08 14:30 Source: Dudek & Associates Sample Age: 28h(5.5*C) Station: Surface 3 Comparison Summary Analysis No Endpoint 06-8918-0716 7d Survival Rate 08-7618-4808 Reproduction Point Estimate Summary Analysis No Endpoint 10-1584-0586 Reproduction Test Acceptability Analysis No Endpoint 06-8918-0716 7d Survival Rate 08-7618-4808 Reproduction 10-1584-0586 Reproduction 08-7618-4808 Reproduction NOEL LOEL 100 > 100 50 100 Effect-% 50 Attribute Control Resp Control Resp Control Resp PMSD Conc-% 86.1 TestStat 0.9 22.6 22.6 0.279 TOEL N/A 70.7 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Age: <8h Client: Dudek & Associates Project: PMSD N/A 27.9% Method Fisher Exact/Bonferroni-Holm Test Wilcoxon/Bonferroni Adj Test 95% LCL 95% UCL Method 77.8 95.3 Linear Interpolation (ICPIN) Acceptabilty Limits Overlap 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 9 10 Mean 0.7 0.9 0.9 1 1 1 0.7 95% LCL 0.52 0.782 0.782 1 1 1 0.52 95% UCL 0.88 1 1 1 1 1 0.88 Mln 0 0 0 1 1 1 0 Max 1 1 StdErr 0.0882 0.0577 0.0577 0 0 0 0.0882 StdDev 0.483 0.316 0.316 0 0 0 0.483 CV% 69.0% 35.1% 35.1% 0.0% 0.0% 0.0% 69.0% Difr% 0.0% -28.6% -28.6% -42.9% -42.9% -42.9% 0.0% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 9 10 Mean 7.6 22.6 22.6 26.3 24.8 23 7.8 95% LCL 5.96 20 19.3 24.6 23.2 21.9 5.65 95% UCL 9.24 25.2 25.9 28 26.4 24.1 9.95 Mln 0 5 0 20 19 19 0 Max 14 30 30 35 33 28 18 StdErr 0.804 1.26 1.59 0.821 0.783 0.524 1.05 StdDev 4.4 6.92 8.73 4.5 4.29 2.87 5.77 cv% 57.9% 30.6% 38.6% 17.1% 17.3% 12.5% 74.0% Diff% 0.0% -197.0% -197.0% -246.0% -226.0% -203.0% -2.63% 000-089-163-3 CETIS™ v1.6.3revE Analyst:, CETIS Summary Report Report Date: Link/Link Code: 02Apf-0811:40(p2of 2) 17-8656-1068/0802-S056b Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 Rep 2 0 1 0 1 1 1 1 Rep 3 0 1 1 1 1 1 1 Rep 4 1 0 1 1 1 1 1 Rep 5 1 1 1 1 1 1 0 Rep 6 1 1 1 1 1 1 1 Rep 7 0 1 1 1 1 1 0 Rep 8 1 1 1 1 1 1 1 Rep 9 1 1 1 1 1 1 0 Rep 10 1 1 1 1 1 1 1 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 10 23 20 28 24 11 Rep 2 4 25 0 30 28 24 12 Rep 3 3 21 29 20 33 24 18 Rep 4 14 5 26 25 19 23 6 Rep 5 12 26 30 23 24 22 4 Rep 6 9 28 20 35 23 20 11 Rep 7 0 26 28 26 29 21 5 Rep 8 10 21 26 24 20 28 0 Rep 9 5 30 26 30 22 19 0 Rep 10 9 21 21 22 26 26 11 000-089-163-3 CETIS™ v1.6.3revE CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:39(p1of 2) 17-8656-1068AD802-S056b Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 08-7618-4808 02 Apr-08 11:39 Data Transform Rank Zeta Wllcoxon/Bonferroni Adj Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100* Test Acceptability Attribute Control Resp PMSD ANOVA Table Source Between Error Total Endpoinfc Reproduction Analysis: Nonparametric-MuKipte Comparison Alt Hyp OT Test Stat 108 120 110 83.5 61.5 Monte Carlo Not Run Critical Test Stat Acceptability Ties 4 5 3 4 1 Limits 22.6 15 -NL 0.279 0.13-0.47 Sum Squares 2253.527 1830.1 4083.627 Mean Square 450.7054 34.53019 485.2356 ANOVA Assumptions Attribute Test Variances Distribution Bartlett Equality of Variance Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type 0 6.25 12.5 25 50 too Lab Control Rank Transformed Summary Conc-% Control Type 0 6.25 12.5 25 50 100 Lab Control Count Mean 10 10 10 10 9 10 Count 10 10 10 10 9 10 22.6 22.6 26.3 24.8 23 7.8 Mean 32.8 34.3 40.6 35.6 29.7 7.05 DF 5 53 58 Test Stat 11.7 0.895 95% LCL 20 19.3 24.6 23.2 21.9 5.61 95% LCL 27 27.3 35.2 30 25.3 5.69 FStat 13.1 Critical 15.1 95% UCL 26.2 25.9 28 26.4 24.1 9.99 95% UCL 38.6 41.2 45.9 41.2 34.1 8.41 NOEL 50 P-Value 1.0000 1.0000 1.0000 1.0000 0.0007 Overlap Yes Yes P-Value 0.0000 P-Value 0.0394 0.0001 Mln 5 0 20 19 19 0 Min 5.5 2 17 13.5 13.5 2 CETIS Version: Official Results LOEL TOEL 100 70.7 Decision(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision Nautilus Environmental (CA) CETISvl.6.3 Yes TU PMSD 2 27.9% Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Decision(1%) Equal Variances Non-normal Distribution Max Std Err 30 1.28 30 1.62 35 0.835 33 0.797 28 0.533 18 1.07 Max Std Err 55.5 2.83 55.5 3.39 59 2.63 58 2.76 49 2.15 12 0.662 Std Dev CV% 6.92 30.6% 8.73 38.6% 4.5 17.1% 4.29 17.3% 2.87 12.5% 5.77 74.0% Std Dev CV% 15.3 46.5% 18.2 53.2% 14.2 34.9% 14.8 41.7% 11.6 38.9% 3.56 50.5% Diff% 0.0% 0.0% -16.4% -9.73% -1.77% 65.5% Diff% 0.0% -4.42% -23.6% -8.54% 9.38% 78.5% 000-089-163-3 CETIS™ v1.6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:39(p2of 2) 17-8656-1068/0802-S056b Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 08-7618-4808 Analyzed: 02 Apr-OS 11:39 Endpoinfc Reproduction Analysis: Nonparametric-MutBpte Comparison CETIS Version: CETISv1.6.3 Official Results: Yes Graphics 30- 20- 10- 30- 2Q-. 10- 0 -10- -2th 6.25 12.5 25 Conc-% -40 50 100 -2.S -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1J 2.0 Z.S Rankits 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-Q811:40(p1 of 1) 17-8656-1068/0802-S056b Cerfodaphnla 7-d Survival and Reproduction Test Analysis No: 10-1584-0586 Endpolnt Reproduction Analyzed: 02 Apr-08 11:39 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Resamples Linear Linear 5334240 280 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes Exp 95% CL Method Yes Test Acceptability Attribute TestStat Acceptability Limits Control Resp 22.6 15 -NL Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95%LCL 95% UCL 50 86.1 77.8 95.3 Reproduction Summary Conc-% 0 6.25 12.5 25 50 100 Control Type Count Lab Control 10 10 10 10 9 10 Mean 22.6 22.6 26.3 24.8 23 7.8 Mln 5 0 20 19 19 0 Calculated Variate Max 30 30 35 33 28 18 Std Err 1.26 1.59 0.821 0.783 0.524 1.05 StdDev 6.92 8 73 4.5 4 2 5 29 87 77 CV% 30.6% 38.6% 17.1% 17.3% 12.5% 74.0% Dlff% 0.0% 0.0% -16.4% -9.73% -1.77% 65.5% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 30- 25- < g 20- 5- Control Type Rep 1 Lab Control 23 20 28 24 24 11 f"-*.~ 0 20 40 60 Conc-% Rep 2 25 0 30 28 24 12 \ \ 80 Rep 3 21 29 20 33 23 18 \ 100 120 Rep 4 5 26 25 19 22 6 Rep 5 26 30 23 24 20 4 Rep 6 28 20 35 23 21 11 Rep 7 26 28 26 29 28 5 Rep 8 Rep 9 21 30 26 26 24 30 20 22 19 26 0 0 Rep 10 21 21 22 26 11 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 02Apr-0811:40(p1of 1) 17-8656-1068/0802-80566 Cerfodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 06-8918-0716 Endpoinfc 02 Apr-08 11 :39 Analysis: 7d Survival Rate CETIS Version: CETISvl .6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferronl-Holm Test Control vs Conc-% TestStat P-Value Decision(O.OS) Lab Control 6.25 0.763 12.5 1 25 1 50 1 100 0.291 Test Acceptability Attribute TestStat Control Resp 0.9 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- £ 0*a 0.7- 1 H 5 0.5- S o> 0.3- 0.2- 0.1- 0.0 Lab Control 9 1 9 1 10 0 10 0 9 0 7 3 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 9 10 • • • • • • 0 6.2S 12,5 25 Conc-% SO 100 000-089-163-3 CETIS™ v1.6.3revE Analyst CETIS Analytical Report /. (i«t t£f ReP°rtDate: Link/Link Code: o2Apr-o8H:40<piof ij 1 7-8656-1 068/0802-S056b Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: Nautilus Environmental (CA) 09-0530-2777 Endpoinfc Reproduction CETIS Version: CETISv1.6.3 02 Apr-OS 11:40 Analysis: Parametric-Two Sample Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run Equal Variance t Two-Sample Test Control vs Control TestStat Critical Lab Control Conductivity Control 5.79 1.73 <0 0 N/A 19.9% MSD P-Value Decision(5%) 4.5 0.0000 Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp Control Resp PMSD 22.6 15 -NL 7.6 15 -NL 0.199 0.13-0.47 ANOVA Table Source Sum Squares Mean Square DF Between Error Total 1125 1125 1 604.8 33.6 18 1729.8 1158.6 19 Yes Passes acceptability criteria Yes Fails acceptability criteria Yes Passes acceptability criteria F Stat P-Value Decision{5%) 33.5 0.0000 Significant Effect ANOVA Assumptions Attribute Test TestStat Critical P-Value Decision(1%) Variances Distribution Variance Ratio F 2.47 Shapiro-Wilk Normality 0.876 6.54 0.1950 Equal Variances 0.0150 Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Min Max StdErr StdDev CV% Diff% 0 0 Graphics 40- § 30- a 10- Lab Control 10 22.6 20 Conductivity Co 10 7.6 5.93 Reject* 0 0 Q(XV'**oW"*^f conc-% Jj?vB 25.2 5 30 1.28 6.92 30.6% 0.0% 9.27 0 14 0.817 4.4 57.9% 66.4% l<h ^~ ^s • a .S^ -10- • /^ uii * -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 RanKrts 000-089-163-3 CETIS™ v1.6.3revE QA: Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Client/Sample Test Number: Dudek Carlsbad/Downstream Start Date/Time: End Date/Time: 2/12/2008 2/19/2008 Cone. LC Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ CUD f *tt °\< c C 10JO. #1 oL \o437 ,T7fj#. T/ SMstf OF <r6 Daily Reproduction/ Survival ilhn&iHiii (9o 0 0 ,* 0 « 0• <oo 0o/>w~ :*£?** 0 Pc; O O._\ C CA 0 A/tf !ji&!3;:!£ fe v»> 0ftaft oooo &<, mv*w\ 5 £ M *>/«/ -5 f> &• JT & •*> c>w #;:JSi#i:i 0 O F < 0 0 0 010m i:;:!iS$H!; & 9: "S^r7_ fe ^ ^S !-i % &^ wmm i~L JJ ^is 14- '.rii 12, f«wo- !iii!!?ii!iii! — Total Xb •Q«6 ^)i£/sT oO.a.^^.c^ ^J2&^l QC U Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 :j:!Hf) ft 0 (0 On Do 00 i;i;i:!Z!:!i;; D Q 0 0 0 0o 0 6 O m$mo 6 e 6 0 6 » 0 0 0 mmm CO <J> C3 ^3 4 °/<3l <s> (9 a i!lj;!i5!i;!!i- 1 'A /)VL/3_ 5i3 is;;;si*h: 32/ril3/-A 6 0 5 — ~2- 3 L ;-:;i::7::-;::i t" — S £o— J? ;:=ii;«:ii!:l — — — Total |U 4n/A A/A 't1^ ^ jofd. 10^<*f Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 #!:! O #°0nc> o fl& 0& =!H!!J2!£H O —o f> 0 oo 0 Oo mmmo• — o r> o & O O<?<? mmx 3> 4-^- s~ LI If £• ^r -7 C, *i !!:!;!;ai;;i;! f) _—-_. 0 o 6 D0 0 0<i mmms —T 10 12 tr ^~? 7 ~? j:!:::!?:-;-::; 12-_-. \S 13- )i- *f- •4- ,(?'A3 Irt I!:!:!®:!:;:! — Total *Hof A. A*\ cOCO 5O Al Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 liliji 0e> 0 h 0 0Qn 0 0 )i!*!Z!;!;ii •y 6 0 0 6 0 3 Ci 0 p ;ili;!;?;:!r;i /*> n <o «»» £5 t> Oodc> !iH!:i*!!!l! H 3 Hjs- JB~ (^. ^H2 2_ !!!!!!j§N#i ^0 0oti o 0 0 0f mmm& \0<{ ^-7 O 7 <*i5 f2_ ;;i=:;7;;i!;:;u ^-^ 17. Mt(rV*\7^ ?^ T- &8KS&Total 25?52? >2D ^^4.3 35 2(fi. ?Aac>0^- QC ~7 QC \B QC 15 me Fed/Test Solution Renewed (day): lotes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) amments: 1C Check:Final Review lautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Client/Sample ID: Pudek Carlsbad/Pownstfeamgi Test Number: Qfrc&.-<SQgG?b start Date/Time: End Date/Time: 2/12/2008 2/19/2008 Cone.Rep Board/ Cup Daily Reproduction/ Survivale Total QC 25%1 0 iSL 0 0 «***/: 0 0 #7 0 n 0 f) 37 & 10 0 0 10 10 If? Analyst DP Verified by: ML Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survivalmm6 6 0 0 *g> (D 0 C) 8> f) !!!i!!2!!!i;t i-»flCO 0 n o p o •0 0 Q I!!!!!*!!)!!! 6 0 0 0 0 0 & n G<? !;IHliJN!;!!il - jr 1 3 1 ^,? JT f -? liilHSilliil —o 5 0 / 6 0 \o o mmm- <«-? In 7 n 6 3 ;W£** .- Mt<? 10 1toit \i ^1p ;!!!!!!B!£!! -• Total 0/W^ c^Jf **i*-3 JJ3- c^.D "^oio 11 A^ QC I -2-£6- Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival ' . iilHiHl!! <D 0 0 o to 0 6 Oo 0 0& 0 o C 0 0 0 G 0 2. H j. O - 0 — —0 H!!!!!£J!!!! Iff 5 lor>. — #- o —"r i!i=!i!il!iii!l — — — Total u 1%I** ^nt/<£ h ^4.,oM1 e7/d\\-Mc QC 0 Time Fed/Test Solution Renewed (day):IH'i.T . . Notes: d = dead; M = male; LIP = lost In progress; B = 4th brood (only the first 3 broods are included in total) Comments: >, AJ 0 QC Check:Final Revlew Nautilus Environmental 5550 More/rouse Drive, Suite 150. San Diego. CA 92121. Freshwater Chronic Bioassay Client: Dudek Carlsbad Water Quality Measurements Test Species: C. dubia _ Sample ID: • Downstream •-£', Test No: Start Date/Time: 2/12/2008 lacrO End Date/Time: 2/19/2008 Concentration Day Lab Control o 1 . 2 I 3 pH DO (mg/U Cond. (pmhos/cm) Temp CO 71s 7. H 2-6.3 pH DO (mg/L) Temp (°C)2H7 S IP? IS-'* f.05 -S Concentration Da Conductivity Control PH DO (mg/L) Cond. (|rnihos/cm) Temp ( ' •.£'>*& "C) T.I PH DO (mg/L) Temp (°C) 7,7 33^ -1M.Z 3£L ^. '. .PI •7.* 2-5*5 -as.7 Concentration Da PH DO (mg/L) Cond. (nmhos/cm) Temp "C) 6.25% 1 1 .2 «... «.. -?-X-IH 7-7 2.H.1 , 2. MSR PH DO (mg/L) Temp (°C). J 2-fT. S Concentration Day 12.5% PH DO (mg/L) Cond. ((imhos/cm) Temp CJ K.O3 ?-OM •7-7 PH DO (mg/L) Temp (°C)Z-M.7 : .rigal 6 , 1, , 7 •7-t -. n 8.3 Analysts: Initial: Final: Sample Used (A, B, C): Comments: 3 Animal Source/Date Received: Sample Log-in Numbers: QCChecK: A A Of A MP A B: tlj/V Animal Age at Initiation: C: N Final Review: A Nautilus Environmental 5550 More/loose Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client: _ Dudek Carlsbad Sample \D: Test No: Test Species: C. dubfa Start Date/Time: 2/12/2008 End Date/Time: 2/19/2008 Concentration Da 25% PH DO (mg/L) Cond. (pmnos/cm) Tempra V PH DO (mg/L) Temp CO Concentration Da PH DO (mg/L) Cond. (pmhos/cm) Temp (*C) PH DO (mq/L) Temp (*C) Concentration Day PH DO (mg/L) Cond. (pmhos/cm) Temp (°C) PH DO (mart.) Temp (*C) Analysts: Initial: Final: Sample Used (A, B, C): ZH.7 35, i H -2-6.0 .1* :.y. n.o 50% 7.16 1.0 <\D' 8.38 as.) 100% IS .3 JLS_ 2.M.7 A £5 OF T T56 Of A Comments: Animal Source/Date Received: Sample Log-in Numbers: A: QC Check: far ft as. •? 33OO ,\ -2.5.0 as r? JSE. Animal Age at Initiation: C: Final Review: Nautilus environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Appendix B Sample Receipt Information Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Client: Sample ID: Tests Performed: TestlDNo(s).: Sample (A, B,C): Log-in No. (08-xxxx): Sample Collection Date & Time: Sample Receipt Date & Time: Check-in Temperature (°C) Temperature OK? DO (mg/L) pH (units) Conductivity (pS/cm) Salinity (ppt) Alkalinity (mgfl.)* Hardness (mg/L)*' Total Chlorine (mg/L) Technician Initials SurfcCA. 3 i£S*< 130 u.o 10.5 5.5 10.0 1.97 33-70 l.Q? 0.02, ©N VI IM 3 N £><• OS 0.61 &S- • • mg/L as CaCOj, * • Measured for freshwater samples only, MA=Not Applicable /Freshwater Tests; 1 Control/Dilution Water Source: (&> Culligan Other Additional Control? Y N = Marine Testa: Control/Dilution Water Source: LABSW ARTSW Other. Additional Control? Y N = Alkalinity:. .Alkalinity:. Alkalinity:. Alkalinity:. Hardness:. Hardness:. Salinity:. Salinity:. Sample Salted w/ artificial salt? Y N If yes, what ppt?. Sample salted w/brine? Y N If yes, what ppt?. Sample Check-In Information Sample Description: odor Us j - "v\ Initial Freed,: STS added; Final Free CI2: Sub-samples for additional chemistry: Comments: Temperature for sample must be 0-6°C if received >24 hours past collection time. QC Check: Final Review: AC Appendix C Chain-of-Custody Form Vi CALIFORNIA Q WASHINGTON O BRITISH COLUMBIA '^SO/taehouse&Ne-SutelSO 5009 PotfeHgliwoy EosN Suite 2 8664 Commerce Court Son Diego, Cofanio 92121 k^Vteti^ 98424 Burnoby, British Cokwihio, Conodo V5A 4N3 Phone 858.587.7333 Phone 253.922.4296 Phone 604.420.8773 Fox 858.587.3961 Fox 253.922.5814 Fox 604.357.1361 Chain of Custody A_ \MufiZ / ., / ^ J \\ f)Page of Sn.npfcCnll^nhy. SAMPLED ANALYSES REQUIRED DATE TIA^E MATRIX CONTAINER 1YT6 NUMKKOFCONTAMERS COMMENTS it 11 X PROJKT INFORMATION CUENT SAMPl£I$CHCT ro NO^. OF COKWNERS RECD GOOD CONDITION 4 REUNQUISHEO BY (CUENT)REUNQUISHED BY (COURIER) ISiana ITimel (Signotura) (Printed Nome)(Date) SHIPPED VIA:MATCHES TESISCHEDUIE SPEOAllNSIRUCnONS/COMMtNTS: (Company](Company) RECEIVED BY (COURIER) -•». -as: (Signature](Tinw) (Printed (Company)Nautilus fe (Time); Additional costs may be required for sample disposal or storage. Net 30 unless otherwise contracted.A/ T " Nautilus Environmental,ginator June 23, 2008 EnviroMatrix I M I Analytical, Inc. 23 June 2008 Dudek & Associates, Inc. EMA Log #: 0806245 Attn: Chris Oesch 605 Third Street Encinitas, CA 92024 Project Name: Carlsbad Golf Course Enclosed are the results of analyses for samples received by the laboratory on 06/12/08 13:11. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. I certify that this data isin compliance both technically and for completeness. \ Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewridge Avenue, Suite A • San Diego, California 92123 • (858) 560-7717 • Fax (858) 560-7763 A n.kr»: •Client Name: Dudek & Associates, Inc. EMALogft 0806245 Project Name: Carlsbad Golf Course ANALYTICAL REPORT FOR SAMPLES SampltlP Laboratory IP Matrii D«t« Sampled Downstream Surface 0806245-01 Water 06/11/0811:20 06/12/0813:11 Upstream Surface 0806245-02 Water 06/11/0811:50 06/12/0813:11 Well #3 0806245-03 Water 06/11/0812:30 06/12/0813:11 Well#l 0806245-04 Water 06/11/0812:45 06/12/0813:11 Tie results in this report apply to the samples analyzed in accordance with the chain of •tscody document. This analytical report must be reproduced in to entirety. Page 2 of 8 K ~ Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EM A Log #:0806245 Conventional Chemistry Parameters by Standard/EPA Methods Analyte Result Downstream Surface (0806245-01) Water Sampled titrate as N Nitrite as N Dissolved Oxygen >H tiosphorus, Total Total Suspended Solids jpstream Surface (0806245-02) Water titrate as N •litrite as N )issolved Oxygen iH 'hosphorus, Total Total Suspended Solids iVell #3 (0806245-03) Water Sampled: titrate as N Nitrite as N Dissolved Oxygen >H 'hosphorus, Total Total Suspended Solids 0.23 ND 6.04 7.81 0.13 ND Reporting Limit Units Dilution Batch Prepared Analyzed Method Notes : 06/11/08 11:20 Received: 06/12/08 13:11 0.05 0.05 0.10 0.10 0.05 20 Sampled: 06/11/08 11:50 0.32 ND 6.02 7.86 0.11 ND 06/11/08 12: 19.7 ND 3.38 6.65 0.39 299 Well #1 (0806245-04) Water Sampled: 06/11/08 12 titrate as N Nitrite as N Dissolved Oxygen >H Phosphorus, Total Total Suspended Solids 0.07 ND 2.46 5.94 0.21 99 0.05 0.05 0.10 0.10 0.05 20 30 Received: 1.25 0.05 0.10 0.10 0.05 20 45 Received: 0.05 0.05 0.10 0.10 0.05 20 mg/1 1.• ii " pH Units mg/1 *t II Received: 06712/08 mg/1 1 n n « pH Units " mg/1 11 II 06/12/08 13:11 mg/1 25 1 » pH Units " mg/1 " " " 06/12/08 13:11 mg/1 1 II H .. pH Units " mg/1 n n 8061708 8061205 8061823 8061301 8062311 8061616 13:11 8061708 8061205 8061823 8061301 8062311 8061616 8061708 8061205 8061823 8061301 8062311 8061616 8061708 8061205 8061823 8061301 8062311 8061616 06/17/08 06/13/08 06/1 1/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 06/17/08 06/13/08 06/11/08 06/12/08 06/23/08 06/16/08 SM4500 NO3 E SM4500 NO2 B SM4500-O G SM4500-H+ B SM4500 P B, E SM2540D SM4500 NO3 E SM4500 NO2 B SM4500-O G SM4500-H+ B SM4500 P B, E SM2540 D SM4500 NO3 E SM4500 NO2 B SM4500-OG SM4500-H+ B SM4500 P B, E SM2540 D SM4500 NO3 E SM4500NO2B SM4500-OG SM4500-H+ B SM4500 P B, E SM2540 D W-02 HT-15 HT-15 W-02 HT-15 HT-15 W-02 HT-15 HT-15 W-02 HT-15 HT-15 ie results in this report apply to the samples analyzed in accordance with the chain of istody document. This analytical report must be reproduced in its entirety. EnviroMatrix [ MA JAnalytical. Inc.Page 3 of 8 I Client Name: Dudek & Associates, Inc. I Project Name: Carlsbad Golf Course EMALogft 080(245 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control JAnalyte Reporting Result Limit Spike Units Level Source %REC Result %REC Limits RPD RPD Limit Notes Batch 8061205 Blank (8061205-BLK1)Prepared & Analyzed 06712/08 Nitrite as N *LCS (8061205-BS1) ND 0.05 mg/l Prepared & Analyzed: 06/12/08 Nitrite as N LCS Pup (8061205-8801) 0.10 0.05 mg/l 0.100 103 80-120 Prepared & Analyzed: 06/12/08 Nitrite as N Duplicate (806120S-DUP1) 0.10 0.05 mg/l 0.100 105 80-120 2 Source; 0806216-01 Prepared & Analyzed: 06/12/08 20 Nitrite as N Matrix Spike (806120S-MS1) 0.18 0.05 mg/l 0.19 Source: 0806216-01 Prepared & Analyzed 06/12/08 20 Nitrite as N Matrix Spike Pup (8061205-MSD1) 0.29 0.05 mg/l 0.100 0.19 102 80-120 Source; 0806216-01 Prepared & Analyzed: 06/12/08 Nitrite as N Batch 8061301 0.31 0.05 mg/l 0.100 0.19 120 80-120 20 Duplicate (8061301-DUP1)Source; 0806216-01 Prepared & Analyzed: 06/12/08 PH Reference (8061301-SRM1) 8.68 0.10 pH Units 8.67 Prepared & Analyzed: 06/12/08 0.2 20 HT-15 pH Batch 8061616 9.16 0.10 pH Units 9.10 101 7.03-102.9 Blank (8061616-BLK1)Prepared & Analyzed: 06/16/08 Total Suspended Solids ND 20 mg/l The results in this report apply to the samples analyzed in accordance with the chain of •vslody document. This analytical report must be reproduced in its entirety. oHati Page 4 of 8 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EMALog#: 0806245 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Reporting Spike Source %REC RPD Result Limit Units Level Result %REC Limits RPD Limit Notes Batch 8061616 Duplicate (8061616-DUP1)Source; 0806245-01 Prepared & Analyzed: 06/16/08 Total Suspended Solids Reference (8061616-SRM1) 10 20 mg/1 12 18 20 Prepared & Analyzed: 06/16/08 Total Suspended Solids Batch 8061708 Blank (8061708-BLK1) Nitrate as N LCS (8061708-BS1) Nitrate as N LCS Dup (8061708-BSD1) Nitrate as N Duplicate (8061708-DUP1) Nitrate as N Duplicate (8061708-DUP2) Nitrate as N Duplicate (8061708-DUP3) Nitrate as N Matrix Spike (8061708-MS1) Nitrate as N 42 ND 0.50 0.50 17.1 16.9 ND 0.45 20 0.05 0.05 mg/I mg/1 mg/1 0.05 mg/1 Source: 0805112-05 2.50 mg/1 Source: 0805112-05 2.50 mg/1 Source: 0806229-03 0.05 mg/1 Source: 0806229-03 0.05 mg/I 50.3 83 81.9-106.8 Prepared & Analyzed: 06/17/08 Prepared & Analyzed: 06717/08 0.500 101 80-120 Prepared & Analyzed: 06/17/08 0.500 100 80-120 0.6 Prepared & Analyzed: 06/17/08 17.3 1 Prepared & Analyzed: 06/17/08 17.3 3 Prepared & Analyzed: 06/17/08 ND Prepared & Analyzed: 06/17/08 0.500 ND 90 80-120 20 20 20 20 'Tie results in this report apply to the samples analyzed in accordance with the chain of lutody document. This analytical report must be reproduced in its entirety. A J Analytical, Inc. Page 5 of 8 | Client Name: Dudek & Associates, Inc. • Project Name: Carlsbad Golf Course Conventional Chemistry JAnalyte Result EMALog#: 0806245 Parameters by Standard/EPA Methods - Quality Control Reporting Spike Source %REC RPD Limit Units Level Result %REC Limits RPD Limit Notes Batch 8061798 Matrix Spike Dup (8861708-MSD1) Nitrate as N 0.50 Reference (8061708-SRM1) Nitrate as N 0.62 Batch 8061823 Duplicate (8061823-DUPl) Dissolved Oxygen 5.77 Duplicate (8061823-DUP2) Dissolved Oxygen 6.19 Duplicate (8061823-DUP3) Dissolved Oxygen 3.09 Duplicate (8061823-DUP4) Dissolved Oxygen 2.63 Batch 8062311 Blank (806231 1-BLK1) Phosphorus, Total ND LCS (8062311-BS1) Source: 0806229-03 Prepared & Analyzed: 06/1 7/08 0.05 mg/1 0.500 ND 100 80-120 11 20 Prepared & Analyzed: 06/17/08 0.05 mg/1 0.621 101 89.2-108.5 Source: 0806245-01 Prepared & Analyzed: 06/1 1/08 0.10 mg/1 6.04 5 20 Source: 0806245-02 Prepared & Analyzed: 06/1 1/08 0.10 mg/1 6.02 3 20 Source: 0806245-03 Prepared & Analyzed: 06/1 1/08 0.10 mg/1 3.38 9 20 Source: 0806245-04 Prepared & Analyzed: 0671 1/08 0.10 mg/l 2.46 7 20 Prepared & Analyzed: 06/23/08 0.05 mg/1 Prepared & Analyzed: 06/23/08 HT-15 HT-15 HT-15 HT-15 Phosphorus, Total 0.49 0.05 mg/1 0.500 98 80-120 The results in this report apply to the samples analyzed in accordance with the chain of tatody document. This analytical report mat be reproduced in its entirety. BnviroMatrix rCi. lAn-lyti.Page 6 of8 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EM A Log #: 0806245 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Spike Source %REC RPD Limit Units Level Result %REC Limits RPD Limit Notes Batch 8062311 LCSDup(8062311-BSDl)Prepared & Analyzed: 06/23/08 Phosphorus, Total Duplicate (8062311-PUP1) 0.49 0.05 mg/1 0.500 98 80-120 0.2 Source; 0806245-01 Prepared & Analyzed: 06/23/08 20 Phosphorus, Total Matrix Spike (8062311-MS1) 0.12 0.05 mg/l 0.13 4 Source: 0806245-01 Prepared & Analyzed: 06/23/08 20 Phosphorus, Total Matrix Spike Pup (8062311-MSD1) 0.61 0.05 mg/1 0.500 0.13 97 80-120 Source; 0806245-41 Prepared & Analyzed: 06/23/08 Phosphorus, Total 0.60 0.05 mg/1 0.500 0.13 95 80-120 20 he results in this report apply to the samples analyzed in accordance with the chain of atody document. This analytical report must be reproduced in its entirety. BnviroMatrix | MA ] Analytical, Inc.Page 7 of 8 Client Name: Dudek & Associates, Inc. EMALog#: 0806245 Project Name: Carlsbad Golf Course Notes and Definitions W-02 The sample for nitrate analysis was preserved with H2SO4 after the nitrite portion of the analysis was completed to extend the holding time for the sample. Nitrate results are corrected for the nitrite contribution per the method. HT-15 This sample was received outside of the EPA's recommended 15 minute holding time for this analysis. However, the sample was analyzed immediately upon receipt. ND Analyte NOT DETECTED at or above the reporting limit NR Not Reported dry Sample results reported on a dry weight basis RPD Relative Percent Difference The results In this report apply to the samples analyzed in accordance with the chain of Custody document This analytical report must be reproduced in its entirety. Page 8 of 8EmtroMatrix I Ml Analytical, lac. •• CHAIN-OF-CUSTODY FORM Analytical, Inc. AVfc,Sle. A • San MHO, CA 91123 • Phone (S5S) SW-7717 water WW-wastc water DW-drinking water SW-nom water A-iir SED-snOment O-oil T-Ussue S-tolid other (tpedty) L-Hquid odwr («pedry) Same day 24 hr -:48hr Return to dient: PAJ or Delivery r Archive uotU Properfy Preserved: Yes No N/A emporature @ Receipt:ustDdyStaUtoUictiVei No N/A OOLabeU Agree: Yd No N/A Pink- Client (w/Report)White-EMA Canary * Accounting OoMennxl - Qicnt (Relinquish Simple.)'Additional eostt may apply, consult a project manager for details. 'EMA reserves the right to return any samples tteldo not match our waste profile. Note: By telinquishbig samples to EnviroMitrix Analytical, IDC., client agrees to pay for the services requested on Ibis chain of custody form and any additional analyses performed on this project Payment Tor services is due within 30 days from the date of (he invoice. Sampte{») will be disposed of after 30 days unless otherwise noted. All work is subject to EMA's terms and conditions. ? f • r *-•«•• I California 5550 Mocehouse Drive Suite 150 San Dfego.CA 92121 858-587-7333 fax:858-587-6769 Washington 5009 Pacific Highway East Suite 2 TacomctWA 98424 253422-4296 fax:253-922-5814 British Columbia 8664 Commerce Court Bumaby, British Columbia V5A4N7 6OM03-9381 fax:60W03-9381 Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Testing Period: June 2008 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 August 19, 2008 Data Quality Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified By:.Date: f-rt- TOXICITY SUMMARY REPORT Test ID Number: 0806-S056,57,58 and 59 Client: Dudek & Associates Monitoring Period: June 2008 Sample Information Client: Dudek & Associates, Inc. Project Study: Carlsbad Golf Course Toxicity Evaluation - June 2008 Sample Site ID's: a) Downstream b) Upstream c) Well #3 d)Well#1 Sample Collection Date, Time: 6/11 /08,11:15 to 12:45 Sample Receipt Date, Time: 6/12/08,10:10 Sample Material: Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test End points: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Controls: Protocol Used: Test Acceptability Criteria: Test Validity: 6/12/08 to 6/19/08 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, < 8 hours 100, 50,25,12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity =» 200 uS/cm Lab freshwater spiked with seawater to raise the conductivity to levels matching the samples (range: 2,969 to 4,930 uS/cm). These controls were conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic EPA/821/B-00/004,2000 Method Guidance The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Test results met all test acceptability criteria and internal QA/QC requirements. Table 1. Water Quality Information: Samples Upon Receipt Sample ID Downstream Upstream Well #3 Well #1 PH 7.88 7.88 6.80 6.20 DO (mg/L) 9.1 10.1 5.7 5.4 Temp <°C) 2.9 2.9 2.9 2.9 Conductivity (uS/cm) 3559 3689 4930 2969 Hardness (mg/L as CaCO3) 1099 1172 1694 886 Alkalinity (mg/L as CaCOj) 285 259 262 90 Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: 0806-S056,57,58 and 59 Monitoring Period: June 2008 Table 2. Summary of Test Results Sample ID Downstream Upstream Well #3 Well #1 Concentration (%) Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Lab Control 6.25 12.5 25 50 100 Conductivity Control Mean Percent Survival 100 100 100 100 100 100 80 100 100 100 90 100 100 90 100 100 90 100 90 70 90 100 90 100 100 88 90 80 Mean Number of Offspring Produced 21.9 24.7 21.7 22.9 22.3 17.3 5.9* 20.2 20.4 17.8 19.2 19.2 11.6* 8.4* 21.2 22.1 23.4 18.8 10.7* 2.7* 3.6* 31.5 32.5 28.3 28.4 21.4 10.5* 22.5* * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Note: the Conductivity Control with each test had a conductivity level, which matched that of the 100% sample material. Nautilus Environmental - San Diego Btoassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: 0806-S056,57.58 and 59 Monitoring Period: June 2008 Table 3. Summary of Statistical Results and Reportab/e Values Sample ID Test & Endpoint Downstream: Survival Reproduction Upstream: Survival Reproduction Well #3: Survival Reproduction Well#1: Survival Reproduction NOEC (% effluent) 100 100 100 50 100 25 100 50 ECgo (% effluent) >100 >100 >100 >100 >100 48.7 >100 74.7 TUc (toxicity units) 1.0 1.0 1.0 2.0 1.0 4.0 1.0 2.0 NOEC = the highest Concentration that produces No Observed Effect ECso = concentration that causes an adverse effect to 50% of the test organisms Chronic Toxicity Units (TUc) = 1004 NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as welt as alt internal requirements under our Quality Assurance Program. In all four of the samples, there were no adverse effects observed with the survival endpoint. With regard to the reproduction endpoint, the Downstream site showed no adverse effects. However, for the other three sites toxicity was observed for this endpoint. The Upstream and Well #1 samples resulted in toxicity at the 100% concentration only, while Well #3 resulted in toxicity down to the 50% concentration. The adverse effects to reproduction were expected however, due to the elevated levels of conductivity found in the samples. Through internal studies and experience, we have consistently observed adverse effects to the reproduction endpoint when conductivity levels exceed approximately 2,500 uS/cm. Conductivity is related to the concentration of salts in a sample. The optimum range for Ceriodaphnia is between approximately 100 and 1,500 uS/cm. The correlation between higher conductivity levels and decreased reproduction in Ceriodaphnia was observed in this study as well. Since all four samples had conductivity levels greater than 2,500 uS/cm (ranging from 2,969 to 4,930 uS/cm), an adverse effect to the reproduction was expected. The Well #3 sample had the highest conductivity level (4,930 uS/cm), and as expected, showed the greatest decrease in the number of offspring produced. A Conductivity Control was tested along with each sample. The Conductivity Control consisted of standard laboratory water amended with natural seawater added to achieve a conductivity level equal to that of the 100% sample. Three of the Conductivity Controls, corresponding with the Upstream, Well #1, and Well #3 samples, resulted in similar reductions in the reproduction rate. However, the Conductivity Control Nautilus Environmental - San Diego B/oassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test IP Number 0806-S056,57,58 and 59 Monitoring Period: June 2008 corresponding with the Downstream sample showed a toxic response, while the sample itself showed no toxicity. in addition, the Well #1 sample showed a greater toxic response compared to the Conductivity Control. This might indicate that there was another factor influencing the toxic response, in addition to the high conductivity levels. Overall, though, the results of this study suggest that the elevated conductivity levels found in the samples may have contributed to the adverse effects observed in reproduction of the Ceriodaphnia test species. Nautilus Environmental - San Diego Bioassay Laboratory Appendix A Chronic Toxiclty Testing Raw Data and Statistical Analysis Site: Downstream CETIS Summary Report Report Date: Link/Link Code: 24 Jun-08 10:46 (p 1 of 2) 12-7554-741Q/0806-S056 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 03-5237-7043 Test Type: Reproduction-Survival (7d) Start Date: 12 Jun-08 15:00 Protocol: EPA/821/R-02-013 (2002) Ending Date: 19 Jun-08 15:30 Species: Ceriodaphnia dubia Duration: 7d 1h Source: In-House Culture Sample No: 06-4984-2488 Code: 08-0583 Sample Date: 1 1 Jun-08 11:15 Material: Downstream Ambient Sample Receive Data: 12 Jun-08 10:10 Source: Dudek & Associates Sample Age: 28h(2.9'C) Station: Downstream Comparison Summary Analysis No Endpoint 09-7053-2521 7d Survival Rate 12-4434-6370 Reproduction Test Acceptability Analysis No Endpoint NOEL LOEL 100 > 100 100 >100 Attribute 09-7053-2521 7d Survival Rate 12-4434-6370 Reproduction 12-4434-6370 Reproduction 7d Survival Rate Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Count 10 9 9 10 10 10 10 Count 10 9 9 10 10 10 10 Control Resp Control Resp PMSD Mean 0.8 1 1 1 1 1 1 Mean 5.9 21.9 24.7 21.7 22.9 22.3 17.3 95% LCL 0.643 1 1 1 1 1 1 95% LCL 3.85 19.8 22.7 19.8 20.1 20.2 15.3 TestStat 1 21.9 0.292 95% UCL 0.957 1 1 1 1 1 1 95% UCL 7.95 24 26.6 23.6 25.7 24.4 19.3 TOEL N/A N/A Nautilust Environniwiiai \w\j Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Age: <Bh Client Dudek & Associates Project PMSD N/A 29.2% Method Rsher Exact/Bonferroni-Holm Test BonferroniAdjtTest Acceptabitty Limits Overlap 0.8 - NL 15 -NL 0.13-0.47 Mln 0 1 1 1 1 1 1 Min 0 14 14 11 9 11 8 Max 1 1 1 1 1 1 1 Max 18 32 29 29 32 30 24 Yes Yes Yes StdErr 0.077 0 0 0 0 0 0 StdErr 1 1.04 0.953 0.915 1.35 1.03 0.985 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria StdDev 0.422 0 0 0 0 0 0 StdDev 5.49 5.71 5.22 5.01 7.37 5.64 5.4 CV% 52.7% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% cv% 93.0% 26.1% 21.2% 23.1% 32.2% 25.3% 31.2% Diff% 0.0% -25.0% -25.0% -25.0% -25.0% -25.0% -25.0% Diff% 0.0% -271.0% -318.0% -268.0% -288.0% -278.0% -193.0% 000-089-163-4 CETIS™ v1.6.3revE CETIS Summary Report Report Date: Link/Link Code: 24 Jun-08 10:46 (p 2 of 2} 12-7554-7410/0806-S056 Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 1 Rep1 7 22 19 18 23 11 10 Rep 2 1 1 1 1 1 1 1 Rep 2 6 18 28 24 25 20 18 Rep 3 0 1 1 1 1 1 Rep 3 0 32 19 12 25 24 Rep 4 1 1 1 1 1 1 1 Rep 4 2 24 28 26 9 30 21 Rep 5 0 1 1 1 1 1 1 Rep 6 0 16 28 29 20 29 12 Rep 6 1 1 1 1 1 1 1 Rep 6 10 26 22 11 30 25 18 Rep 7 1 1 1 1 1 1 1 Rep 7 18 19 14 21 25 23 20 Rep 8 1 1 1 1 1 1 1 Rep 8 4 26 28 25 27 20 8 Rep9 1 1 1 1 1 1 1 RepS 9 14 26 23 26 17 21 Rep 10 1 1 1 1 1 1 Rep 10 3 29 21 32 23 21 000-089-163-4 CETIS ™v1.6.3revE Analyst: CETIS Analytical Report R«P°rt D** Link/Link Code: 24 J""-08 10:48 <P 1*1) 12-7554-741 0/0806-S056 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 09-7053-2521 Endpolnt 7d Survival Rate CETIS Version: CETISv1.6.3 24 Jun-08 10:45 Analysis: STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% TestStat P-Value Decislon(O.OS) Lab Control 6.25 1 1 Non-Significant Effect 12.5 1 1 Non-Significant Effect 25 1 1 Non-Significant Effect 50 1 1 Non-Significant Effect 100 1 1 Non-Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp 1 0.8 -NL Yes Passes acceptability criteria Data Summary Conc-% Control Type No-Resp Resp Total 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- fO.8: 0.7- 3 0.5- 5 o.. 0.* 0.1- 0.0 Lab Control 909 909 10 0 10 10 0 10 10 0 10 10 0 10 e e • e e e 0 6.25 US 25 50 100 Conc-% 000-089-163-4 CETIS™ v1.6.3revE Analyst:QA: CETIS Analytical Report Report Date: Link/Link Code: 24 Jun-08 10:46 (p 1 of 1) 12-7554-7410/0806-S056 Ceriodaphnla 7-d Survival and Reproduction Test Analysis No: 12-4434-6370 Analyzed: 24 Jun-08 1 0:45 Endpoint: Reproduction Analysts: Parametric-Multiple Comparison Data Transform Zeta Alt Hyp Monte Carlo NOEL Untransformed Bonferroni Adj tTest Control vs Conc-% Lab Control 6.25 12.5 25 50 100 Test Acceptability Attribute Control Resp PMSD OT Not Run 100 Test Stat Critical MSD P-Value -1.02 2.4 6.55 1.0000 0.071 2.4 6.38 1.0000 -0.38 2.4 6.38 1.0000 -0.155 2.4 6.38 1.0000 1.73 2.4 6.38 0.2260 Test Stat Acceptability Limits Overlap 21.9 15 -NL Yes 0.292 0.13-0.47 Yes ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Between 291.0318 Error 1742.089 Total 2033.121 ANOVA Assumptions Attribute Test 58.20636 5 1.74 0.1420 33.50171 52 91.70807 57 Test Stat Critical P-Value Variances Bartlett Equality of Variance 1.76 15.1 0.6810 Distribution Shapiro-Wilk Normality 0.949 0.0156 Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Mfn 0 Lab Control 9 21.9 19.7 24.1 14 6.25 9 24.7 22.7 26.7 14 12.5 10 21.7 19.8 23.6 11 25 10 22.9 20.1 25.7 9 50 10 22.3 20.2 24.4 11 100 10 17.3 15.2 19.4 8 Graphics 40-, S 30~ 1 ~*~ £ 20- 10- 0 6.25 is: ifr 1 1 1 — 1 1 •*" "*~ ^ 11+ I °5 Rejc ct Hi B -10- Nautilus Environmental (CA) CETIS Version: CETISv! .6.3 Official Results: Yes LOEL TOEL TU PMSD >100 N/A 1 29.2% Declsion(S%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Declsion(5%) Non-Significant Effect Declsion(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 32 1.06 5.71 26.1% 0.0% 29 0.969 5.22 21.2% -12.7% 29 0.931 5.01 23.1% 0.86% 32 1.37 7.37 32.2% -4.62% 30 1.05 5.64 25.3% -1.88% 24 1 5.4 31.2% 21.0% /<* •rftf** ~^f /"»••• • 12.5 25 SO 100 -2.5 -2.0 -1.5 -1.0 -0.5 O.D 0.5 1.0 l.S 2X1 2.5 Conc-% Rankits 000-089-163-4 CETIS™ v1.6.3revE :reshwater Chronic Bioassay lent/Sample ID: Test Number: Dudek-Carlsbad GC Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 6/12/2008 End Date/Time: 6/19/2008 Cone. LC AnaVj Verlf Rep 1 2 3 4 5 6 7 8 9 10 /st edby: Board/ Cup •-H W *f *»R H& 1 tt 3t-^1 2*1£onH **-$&- Daily Reproduction/ Survival d 0 -ffo 0 0 6 0 0 O f>& mmm& • o 00oo fv tJ 0 b CS Wig!6oo O O 0o G O & TC 00 0 0\ Qo 0 0 0 Nr\ !j:i!i!f!l* ^T (0 2 & ^«, W -5tip Y5" SSRiSii •5 •f H I/8 -7 /«/ (£>1 S>K/ *;;!*$*; Is*Oia-ii-7i^/oid-/?— Y^C- ^ii;i8;i;i;ii — Total 1/IS P\1.v\ ^-jjp 1*1 «^IF QC M *& "H me Fed/Test Solution Renewed (day): Ml Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 • i Daily Reproduction/ Survival 3!l::::j:i d O 00 0 0 Q$ 9,0 mmm# •£> 0 0 A •o D O O t> IliifilSsiriii 0 £» O O /9 ^O o P>U *3#ji;ij Qp(/ 0 00u 09 fl !ii!!;5il!!:!i ^ ^/D ^LAM*?<-t?>. ¥^> IliiHIBi:!!!:; M -/G/d O M— £ 6, 2. 5 1 |:-i-::Ti:!::::e> t3^ n—o¥v 6 0 r^ ;i;ii!i8Ji;;i;i _ — Total ^> lr» 0/d ^Ole JO' l« J?1 T> Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Dally Reproduction/ Survival 0 0 £0 0 $0 0 0o :!!!!!£!£!; O £> 0o O 05 £> O O !li^i?i!!!!J O £O C1 /5 ft ft & Oo !:!i|i|4-:|:l: D4P0 %6 5 ^u-fl i!i!ii5!ii:|i O O H 5 O oWooft :i:|:J8|:!:|:i -7 ^10 X9 lo -7 l-2_to1 ;:-:::i7;:;:;:: l^ I ^ \01ip »5Hi^^ca l>l>- loJ- !&:$!i!!i;Total 11 IM-JUf is ^?vV 4 IXy*if\ Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Dally Reproduction/ Survival ..:ip!iiij!6& 000 0 0 0 0 \D ilililiZhl!;!o C> 6 Oo /o *} /-> O •«n ;i!!i!l3!=i!!! O Oo O Oft f\ D A U iiiiiiiftilii 0- o \ *> i^ 0 \ 1•«i 0 !!!!!!!Sli!i!=«i •S O o O Wo O fii=!=Sl!i!!jj «f f Y ^ ft -7 7 13 ft# !ii!!!i?!ii[H? », o ^< te NQ ^i ,1^-o ii^i!8Ji!;i|!Total us_2i r,M 2*?x,1jlxl ?TlA2^ QC O QC ID QC IS lotes: d ~ dead; M " male; LIP = lost In progress; B • 4th brood (only the first 3 broods are included In total) imments: 'C Check:Final Review: *?£ lautilus Environmental 5550 More/rouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek-Carlsbad GC Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 6/12/2008 End Date/Time: 6/19/2008 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup Ml W ^ ( ?*>\o 1 ^V & }<( ifb H M ^£(, Daily Reproduction/ Survival *;;#*:;; ft0 0 6 <?0 (3 0 0 trV SilliiailiiH: o o o O D » C? 0 o O ES fllilfilim 6 O O 0n /o<? o o 0 r*: I*!!*];!* «tD f? b2- } *Cl •^% Mft !;ii!:£!ii!ii O 3 6 O 0<•? I o & 0 to^ wmms^ J2-i^> 7 tl |o 8 tl (4 *W .i!l!^(S!i!J|l \\ \-** 5. S3,M>b to 1419- 15 to. llliJlSHiiH!Total 2-3 1& \l~ 7J\ ID -50 l£ tt Tl* 32- QC 15 Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival . . . HUH!!!! 03 0 0 0 Q 0 0 0 0 !!i!=!S!!!!$ ^5 7~> O Q O Do (~> o Q w$mo 0o fi r> 0Oo 0n !ii!iii*Jl:ilii 1- 2. ^4 !t?>\\o ^o 0 f> £> O O O O ^ liilsiiKHiili 7 £ 1 1 lo> ey ^* 1 ^ liiiTjiil 0, 13- II It* 15 H3, Wi^. (0 Ijliil^iipji Total \l ^0 ^^04.1^$ 2,3- 2-0 l"-r- 7^ QC N Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival I!*!!!!!!6 0 0 0 0 0 0 0 (20 Illl!!*!!];!- f) o ft n r,y ^Q C7 U ^>O5o 06 O ooo 0 ^*IH5>4M-0 Vo 0 / oo«oI p.s D^iiPiiH^11-7 0 11 o1 (o mmm D | 1 & <AMl £ntoJo !i!i!!j*!il Total ID I}? 2*1 U 12- i? 7^) « 1\ 14 QC /o Time Fed/Test Solution Renewed (day): (11 tai (51 (7) Notes: d«dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Check:Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client Dudek-Carlsbad GC Sample ID: Test No: Water Quality Measurements Test Species: C. dubla Start Date/Time: mvxx» End Date/Time:. Conductivity Control Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: "DF A MD B:Sample Log-ln Numbers: A; QCCheck: Nautilus Environmental 5550 Moreftoi/se Drive, Su/te f 50. San Diego, CA 92121. Animal Age at Initiation: - C: Final Review: Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek-Carlsbad GC Sample ID: Test No: Test Species: C. dubia Start Date/Time: End Date/Time: Sample Used (A, B. C): Comments: Animal Source/Date Received: Sample UQ3,-ln Numbers: A: QC Check: //A B: Animal Age at Initiation: C: Final Review: NwUlus Environment*! 5550 Manhamt Drive, Suite f 50. S»n OM0O. CA 92f 2f. Site: Upstream CETIS Summary Report Report Date: Link/Link Code: 24Jun-0809:27(p1of 2) 17-0693-1544/0806-S057 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 02-9187-8707 Test Type: Reproduction-Survival (7d) Start Date: 12 Jun-08 15:00 Protocol: EPA/821 /R-02-01 3 (2002) Ending Date: 19 Jun-08 13:05 Species: Ceriodaphnia dubia Duration: 6d 22h Source: In-House Culture Sample No: 01-3112-6794 Cc Sample Date: 11 Jun-08 12:00 Mi Receive Date: 12 Jun-08 10:10 So Sample Age: 27h(2.9'C) St Comparison Summary Analysis No Endpoint 06-6730-1835 7d Survival Rate 03-9182-3588 Reproduction ^Cwiro 07-8782-0580 (iskCMfak Point Estimate Summary Analysis No Endpoint 06-6921-3869 Reproduction Test Acceptability Analysis No Endpoint de: 08-0584 urce: Dudek & Associates itton: Upstream >£"&) NOEL LOEL 100 > 100 <0 0 50 100 Effect-%Conc-% 25 74.5 50 > 100 Attribute 06-6730-1635 7d Survival Rate 03-9182-3588 Reproduction Reproduction 06-6921-3869 Reproduction 07-8782-0580 Reproduction 03-9182-3588 Reproduction 07-8782-0580 Reproduction 7d Survival Rate Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Count 10 10 9 10 10 10 10 Count 10 10 9 10 10 10 10 Control Resp Control Resp Control Resp Control Resp Control Resp PMSD PMSD Mean 0.9 1 1 1 0.9 1 1 Mean 8.4 20.2 20.4 17.8 19.2 19.2 11.6 95% LCL 0.782 1 1 1 0.782 1 1 95% LCL 6.69 18.2 17.6 15.6 16.4 17.2 9.72 TestStat 1 20.2 8.4 20.2 20.2 0.194 0.327 95% UCL 1 1 1 1 1 1 1 95% UCL 10.1 22.2 23.3 20 22 21.2 13.5 Nautilus Environmental (CA) Analyst: Diluent: Not Applicable Brine: Not Applicable Age: <8h Client Dudek & Associates Project: TOEL N/A N/A 70.7 PMSD N/A 19.4% 32.7% Method Fisher Exact/Bonferroni-Holm Test Equal Variance t Two-Sample Test Wilcbxon/Bonferroni Adj Test 95% LCL 95% UCL Method 35.8 N/A 91.1 N/A Linear Interpolation (ICPIN) Acceptabitty Limits Overlap 0.8 - NL 15 -NL 15 -NL 15 -NL 15 -NL 0.13-0.47 0.13 - 0.47 Min 0 1 1 1 0 1 1 Min 0 13 8 10 6 11 3 Max 1 1 1 1 1 1 1 Max 15 28 27 26 28 28 17 Yes Yes Yes Yes Yes Yes Yes StdErr 0.0577 0 0 0 0.0577 0 0 StdErr 0.835 0.999 1.38 1.06 1.35 0.988 0.92 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria StdDev 0.316 0 0 0 0.316 0 0 Std Dev 4.58 5.47 7.55 5.79 7.41 5.41 5.04 CV% 35.1% 0.0% 0.0% 0.0% 35.1% 0.0% 0.0% CV% 54.5% 27.1% 36.9% 32.5% 38.6% 28.2% 43.4% Diff% 0.0% -11.1% -11.1% -11.1% 0.0% -11.1% •11.1% Diff% 0.0% -140.0% -143.0% -112.0% -129.0% -129.0% -38.1% vtt« -h? 000-089-163-4 CETIS™ v1.6.3rev£Analyst: CETIS Summary Report Report Date: Link/Link Code: 24 Jun-08 09:27 (p 2 of 2) 17-0693-1544/0806-S057 Ceriodaphnta 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail , Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 1 1 1 0 1 1 Rep 2 1 1 1 1 1 1 1 Rep 3 1 1 1 1 1 1 1 Rep 4 1 1 1 1 1 1 1 Rep 5 1 1 1 1 1 1 1 Rep 6 1 1 1 1 1 1 1 Rep 7 1 1 1 1 1 1 1 RepS 1 1 1 1 1 1 1 Rep 9 1 1 1 1 1 1 1 Rep 10 0 1 1 1 1 1 1 Reproduction Detail Conc-% Q 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 11 14 13 6 13 5 Rep 2 10 26 8 10 27 22 10 Rep 3 6 14 9 12 22 11 12 Rep 4 5 28 20 26 24 20 14 RepS 11 13 25 11 26 14 13 Rep 6 4 22 27 23 28 21 17 Rep 7 15 22 27 21 17 25 17 RepS 9 24 25 20 11 28 17 Rep 9 13 23 26 23 14 17 3 Rep 10 0 16 17 19 17 21 8 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 24 Jun-08 09:27 (p 1 of 1) 1 7-0693-1 544/0806-8057 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 06-6730-1835 Endpoint: 24 Jun-08 9:26 Analysts: 7d Survival Rate CETIS Version: CETISvl.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Cone-% TestStat P-Value Decislon(0.05) Lab Control 6.25 1 12.5 1 25 0.5 50 1 100 1 Test Acceptability Attribute Test Stat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- J0.8- 0.7 | 0.6- 3 O.S- ** 0.4- 0.3- 0.2- 0.1- 0.0 Lab Control 10 0 9 0 10 0 9 1 10 0 10 0 e e • 0 6.25 1J.S 25 Conc-% 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 9 10 10 10 10 • e so too 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 24 Jun-08 09:27 (p 2 Of 3) 17-0693-1544/0806-S057 Coriodaphnia 7-d Survival and Reproduction Test Analysis No: 07-8782-0580 Analyzed: 24 Jun-08 9:26 Endpoint: Reproduction Analysis: Nonparametric-Multiple Comparison Data Transform Zeta Rank Wilcoxon/Bonferroni Adj Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100- Test Acceptability Attribute Control Resp PMSO Alt Hyp OT Monte Carlo 10000 Tria Test Stat Critical 95.5 89 105 96 69.5 Ties 1 3 5 4 2 TestStat Acceptability Limits 20.2 15 -NL 0.327 0.13-0.47 ANOVA Table Source Sum Squares Mean Square Between 540.91 Error 2013.022 Total 2553.932 ANOVA Assumptions Attribute Test 108.182 37.98155 146.1635 Variances Bartlett Equality of Variance Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Rank Transformed Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Count 10 9 10 10 10 10 Count 10 9 10 10 10 10 Mean 20.2 20.4 17.8 19.2 19.2 11.6 Mean 35.6 36.4 28.3 33.7 32.3 14.4 OF 5 53 58 Test Stat 2.56 0.945 95% LCL 18.1 17.6 15.6 16.4 17.1 9.68 95% LCL 29.9 28.8 22.3 26.3 26.7 10.4 FStat 2.85 Critical 15.1 95% UCL 22.3 23.3 20 22 21.3 13.5 95% UCL 41.2 44.1 34.3 41 37.9 18.4 NOEL 50 P-Value 1.0000 0.4620 1.0000 1.0000 0.0100 Overlap Yes Yes P-Value 0.0238 CETIS Version: Official Results LOEL 100 TOEL 70.7 Nautilus Environmental (CA) CETISvl : Yes TU 2 6.3 PMSD 32.7% Decfsion(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Declsion(5%) Significant Effect P-Value Decisfon(1 %) 0.7670 0.0097 Mln 13 8 10 6 11 3 Mln 15.5 4.5 7.5 3 10 1 Equal Variances Non-normal Distribution Max 28 27 26 28 28 17 Max 58 55 51.5 58 58 27 StdErr 1.02 1.4 1.07 1.38 1 0.935 StdErr 2.76 3.73 2.93 3.56 2.73 1.95 Std Dev 5.47 7.55 5.79 7.41 5.41 5.04 Std Dev 14.9 20.1 15.8 19.2 14.7 10.5 CV% 27.1% 36.9% 32.5% 38.6% 28.2% 43.4% CV% 41.8% 55.1% 55.7% 57.0% 45.5% 72.9% Diff% 0.0% -1.21% 11.9% 4.95% 4.95% 42.6% Diff% 0.0% -2.52% 20.4% 5.34% 9.14% 59.5% 000-089-163-4 CETIS™ v1.6.3revE Analyst:,QA: CET1S Analytical Report Report Date: Link/Link Code: 24 Jun-08 09:27 (p 3 of 3) 17-0693-1544/0806-S057 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 07-8782-0580 Analyzed: 24 Jun-08 9:26 Endpointe Reproduction Analysis: Nonparametric-Multiple Comparison CETIS Version: CETISvl .6.3 Official Results: Yes Graphics 30- 25- 20- 15- 10- ReMctmlU 30: M-. 10- 0 •10- -20: •30- 6.25 12.5 15 Conc-% 50 100 -2.5 -2.0 -1.5 -1.0 -OS 0.0 OS 1.0 1.5 2.0 2.5 RanUts 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 24 Jun-08 09:27 (p 1 of 1) 17-0693-1544/0806-S057 Ceriodaphnla 7-d Survival and Analysis No: 06-6921-3869 Analyzed: 24 Jun-08 9:27 Linear Interpolation Options X Transform Y Transform Linear Linear Test Acceptability Attribute Control Resp Reproduction Test Endpoint Analysis: Seed 7055475 TestStat 20.2 Reproduction Linear Interpolation (ICPIN) Resamples 280 Acceptability 15 -NL CETIS Version: Official Results: Nautilus Environmental (CA) CETISv1.6.3 Yes Exp 95% CL Method Yes Limits Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95%LCL 95% UCL 25 74.5 35.8 50 > 100 N/A Reproduction Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Reproduction Detail Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Graphics 25- »i-V*-' 1 15- S 10- 5- 0 20 td 91.1 N/A Calculated Variate Count Mean 10 20.2 9 20.4 10 17.8 10 19.2 10 19.2 10 11.8 Rep 1 Rep 2 14 26 8 9 23 19 6 27 13 22 5 10 -•. ^X. 60 80 Conc-% Min 13 8 10 6 11 3 Rep 3 14 20 13 22 11 12 X, 100 120 Uax 28 27 26 28 28 17 Rep 4 28 25 10 24 20 14 StdErr 0.999 1.38 1.06 1.35 0.988 0.92 Rep 5 13 27 12 26 14 13 Std Dev 5.47 7.55 5.79 7.41 5.41 5.04 Rep 6 22 27 26 28 21 17 CV% 27.1% 36.9% 32.5% 38.6% 28.2% 43.4% Rep 7 22 25 11 17 25 17 Diff% 0.0% -1.21% 11.9% 4.95% 4.95% 42.6% Rep 8 Rep 9 Rep 10 24 23 16 26 17 23 21 20 11 14 17 28 17 21 17 3 8 000-089-163-4 CETIS™ v1.6.3revE CETIS Analytical Report vX Report Date: Link/Link Code: 24 Jun-08 09:27 (plof 3) 17-0693-1544/0806-S057 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 03-9182-3588 Endpoinfc Reproduction 24 Jun-08 9:26 Analysis: Parametric-Two Sample Data Transform Zeta Alt Hyp Monte Carlo NOEL Untransformed C>T Not Run <0 Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Lab Control Conductivity Control 5.23 1.73 3.91 0.0000 Test Acceptability Attribute TestStat Acceptability Limits Overlap Control Resp Control Resp PMSD ANOVA Table Source Between Error Total 20.2 8.4 0.194 15-NL Yes 15 -NL Yes 0.13 - 0.47 Yes Sum Squares Mean Square DF FStat P-Value 696.2 696.2 1 27.4 0.0001 458 25.44444 18 Nautilus Environmental (CA) CETIS Version: CETISvl.6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 19.4% Decislon(5%) Significant Effect Decision Passes acceptability criteria Passes acceptability criteria "Wjo^y <s \>ec>\ Declsion(5%) Significant Effect 1154.2 721,6445 19 ANOVA Assumptions Attribute Test Variances Distribution Variance Ratio F Shapiro-Wilk Normality TestStat Critical P-Value 1.43 6.54 0.6020 0.941 0.2530 Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Min 0 0 Graphics 30- 25- « 15- 10- 5- Lab Control 10 20.2 18.1 22.3 13 Conductivity Co 10 8.4 6.66 10.1 0 0 /v> Conc-% 8- 6- * II *I! « •4- -6- Re*ctNuO .g. Declslon(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Ditr% 28 1.02 5.47 27.1% 0.0% 15 0.85 4.58 54.5% 58.4% s- • /» * 0 -2.0 -13 -1.0 -0.5 0.0 0.5 1.0 l.S 2.0 000-089-163-4 CETIS™ v1.6.3revE Analyst:.QA: Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D.magna lient/Sai TesU •nple ID: J umber: Cone. LC Dudek-Carlsbad GC U0&Vf ?a<V\ fi^CIW Start Datemme: 6/12/2008 )-S<3O £*>*•? "T" ' End Date/Time: 6/19/2008 \-^o-^ Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup tv> ^ "ff ^ in 35 -%<tf ft"! 2f^Mofe>jO 5§> to p£y £L_ Daily Reproduction/ Survival :::::::J::;:::.: o Q Q u A ll 50re •:::::-2:::::: 0 oo o 0 0 o 0 0 o ££ •••:•••;••$:•••••• O&oo op5o6nr*r :-:::-4:;:::: (^> 0 o o ^tt d &- Of.j :::i;:S-::-:: q- cre:U ^C"o o 0y MU :::!::$::::::; T ' '} A^ff g Jl t] 1 \-lf-7-- :•:•:• 0to 0 \2_ ' 13-c\ IO ' *T^^£fK •:::i:ift:i:i:i Total \u Oi* JU 1& 16 2/2, •m "2&~ |k QC M £<, Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival :::=:1j:!:j:j 9 0eo (So 0 0 0o !#!#&£! O O © O o 0o e> few j!!!H:a;!j!;! O O o 0r>o 0 0 O O #&w 0o0/•>0 0 0o QOld iil!i!!5ll;!!i; < % O (J> n 0 •7j 0— mwm\e 7 £yAf H6si— 8&S8S O 0c> 0-7 0•^o<^ — ;=!!i!i8j;!:!!! — Total l\ tn \o 9 \\ (4- \CJ \is oAA QC -> <* Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival !:;#!!!*£ ft 0 0 Q& QdO 0b ;*;;;2i:!* O e> 0oo oo <D Oo $mm %ftp 00 06 •^;:!4s-;i: o0o *¥f2~ 0 O V M ::|i:;5gn|i; i7P q n^on Ii rj, A 0 d m$m_*yj^ '*?M^yJPw ;::;;: J7j:|:j:; - O O 1C?mloi\^m 13a ;;!iH«|;|ii;~ Total Uf ?? ^70 2&1&r W 2&A? IT- QC OfX Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Dally Reproduction/ Survival !={•!#£=:: ft O <?<!> O (5 ft I) ) O IHifjpllii•""P" '"' O rt rt o o & 0 no m&mi6c> f5 OP)on G Oc) !=l!ii!*!i!i! '<*"" O 0 3. £> ^» ^*3 M- d. iili!i!5li!;i! H' P ^ft' ? O n0 O /7) iliHiSiliili; '^IO 1! \7 t ID IP ^•f * lWi7;;;;;;: ^0 0noy?#^i°\\ i!i!:;«;i:i;i Total T3 IO I7-iun ^3 Z-lzo *#l< QC I OP<, . Time Fed/Test Solution Renewed (day): Notes: d = dead; M = male; LIP = lost in progress; B « 4th brood (only the first 3 broods are included In total) Comments: QC Check:Final Review: Q& Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek-Carisbad GC Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D.pulex D.magna Start Date/Time: 6/12/2008 IS a O End Date/Time: 6/19/2008 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup 431 i\l til "V v-7 35 %W "313rt 40 100 5*t/ 4fr k£t_ Daily Reproduction/ Survival iiisS-H:!!: O £) ft 0oo 0ftft o o c 0 0 0 0 o 0 0 c<, o o o 00o ooo 0 TDf* iiiiiiSiiiii! Oo QJ9^ 0 3) O O 0 <h *i& <bc'if <? *L- 0 1 l^00 "fti iiii=i*!i!i!lo//> \07ff \d \\ 7 4 V f>K — \3. l\ l3 IH- 14' o o ^-lf^ iiiiiiiSiiiiii^ Total WA 7^*22- 2*4 £b 1% \' \ \l I f •f- QC &> ^s Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Dally Reproduction/ Survival . iillili!!! O O <D 0 90 0ft&o o 0 o 0 o o O 6 0 c) 0» o £> ft D 0(7 Qv HliJIJSHii!!! O 0 £> 2. 3- ^> M- O 3- ft mmm1 34fti\ 0]Vdfi msmtoy 7t V1pIff •7$ !!!!:ii?iii!ii 0 u D^o JO .31 13 7 to liiiiiigiiiiii Total (^ O-'i-n 2X? \ li xi •2b 28n-2-\ QC ?£5 Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival i & & O (0 oo 0 0 0 0 oo o 6 o O 0o o c) !!i=i;3;Ni!i;6 0noo©c> o 96 ;#!;*!!*: o "c> O O O& 0 0e> o =!*;&!£! J2mH £ M«•-\ 7.*r 0^ !i!Hi!«i!=iii 3 k 5 10 o £, O & j"» 7 iliiiJiSn::!::-o f3 -I O ^ ^10 <=!o O !!!ii=i$i;!iii Total 5 O 12- IM- \2» \T- ^JOf ^S QC ^\ £5 Time Fed/Test Solution Renewed (day): (0) \3o* fH i3'.HT (2ULIO (3)_ Notes: d = dead; M = male; UP = lost In progress; B = 4th brood (only the first 3 broods are included In total) Comments: QC Check:Final Review: Nautilus Environmental 5550 Morehouae Drive, Suit* 150. San Dfogo, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek-Carlsbad GC Sample ID: Test No: Test Species: C. dubia 08 Start Date/Time: <unaa» End Date/Time: < Concentration Lab Control Day r 3 r T Conductivity Control Afi. A Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: Sample Log-In Numbers: _A: QC Check: Nautilus Environmental SSSOMorehouse Drive, Suite 150. San Diego, CA 92121. MO Animal Age at Initiation: C: Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek-Carlsbad GC Sample ID: ' Test No: Test Species: C.dubla Start Date/Time: End Data/Time:: (ait^l&ft Final: Sample Used (A. B, C): Comments:. Animal Source/Date Received: _ Sample Log-in Nun QC Check: Animal Age at Initiation: — C: • Final R«««w:_£L6_JL42!l|j2i. Nurtlus EnvironmtMal S5SO Monhoute Drivt, Sufte 150. Swi Dhgo, CA tUtl. Site: Well 3 CETIS Summary Report Report Date: Llnk/Unk Cod*: 24Jun-0810:56(p1of 2) 07-3146-7449/0806-S058 Ceriodaphnta 7-d Survival and Reproduction Test Test Run No: 16-7093-8188 Test Type: Reproduction-Survival (7d) Start Date: 12 Jun-08 15:55 Protocol: EPA/821 /R-02-01 3 (2002) Ending Date: 19 Jun-08 12:45 Species: Ceriodaphnia dubia Duration: 6d 21 h Source: In-House Culture Sample No: 03-9017-3510 Code: 08-0585 Sample Data: 11 Jun-08 12:30 Material: Groundwater Receive Data: 12 Jun-08 10:10 Source: Dudek & Associates Sample Age: 27h(2.9'C) Station: Well 3 Comparison Summary Analysis No Endpoint 21-3441-8225 7d Survival Rate 08-1890-8473 Reproduction fublMM v*. d*JC\ 10-1239-6682 0J»flrttaWjC &P£) Point Estimate Summary Analysis No Endpoint 20-3427-6000 Reproduction Test Acceptability Analysis No Endpoint NOEL LOEL 100 > 100 <0 0 25 50 Effect-% 25 50 Attribute 21-3441-8225 7d Survival Rate 08-1890-8473 Reproduction Reproduction 10-1239-6682 Reproduction 20-3427-6000 Reproduction 08-1890-8473 Reproduction 10-1239-6682 Reproduction 7d Survival Rate Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 6.25 12.5 25 50 100 Count 10 10 10 10 10 10 10 Count 10 10 10 10 10 10 10 Control Resp Control Resp Control Resp Control Resp Control Resp PMSD PMSD Mean 0.9 1 1 0.9 1 0.9 0.7 Mean 3.6 21.2 22.1 23.4 18.8 10.7 2.7 95% LCL 0.782 1 1 0.782 1 0.782 0.52 95% LCL 2.44 19.9 20.1 21.8 17.5 8.46 1.41 Conc-% 31.6 48.7 TestStat 1 21.2 3.6 21.2 21.2 0.123 0.216 95% UCL 1 1 1 1 1 1 0.88 95% UCL 4.76 22.5 24.1 25 20.1 12.9 3.99 TOEL N/A N/A 35.4 Nautilus Environmental (CA) Analyst Diluent Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client Dudek & Associates Project PMSD N/A 12.3% 21.6% Method Fisher Exact/Bonferroni-Holm Test Equal Variance t Two-Sample Test Dunnetfs Multiple Comparison Test 95% LCL 95% UCL Method 24 41.6 38.9 64.1 Linear Interpolation (ICPIN) Acceptably Limits Overlap 0.8 - NL 15 -NL 15 -NL 15-NL 15 -NL 0.13-0.47 0.13-0.47 Mfn 0 1 1 0 1 0 0 Min 0 15 12 12 12 0 0 Max 1 1 1 1 1 1 1 Max 9 26 28 28 23 19 9 Yes Yes Yes Yes Yes Yes Yes StdErr 0.0577 0 0 0.0577 0 0.0577 0.0882 StdErr 0.566 0.654 0.983 0.785 0.625 1.1 0.633 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Std Dev 0.316 0 0 0.316 0 0.316 0.483 Std Dev 3.1 3.58 5.38 4.3 3.43 6 3.47 CV% 35.1% 0.0% 0.0% 35.1% 0.0% 35.1% 69.0% CV% 86.1% 16.9% 24.4% 18.4% 18.2% 56.1% 128.0% Diff% 0.0% -11.1% -11.1% 0.0% -11.1% 0.0% 22.2% Diff% 0.0% -489.0% -514.0% -550.0% -422.0% -197.0% 25.0% h» 000-089-163-4 CETIS™ v1.6.3revE Ana.vst: ^QA CETIS Summary Report Report Date: Link/Link Code: 24 Jun-08 10:56 (p 2 of 2) 07-3146-7449/0806-8058 Certodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 1 Rep1 9 22 17 24 12 8 0 Rep 2 1 1 1 0 1 1 1 Rep 2 0 17 12 12 21 15 9 Rep 3 1 1 1 1 1 0 1 Rep 3 0 19 26 25 19 0 7 Rep 4 0 1 1 1 1 1 0 Rep 4 0 15 25 22 21 8 0 RepS 1 1 1 1 1 1 1 RepS 4 20 28 25 19 18 0 Rep 6 1 1 1 1 1 1 0 Rep 6 3 21 28 25 16 19 5 Rep 7 1 1 1 1 1 1 1 Rep 7 7 22 23 25 23 15 5 Rep 8 1 1 1 1 1 1 0 RepS 3 25 16 23 17 5 0 Rep 9 1 1 1 1 1 1 1 RepS 4 26 23 25 23 9 0 Rep 10 1 1 1 1 1 1 1 Rep 10 6 25 23 28 17 10 1 000-089-163-4 CETIS™ v1.6.3revE Analyst::!!**- CETIS Analytical Report Report Date: Link/Link Coda: 24Jun-0810:56(p1 of 1) 07-3146-7449/0806-S058 Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 21-3441-8225 Endpomfc 24 Jun-08 10:55 Analysis: 7d Survival Rate CETIS Version: CETISv1.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransfbrmed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferronl-Holm Test Control vs Conc-% TestStat P-Value Dectslon(0.05) Lab Control 6.25 1 12.5 0.5 25 1 50 0.5 100 0.105 Test Acceptability Attribute TestStat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9: 1 a* •I 0.6- 3 0.5: OJ- 0.2- 0.1: 0.0 Lab Control 10 0 10 0 9 1 10 0 9 1 7 3 • e e e 0 6.25 1ZS 25 Cooc-% 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 0.526 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 10 10 • e 50 100 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Ceriodaphnla 7-d Survival and Reproduction Test Analysis No: Analyzed: 10-1239-6682 24 Jun-08 10:55 Data Transform Endpoint: Reproduction Analysis: Parametric-Control vs Treatments Zeta Alt Hyp Untransfomned OT Dunnett's Multiple Comparison Test Control vs Conc-% Test Stat Lab Control 6.25 12.5 25 50* 100* Test Acceptability Attribute Control Resp PMSD ANOVA Table Source Between Error Total -0.45 -1.1 1.2 5.25 9.25 Monte Carlo NOEL Not Run 25 Critical MSD P-Value 2.29 2.29 2.29 2.29 2.29 4.58 0.9340 4.58 0.9880 4.58 0.3300 4.58 0.0000 4.58 0.0000 Test Stat Acceptability Limits Overlap 21.2 15 -NL 0.216 0.13-0.47 Sum Squares Mean Square 3304.283 1080.7 4384.983 660.8567 20.01296 680.8696 ANOVA Assumptions Attribute Test Variances Distribution Bartlett Equality of Variance Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count Mean 0 6.25 12.5 25 50 100 Graphics 30- 25- 1 ID- S' Lab Control 10 10 10 10 10 10 =•= 0 21.2 22.1 23.4 18.8 10.7 2.7 »- "*" DF 5 54 59 Yes Yes F Stat P-Value 33 0.0000 Test Stat Critical P-Value 5.26 0.967 15.1 0.3850 0.1080 95%LCL 95%UCL Min 19.8 20.1 21.8 17.5 8.42 1.38 22.6 15 24.1 12 25 12 20.1 12 13 0 4.02 0 llReject Null H. — i 6.25 US 25 50 100 Conc-% 10- s- '. 0 •5- •10- Report Date: 24 Jun-08 1 0:56 (p 2 of 2) Link/Link Code: 07-31 46-7449/0806-S058 Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes LOEL TOEL TU PMSD 50 35.4 4 21.6% Decislon(S%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Declslon(5%) Significant Effect Decislon(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 26 0.666 3.58 16.9% 0.0% 28 1 5.38 24.4% -4.25% 28 0.798 4.3 18.4% -10.4% 23 0.636 3.43 18.2% 11.3% 19 1.11 6 56.1% 49.5% 9 0.644 3.47 128.0% 87.3% ^^ S -2.5 -2.0 -1.5 -1.0 -0.5 0.0 OS 1.0 1.5 2.0 2.5 Ranklts 000-089-163-4 CETiS"»v1.6.3revE Analyst:QA: . CETIS Analytical Report Report Date: UnWUnk Coda: 24 Jun-08 10:56 (p 1 of 1) 07-3146-7449/0806-S058 Ceriodaphnia 7-d Survival and Analysis No: 20-3427-6000 Analyzed: 24 Jun-08 10:55 Linear Interpolation Options X Transform Y Transform Linear Linear Test Acceptability Attribute Control Resp Reproduction Test Endpolnt: Analysis: Seed 5334240 TestStat 21.2 Reproduction Linear Interpolation (ICPIN) Resamptes 280 Acceptability 15 -NL Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Exp96%CL Method Yes Limits Two-Point interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95V.LCL 95V.UCL 25 31.6 24 SO 48.7 41.6 Reproduction Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Reproduction Detail Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Graphics 25- ,^\ f: \ £ ID- S' 0 20 « 38.9 64.1 Calculated Varlate Count Mean 10 21.2 10 22.1 10 23.4 10 18.8 10 10.7 10 2.7 Rep 1 Rep 2 22 17 17 12 24 12 12 21 8 15 0 9 V 60 80 Caoc-V, Min 15 12 12 12 0 0 Rep 3 19 26 25 19 0 7 ^ 100 120 Max 26 28 28 23 19 9 Rep 4 15 25 22 21 8 0 StdErr 0.654 0.983 0.785 0.625 1.1 0.633 Rep 5 20 28 25 19 18 0 StdDev 3.58 5.38 4.3 3.43 6 3.47 Rep 6 21 28 25 16 19 5 CV% DhTK 16.9% 0.0% 24.4% -4.25% 18.4% -10.4% 18.2% 11.3% 56.1% 49.5% 128.0% 87.3% Rep 7 Rep 8 22 25 23 16 25 23 23 17 15 5 5 0 Rep 9 Rep 10 26 25 23 23 25 28 23 17 9 10 0 1 000-089-163-4 CETIS™ v1.6.3revE Analyst: CET1S Analytic.1 R.port Report Data: Unk/LInk Code: 24 Jurv-08 10:56 (p 1 of 2) 07-3146-7449/0806-S058 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 08-1890-8473 Endpoinfc Reproduction 24 Jun-08 10:55 Analysis: Parametric-Two Sample Data Transform Zeta Alt Hyp Monte Carlo NOEL Untransformed C>T Not Run <0 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 12.3% Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value DecIs<on(S%) Lab Control Conductivity Control 11.7 1.73 2.6 0.0000 Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp Control Resp PMSD ANOVA Table Source Between Error Total 21.2 3.6 0.123 15 -NL Yes 15 -NL Yes 0.13-0.47 Yes Passes acceptability criteria Eaila-Qoooptability criteria tf^-MA nt.svwnw/v'y (**££, Sum Squares Mean Square DP FStat P-Value Declslon(S%) 1548.8 1548.8 1 138 0.0000 202 11.22222 18 Significant Effect 1750.8 1560.022 19 ANOVA Assumptions Attribute Test Variances Distribution Variance Ratio F Shapiro-WUk Normality TestStat Critical P-Value Decision{1%) 1.34 6.54 0.6720 0.966 0.6680 Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95%UCL Mln 0 0 Graphics 30- ^ 20- f » ID- S' — Lab Control 10 21.2 19.8 22.6 15 Conductivity Co 10 3.6 2.42 4.78 0 0 (^(J^ Conc-% frn J • 18 • 2 -2- -«- -6- Rajnct Null n Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 26 0.666 3.58 16.9% 0.0% 9 0.575 3.1 86.1% 83.0% • \jf ^0 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 000-089-163-4 CETIS™ v1.6.3revE Analyst:.QA:. =reshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna mple ID: slumber: Cone. LC ^ Dudek-Carlsbad GC v4eii 3 Cra CAP -SC Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ CUD f? \ HI M«* i^3 uVu*5 HO 5fgT G&y\ &—• ^ Start Date/Time: 6/12/2008 155? £££ End Date/Time: 6/19/2008 (IMS Daily Reproduction/ Survival •;;*(l££! 0 0 0 0 O 0 9 36 0 «t_ :]:£ :?:;:•:• 6? 0 Oo A> A O 0oo E.i :::i:i:5&l:: 0 o O C> O Z-Ho 6> V £f i:::i:3$!:;;i: •V- 0 0 o 0 & U- *> fffe ;j:;i::j|ij:j:i' Ly (f la ^"7 -7 -j *^ffe d O ££_ SSiffiliisiiO •5 Oo 0 I2_ If lo 9 tot>uJ [ijjji'Sn::!:;: lH' vs (O A <l I1 ) ") , ,u^- IMF 9£> i|5!!l»n§!l Total e?-^* T^7 I ^is^od*t A3^ AS 3^§i^ QC 0 ^ Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 !{!jjj?!!j!;!!i:j{j li:iII=ii!|!;ilHii :•:•:•:•:•:•:•::::: :!:|i::|s|:!i!=:'!: il:!:||i|l|l|l|;|:| ... .^ ... b6 0ft& 0 0 0 0 & . 0 6 O oo 0 o o e>e Daily F t? <? o.6/4- (? C?O 0 oo (eprodu«:=:::= Oe? 0,^~ o d> 2> O oo ction/ S 4y 6 6 •— 0 f) «7 o O o jrvival •"•^S"1 €> O , — g ^y *j ,..,,„, S5o ~~~ U .•X O O O 3L ,.,,-.., — - Total f\ O_o o/d U- 3 T•A, H7 C0 Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 . . . . . ,J. . . 0 D fj 0 0o 0 0 0 • 2"" O5 ft ft O Oo 0 6 O Daily F. . . _ . . (9 0 O 0 O iry O O t> leprodu '£>" " 9tt A 9~ gag^ d-O p^ <#3 \(j9 3 o ^.3> Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 |:j:l!lj|iiji:!!!!i H!:-:::!:-:!:::!^ i':-:-:':-:::::':-': i:|;i:!:i:i:|:|:|:j i;;:i::rj;j::::;|]j i:i::::ji[!:::!jljl |:!:j:i:|jj:!iri!:: ::|:::|i|:|H:::::; Daily Reproduction/ Survival gij-iljijijiji O O U 0 d 0 0 0 0 O |j:::::3t:::i:: 6 0o 0 o 6 6 6 O O |.:il!:|S:i:Hi £7 O O O O O.30 0 Q i:i:.:ji$i:ijij \ d>o•3* ^^t) £> U "^S ij:ijji§:j::-: •4 10 p ^in 10 M d O IliiliS!!!!!! <{> °/fl 12. O 12. _Jtfi \2- -7 10 5? j:|i!:|3!:i:i|lb (M - \7f Mo C)o \3-\a. \*p ii:|!;®:IHi; - Total jm (l^idjfe^» ^^5&.V ^dSv2t5>as^•^ QC t") €S QC °IC-5 QC O £s me Fed/Test Solution Renewed (day): 4otes: d a dead; M ° male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included In total) omments: 1C Check:Final Review: Vautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek-Carlsbad GC Test Number: fl#Qj0 - Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pti/ex D. magna Start Date/Time: 6/12/2008 End Date/Time; 6/19/2008 UM5 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup H^ • H\ •TV u. O J^ J % . (I &l ST 5f i< nfl* Daily Reproduction/ Survival 0 0 0& 0 0 0 0 0 0 fty £;i!££|i e& 0 0 o 0 6 60 0 £S m$mc o•o 0 0o 2. 0 oo £p iiiili^liiii;c?ft 9- &•& g> psu 3- 0 <^t *^ ~7 *f $ g •-/ *7 O <5 V jfe"C ililHiSilliil O "^ftoo It 7 KZ- 4 <J ^3>PO Jiiiij^llllii 10 U «fc \\ 03. to Uv^. ^t& IHHllHil!!Total I3> 3*1 \^ ^ ^ _« ^MVD >.3 \T QC °\ E^ Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival•i • f> £6o # 0 0 0 (D 0 I ?... 0 6 6 ft d r-> 6 e 6 0 L. .?...!! 0o o/*L o o H 2. O Oo o &, — 0o G O O 0 0 illlills!!!!:! 3 5 —o "7 7 6 3jr V i!!|i!«!ii!ii _5o ~— oIt •5 •7 p O 0 !i|i:i|Sli!i|!ii O *g — ^O Q O 51 ^? (o iliijligijiii; - Total ^\S CYel ^!'?T i>£flb QC £.£<; Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 • Daily Reproduction/ Survival e 0 6 0 0 0 o 0 0 0 !H§!!*jJf PI ee 0 0 0 a 0o O mmmoo o 0 o O O Oo U mmmt» oo e> O 3~ & 3-z> & o \ mmm o 5 0<v^ O V*'o °/«f €) O illill^lHll Oo'f —o - 3— o o !!!)i!«lH — - - Total O ^-1 0/d CD s/dla>" O/oJoT QC 0€s Time Fed/Test Solution Renewed (day): jJJVj£_ (2i)n.s (3)Joi£ Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: QC Check:Final Review; Nautilus Environmental 5550 Morefiouse Drive, Suite f 50. San Diego, CA 92f 21 Freshwater Chronic Bioassay Client: Dudek-Carlsbad GC Sample ID: Test No: Water Quality Measurements Test Species: C. rft/b/a Start Date/Time: «maom End Date/Time: Conductivity Control Analysts: Initial: Final: Sample Used (A. B.C): Commonis* Animal Source/Date Received: A.G4: via Sample Log-In Numbers: A: QC Chock: <?& Animal Age at Initiation: B: Final Revlow: Nautilus Environmental 5550 Morehouse Drfve, Su«« ISO. San Drtgo, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client Dudek-Carisbad GC Test Species: C. dubia Sample ID: (jjg> U Test No:- 8(35% Start Date/Time: mma* End Date/Time:\Q.M Analysts: Initial: Final: Sample Used (A, B, C): Comments:. Animal Source/Date Received: PS- A MP ft Sample Log-In Numbers: A: QC Check: B: Animal Age at Initiation:. C: (33/08 Final Review: MiutUln Environment*! 5550 Mora/rouse Drive, Su/te 150. San D/ego, CA 32121. Site: Well 1 CETIS Summary Report Report Date: Link/Link Code: 24Jun-0811:28(p1 of 2) 21-2359-8147/0806-S059 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 06-4324-362£ Test Type: Reproduction-Survival (7d) Start Date: 12 Jun^o* 15:55 Protocol: EPA/821/R-02-013(2002) Ending Date J<^Un-08 08:50 Species: Ceriodaphnia dubia Duration: S"JL Ufl23^Tn Source: In-House Culture Sample No: 10-7023-4397 Code: 08-S059 Sample Date: 11 Jun-08 12:45 Material: Groundwater Receive Date: 12 Jun-08 10:10 Source: Dudek & Associates Sample Age: N/A (2.9 *C) Station: WelM Comparison Summary Analysis No Endpoint 1 7-4369-3130 7d Survival Rate 08-7862-9107 Reproduction (u^Owtro 14-5989-7064 Qjkbdftd w- TO Point Estimate Summary Analysis No Endpoint 12-0999-4628 Reproduction Test Acceptability Analysis No Endpoint 17-4369-3130 7d Survival Rate 08-7862-9107 Reproduction Reproduction 12-0999-4628 Reproduction 14-5989-7064 Reproduction 08-7862-9107 Reproduction 14-5989-7064 Reproduction NOEL LOEL 100 > 100 <0 0 50 100 Effect-% 25 50 Attribute Control Resp Control Resp Control Resp Control Resp Control Resp PMSD PMSD Conc-% 40.6 74.7 Test Slat 1 31.5 22.5 31.5 31.5 0.23 0.312 TOEL N/A N/A 70.7 Nautilus Environmental (CA> Analyst Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client: Dudek & Associates Project PMSD N/A 23.0% 31.2% Method Fisher Exact/Bonferroni-Holm Test Equal Variance t Two-Sample Test Wilcoxon/Bonferroni Adj Test 95% LCL 95% UCL Method 12.4 46.3 57.3 88.4 Linear Interpolation (ICPIN) Acceptably Limits Overlap 0.8 - NL 15 -NL 15 -NL 15 -NL 15 -NL 0.13-0.47 0.13-0.47 Yes Yes Yes Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 9 10 8 10 Mean 0.8 1 0.9 1 1 0.875 0.9 95% LCL 0.643 1 0.782 1 1 0.743 0.782 95% UCL 0.957 1 1 1 1 1 1 Min 0 1 0 1 1 0 0 Max 1 1 1 1 1 1 1 StdErr 0.077 0 0.0577 0 0 0.0645 0.0577 StdDev 0.422 0 0.316 0 0 0.354 0.316 CV% 52.7% 0.0% 35.1% 0.0% 0.0% 40.4% 35.1% Diff% 0.0% -25.0% -12.5% -25.0% -25.0% -9.37% -12.5% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 9 10 8 10 Mean 22.5 31.5 32.5 28.3 28.4 21.4 10.5 95% LCL 18.6 28.5 27.2 25.6 25.6 17.7 8.56 95% UCL 26.4 34.5 37.8 31.1 31.2 25 12.4 Min 6 18 0 19 14 0 2 Max 34 42 45 39 43 31 19 StdErr 1.91 1.48 2.59 1.34 1.38 1.78 0.948 Std Dev 10.5 8.09 14.2 7.37 7.56 9.77 5.19 CV% 46.6% 25.7% 43.7% 26.0% 26.6% 45.7% 49.4% Diff% 0.0% -40.0% -44.4% -25.9% -26.2% 5.0% 53.3% 000-089-163-4 CETIS™ v1.6.3revE CETIS Summary Report Report Date: Link/Link Code: 24Jun-0811:28(p2of 2) 21 -2359-8147/0806-S059 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rept 1 1 1 1 1 1 1 Rep 2 1 1 1 1 1 0 Rep 3 1 1 1 1 1 1 1 Rep 4 1 1 1 1 1 1 1 RepS 1 1 1 1 1 1 Rep 6 0 1 0 1 1 1 Rep 7 0 1 1 1 1 1 1 RepS 1 1 1 1 1 0 1 Rep 9 1 1 1 1 1 1 1 Rep 10 1 1 1 1 1 1 1 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 26 34 31 19 34 23 6 Rep 2 33 25 33 28 22 2 Rep 3 34 34 44 30 27 16 12 Rep 4 31 36 37 39 43 29 13 RepS 22 18 41 38 29 12 Rep 6 6 42 0 28 14 17 Rep 7 6 37 36 20 29 31 19 RepS 24 30 16 21 32 0 6 RepS 14 39 45 32 22 24 9 Rep 10 29 20 42 28 26 26 9 000-089-163-4 CETIS™ v1.6.3revE Analyst! CETIS Analytical Report Report Date: LJnk/Unk Code: 24 Jun-08 11:28 (p 1 of 1) 21-2358-8147/0806-8059 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 17-4369-3130 Endpoint 24 Jun-08 11:27 Analysis: 7d Survival Rate CETIS Version: CETISvl .6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zata Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C> T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Teat Control vs Conc-% TestStat P-Value Dectslon(O.OS) Lab Control 6.25 0.5 12.5 1 25 1 50 0.444 100 0.5 Test Acceptability Attribute TestStat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- a* «j 0> •1 0.6- « Q.S- 03-, 0.1-. 0.0 Lab Control 10 0 9 1 9 0 10 0 7 1 9 1 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 -NL Yes Passes acceptability criteria Total 10 10 9 10 8 10 • e • e A e 0 6.25 IIS 2S Conc-% 50 100 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 24Jun-0811:28(p1of 1) 21-2359-8147/0806-S059 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 12-0999-4628 Endpoink Reproduction 24 Jun-08 1 1 :28 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Resamples Linear Linear 5795186 280 Nautilus Environmental (CA) CETIS Version: CETlSvl .6.3 Official Results: Yes Exp 96% CL Method Yes Test Acceptability Attribute TestStat Acceptability Limits Control Resp 31.5 15 -NL Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95%LCL 95% UCL 25 40.6 12.4 57.3 50 74.7 46.3 88.4 Reproduction Summary Conc-% 0 6.25 12.5 25 50 100 Control Type Count Lab Control 10 10 9 10 8 10 Mean 31.5 32.5 28.3 28.4 21.4 10.5 Win 18 0 19 14 0 2 Calculated Variate Max 42 45 39 43 31 19 StdErr 1 2 1 1 1 .48 .59 .34 .38 .78 0.948 StdDev 8.09 14.2 7.37 7.56 9.77 5.19 CV% 25.7% 43.7% 26.0% 26.6% 45.7% 49.4% Dlff% 0.0% -3.17% 10.1% 9.84% 32.1% 66.7% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 35- 1 30- 25- § 1 20- 1 15- 10- S- Control Type Rep 1 Lab Control 34 31 19 34 23 6 \ X.N.^\^ 0 20 40 60 Conc-% Rep 2 25 33 30 28 22 2 \ to Rep 3 34 44 39 27 16 12 ^» 100 120 Rep 4 36 37 38 43 29 13 Rep 5 18 41 28 29 31 12 Rep 6 42 0 20 14 0 17 Rep 7 37 36 21 29 24 19 Rep 8 Rep 9 30 39 16 45 32 28 32 22 26 6 9 Rep 10 20 42 26 9 000-089-163-4 CETIS ™v1.6.3revE Analyst; CETIS Analytical Report Report Data: Link/Link Coda: 24Jun-0811:28(p2of 3} 21-2339-8147/0806-S059 Cerlodaphnia 7-d Survival and Reproduction Test Analysis No: 14-5989-7064 Endpoinfc Reproduction Analyzed: 24 Jun-08 1 1 :27 Analysis: Nonparametric-Multlpte Comparison Data Transform Rank Wilcoxon/Bonferron! Adj Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100* Test Acceptability Attribute Control Resp PMSD Zata Art Hyp OT Monte Carlo Not Run TestStat Critical 116 79.5 91 52 56 TestStat Acceptability Ties 3 3 1 0 0 Limits 31.5 15 -NL 0.312 0.13-0.47 ANOVA Table Source Sum Squares Mean Square DP Between 3392.436 Error 4261.775 Total 7654.21 ANOVA Assumptions Attribute Test 678.4871 83.56422 762.0513 Variances Barttett Equality of Variance Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Rank Transformed Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Count 10 10 9 10 8 10 Count 10 10 9 10 8 10 Mean 31.5 32.5 28.3 28.4 21.4 10.5 Mean 37.7 40.4 32.2 32.1 22.4 8.25 5 51 56 Test Stat 9.95 0.927 95% LCL 28.4 27.1 25.5 25.5 17.7 8.53 95% LCL 32.5 33.2 27.4 27.5 18.1 6.62 FStat 8.12 Critical 15.1 95% UCL 34.6 37.9 31.1 31.3 25.1 12.5 95% UCL 42.9 47.6 37 36.6 26.7 9.88 NOEL 50 P-Value 1.0000 0.8000 0.6300 0.0686 0.0000 Overlap Yes Yes P-Value 0.0000 Nautilus Environmental (CA) CETIS Version: CETISvl Official Results: Yes LOEL 100 TOEL 70.7 TU 2 .6.3 PMSD 31.2% Declslon(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Decision(6%) Significant Effect P-Value Decision(1%) 0.0765 0.0021 Mln 18 0 19 14 0 2 Min 15 1.5 16.5 11 1.5 3 Equal Variances Non-normal Distribution Max 42 45 39 43 31 19 Max 53.5 57 50.5 55 37.5 16.5 StdErr 1.5 2.64 1.37 1.4 1.81 0.964 Std Err 2.53 3.5 2.34 2.24 2.1 0.798 StdDev 8.09 14.2 7.37 7.56 9.77 5.19 StdDev 13.6 18.9 12.6 12.1 11.3 4.3 CV% 25.7% 43.7% 26.0% 26.6% 45.7% 49.4% CV% 36.2% 46.7% 39.2% 37.6% 50.4% 52.1% Diff% 0.0% -3.17% 10.1% 9.84% 32.1% 66.7% Diff% 0.0% -7.16% 14.7% 15.0% 40.5% 78.1% 000-089-163-4 CETIS™ v1.6.3revE CETIS Analytical Report Report Date: Link/Link Code: 24Jun-0811:28(p3of 3} 21-2359-8147/0806-8059 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 14-5989-7064 Analyzed: 24 Jun-0811:27 Endpoint Reproduction Analysis: Nonparametric-Muttiple Comparison CETIS Version: CETISv1.6.3 Official Results: Yes Graphics 5<h 40- 30- 20- RejictNill 6.25 12J5 25 Conc-Vo 50 100 -2.S -2.0 -1.5 -1.0 -0.5 0.0 O.S 1.0 1.5 2.0 2.5 000-089-163-4 CETIS™ v1.6.3revE Analytical Report Report Date: Link/Link Code: 24Jim-0811:28(p1of 3) 21-2359-8147/0806-S059 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 08-7862-9107 Endpolnt: 24 Jun-08 1 1 :28 Analysis: Reproduction Parametric-Two Sample Data Transform Zeta Alt Hyp Monte Carlo NOEL Untranstormed C > T Not Run <0 Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Lab Control Conductivity Control 2.15 Test Acceptability Attribute Test Stat Control Resp Control Resp PMSD ANOVA Table Source Between Error Total 31.5 22.5 0.23 1.73 7.26 0.0227 Acceptability Limits Overlap 15 -NL Yes 15 -NL Yes 0.13-0.47 Yes Sum Squares Mean Square DF F Stat P-Value 405 405 1577 87.61111 1982 492.6111 ANOVA Assumptions Attribute Test Variances Distribution Variance Ratio F Shapiro-Wilk Normality 1 4.62 0.0454 18 19 TestStat Critical P-Value 1.68 6.54 0.4520 0.907 0.0570 Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Min 0 0 Graphics so- 40- 1 30J I 20- 10- Lab Control 10 31.5 Conductivity Co 10 22.5 0 (^C, Cone-% 28.4 34.6 18 18.5 26.5 6 15-; ID- *! -• !-J• » „a§ o.Of : a * Reject Nu8 -10- -15- Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 23.0% Decteion<5%) Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Declslon(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% Diff% 42 1.5 8.09 25.7% 0.0% 34 1.95 10.5 46.6% 28.6% y\ • *T '±*/'~ ./+ /• -/ • / • / * 0 -2.0 -IS -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 LC R*nWt» 000-089-163-4 CETIS™ v1.6.3revE Analyst. Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D.pulex D. magna Client/Sample ID: Test Number: Cone. LC ;-i . •'.-:'•:•:'•:• .--•':'.':-' •'.'•'.' ''"''•'': ' ', Dudek-Carlsbad GC l/\Wl (Start Date/Time: 6/12/2008 l/SS bSTOVe-S1^*] End Date/Time: 6/1072008 Qft^O Rep 1 2 3 4 5 6 7 f 9 10 Analyst Verified by: Board/ Cup ij i £ J 1 9 1 PL •^ M •5 £ 9> ( 3Ct^- tf/U A^H! Cone. CC :i£!:£|i;i;i!i|iN Rep 1 2 3 4 5 6 7 8 9 10 • Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival •:::-::Hji:;j:: b b 0o o 0o 0 o 0 Js*S M:!:!*|:!:|: 0 c> t> 0 0o o *c> /5^ O £-.& i:j:ii^:i:j:: Oi. oooo Q T Q0 jftf :l:i:ISi:i:;: 0 O & & 'V-oc? CPe>c<? ^ :;:;::S::::-:i ',1' "ID A i^- 0 *i a a R A TA Hi*!*; (1 I3> 10 8&, t> o t-7 O f) OV\/ :=;;:;:?;:•:•:• 0oo It*o 75ovp. O \5\\j> £0" W-:®-:;:;: 'ffc/JttFrt<j '4'£•c^ %1$ yj ^ts Daily Reproduction/ Survival _ . |£::|:[:i <9 O O O o ooo f o !j!:-|J3|!:;:i| p .^f9o o C?t? 0 0 O <3o 0 O 0 0 0uou 4 ;:|;i|"%/) cL^. o ?~k *, 9o Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 ii:j:!:3:i:iii'tr&•^A~y ^rt/^t> «H ;:!ii!»:lli!l 0 /o 0 O 1 O/df Orfrf *3 U1 0 Jiiliil?;^;i!<ao \v 14 J«Y^> ^ &*A&l<fM~* ju ^.& 1 *^, n iljiiijSljijlji - j^js MA ^IT 6^ —_»- O O\u- Z* Total i 3ii 'T 1 ) 15 : v 3H =y 5U • t* ' U.-2-> ^"^» / 1W)~v( 1.0 QC Total 2.U ?^'3fV- 21?if \ra & & I £\ 2L . )ii • 5L*l QC J3 Daily Reproduction/ Survival •:!(•:•:•:• 0 0 O oO e>c> o 0o :i:i:|:»::i:i #$=r {") 0robn6 Vft hi::::*::;:: 0 oooo/A. 0 "2«i P-\5 I:-::::!*;:::;: ff 0(.(.? > o_ 5o oo ;:|S|:|$J:::!: T t\ t ft 10 (•£•— !:!!!*!;!!!:•o 1-7 i£ o jg_ —134 c? O ::::::!p:-:::i:|ip^ 6 \d* O__ 0 li^\M t?> :;j;;;i8Jj;;:;: \3t |4; fe ^ B\°iid* — \4 ^t i o Uto fi.r Total 3\ 3f> u-iV ^ -f !• | o\o\. "2h>\u ^?M-2- QC \"i-^ Daily Reproduction/ Survival 1 O fto oo 0 0 ao o 2c)n uoo 2ft& o '? •2. 5 O 0 0 0 2. ¥ O0 4=:;:::o O o 1. i!:!j::5--:=:; Ib —v^1 \Aa (a 7 (p t(J ;::::::|J:;.::: •7 —C/ "^LOr.i (2. £<3 il /e> :•:=:!:?:::':=: Q — O \^ts- Oo 0 \f^ o j:j:j:ifii-::& \W9ffi_ _ \ TUi ^ 3n /- \S.Vri5cicb /P IM>E Total ) \*^ 6'juAf ' &O .21 ^A 2.DI 5-o i 2.1 X2L £/" QC 13 b Time Fed/Test Solution Renewed (day): ou&^L o *Mte (2) _G^2_ (3) Notes: d = dead; M = male; LIP = lost In progress; B a 4th brood (only the first 3 broods are included in total) omments: QC Check:Final Review: Nautilus Environmental 5550 More/louse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bloassay Client/Sample ID: Dudek-Carlsbad GC Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 6/1272008 1 565 End Date/Time: 6/^2008 Cone.Rep Board/ Cup m Dally Reproduction/ Survivalin Total QC 25%0 \\o o o &\s>6 o f)o 6 15 oo £& 6 0 10 O HAnalyst Verified by: £.<. Cone.Rep Daily Reproduction/ Survival Total QC 50% o 10 0 ID ou 4iL0Uvo& \(n 10 n Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 •Daily Reproduction/ Survival ll&Hii 6 o 0 o O 0 0 o 0 O O r>r?r> OO 0 cO 0 ft o 2-0oo 0 0 0 0o !t;!*$ji £ O H M 55 3 0 0 o ;^ illlJifciii!!! O O 0 -0£> 00 0o d i=i=;i«ii!!i! O 0/^/ d? 0 0 o •7 O o O liiiii^iilHi 3 — ^1-4 \M 0 O 1 Lo iiiiiiiSiiiii: o- oooo \-3t La O O Total U ^/dii \^ \2_ »"t t°l ^£1q QC 1 Time Fed/Test Solution Renewed (day): (0) j^??/ MlniS | Notes: d 3 dead; M s mate; LIP »lost in progress; B » 4th brood (only the first 3 broods are included in total) Comments: QC Check:Final Review: Nautilus environmental 5550 Monhouse Drive, Suite 150. San Diego. CA 92121. Freshwater Chronic Bioassay Client: Dudek-Carlsbad GC Sample ID: Test No: DSD U Water Quality Measurements Test Species: C. dubla Start Date/Time: tmaan End Conductivity Control Initial: Final: Sample Used (A, B, C): .y-^ ,t<,«f>e JAnimal Source/Date Received: ItH'TC-fV^^i / A/A /*) ii ^_n| I Sample Log-in Numbers.: A: QC Check: Animal Age at Initiation: L 3 Final Review: Q>£ ClfeUfflff -v Nautilus Environmental 5550 More/loose Drive, Suite 150. San Diego, CA 92131. Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek-Carlsbad GC Sample ID: _ Test No: Test Species: C. dubia Start Date/Time: tmom End 08*70 Analysts: Initial: Final: Sample Us«d( A, B,C): ^U~ PP Animal Source/Date Received: Sample Log-In Nqmbefs: Al QC Check: Animal Age at Initiation: C: +0 °'VV' NfUtBin Emlmmutttt 5550 Monhoun Drtn, Suite 150. S*n Dbgo, CA 93121. Appendix B Sample Receipt Information Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Client: Sample ID: Tests Performed: ££,_ Sample (A, B,C) Log-In No. (08-xxxx) Sample Collection Date & Time Sample Receipt Date & Time Check-in Temperature (°C) Temperature OK? DO (mg/L) pH (units) Conductivity (uS/cm) Salinity (ppt) Alkalinity (mg/L)* Hardness (mg/L)*1 Total Chlorine (mg/L) Technician Initials -7-82 0.62- N N N 5.H \-c, tt * = mg/L as CaCO3, * <* Measured for freshwater samples only, NA » Not Applicable /Freshwater Tests: i Control/Dilution Water Source: Additional Control? Marine Tests; Control/Dilution Water Source: LABSW ARTSW Additional Control? Y N = Culligan N = Other.Alkalinity: JO I .Alkalinity: Other:Alkalinity: Alkalinity:. Hardness: Hardness: Salinity:. Salinity:. NSample Salted w/ artificial salt? Y Sample salted w/bn'ne? Y N If yes. what ppt?. If yes, what ppt?. Sample Check-In Information Sample Description: ftu COC Complete? A B C_ Filtration? Pore Size: Organisms or Debris Aeration? Y {fiT) Length of Time: Final DO: Final pH:s*\pH Adjustment? Y Initial pH: Final pH: CI2 Adjustment?~^rre^— B^'KtA Initial Free Cl,: STS added: Final Free CI2: Sub-samples for additional chemistry: Comments: Temperature for sample must be 0-6'C if received >2A hours past collection time. QC Check: Of\ Final Review: Glossary of Qualifier Codes: Q1 - Wrong Entry (misspelled, copy error, wrote in wrong spot, etc) Q2 - Temps out of recommended range, refer to CAR Q3 - DO out of recommended range, refer to CAR Q4 - Salinity out of recommended range, refer to CAR Q5 - Miscalculation Q6 - Spilled test chamber/ Lost test animal Q7- Instrumentation Error/Failure Q8 - Inadequate sample volume, 50% renewal Q9 - Inadequate sample volume, no renewal performed Q10 - Sample out of holding time Q11 - Refer to QA section of report for explanation Q12 - Supplemental information is footnoted Q13 - Test initiated with an incorrect number of test organisms Q14 - Replicate(s) not initiated Q15 - Survival counts not recorded due to poor visibility or heavy debris Q16 - Test placed on air due to DO levels dropping below 4.0 mg/L Q17 - Test initiated on air due to an anticipated drop in DO Updated 5/12/08 Appendix C Chain-of-Custody Form I CALIFORNIA D WASHINGTON D BRITISH COLUMBIA 5550MowtaiaDii«.Stitel50 50)9 (toft Kghwoy East-Site 2 8664 Comrnra Court SonKeQo,Cdfcn>Q92)21 komo,WnstingtD(i98424 Bwnoby BdtishColumbia CtmodaV5A4N7 Phone 858587.7333 Pta» 253.922.4296 fhme604.420.8773 Fm858.587.3961 Fax 253.922.5814 Fax604.3S7.1361 Date Chain of Custody ANALYSES REQUIRED Phone/Email SAMPtf ID DATE TIME MATRIX Invoice to: Company Address State -Zip- Contact Pnone/Emoil . CONTAINER WE NUMBER OFOONWNHS COMMENTS Wll mnv V- —|rad fof—-Je dbop"1 ~, storae- KM^O unless oriwrwite conlwyed.DISTRIBUTION: WHITE - Nautilus Environmental, COLOR • Originator October 6, 2008 EnviroMatrix I M I Analytical, Inc. 06 October 2008 Dudek & Associates, Inc. EMA Log #: 0809564 Atta: Chris Oesch 605 Third Street Encinitas, CA 92024 Project Name: Carlsbad Golf Course Enclosed are the results of analyses for samples received by the laboratory on 09/26/08 12:57. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. I certify that this data is in compliance both technically and for completeness. Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewridge Avenue, Suite A • San Diego, California 92123 • (858) 560-7717 • Fax (858) 560-7763 Analytical Chemistry Laboratory Client Name: Dudek & Associates, Inc. EMALogtf: 0809564 Project Name: Carlsbad Golf Course ANALYTICAL REPORT FOR SAMPLES I Sample IP ^ Laboratory ID Matrix Date Sampled Date Received Downstream 0809564-01 Water 09/25/0809:50 09/2670812:57 Upstream 0809564-02 Water 09/25/0811:00 09/26/0812:57 Well 3 0809564-03 Wat« 09/25/0812:00 09/26/0812:57 Welll 0809564-04 Water 09/25/0815:30 09/26/0812:57 The results in this report apply to ike samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. Page 2 of7 - BatriroMatriz I ~M 1 Analytical, Inc. Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EM A Log #:0809564 Conventional Chemistry Parameters by Standard/EPA Methods Analyte Result Reporting Limit Units Dilution Batch Prepared Analyzed Method Notes Downstream (0809564-01) Water Sampled: 09/25/08 09:50 Received: 09/26/08 12:57 Nitrate as N Nitrite as N Dissolved Oxygen pH Phosphorus, Total Total Suspended Solids 0.43 ND 3.47 7.84 0.12 29 Upstream (0809564-02) Water Sampled: 09/25/08 11 Nitrate as N Nitrite as N Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids Well 3 (0809564-03) Water Sampled: Nitrate as N Nitrite as N Dissolved Oxygen pH Phosphorus, Total Total Suspended Solids Well 1(0809564-04) Water Sampled: Nitrate as N Nitrite as N Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids 0.45 ND 3.13 7.65 0.06 ND 09/25/08 12:00 31.9 ND US 6.70 0.26 112 09/25/08 15:30 ND ND 1.03 5.85 0.42 235 0.05 0.05 0.10 0.10 0.05 20 mg/1 « n pH Units mg/1 n :00 Received: 09/26708 0.05 0.05 0.10 0.10 0.05 20 Received: 2.50 0.05 0.10 0.10 0.05 20 Received: 0.05 0.05 0.10 0.10 0.05 20 mg/1 n ir pH Units mg/1 n 09/26/08 12: mg/1 N It pH Units mg/1 n 09/26/08 12: mg/1 W n pH Units mg/1 ir 1 IT * tt n n 12:57 1 n N M " 11 57 50 1 » " n tt 57 1H n n " " 8100230 8092902 8100220 8093001 8100316 8092925 8100230 8092902 8100220 8093001 8100316 8092925 8100230 8092902 8100220 8093001 8100316 8092925 8100230 8092902 8100220 8093001 8100316 8092925 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/29/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/29/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/29/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/29/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/30/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/30/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/30/08 10/02/08 09/26/08 09/26/08 09/29/08 10/03/08 09/30/08 SM4500 NO3 E SM4500 NO2 B SM4500-OG SM4500-H+ B SM4500PB.E SM2540D SM4500NO3E SM4500NO2B SM4500-O G SM4500-H+ B SM4500PB.E SM2540D SM4500NO3E SM4500NO2B SM4500-O G SM4500-H+B SM4500PB.E SM2540 D SM4500NO3E SM4500 NO2 B SM4500-0 G SM4500-H+ B SM4500 P B, E SM2540 D W-02 HT-15 HT-04 W-02 HT-15 HT-04 W-02 HT-15 HT-04 W-02 HT-15 HT-04 TTie results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. BmriroHatrix Analytical. Inc. Page 3 of 7 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course Conventional Chemistry Analyte Result EM A Log #: 0809564 Parameters by Standard/EPA Methods - Quality Control Reporting Limit Units Spike Source %REC RPD Level Result %REC Limits RPD Limit Notes Batch 8092902 Blank (8092902-BLK1) Nitrite as N ND LCS (8092902-BS1) Nitrite as N 0.10 LCS Dap (8092902-BSD1) Nitrite as N 0.10 Duplicate (8092902-DUP1) Nitrite as N ND Matrix Spike (8092902-MS1) Nitrite as N 0.08 Matrix Spike Dap (8092902-MSD1) Nitrite as N 0.08 Batch 8092925 Blank (8092925-BLK1) Total Suspended Solids ND Duplicate (8092925-DUP1) Total Suspended Solids 48.0 Reference (8092925-SRM1) 0.05 mg/1 0.05 mg/1 0.05 mg/1 Source: 0809564-04 0.05 mg/l Source: 0809564-04 0.05 mg/1 Source: 0809564-04 0.05 mg/1 20 mg/1 Source: 0809550-01 20 mg/1 Prepared & Analyzed: 09/29/08 Prepared & Analyzed: 09/29/08 0.100 102 80-120 Prepared & Analyzed: 09/29/08 0.100 105 80-120 3 20 Prepared & Analyzed: 09/29/08 ND 20 Prepared & Analyzed: 09/29/08 0.100 ND 82 80-120 Prepared & Analyzed: 09/29/08 0.100 ND 85 80-120 4 20 Prepared: 09/29/08 Analyzed: 09/30/08 Prepared: 09/29/08 Analyzed: 09/30/08 49.0 2 20 Prepared: 09/29/08 Analyzed: 09/30/08 Total Suspended Solids 54.5 20 mg/1 65.3 83 1.93-106.5 The results in (His report apply to the samples analyzed In accordance with the chain of custody document. This analytical report must be reproduced in its entirety. BnTiroMvtrix [Ml Analytical, Inc. • Page 4 of7 Client Name: Dudck & Associates, Inc. Project Name: Carlsbad Golf Course Conventional Chemistry Analyte Result EMALogft 0809564 Parameters by Standard/EPA Methods - Quality Control Reporting Spike Source %REC RPD Limit Units Level Result %REC Limits RPD Limit Notes Batch 8093001 Duplicate (8093001-DUP1) pH 7.83 Reference (809300 1-SRM1) pH 9.03 Batch 8100220 Duplicate (8100220-DUP1) Dissolved Oxygen 3.46 Batch 8100230 Blank (8100230-BLK1) Nitrate as N ND LCS (8100230-BS1) Nitrate as N 0.48 LCS Dup (8100230-BSD1) Nitrate as N 0.50 Duplicate (8100230-DUP1) Nitrate as N ND Matrix Spike (8100230-MS1) Nitrate as N 0.50 Source: 0809564-01 Prepared & Analyzed: 0.10 pH Units 7.84 Prepared & Analyzed 0.10 pH Units 9.10 09/29/08 02 20 HT-1S 09/29/08 99 97-102.96 Source: 0809564-01 Prepared & Analyzed: 09/26/08 0.10 mg/1 3.47 Prepared & Analyzed: 0.05 mg/1 Prepared & Analyzed: 0.05 mg/1 0.500 Prepared & Analyzed: 0.05 mg/1 0.500 Source: 0809563-08 Prepared & Analyzed: 0.05 mg/1 ND Source: 0809563-08 Prepared & Analyzed: 0.05 mg/1 0.500 ND 0.3 20 HT-15 10/02/08 10/02/08 97 80-120 10/02/08 101 80-120 4 20 10/02/08 20 10/02/08 101 80-120 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix [Ml Analytical, Inc. Page 5 of7 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EMALogtf: 0809564 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Reporting Result Limit Units Spike Source %REC RPD Level Result %REC Limits RPD Limit Notes Batch 8100230 Matrix Spike Dup (8100230-MSD1) Nitrate as N Reference (8100230-SRM1) Nitrate as N Batch 8100316 Blank (8100316-BLK1) Phosphorus, Total LCS (8100316-BS1) Phosphorus, Total LCS Dup (8100316-BSD1) Phosphorus, Total Duplicate (8100316-DUP1) Phosphorus, Total Matrix Spike (8100316-MS1) Phosphorus, Total Matrix Spike Dup (8100316-MSD1) Phosphorus, Total Source: 0809563-08 0.52 0.05 0.63 0.05 ND 0.05 0.49 0.05 mg/1 mg/1 mg/1 mg/1 0.50 0.05 mg/1 Source: 0809564-01 0.12 0.05 mg/1 Source: 0809564-01 0.61 0.05 mg/1 Source: 0809564-01 0.74 0.05 tag/I Prepared & Analyzed: 10/02/08 0.500 ND 103 80-120 2 20 Prepared & Analyzed: 10/02/08 0.688 91 W.12-107.: Prepared & Analyzed: 10/03/08 Prepared & Analyzed: 10/03/08 0.500 97 80-120 Prepared & Analyzed: 10/03/08 0.500 100 80-120 3 20 Prepared & Analyzed: 10/03/08 0.12 0.8 20 Prepared & Analyzed: 10/03/08 0.500 0.12 98 80-120 Prepared & Analyzed: 10/03/08 0.500 0.12 122 80-120 18 20 QM-05 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. Entr Ktrii M. 1 Analytical. la Page 6 of7 Client Name: Dudek & Associates, Inc. EMALog#: 0809564 Project Name: Carlsbad Golf Course Notes and Definitions W-02 The sample for nitrate analysis was preserved with H2SO4 after the nitrite portion of the analysis was completed to extend the holding time for the sample. Nitrate results are corrected for the nitrite contribution per the method. QM-05 The spike recovery was outside acceptance limits for the MS and/or MSD due to matrix interference. The LCS and/or LCSD were within acceptance limits showing mat the laboratory is in control and the data is acceptable. HT-15 This sample was received outside of the EP A's recommended 15 minute holding time for this analysis. However, the sample was analyzed immediately upon receipt HT-04 This sample was received outside of the EPA recommended holding time for this analysis. ND Analyte NOT DETECTED at or above the reporting limit NR Not Reported dry Sample results reported on a dry weight basis RPD Relative Percent Difference The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. Page 7 of7 - EnviroMatriz [ ~M 1 Analytical, Inc. CHAIN-OF-CUSTODY FORM page of_Analytical, Inc. Avt. Ste. A • San Diego, CA 92U3 • Phone (SSI) 561-7717 • FM IK*) 5*0-7763 GO? ?P* sr Sample Date/Time Coattineiti) # of/Type Saauk Matrix Coder. OW°graiind water WW-waste water DW-drinlting water SW-storm water A-«ir SED-wdimuit O-oil T=tisiue S-tolid other (specify) LxUquid other (tpeeity)RBLINQUISHEDBy Skipped By: llCmmer flUPS UFcdBx nUSPS ClClientdtopofr QCHbcr TurntroonilTUM: n&meifay 1124 hr IMShr naday H4day Hi (fay JgTPP day) •ReporUn,Rmiilr.inentt: UFax 1IPDF clExcel llGeotnckerEOF nOtbcr Sample DUpail: I !By Laboratory 11'Rclurn lo client P/U or Delivery r I Archive until Correct Coatamen: Yei No N/A Custody Seals Intact: Yes No N/A arapledBy: OCIient LIEMA OCOOLabob Agree: Yu No N/A White-EMA Canary - Accounting Pink- Client (w/Repon)Ooldentod - Client (Relinqulah Samples)'Addilioosl com may apply, consult a project manager for details. 'EMA reserves the right to return any samples that do not match our waste profile. Note: By relinquishing samples la EnviroMstrix Analytical, Inc., client agrees to pay for the services requested on this chain of custody form and any additional analyses performed on this project. Payment for services is due within 10 days rrom the date of the invoke. Ssmple(s) will be disposed of alter 30 days unless otherwise noted All work is subject to EMA's terras and conditions. CxH.invtw.HifcK.tAl California 5550 Morehouse Drive •Suite 150 Son Diego, CA 92121 858-587-7333 fax:858-587-6769 Washington 5009 Pacific Highway East Suite 2 •TacomaWA 98424 253-9224296 fax:253-922-5814 British Columbia 8664 Commerce Court Bumaby. British Columbia V5A4N7 604-603-9381 fax:604-603-9381 Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Testing Period: September 2008 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 October 30, 2008 Data Quality Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified Bv:yfjfcc^ ^LiA^-. Date: 10-I*-** TOXICITY SUMMARY REPORT Test ID Number: 0809-S166, 67,68 and 69 Client: Dudek & Associates Monitoring Period: September 2008 Sample Information Client: Dudek & Associates, Inc. Project Study: Carlsbad Golf Course Toxicity Evaluation - September 2008 Sample Site ID's: a) Downstream b) Upstream c) Well #3 d)Well#1 Sample Collection Date, Time: 9/25/08,10:40 to 15:30 Sample Receipt Date, Time: 9/26/08, 09:55 Sample Material: Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Control: Protocol Used: Test Acceptability Criteria: 9/26/08 to 10/3/08 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, < 24 hours 100, 50, 25, and 12.5%; plus Lab and Conductivity Controls *The 6.25% was not tested due insufficient amount of animals. Lab freshwater (diluted mineral water); conductivity = 200 uS/cm Lab freshwater spiked with seawater to raise the conductivity to match the highest sample conductivity, which was approximately 5,000 uS/cm. This control was conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Table 1. Water Quality Information: Samples Upon Receipt Sample ID Downstream Upstream Well #3 Well #1 pH 7.82 7.72 6.91 6.34 DO (mg/L) 10.6 10.0 7.3 7.8 Temp <°C) 6.0 6.0 6.0 6.0 Conductivity 1 ((iS/cm) 3210 3340 4450 3020 Hardness (mg/L as CaCOj) 996 1086 1525 860 Alkalinity (mg/L as CaCO3) 234 178 230 59 1 The conductivity meter was not measuring accurately at the time of sample receipt (temperature adjustment factor not working). Actual conductivity levels of each sample were measuring approximately 500 uS/cm higher at test initiation. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Test ID Number: 0809-S166,67,68 and 69 Client: Dudek & Associates Monitoring Period: September 2008 Table 2. Summary of Test Results Sample ID Concentration (%) Lab Control Downstream 12.5 25 50 100 Lab Control Upstream 12.5 25 50 100 Lab Control Well #3 12.5 25 50 100 Lab Control Well#1 12.5 25 50 100 Conductivity 100 Control Mean Percent Survival 100 90 90 100 100 100 80 100 90 90 100 100 100 80 40* 100 100 100 90 100 80 Mean Number of Offspring Produced 20.4 24.8 20.9 23.1 16.5 20.0 18.7 24.0 21.6 11.9* 21.8 14.6* 13.2* 7.0* 0.4* 19.9 13.7 12.9* 13.9 6.6* 3.3* * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Note: The Conductivity Control was shared with all of the sample sites. However, the Conductivity Control matched the sample with the highest conductivity, which was the Well #3 sample with a conductivity level of approximately 5,000 uS/cm. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: 0809-S166,67,68 and 69 Monitoring Period: September 2008 Table 3. Summary of Statistical Results and Reportable Values Sample ID Test & Endpoint Downstream: Survival Reproduction Upstream: Survival Reproduction Well #3: Survival Reproduction Well#1: Survival Reproduction NOEC (% effluent) 100 100 100 50 50 <12.5 100 50 EC*, (% effluent) >100 >100 >100 >100 84.1 34.3 >100 74.0 TUC (toxicity units) 1.0 1.0 1.0 2.0 2.0 >8.0 1.0 2.0 NOEC = the highest Concentration that produces No Observed Effect ECso = concentration that causes a 50% adverse effect to the test organisms Chronic Toxicity Units (TUc) = 1004 NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. There was no toxicity observed to the survival endpoint with three of the sample sites. However, the Well #3 sample did show toxicity to survival at the 100% concentration. More of an effect was observed with the reproduction endpoint The Downstream sample showed no reproduction toxicity, but both the Upstream and Well #1 samples did show toxicity at the 100% concentration. The Well #3 sample resulted in toxicity at every concentration, down to the 12.5% concentration. The adverse effects to reproduction were expected however, due to the elevated levels of conductivity found in the samples. Through internal studies and experience, we have consistently observed adverse effects to the reproduction endpoint when conductivity levels exceed approximately 2,500 uS/cm. Conductivity is related to the concentration of salts in a sample. The optimum range for Ceriodaphnia is between approximately 100 and 1,500 uS/cm. The correlation between higher conductivity levels and decreased reproduction in Ceriodaphnia was observed in this study as well. Since all four samples had conductivity levels greater than 2,500 uS/cm (ranging from 3,400 to 5,200 uS/cm), an adverse effect to the reproduction was expected. The Well #3 sample had the highest conductivity level (approximately 5,000 uS/cm), and as expected, showed the greatest decrease in the number of offspring produced. The 100% concentration of Well #3 resulted in a 98% decrease in reproduction compared to the Lab Control. The Conductivity Control resulted in an 85% decrease in reproduction, but showed no toxicity with the survival endpoint. The slight increase in toxicity found in the Well #3 sample, compared to the Conductivity Control, may indicate that another factor might have contributed to the toxicity, in addition to the high conductivity levels. Nautilus Environmental - San Diego Bioassay Laboratory Glossary of Qualifier Codes: Q1 - Wrong Entry (misspelled, copy error, wrote in wrong spot, etc) Q2 - Temps out of recommended range, refer to CAR Q3 - DO out of recommended range, refer to CAR Q4 - Salinity out of recommended range, refer to CAR Q5 - Miscalculation Q6 - Spilled test chamber/ Lost test animal Q7- Instrumentation Error/Failure Q8 - Inadequate sample volume, 50% renewal Q9 - Inadequate sample volume, no renewal performed Q10 - Sample out of holding time Q11 - Refer to QA section of report for explanation Q12 - Supplemental information is footnoted Q13 - Test initiated with an incorrect number of test organisms Q14 - Replicate(s) not initiated Q15 - Survival counts not recorded due to poor visibility or heavy debris Q16 - Test placed on air due to DO levels dropping below 4.0 mg/L Q17 - Test initiated on air due to an anticipated drop in DO Updated 5/12/08 Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis Site: Downstream CETIS Summary Report Report Date: Link/Link Code: 20Oct-0811:30(p1of 1) 09-8060-6657/0809-S167 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: Start Date: Ending Date: Duration: Sample No: Sample Date: Receive Date: Sam pie Age: Comments: 12-7177-9760 Test Type: Reproduction-Survival (7d) 26 Sep-08 1 7: 1 5 Protocol: EPA/821/R-02-01 3 (2002) 03 Oct-08 07:35 Species: Ceriodaphnia dubia 6d 14h Source: I n-House Culture 11-7256-9626 Code: 08-0870 25 Sep-08 10:40 Material: Downstream Ambient Sample 26 Sep-08 09:55 Source: Dudek & Associates 31h(6"C) Station: Downstream Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Age: <24 Client: Dudek & Associates Project 'Shares conductivity control with Well 3, Comparison Summary Analysis No Endpoint 06-5242-3574 05-6046-9607 7d Survival Rate Reproduction Test Acceptability Analysis No Endpoint 06-5242-3574 05-6046-9607 05-6046-9607 7d Survival Rate Reproduction Reproduction NOEL LOEL 100 MOO 100 > 100 Attribute Control Resp Control Resp PMSD Test Stat 1 20.4 0.407 TOEL N/A N/A PMSD N/A 40.7% Acceptabilty Limits 0.8 - NL 15 -NL 0.13-0.47 Method Fisher Exact/Bonferroni-Holm Test Bonferroni Adj t Test Overlap Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 9 10 Mean 0.8 1 0.9 0.9 1 1 95% LCL 0.643 1 0.782 0.782 1 1 95% UCL 0.957 1 1 1 1 1 Min 0 1 0 0 1 1 Max 1 1 1 1 1 1 StdErr 0.077 0 0.0577 0.0577 0 0 Std Dev 0.422 0 0.316 0.316 0 0 CV% 52.7% 0.0% 35.1% 35.1% 0.0% 0.0% Diff% 0.0% . -25.0% -12.5% -12.5% -25.0% -25.0% Reproduction Summary Conc-% 0 0 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 9 10 Mean 3.3 20.4 24.8 20.9 23.1 16.5 95% LCL 2.38 18.5 21.1 16.8 20.5 14.8 95% UCL 4.22 22.3 28.5 25 25.8 18.2 Min 0 13 0 2 9 10 Max 6 30 34 37 37 25 StdErr 0.448 0.948 1.82 1.99 1.3 0.853 Std Dev 2.45 5.19 9.99 10.9 7.1 4.67 CV% 74.3% 25.4% 40.3% 52.2% 30.7% 28.3% Diff% 0.0% -518.0% -652.0% -533.0% -600.0% -400.0% 7d Survival Rate Detail Conc-% 0 0 12.5 25 50 100 Reproduction Conc-% 0 0 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 Rept 0 21 27 22 24 16 Rep 2 1 1 1 1 1 Rep 2 6 30 32 26 25 Rep 3 1 1 1 1 1 1 Rep 3 6 25 31 16 23 13 Rep 4 0 1 1 1 1 1 Rep 4 0 19 34 15 9 10 Rep 5 1 1 1 1 1 1 Rep 5 3 13 24 11 37 21 Rep 6 1 1 1 0 1 1 Rep 6 4 17 33 2 22 15 Rep 7 1 1 1 1 1 1 Rep 7 5 17 24 31 25 21 Reps 1 1 1 1 1 1 Rep 8 5 15 19 33 24 13 Rep 9 0 1 1 1 1 1 Rep 9 0 24 24 16. 23 13 Rep 10 1 1 0 1 1 1 Rep 10 4 23 0 37 21 18 000-089-163-3 CETIS™ v1.6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0811:30(p2of 2) 09-8060-6657/0809-S167 Cerlodaphnia 7-d Survival and Reproduction Test Analysis No: 05-6046-9607 Analyzed: 20 Oct-08 11:29 Endpoint: Reproduction Analysis: Parametric-Multiple Comparison Nautllus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Comments: "Shares conductivity control with Well 3. Data Transform Zeta Alt Hyp Monte Carlo NOEL Untransformed Bonferroni Adj t Test Control vs Conc-% Lab Control 12.5 25 50 100 C>T Not Run 100 Test Stat Critical MSD P-Value -1.23 2.32 8.3 1.0000 -0.14 2.32 8.3 1.0000 -0.738 2.32 8.52 1.0000 1.09 2.32 8.3 0.5630 ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Between 390.2009 Error 2812.289 Total 3202.49 ANOVA Assumptions Attribute Test 97.55022 4 1.53 0.2110 63.91566 44 161.4659 48 Test Stat Critical P-Value Variances Bartlett Equality of Variance 9.31 13.3 0.0537 Distribution Shapiro-Wilk Normality 0.957 0.0697 Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Mln 0 Lab Control 10 12.5 10 25 10 50 9 100 10 Graphics 10- •w fr $. 20- =•= 10- 20.4 18.4 22.4 13 24.8 21 28.6 0 20.9 16.7 25.1 2 23.1 20.4 25.8 9 16.5 14.7 18.3 10 20- 15- •n 10- 11 * • c e •^ s * o .- ,.,.. c -•- s * •10- R. jectNtli 1 -20- LOEL TOEL TU PMSD >100 N/A 1 40.7% Decision(S%) Non-Significant Effect Non-Significant Effect Non-Significant 'Effect Non-Significant Effect Dec!sion(S%) Non-Significant Effect Decision(1%) Equal Variances Normal Distribution Max StdErr Std Dev CV% Diff% 30 0:964 5.19 25.4% 0.0% 34 1.85 9.99 40.3% -21.6% 37 2.03 10.9 52.2% -2.45% 37 1.32 7.1 30.7% -13.3% 25 0.868 4.67 28.3% 19.1% ./ *s S* ' • 0 12.S 25 SO 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.S Conc-% Rankits 000-089-163-3 CETIS ""vI.Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0811:30(p1of 1) 09-8060-6657/0809-S167 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 06-5242-3574 Analyzed: 20 Oct-08 11:29 Endpoint: 7d Survival Rate Analysis: STP 2x2 Contingency Tables CETIS Version: Official Results: CETISvl.6.3 Yes Comments: 'Shares conductivity control with Well 3. Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run 100 >100 N/A N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-%Test Stat P-Value Decision(O.OS) Lab Control 12.5 25 50 100 0.5 1 Non-Significant Effect 0.5 1 Non-Significant Effect 1 1 Non-Significant Effect 1 1 Non-Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 12.5 25 50 100 Lab Control 10 9 9 9 10 0 1 1 0 0 10 10 10 9 10 Graphics j.o- 0.9: 0.8 O.J- 0.6: Q.S- 0+ 0.3-i 0.2- O.t: 0.0 25 Conc-% 50 100 000-089-163-3 CETIS™ v1.6.3revE Analyst:, CETIS Analytical Report . , -LaV3 \iS.Link/Link Code: 20 Oct-08 11:30 (pi of 2) 09-8060-6657/0809-S167 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 14-4034-6881 Analyzed: 20 Oct-08 11:30 Endpoint: Reproduction Analysis: Parametric-Two Sample CETIS Version: Official Results: ,CETISv1.6.3 Yes Comments: 'Shares conductivity control with Well 3. Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run <0 N/A 15.4% Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSO P-Value Oecision(S%) Lab Control Conductivity Control 9.42 1.73 3.15 0.0000 Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Declslon(5%) Between Error 1462.05 296.5 1462.05 16.47222 1 18 88.8 0.0000 Significant Effect Total 1758.55 1478522 19 ANOVA Assumptions Attribute Test TestStat Critical P-Value Declsion(1%) Variances Variance Ratio F 4.48 Distribution Shapiro-Wilk Normality 0.963 6.54 0.0358 Equal Variances 0.6040 Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95%LCU 95%UCL Win Max Std Err Std Dev CV%Diff% Lab Control 10 20.4 18.4 22.4 13 30 0.964 5.19 25.4% 0.0% Conductivity Co 10 3.3 2.37 4.23 0 6 0.455 2.45 74.3% 83.8% Graphics C 30- 20- 10- 10- 8- 6- 4- 2- 0 •2- -4- -6- 0cc Reject Null Conc-% •2.0 -1.5 -1.0 -0.5 0.0 O.S 1.0 Rankitj 1.S 2.0 000-089-163-3 CETIS ™v1.6.3revE Analyst. fig- Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates / Downstream Test No: 0809-S167 Cone. 6.25% Rep T T Daily Reproduction/ Survival Cone. LC Rep ~T~ 2 3 4 S 6 7 8 9 TO Analyst: Verified by: Board/ X«tM*- <fl )? P4W>| ^ tft~- •ttiSfc'::; f O OO O n ftf)c± MID •:;i;i^:;;^oo o o c aoo *•* P^ Daily R O o O O t> Q O <5 Ou^ r& eprodu ^^"rt,i~ 0^ 4 t> Ot\ £n ction/ £ f... ^ Aoo 11 S Q toif HP Survival :i:;:Si;:s '5 0 [ O Cp<r § pv*^ ?•gp o 0(1\\ *7/5 ^•) o 0 fA Total U5T~ •*D ^«^,<f\«, •J T \B% 2-T ^ V QC IV <t Total QC Cone. 50% Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 'Daily Reproduction/ Survival,,m<5 O /) Ooq X«y M iiiiplii6 o ??ooo D 0 O O ip* flHoprioop0<>«•<< IjiJH!!!!o 1 7 <*<;•?o 4t> — !H!!«!ijs) \<* \\it (410 M<S ? to— M*!i!i!«> ^Po )^>H-\5 )«•- \T~ —• m&P Q \\ 1 C) O n \o- iliiiSilHi \ '! ' — Total 4-7 3*'fl 3* 5* 'ti&L jeH,o/A QC o Rep 10 Daphnid Survival and-Keproduction Datasheet Test Species:(c. dubiayD. pulex D. magna Start Date/Time: 9/26/2008 ^(•'S End Date/Time: 10/^/2008 Cone 25% . Rep 1 2 • 3 : 4 : 5 i 6 I 7 ! 8 : 9; 10 . Daily Reproduction/ Survival , *!ii!C6f\6 00£> Ouo mmoooo 0oooo^> iilHSl!;!!o Jitt) 0o n & O & O wm <5 0 •JL O 0 ^/A 02, b (» i;iiij$!i:i!a ,- £ 4 1 to It•a.13 iliilSiiil U HJPn or>~- Ho ft7 O !!iii#!l;l| (7 O *1?fl? • 14 o l% j!!i!*!!j!! ~* Total •3-5»- 3V Ttl« VI, 5-J^ ^f•O& \io31 QC O oo ¥ Daily Reproduction/ Survival O o e? tfll Ci O '0 IM O 10 Time Fed/Test Solution Renewed (day): (0)_OlSl (11 HO5 (2)J?^2_ (3)]26O_ (411^4 6 (5)Jfe^2_ (6> /6>/ g (7M33-35 £5 Notes: Neonates for each replicate were blocked across concentrations at test initiation; d = dead; M = male; LIP = lost in progress Comments: QC Check:Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Total QC Cone. 100% Rep 1 2 3 4 5 6 7 8 9 TO Daily Reproduction/ Survival $*; o Pdf O&o £> V•\%o !i!i|*j!!!lV& G O O J!> O ROr> ;i;i;^Hi; QOo O r\ O D O O b ;&&*! S^a. S Oo Hz 1 0 \prnto $32ei g aO 0 D i;iii*i;i;i I1-^ oi D 1 OZic>l_ 0*t !i!i!5li!i!ii O ;o tf M (i. £3<i\\ T_ 1 j!ii!*!i!!i Total Aid ^_S\3 Jy ?^JS '&A H'^\3 l^ QC il Water Quality Measurements Client: Sample ID: Test No: Dudek & Associates Test Species: C. dubia Downstream Start Date/Time: 9/26/2008 \ "~| 0809-S167 End Date/Time: 10J2J2008 Animal Source/Date Received: Animal Age at Initiation: ' Sample Log-in Numbers: A: B: Comments: QC Check: C: Analysts: Initial: Final: Sample Used (A. B, C): Mfl 7$r MO w ££.ss Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego. CA 92121. Site: Upstream CETIS Summary Report Report Date: Link/Link Code: 29Oct-0816:36(p1of 1) 05-9691-1761/0809-S166 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 00-5754-2123 Test Type: Reproduction-Survival (7d) Start Date: 26 Sep-08 17:00 Protocol: EPA/821/R-02-013(2002) Ending Date: 03 Oct-08 08:20 Species: Ceriodaphnia dubia Duration: 6d 15h Source: In-House Culture Sample No: 05-7422-3554 Code: 08-0871 Sample Date: 25 Sep-08 11:00 Material: POTW Effluent Receive Date: 26 Sep-08 09:55 Source: Dudek & Associates Sample Age: 30h{6*C) Station: Upstream Nautilus Environmental (CA) Analyst: Diluent: Not Applicable Brine: Not Applicable Age: <24 Client: Dudek & Associates Project: Comments: *Shares Conductivity Control with Well 3. Comparison Summary Analysis No Endpoint 15-2514-9502 7d Survival Rate 16-2790-6797 Reproduction Test Acceptability Analysis No Endpoint 15-2514-9502 7d Survival Rate 16-2790-6797 Reproduction 16-2790-6797 Reproduction 7d Survival Rate Summary Conc-% Control Type Count 0 Conductivity C 10 0 Lab Control 10 12.5 10 25 10 50 10 100 10 Reproduction Summary Conc-% Control Type Count 0 Conductivity C 10 0 Lab Control 10 12.5 10 25 10 50 10 100 10 7d Survival Rate Detail Conc-% Control Type Rep 1 0 Conductivity Co 1 0 Lab Control 1 12.5 0 25 1 50 1 100 1 Reproduction Detail Conc-% Control Type Rep 1 0 Conductivity Co 0 0 Lab Control 15 12,5 0 25 22 50 23 100 17 NOEL 100 > 50 Attribute Control Resp Control Resp PMSD Mean 95% LCL 0.8 0.643 1 1 0.8 0.643 1 1 0.9 0.782 0.9 0.782 Mean 95% LCL 3.3 2.38 20 17.4 18.7 14.3 24 22.3 21.6 19.2 11.9 9.46 Rep 2 Rep 3 1 1 1 1 1 1 1 1 1 1 1 1 Rep 2 Rep 3 6 6 27 32 22 21 32 24 30 26 3 17 LOEL 100 100 Test Stat 1 20 0.377 95% UCL 0.957 1 0.957 1 1 1 95% UCL 4.22 22.6 23.1 25.7 24 14.3 Rep 4 0 1 0 1 1 1 Rep 4 0 27 0 24 28 16 TOEL N/A 70.7 PMSD N/A 37.7% Acceptabilty Limits 0.8 - NL 15 -NL 0.13-0.47 Min 0 1 0 1 0 0 Min 0 10 0 17 8 0 Rep 5 1 1 1 1 0 1 Rep 5 3 14 23 22 8 18 Max 1 1 1 1 1 1 Max 6 32 35 32 30 18 Rap 6 1 1 1 1 1 1 Rep 6 4 10 19 19 17 18 Method Fisher Exact/Bonferroni-Holm Test Dunneffs Multiple Comparison Test Overlap Yes Yes Yes StdErr 0.077 0 0.077 0 0.0577 0.0577 Std Err 0.448 1.26 2.14 0.807 1.16 1.19 Rep 7 1 1 1 1 1 1 Rep 7 5 20 23 26 25 12 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Std Dev CV% Diff% 0.422 52.7% 0.0% 0 0.0% -25.0% 0.422 52.7% 0.0% 0 0.0% -25.0% 0.316 35.1% -12.5% 0.316 35.1% -12.5% Std Dev CV% Diff% 2.45 74.3% 0.0% 6.9 34.5% -506.0% 11.7 62.8% -467.0% 4.42 18.4% -627.0% 6.38 29.5% -555.0% 6.52 54.8% -261.0% Rep 8 Rep 9 Rep 10 1 0 1 1 1 1 1 1 1 1 11 1 1 1 0 1 1 Rep 8 Rep 9 Rep 10 504 20 20 15 32 35 12 29 25 17 21 20 18 0 10 8 000-089-163-3 .CETIS™ v1.6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code: 29Oct-08 16:36 (p 1 of 1) 05-9691 • 1761 /0809-S166 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 15-2514-9502 Analyzed: 20 Oct-08 12:11 Endpoint: 7d Survival Rate Analysis: STP 2x2 Contingency Tables CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run 100 >100 N/A N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-%TestStat P-Value Decision(O.OS) Lab Control 12.5 25 50 100 0.237 0.947 1 1 0.5 1 0.5 1 Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Data Summary Conc-% Control Type No-Res p Resp Total 0 12.5 25 50 100 Lab Control 10 8 10 9 9 0 2 0 1 1 10 10 10 10 10 7d Survival Rate Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep S Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 12.5 25 50 100 Lab Control 1 0 1 0 0 Graphics 1.0- 0.9- 0.8- 0.7- 0.6- 0.5- 0> 0.3- 0.2- 0.1- 0.0 12.5 25 Conc-% 000-089-163-3 CETIS™ v1.6.3revE Analyst CETIS Analytical Report Report Date: Link/Link Coda: 29 Oct-08 16:36 (p 1 of 1) 05-9691-1761/0809-S166 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 16-2790-6797 Endpoint: Reproduction Analyzed: 29 Oct-08 16:35 Analysis: Parametric-Control vs Treatments Data Transform Zeta Alt Hyp Untransformed C > T Dunnetfs Multiple Comparison Test Control vs Conc-% TestStat Lab Control 12.5 0.383 25 -1.18 50 -0.471 100* 2.39 ANOVA Table Source Sum Squares Mean Square Between 829.72 207.43 Error 2593.4 57.63111 Total 3423.12 265.0611 ANOVA Assumptions Attribute Test Variances BartJett Equality of Variance Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count Mean 0 Lab Control 10 20 12.5 10 18.7 25 10 24 50 10 21.6 100 10 11.9 Reproduction Detail Conc-% Control Type Repl Rep 2 0 Lab Control 32 27 12.5 35 32 25 32 29 50 30 28 100 18 18 Graphics w- • • , \ • ; § 30- i j i j [--•--. 2 *•- r+- j-»v- j t^ I j : j . - - j - 10- • ; [ 0 12.5 25 50 Conc-% Monte Carlo Not Run Critical 2.22 2.22 2.22 2.22 DF 4 45 49 Test Stat 9.07 0.973 95% LCL 17.4 14.2 22.3 19.2 9.42 Rep 3 27 23 26 26 17 i - Rc^wTMJH '• : 100 MSD 7.55 7.55 7.55 7.55 FStat 3.6 Critical 13.3 95% UCL 22.6 23.2 25.7 24 14.4 Rep 4 20 23 25 25 17 NOEL 50 P-Value 0.6540 0.9860 0.9180 0.0349 P-Value 0.0125 P-Value 0.0594 0.3080 Min 10 0 17 8 0 Rep 5 20 22 24 23 16 20- 15- 1 10- 11 , SJ . -s- -10- -15- tn- CETIS Version: Official Results: LOEL TOEL 100 70.7 Decisfon(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision(5%) Significant Effect Decfsion<1%) Equal Variances Normal Distribution Max Std Err 32 1.28 35 2.18 32 0.821 30 1.18 18 1.21 Rep 6 Rep 7 20 15 21 19 24 22 21 20 12 10 ^^ : Nautilus Environmental (CA) CETISv1.6.3 Yes TU PMSD 2 37.7% Std Dev CV% Diff% 6.9 34.5% 0.0% 11.7 62.8% 6.5% 4.42 18.4% -20.0% 6.38 29.5% -8.0% 6.52 54.8% 40.5% Rep 8 Rep 9 Rep 10 15 14 10 12 0 0 22 19 17 18 17 8 8 30 ^ -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report . -\j£ » - , - Lab Ay<3%~ CoOckACTTsA" Cerlodaphnia 7-d Survival and Reproduction Test £G> «^ vT D Analysis No: 10-7215-8558 Endpoint: Reproduction Analyzed: 20 Oct-08 12:13 Analysis: Parametric-Two Sample Report Date: 20 Oct-08 1 2: 1 3 {p 1 of 2) TV! Link/Link Code: 05-9691-1761/0809-S166 ' Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes Comments: 'Shares Conductivity Control with Well 3. Data Transform Zeta Alt Untransformed C > Hyp Monte Carlo T Not Run Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD Lab Control Conductivity Control 7.22 1.73 4.01 ANOVA Table Source Sum Squares Mean Square DF F Stat Between 1394.45 1394.45 1 52.1 Error 482.1 26.78333 18 NOEL LOEL TOEL TU PMSD <0 0 N/A 20.1% P-Value Decision<5%) 0.0000 Significant Effect P-Value Decision(5%) 0.0000 Significant Effect Total 1876.55 1421.233 19 ANOVA Assumptions Attribute Test Variances Variance Ratio F Distribution Shapiro-Wilk Normality TestStat Critical 7.91 6.54 0.964 Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL 0 Lab Control 10 20 0 Conductivity Co 10 3.3 Graphics 40- | 30" f1 20" 10- 0 GC- Conc-% 17.4 22.6 2.37 4.23 • 0 LC P-Value Decision(1%) 0.0050 Unequal Variances 0.6240 Normal Distribution Min Max Std Err Std Dev CV% Diff% 10 32 1.28 6.9 34.5% 0.0% 0 6 0.455 2.45 74.3% 83.5% • 10- s O a -/• • 5 ^* «^» -5- *^* -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Ranklts 000-089-163-3 CETIS™ v1.6.3revE Analyst:. Ciuonic Btudssay Client/Sample ID: Dudek & Associates / Upstream Test No: 0809-S166 Cone. LC Rep •T 2 3 4 5 6 7 8 9 10 Analyst: Verified by: Board/ Cup GJ ^— •£•<-•*te* Daily Reproduction/ Survival ;#!&#; 0o t)0 0o(\0 0o MO ;;;ii;2hi:itf>booo ooo oo rP" i:^3*!; O ?' oot?o 9 ISw 2. 6 e> H0 £?Hf;t, o €5 !;fij$j!jij 2. ^jh?n Ar O *7 4*£• \^4r NiliWIili tl & c, f) rt (t H tlrf !!:;;!^h!:; o op ra 4 CDc; Ofj c< ;;;:!8;;i;l i Total \5 21 2." ^U\P 5?<? JO 3>O VS QC ~> & *c Cone. 6.25% Rep tA- Hit!! t 0 O Daily Reproduction/ Survival Cone. 12.5% Rep 1 2 3 4 5 6 7 [ 8 9 10 Daily Reproduction/ Survival. Mm 08bo$^o A £) O l|:j|i^;;:|; 0/&rD{? o D O O& 0 D :|;i|3|jii| -- O O O ^O O !l|i|j|!j!j! , g 5 O/ck ^• -5 2. f> ,-, £> j|iii!$!iii! —^C"4j. 69 /?aiif?<? lililSliji!^ i>- A —0H-1 ^''» *•*) i|!li!jj:j!;lj ^ dD i-2. — iulii7_£>o -7 liiilg;;;;: — -~ Total O/A. 3J. 94 CTO _9-S l^ JL^32-a,i~ \'i QC O Daphnid Survival and Reproduction Datasheet Test Species :^C. dubia^D. pulex D. magna Start Date/Time: 9/26/2008 )1 OO End Date/Time: 10J2/2008 Con 25° c. Rep 1 4 2 : 3 j 4 i 5 ; 6 I 7 i 8 ; 9 •j TO Daily Reproduction/ Survival im #CJ O 0 ^C? #50 O Hi!*!*o O Ooooo o<?o !i!i!9!!£o ^0 0 0e>o f?H-p llliiHUIilJ_s _,p u c 5 I 4 5o b ijljiS!!!!! (pY4a4- fy Qf JLy •i- i!i;!#!i!!Uniif0C-* \fl <4-3— nm&o r> oO .1. \ tbn0o !!!!!*!!$ M Total 7-T 3»5- 243^7t ?•>•vfifey\ i"i QC ^2_ Daily Reproduction/ Survival Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival im6or>o 0 06 0/) P mm0 o fto 0 0 f*o o o !liii3!i!i;O Oo 0 0 3^) O0o :!=i;=4ji:!i: 3 0 3 M i-1 D 2-, 0/4 0u $!#!$t? r2 ^^> 7 <J12> —or^> !!!=i*!lii2- V ^o !-» dp« h;i;i7ii;i!i ?'^ O1 •70 ^- •<?0 i^i*^i >«x Total rj??"7 <<s^\« »oi. fit/**P? QC ^ Time Fed/Test Solution Renewed (day): (OjJ^^O. M> IM&U60 (4i \M\(51 <6) (j)^ (7)_ Notes: Comments: QC Check: Neonates for each replicate were blocked across concentrations at test Initiation; d = dead; M = male; LIP = lost in progress Final Review: Nautilus Environmental 5550 More/louse Drive, So/re 150. San D/ego, CA 92121. Water Quality Measurements Client: Sample ID: Test No: Dudek & Associates Upstream 0809-S166 Concentration Day pH DO (mg/U) Cond. (pmhos/cm) Temp (°C) T''""/. .' f... V.>>' N^'/ •- • pH 00 (mg/L) Temp (*C) Lab Control 517- 24.0 <?• :.o 13514--2. ..« 1 7.. ' ^'FfaBl'" \ \j Is . 8.01 -3 STas7 8.1 Concentration Day PH DO (mg/L) Cond. (pmhos/cm) Temp(°C) .»» ' '.H '•;"' PH DO (mg/L) Temp('C) 6.25% <3\?_tJ-f 0 ijJsUL' ».^. X^l• »* -2Q.O4 ' ;5 ^ .. s ; "i-;"^ 1 \. .* ' ZH.(# 2 ----. 3 ... .;....» "^-^, ... J=S 4 «*.': --^ ^-v. nat 5 I 6 •^^ """^^ 7 \ , ^ -.% x n ^>^^ Concentration Day DO (mg/L) Cond. (nmhos/cm) Tamp ("CJ PH DO (mg/L) Temp (°C) 12.5% 8-Zo flA E •7$ 1 .•-I/---*™* • .] LIOZ. Animal Source/Date Received: Animal Age at Initiation: Sample Log-In Numbers: Test Species: C. dubia Start Date/Time: 9/26/2008 |~7O° End Date/Time: 10/272008 .0 Concentration Da pH DO (mg/L) Cond. ((Jmhos/cm) Temp('C) PH DO (mg/L) Temp CC) 100% J—*. a?. ^8 ? /fflftal 4? * I *,,,!.., '...j, 7, p»^v": 8-1 . S- Analysts: Initial: Final: Sample Used (A, B, C): MD HP J3b IAD A B: Comments: QC Check: G47-. - Final Review:/o/g.y/o»> Nautilus Environmental 5550 Warehouse Drive, Suite ISO. San Diego. C4 92Y24. f f • 1 Site: Well #3 CET1S Summary Report Report Date: Link/Link Code: 20 Oct-08 10:55 (p 1 of 2) 16-0435-7598/0809-S169 Ceriodaphnla 7-d Survival and Reproduction Test Test Run No: 09-7174-3259 Test Type: Reproduction-Survival (7d) Start Date: 26 Sep-08 16:30 Protocol: EPA/821 /R-02-01 3 (2002) Ending Date: 03 Oct-08 09:20 Species: Ceriodaphnia dubia Duration: Gd 17h Source: In-House Culture Sample No: 07-8026-2283 Code: 08-0872 Sample Date: 25 Sep-08 12:00 Material: Groundwater Receive Date: 26 Sep-08 09:55 Source: Dudek & Associates Sample Age: 28h (6 °C) Station: Well 3 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: ^LU^<50f Client: Dudek & Associates Project: Comments: "Neonates were collected from 1mae» culture known to be 24 hours or younger. Comparison Summary Analysis No Endpoint 02-2 1 29-1 246 7d Survival Rate 17-7538-3137 Reproduction Point Estimate Summary Analysis No Endpoint 03-6843-3872 7d Survival Rate 19-0882-8068 Reproduction Test Acceptability Analysis No Endpoint NOEL 50 <12.5 Effect-% 50 50 Attribute 02-2 1 29-1 246 7d Survival Rate 03-6843-3872 7d Survival Rate 17-7538-3137 Reproduction 1 9-0882-8068 Reproduction 1 7-7538-3 1 37 Reproduction 7d Survival Rate Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 12.5 25 50 100 Reproduction Summary Conc-% Control Type 0 Conductivity C 0 Lab Control 12.5 25 50 100 Count 10 9 9 10 JO 10 Count 10 9 9 10 10 10 Control Resp Control Resp Control Resp Control Resp PMSD Mean 0.8 1 1 1 0.8 0.4 Mean 3.3 21.8 14.6 13.2 7 0.4 95% LCL 0.643 1 1 1 0.643 0.207 95% LCL 2.38 19.3 13.5 12.5 5.43 0.0393 LOEL 100 12.5 Conc-% 84.1 34.3 Test Stat 1 1 21.8 21.8 0.184 95% UCL 0.957 1 1 1 0.957 0.593 95% UCL 4.22 24.2 15.6 13.9 8.57 0.761 TOEL 70.7 N/A PMSD N/A 18.4% Method Fisher Exact/Bonferroni-Holm Test Wilcoxon/Bonferroni Adj Test 95% LCL 95% UCL Method 55.4 24.8 128 45.8 Trimmed Spearman-Karber Linear Interpolation (ICPIN) Acceptabilty Limits Overlap 0.8 - NL 0.8 - NL 15 -NL 15-NL 0.13-0.47 Min 0 1 1 1 0 0 Min 0 10 11 11 0 0 Max 1 1 1 1 1 1 Max 6 31 19 17 13 3 Yes Yes Yes Yes Yes StdErr 0.077 0 0 0 0.077 0.0943 StdErr 0.448 1.21 0.501 b.331 0.77 0.176 Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria StdDev 0.422 0 0 0 0.422 0.516 Std Dev 2.45 6.61 2.74 1.81 4.22 0.966 CV% 52.7% 0.0% 0.0% 0.0% 52.7% 129.0% cv% 74.3% 30.4% 18.8% 13.7% 60.2% 242.0% Diff% 0.0% -25.0% -25.0% -25.0% 0.0% 50.0% Dirf% 0.0% -560.0% -341.0% -300.0% -112.0% 87.9% 000-089-163-3 CETIS™v1.6.3revE Analyst: CETIS Summary Report Report Date: Link/Link Code: 20Oct-Q810:55(p2of 2) 16-0435-7598/0809-S169 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 0 Rep 2 1 1 1 1 0 Rep 3 1 1 1 1 1 0 Rep 4 0 1 1 1 0 0 Rep 5 1 1 1 1 1 1 Rep 6 1 1 1 1 1 1 Rep 7 1 1 1 1 1 1 Rep 8 1 1 1 1 1 0 Rep 9 0 1 1 1 1 0 Rep 10 1 1 1 0 1 Reproduction Detail Conc-% 0 0 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 0 23 11 13 8 0 Rep 2 6 31 13 5 0 Rep 3 6 10 15 13 7 0 Rep 4 0 25 19 11 2 0 Rep 5 3 23 12 11 13 3 Rep 6 4 15 17 15 11 0 Rep 7 5 28 13 17 5 0 Rep 8 5 24 17 14 .12 1 Rep 9 0 17 12 13 7 0 Rep 10 4 15 12 0 0 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0810:54(p2of 2) 16-0435-7598/0809-S169 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 02-2129-1246 Analyzed: 20 Oct-08 10:51 Endpoint: 7d Survival Rate Analysis: STP 2x2 Contingency Tables CETIS Version: CETISv1.6.3 Official Results: Yes Comments: *Neonates were collected from efrmeaa culture known to be 24 hours or younger. Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run 50 100 70.7 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-%TestStat P-Value Decision(O.OS) Lab Control 12.5 25 50 100 1 1 Non-Significant Effect 1 1 Non-Significant Effect 0.263 0.789 Non-Significant Effect 0.00774 0.031 Significant Effect Data Summary Conc-% Control Type No-Resp Resp Total 0 12.5 25 50 100 Lab Control 10 8 4 0 0 0 2 6 9 10 10 10 Graphics 1.0: 0.9- u O.fl- a »> •| 0,6- W 0.5-s 0.3r 0.2- 0.1- 0.0 12.5 25 Conc-% 50 100 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 20 Oct-08 10:54(p 1 of 1) 1 6-0435-7598/0809-S 1 69 Ceriodaphnia 7-d Survival and Analysis No: Analyzed: Comments: 03-6843-3872 20 Oct-08 10:53 Reproduction Test Endpoint: 7d Survival Rate Analysis: Trimmed Spearman-Karber CETIS Version: Official Results: Nautilus Environmental (CA) CET1SV1.6.3 Yes 'Neonates were collected from orrTagj culture known to be 24 hours or younger. Spearman-Karber Estimates Threshold Option Threshold Control Threshold 0 7d Survival Rate Summary Conc-% Control Type 0 Lab Control 12.5 25 50 100 Trim 40.00% Mu 1.92 Sigma 0.0906 EC/LC50 84.1 95% LCL 55.4 95% UCL 128 Calculated Variate(A/8) Count 9 9 10 10 10 Mean 1 1 1 0.8 0.4 Win 1 1 1 0 0 Max Std Err 1 0 1 0 1 0 1 0.077 1 0.0943 Std Dev 0 0 0 0.422 0.516 CV% 0.0% 0.0% 0.0% 52.7% 129.0% Diff% A 8 0.0% 9 9 0.0% 9 9 0.0% 10 10 20.0% 8 10 60.0% 4 10 7d Survival Rate Detail Conc-% Control Type 0 Lab Control 12.5 25 50 100 Graphics l.CH 0.8- $ 0.7- _ 0.5-5 'j> 0.5: " 0.4-is 0.3- 0.2- 0.1- 0.0 ) 20 40 Rep1 1 1 1 1 0 X \ 60 Conc-% Rep 2 1 1 1 1 0 \ 80 Rep 3 1 1 1 1 0 •* tOO 120 Rep 4 Rep 5 1 1 1 1 1 1 0 1 0 1 ' Rep 6 1 1 1 1 1 Rep 7 1 1 1 1 1 Rep 8 Rep 9 Rep 10 1 1 1 1 1 1 1 1 1 0 0 0 1 000-089-163-3 CETIS™ v1.6.3revE Analyst:, CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0810:54(p2of 3) 16-0435-7598/0809-S169 Cerlodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-7538-3137 Endpoint: Reproduction Analyzed: 20 Oct-08 10:52 Analysis: Nonparametric-Multiple Comparison CETIS Version: CETISvl Official Results: Yes .6.3 Comments: 'Neonates were collected from a mass culture known to be 24 hours or. younger. Data Transform Zeta Alt Hyp Rank C > T Wllcoxon/Bonferroni Adj Test Control vs Conc-% TestStat Lab Control 12.5' 60 25* 67 50* 58 ; 100* 55 ANOVA Table Source Sum Squares Mean Square Between 2490.701 622.6754 Error 607.7778 14.13437 Total 3098.479 636.8097 ANOVA Assumptions Attribute Test Variances Bartlett Equality of Variance Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count Mean 0 Lab Control 9 21.8 12.5 9 14.6 25 10 13.2 50 10 7 100 10 0.4 Rank Transformed Summary Conc-% Control Type Count Mean 0 Lab Control 9 40.7 12.5 9 32.3 25 10 29.2 50 10 16.6 100 10 6.2 Monte Carlo Not Run Critical DF 4 43 47 TestStat 30.1 0.952 95% LCL 19.3 13.5 12.5 5.4 0.0325 95% LCL 37.1 29.4 26.8 13.9 5.22 Ties 2 2 0 0 FStat 44.1 Critical 13.3 95% UCL 24.3 15.6 13.9 8.6 0.767 95% UCL 44.2 35.3 31.5 19.2 7.18 NOEL <12.5 P-Value 0.0367 0.0085 0.0002 0.0000 P-Value 0.0000 P-Value 0.0000 0.0486 Win 10 11 11 0 0 Min . 18 20.5 20.5 5 5 LOEL TOEL TU 12.5 N/A 78.0 Decision(5%) Significant Effect Significant Effect Significant Effect Significant Effect Decision(5%) Significant Effect Decision(1%) Unequal Variances Normal Distribution Max Std Err Std Dev 31 1.23 6.61 19 0.509 2.74 17 0.337 1.81 13 0.783 4.22 3 0.179 0.966 Max Std Err Std Dev 48 1.74 9.35 42 1.45 7.8 39.5 1.13 6.09 29.5 1.29 6,93 12 0.478 2.57 PMSD 18.4% CV% Diff% 30.4% 0.0% 18.8% 33.2% 13.7% 39.4% 60.2% 67.9% 242.0% 98.2% •CV% Diff% 23.0% 0.0% 24.1% 20.5% 20.9% 28.3% 41.8% 59.3% 41.5% 84.8% 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0810:54(p3of 3) 16-0435-7598/0809-S169 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-7538-3137 Endpoint: Reproduction Analyzed: 20 Oct-08 10:52 Analysis: Nonparametric-Multiple Comparison CETIS Version: CETISvl .6.3 Official Results: Yes Graphics 20- 10- JO.[ 1 I—• '—I—' •»• •—I 0 12.5 25 50 100 Conc-% 15- 10- 5- 0 -5- -10- -15- -20 -25 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Rankits 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 20Oct-0810:54(p1of 1) 16-0435-7598/0809-S169 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 19-0882-8068 Endpoint: Reproduction Analyzed: 20 Oct-08 10:53 Analysis: Linear Interpolation (ICPIN) Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Comments: 'Neonates were collected from a mass culture known to be 24 hours or younger. Linear Interpolation Options X Transform Y Transform Seed Resamples Linear Linear 7055475 280 Exp 96% CL Method Yes . Two-Point Interpolation Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 34.3 24.8 45.8 Reproduction Summary Conc-% Control Type Count 0 Lab Control 9 12.5 9 25 10 50 10 100 10 Reproduction Detail Conc-% Control Type Rep 1 0 Lab Control 23 12.5 . 11 25 13 50 8 100 0 Graphics 25- 1 20- 1 " t 10- s- 0 ( V 20 40 60 Conc-% Calculated Variate Mean Min 21.8 10 14.6 11 13.2 11 7 0 0.4 0 Rep 2 Rep 3 31 10 15 19 13 13 5 7 0 0 >N... ,80 100 120 Max 31 19 17 13 3 Rep 4 25 12 11 2 0 StdErr 1.21 0.501 0.331 0.77 0.176 Rep 5 23 17 11 13 3 Std Dev CV% Diff% 6.61 30.4% 0.0% 2.74 18.8% 33.2% 1.81 13.7% 39.4% 4.22 60.2% 67.9% 0.966 242.0% 98.2% Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 15 28 24 17 13 17 12 15 15 17 14 13 12 11 5 12 7 0 00100 000-089-163-3 CETIS™ vl .6.3revE Analyst;:££. CETIS Analytical Report Report Date: Link/Link Code: 200ct-0810:54(p1 of 3) 16-0435-7598/0809-S169 Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 19-4657-2987 • Endpoint: Reproduction Analyzed: 20 Oct-08 10:53 Analysis: Parametric-Two Sample CETIS Version: CETISvl .6.3 Official Results: Yes Comments: 'Neonates were collected from aPrnao* culture known to be 24 hours or younger. Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run <0 N/A 19.7% Unequal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Decislort(5%) Lab Control Conductivity Control 7.91 1.83 4.28 0.0000 Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(5%) Between Error 1617.292 403.6555 1617.292 23.74444 1 17 68.1 0.0000 Significant Effect Total 2020.947 1641.036 18 ANOVA Assumptions Attribute Test TestStat Critical P-Value Decision(1%) Variances Variance Ratio F 7.27 Distribution Shapiro-Wilk Normality 0.945 6.69 0.0075 Unequal Variances 0.3270 Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Win Max Std Err Std Dev CV%Diff% Lab Control 9 21.8 19.3 24.3 10 31 1.23 6.61 30.4% 0.0% Conductivity Co 10 3.3 2.37 4.23 0 6 0.455 2.45 74.3% 84.8% Graphics •tO-i 30- 20- 10- lO-i 5- -5- -10- •15- Conc-% •2.0 -1.S -1.0 -0.5 0.0 0.5 1.0 l.S 2.0 Rankits 000-089-163-3 CETIS™ v1.6.3revE Analyst:. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates / Well 3 Test No: Cone. LC Rep ^2 3 4 5 6 7 8 9 10 Analyst: Verified by: Board/ Cup A\s»- xif 0809-S169 Daily Reproduction/ Survival .:'::i:ft:::::i) ®oQ • 000 A Mn OoO O O0oo 0o £4» o 0 0o D Do *L» ^'O fcjp I:|:!i4i;!:!ii^ oI T- 0 5 0o Eft ^;.:!:$:;:-::e>TJ '••• l^o O H'6 \03o Mft S:;;: 4 « •L LO C( \\ <k \"t-\\ N\•^c> ;;;;:i?!;i;:ji_( $ ^is \7- 0 iH0 o Cr\ &> :;:;;i8:i:i:Total •-'•33 1jA 4^1 JS"^*tn QC ILV*- Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 : Daily Reproduction/ Survival 1#i! rt -A0o7o <3 Ooooo il:|ii*i!ii! oe> F>— Oooooo W®"o oo - oono 0o mmo«>o ^_ d 6 6ft t50 !!J!iSi!!!ia yLo^at 1iotoo jiSSiiijiooo_ <*> •>> H 3,«• O iiilliriiii! f> ^ ^^_ oo n O— »-\ iiiiiKiiii! -^ — Total O If b 0/P3 H5- S" O/P4 QC UV.X- Cone. 6.25% Reft 1 2 3 4 5 6 7 8 9 1O -»*_ -"^ Daily Reproduction/ Survival -iliiii -~--^ iiiiiiZiiil. ~^«^_ iiliiS,!;!!; ^~««^_ :ji!!i4:^i *^^ j!;!$i* ^ — ^ i!i|i»!Hi! "^^-^.^ =!!!!!«!!!!! "" — »^_ !ii!iS;!i!i " ~^_ Total ~~\^ QC ^-— ^. Daphnid Survival andRggroduction Datasheet Test Species: <£dubia^>D. pulex D. magna Start Date/Time: 9/26/2008 End Date/Time: 10f2f/20Q8 Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival f O Q 0 rVO 0 A V ~0"~ ~ z ooo<?oooo'&(J 3 <2 rt O D 0Uu"i-oo 4. 5» Ht i1. S&92 5/£0•i. 5 S t) sj o •?• 0 O4 T V 6•toUf•R lo. uu pi4T 7 : i? rt pt; q, •7 (? P0 a -N Total L I 1 W' A?Iti1_nphj^-tsr QC ' °VU, M <2)(4>/32-6>Time Fed/Test Solution Renewed (day): i Notes: Neonates for each replicate were blocked across concentrations at test initiation; d = dead; Ml = male; LIP = lost in progress Comments: QC Check: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Cone. 25% ; Rep i 2 3 4 S 6 7 8 9 10 Daily Reproduction/ Survival Hiil O A6 Vono Q Oo i!!i;!«i!i|! 005or\aoo G C/ itf*d 0 <5 <7O niff $£®o u piiiijiiii! •i0 e 0 5 "i 2>H H :lli!;Sl!ili Ot ^ i- O b o ^5 2. ISiiH S .5 ^ c^ 0-1. ^o "7 "O i|;|=|^i:i| £ n i/&- "3L ^ ^O 6 |J|^P|i! Total IH I-J J^ftn L^ni413 11- QC MV^ Cone. 50% Rep ^2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival <!|!iia<j Oo f\uoft iffOb mmooc» o Qd S6u6 iiHi&lili£>o O o 0 Uo sl-l> Q,0 !i!ii*iH! « 0 0 00 0 0 0 S 'a mm\<3o <3 £M6 V?I .£fi(/ #$& oo•A f~ i n vV o> Ci c.)«\ |!!;|*iii;i<So _ o Q.p fl2 — iiiillii!!! . — . — Total "7 i-/P V?M S'»«-TolU QC ^\pw- Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates/Wells Test Number: 0809-S169 Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 9/26/2008 i End Date/Time: "I0f2fa008 (WO Cone. 100 Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup <3 v)' \$~ Daily Reproduction/ Survival !ji!H3£ii;j <L> €> OO/D <2 Q O Q 0 (' H) :i£l:Z£h! O O 0 — O O 0 O O O£p :!!&£!!£O O 0— O 0aooo VSF- m$m 0 0 a _ 6 ,f ,-\ I 0 0& ii!iii3?i!i;lli rsr o/D O . — v3& eD O o/O O I-*D !!i!ii5!l!i!l;o/of — — •d —f\ 0 O-o^— <9 SD j:;i^;:!;^i:;:;:®;;i^ ~ ****0/& — -1 "rt Oo-~ —o e-s ~— — • Total 0/P 0/17 £V(7 O/O J3Oe> l/C>o/uo QC £> tx- Cone. iiiiii!;;! ; iii : '::i-:!'i : '•: -.:-:. Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival fa%!:j:!:i?j:!:|:::!:!:3:i:(:i •;;:;:;Si:i:;:!:|:!:S||:ii!-:j:!:|fl[:|:i:!:ll:|:=J::iii:ii :!:|:i®i!:|:! Total Cone.Rep 1 2 3 4 5 6 7 8 9 10 ,_ Daily Reproduction/ Survival 1:::j|jj :£j;:Z:*::::i:=|i3::::;::::::;14=:=i:::ii!l|5!:|i|i !jj::|iS:|:!iji ;:;j;::7i;;-;:;ji!!|:j8:;:j;i:Total Cone.Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1;|i:::j ijijIijZ;;;:;!!i:i|:i>5|:|:|:!:j:!:;4i:i:;:iSilrjiSj:;:!:mm*r|:!:;?l;!;!:!:!:|;®!:;i!i Total QC QC QC Time Fed/Test Solution Renewed (day): (5)(7) Notes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) Comments: Q\ 3--C.G C QC Check:Final Review: fit/ Nautilus Environmental 5550 Morehouse Drive, Suits 150. San Diego, CA 92121. Freshwater Chronic Bioassay Dudek & AssociatesClient: Sam pie ID: Test No: Well 3 0809-S169 Concentration Day J*LD0(mgfl.) Cond. (pmhos/cm) ^Temp('C) -I". DO(mg/L) Temp CC) 6.25% *«==. t*. s n r fina* ' Animal Source/Date Received: Animal Age at Initiation: A/A Water Quality Measurements Test Species: C. dubia Start Date/Time: 9/26/2008 End Date/Time: 1 C: Analysts: Initial: Final: Sample Used (A, B, C): MO 11^) Sample Log-in Numbers: A: Oft - iL4 QJt. " VXOV €.«\QJug^yN cg^^^^flAa; -^"g t\\JLVraL^<- fc*^ C(T.v^ VvgJv-A-^"^^ DF Comments: QC Check: Nautilus environmental 5550 Monhouse Drive, Suite ISO. San Diego, CA 92121. Final Review: Freshwater Chronic Bioassay Client: Dudek & Associates Water Quality Measurements Test Species: C. dubla Sample ID: Well 3 Start Date/Time: 9/26/2008 Test No: 0809-S169 End Date/Time: 1 0 J?2008 D °[ 3- b PH DO (mq/L) Cond. (pmhos/cm) Temp *C)3& pH Temp (*C) Concentration Da 8.5-.^ TT-TT 2 r » i 4 PH DO (man.) Cond. (^mhos/cm) Tem -C) PH DO (mg/t) Tem (°C) Concentration Day i ,1. i i i ,», .1 pH DO (mq/L) Cond. (jjmhos/cm) Temp (°C) PH DO (mp/L) Temp ° Analysts: Initial: Final: Sample Used (A, B, C}: Comments: MP ^MO A *PT^-P^ Animal Source/Date Received: V\ £>«=» Sample Log-In Numbers: A:08 ~ QC Check: B: Animal Age at Initiation:. C: . — Final Review: Nautilus Environments/ SfSO Morehouse Drive, Suite f50. San 0/ego, CA 9?f ZT. Ur-S Site: Well#1 CETIS Summary Report Report Data: Link/Link Code: 290ct-0817:17(p1of 2) 08-7802-0912/0809-S168 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: Start Date: Ending Date: Duration: Sample No: Sample Date: Receive Date: Sample Age: Comments: 00-2477-2611 Test Type: Reproduction-Survival (7d) 26 Sep-08 16:30 Protocol: EPA/821/R-02-013 (2002) 03 Oct-08 13:25 Species: Ceriodaphnia dubia 6d 21 h Source: In-House Culture 14-3185-0288 Code: 08-0873 25 Sep-08 15:30 Material: Groundwater 26 Sep-08 09:55 Source: Dudek & Associates 25h (6 *C) Station: Well 1 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24 Client: Dudek & Associates Project: •Shares Conductivity Control with Well 3. Comparison Summary Analysis No Endpoint 15-3424-7594 10-8290-1386 7d Survival Rate Reproduction Point Estimate Summary Analysis No Endpoint 17-4057-6126 Reproduction Test Acceptability Analysis No Endpoint 15-3424-7594 10-8290-1386 17-4057-6126 10-8290-1386 7d Survival Rate Reproduction Reproduction Reproduction 7d Survival Rate Summary Conc-% Control Type 0 0 12.5 25 50 100 Conductivity C Lab Control Reproduction Summary Conc-% Control Type 0 0 12.5 25 50 100 Conductivity C Lab Control Count 10 10 10 10 10 10 Count 10 10 10 10 10 10 NOEL 100 50 Effect-% 50 Attribute Control Resp Control Resp Control Resp PMSD Mean 95% LCL 0.8 0.643 1 1 1 1 1 1 0.9 0.782 1 1 Mean 95% LCL 3.3 2.38 19.9 17.3 13.7 11 12.9 11.5 13.9 10.6 6.6 5.44 LOEL >100 100 Conc-% 74 Test Stat 1 19.9 19.9 0.317 95% UCL 0.957 1 1 1 1 1 95% UCL 4.22 22.5 16.4 14.3 17.2 7.76 TOEL N/A 70.7 95% LCL 25.8 PMSD N/A 31.7% 95% UCL 95.4 Acceptabilty Limits 0.8 - NL 15 -NL 15-NL 0.13-0.47 Min 0 1 1 1 0 1 Min 0 9 3 6 0 2 Max 1 1 1 1 1 1 Max 6 30 26 17 26 11 Method Fisher Exact/Bonferroni-Holm Test Dunnetf s Multiple Comparison Test Method Linear Interpolation Overlap Yes Yes Yes Yes Std Err 0.077 0 0 0 0.0577 0 Std Err 0.448 1.26 1.34 0.691 1.59 0.566 (ICPIN) Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Std Dev CV% 0.422 0 0 0 0.316 0 52.7% 0.0% 0.0% 0.0% 35.1% 0.0% Std Dev CV% 2.45 6.9 7.36 3.78 8.72 3.1 74.3% 34.7% 53.8% 29.3% 62.8% 46.9% Diff% 0.0% -25.0% -25.0% -25.0% -12.5% -25.0% Diff% 0.0% -503.0% -315.0% -291.0% -321.0% -100.0% 000-089-163-3 CETIS™ v1.6.3revE Analyst:: &S CETIS Summary Report Report Date: Link/Link Code: 29Oct-0817:17(p2of 2) 08-7802-0912/0809-S168 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 12.5 25 50 100 Reproduction Conc-% 0 0 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep 1 1 1 1 1 1 1 Rep1 0 22 3 6 6 9 Rep 2 1 t 1 1 1 1 Rep 2 6 13 13 8 5 2 Rep 3 1 1 1 1 1 1 Rep 3 6 24 16 14 16 11 Rep 4 0 1 1 1 1 1 Rep 4 0 21 14 17 7 6 RepS 1 1 1 1 1 1 RepS 3 23 4 16 22 7 Rep 6 1 1 1 1 1 1 Rep 6 4 9 26 12 17 3 Rep 7 1 1 1 1 1 1 Rep 7 5 10 21 16 21 4 RepS 1 1 1 1 0 1 RepS 5 25 9 14 0 10 RepS 0 1 1 1 1 1 Rep 9 0 30 20 16 26 9 Rep 10 1 1 1 1 1 1 Rep 10 4 22 11 10 19 5 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 15-3424-7594 Analyzed: 20 Oct-08 11:43 Data Transform Zeta Untransformed Fisher Exact/Bonferroni-Holm Test Control vs Conc-% Lab Control 12.5 25 50 100 Endpolnt: 7d Survival Rate Analysis: STP 2x2 Contingency Tables Alt Hyp C>T Test Slat 1 1 0.5 1 Data Summary Conc-% Control Type No-Resp Resp 0 Lab Control 10 12.5 10 25 10 50 9 100 10 7d Survival Rate Detail Conc-% Control Type Rep 1 0 Lab Control 1 12.5 1 25 1 50 1 100 1 Graphics l.O-i • • 0.9- a 0.8- | 0.7- | 0.6- . 3 0.5- S o.. 0.3- O.Z; 0.1- 0 0 0 1 0 Rep 2 1 1 1 1 1 • • 0 12.5 25 50 Conc-% Monte Carlo NOEL Not Run 100 P-Value Deeision(0.06) 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Total 10 10 10 10 10 Rep 3 Rep 4 Rep 5 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 • 100 Report Date: 29 Oct-08 1 7: 1 5 (p 1 of 1 ) Link/Link Code: 08-7802-09 12/0809-S 168 Nautilus Environmental (CA) CETIS Version: CETISvl.6.3 Official Results: Yes LOEL TOEL TU PMSD >100 N/A 1 N/A Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 1 1 11 1 1 1 11 1 11111 1 1 1 1 0 1 1 11 1 000-089-163-3 CETIS™ v1.6.3revE Analyst:^ CETIS Analytical Report Report Date: Link/Link Code: 290(51-08 17:15 (p1 of 2) 08-7802-0912/0809-S168 Ceriodaphnla 7-d Survival and Reproduction Test Analysis No: 10-8290-1386 Endpoint: Reproduction Analyzed: 29 Oct-08 17:14 Analysis: Parametric-Control vs Treatments Data Transform Zeta Alt Hyp Monte Carlo Untransformed C > T Not Run Ounnetf s Multiple Comparison Test Control vs Conc-% TestStat Critical MSD Lab Control 12.5 2.18 25* 2.46 50 2.11 100* 4.68 2.22 6.32 2.22 6.32 2.22 6.32 2.22 6.32 ANOVA Table Source Sum Squares Mean Square DF FStat Between 890.8 222.7 Error 1817.2 40.38222 Total 2708 263.0822 ANOVA Assumptions Attribute Test Variances Bartlett Equality of Variance Distribution Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count Mean 0 Lab Control 10 19.9 12.5 10 13.7 25 10 12.9 50 10 13.9 100 10 6.6 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 0 Lab Control 30 25 12.5 26 21 25 17 16 50 26 22 100 11 10 Graphics 30i i ; 25- | ] c I g ,.--..- __ |j 15- ^ j | 10- : ! ; 5- 4 5.51 45 49 TestStat Critical 11.9 13.3 0.974 95%LCL 95%UCL 17.3 22.5 10.9 16.5 11.5 14.3 10.6 17.2 5.42 7.78 Rep 3 Rep 4 24 23 20 16 16 16 21 19 9 9 "I j i " Reject Nul 0 12.5 25 50 100 Conc-% NOEL 50 P-Value 0.0545 0.0293 0.0631 0.0001 P-Value 0.0011 P-Value 0.0182 0.3450 Mln 9 3 6 0 2 Rep 5 22 14 14 17 7 15- 10- -5- •10- Nautilus Environmental (CA) CETIS Version: CETISvl 6.3 Official Results: Yes LOEL TOEL TU PMSO 100 70.7 2 31.7% Decision(5%) Non-Significant Effect Significant Effect Non-Significant Effect Significant Effect Decision(5%) Significant Effect Decision(1%) Equal Variances Normal Distribution Max StdErr Std Dev CV% 30 1.28 6.9 34.7% 26 1.37 7.36 53.8% 17 0.703 3.78 29.3% 26 1.62 8.72 62.8% 11 0.575 3.1 46.9% Rep 6 Rep 7 Rep 8 Rep 9 22 21 13 10 13 11 9 4 14 12 10 8 16 7 65 6543 * • • X ; ^ji* WV* .. f^ • .."•""' • -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Rankits Diff% 0.0% 31.2% 35.2% 30.2% 66.8% Rep 10 9 3 6 0 2 2.S 000-089-163-4 CETIS™ v1.6.3revE Analyst:, CETIS Analytical Report Report Date: Link/Link Code: 29 Oct-08 17:15 (p 1 of 1) 08-7802-0912/0809-S168 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-4057-6126 Endpoink Reproduction CETIS Version: CETISv1.6.3 Analyzed: 20 Oct-08 14:40 Analysis: Linear Interpolation (ICPIN) Official Results: Yes Linear interpolation Options X Transform Y Transform Seed Resamples Exp95%CL Method Linear Linear 7055475 280 Yes Two-Point Interpolation Point Estimates Effect-% Conc-% 95%LCL 95%UCL 50 74 . 25.8 95.4 Reproduction Summary Conc-% Control Type Count 0 Lab Control 10 12.5 10 25 10 50 10 100 10 Reproduction Detail Conc-% Control Type Rep 1 0 Lab Control 22 12.5 3 25 6 50 6 100 9 Graphics 20< 15- f 10- I 5- i . « • 0 20 40 60 Conc-% Calculated Variate Mean 19.9 13.7 12.2 13.9 6.6 Rep 2 13 13 8 5 2 80 Min Max 9 30 3 26 6 16 0 26 2 11 Rep 3 Rep 4 24 21 16 14 16 8 16 7 11 6 100 120 Std Err Std Dev CV% 1.26 6.9 34.7% 1.34 7.36 53.8% 0.719 3.94 32.3% 1.59 8.72 62.8% 0.566 3.1 46.9% Rep 5 Rep 6 Rep 7 23 9 10 . 4 26 21 16 12 16 22 17 21 734 Diff% 0.0% 31.2% 38.7% 30.2% 66.8% Rep 8 Rep 9 Rep 10 25 30 22 9 20 11 14 16 10 0 26 19 10 9 5 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 20 Oct-08 12:03 (p 1 of 2) 08-7802-0912/0809-S168 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 01-2141-7209 Endpoint: Reproduction Analyzed: 20 Oct-08 12:03 Analysis: Parametric-Two Sample CETIS Version: Official Results: QETISvl .6.3 Yes Comments: 'Shares Conductivity Control with Well 3. Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untran stormed C>T Not Run <0 N/A 20.2% Equal Variance t Two-Sample Test Control vs Control TestStat Critical USD P-Value Declslon(S%) Lab Control Conductivity Control 7.17 1.73 4.02 0.0000 Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(5%) Between Error 1377.8 483 1377.8 26.83333 1 18 51.3 0.0000 Significant Effect Total 1860.8 1404.633 19 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Decision(1%) Variances Variance Ratio F 7.93 Distribution Shapiro-Wilk Normality 0.923 6.54 0.0050 Unequal Variances 0.1160 Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Win Max Std Err Std Dev CV%Diff% Lab Control 10 19.9 17.3 22.5 9 30 1.28 6.9 34.7% 0.0% Conductivity Co 10 3.3 2.37 4.23 0 6 0.455 2.45 74.3% 83.4% Graphics 40-1 30- 20- 10- 0cc Conc-% -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 000-089-163-3 CETIS™ v1.6.3revE Analyst:. Freshwater Chronic Bioassay Client/Sample ID: Ductek & Associates / Well 1 Test No: 0809-S168 Cone. LC Rep 1 2 3 4 S 6 7 8 9 10 Analyst: Verified by; Board/ Cup ^ £*-jfH* Dally Reproduction/ Survival *h*:# 0 O 0 O 0 00 0 O O NO !!i^!!!!let OO O O 0 O<J O 0 V* ;*:**;O 0 0 0 0 O 0 O Ot} ^ !!iji#;!!i» O 5 5 O I "~5 «5 5 ^w ill!!!*!!:!! Oi- o• c?n C1 O• r\ \> n A & mm^ l ij'«*>n*i^^iIPvSTl> sr> SHIT:;;!;; »6-i3 Q.|6 <J Ib&SS 4$ iO ll_0 £S w$m * Total "isfe- gr-<•*£& «/(J5^1 10 QC ,0 H Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival !&!;!! <£> O OQ 00o 0\ <•1& iliilpii! ^**1 **^~ iii!!3ii;i:i ^•^ ^^^ !*!#j!i! ^ " HiliSiiili _^f^~ mm ^^^ %$m ^^ i!ii;8!j!!i ^^ Total U^*^ QC Cone. 12.5% Rep 1 2 3 4 S 6 7 8 9to ' 'Daily Reproduction/ Survival *!i!i 5t) 6 A63i~aooo illil^illilL> O Oooco•o 0u IjiilSilliio t? O 0 o 06 0 ^o Hip!I J 5 5 2> t b O 0 T) Illiiipill7-«;fp3"t \£7 ^Z1 |i|!!»i!|i!o O o 0\ /o>l CnA Y !i!il#!!iii Oq5 A,oo i~_\o a illliSii Total L^ii Vfr42^» Al "1io 1* QC -i, Time Fed/Test Solution Renewed (day): (0 Notes: Comments: QC Check: (1) \ t\5 (2) Daphnid Survival and Reproduction Datasheet Test Species: Qdubia^D. pulex D. magna Start Date/Time: 9/26/2008 I End Date/Time: 10^2008 (325 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival *•;;: 0a £? 0 0 00 H;i!&!;ij OO O O O O O Ofe O siinSjiii; O Oo o 0 0 0 0•o t? ^Hi4;;:;i 0 V Hna' 'L. ,4 .I5u iii'iSiiliil O 0 ^1 0 S} ^2, h 0 !!i=IS!!i!i <^L«k7 «.•? 1»nx7xfx\tf~kg} »5ft iliiilSfilili;r>o^2. «6 7 => 7 <f C> il]!!*!!!!! 4 ^ Total <^V '*?!,«^A |U V1-iv•HiUo QC -? Cone. 50% Rep 1 2 3 4 S 6 7 9 ^10 Daily Reproduction/ Survival 3!|i!in 6 <05 oO o #- £3 !i!i;«i;!iiooc?o oo o 0o P!!P!<£J oo O £) Oc? 0o 1:$!!!|a5 'i-V *b1 M 3o IlliiSliiiio^) O ^O po o "1 IliiiSli!!! y^G (o H-s ^ -^^'n Ljl- ii!ii?!iP!t f -5 i]_ i« ^l^O !$*$! • ._ Total Vs 1^7 "U-n ^1 ^W QC SB Cone. 100% Rep 1 2 3 4 S 6 7 8 9 10 Daily Reproduction/ Survival *!ii| c±o ooo 0 €> & 00 illilfcli'ioooo VOo op6 iiiiiiaiiilli D O £L Oooot> t> -5" !!i|i*i;ir 06 I O O $ftao ^s !!H«:i5!"+ n O5 -h O O t • C?" iiiiiSiiii; f^ r> ^ 6 b *?5 iiiiiiJiiiiii ft ~j L O "^ H•r S0 i!!S*|isl!Total 1-z. 11v7 •?,T \O4^* QC *• t /k3D (7)_ Neonates for each replicate were blocked across concentrations at test initiation; d = dead; M - male; LIP = lost in progress \ Nautilus Environmental 5550 Morehouse Drive, Suite 150. San D/ego, CV» 9212Y. Freshwater Chronic Bioassay Dudek & AssociatesClient: Sample ID: Test No: Well! 0809-S168 Concentration Day PH DO (mg/L) Cond. ((jmhos/cm) Temp CO PH DO (mg/L.) Temp CO Lab Control 2 I 3 h ft 23 26.0 24-0 5^5 -fo &£ Sl 6 IT Sfe" IS* 2-H.i, Concentration Da .aj^gag@$:;!sjsgisg^ PH DO (mg/L) Cond. ((imhos/cm) Temp (°C) PH DO(mfl/L) Temp ('C) 12.5% ffe^g.Ofe 74.0 4 6 7-7 s-os Animal Source/Date Received: Animal Age at Initiation: Sample Log-in Numbers: A: O £3~< [A/A C: B: Comments: QC Check: •K.i Water Quality Measurements Test Species: C. dubia Start Date/Time: 9/26/2008 End Date/Time: 10/2/2008 Concentration Day PH DO (mg/L) Cond. (pmhos/cm) Temp CO pH DO (mg/L) Temp (°C) 25% 1 I 2 | 3" T. 51 24- O lifo 24.0 6 I 7m. Rrtal 2,1. fe 7.7//o5 5-OZ Concentration Day PH DO (mg/L) Cond. (|imhos/cm) Temp (*C) PH DO (mg/L) Temp (°C) 50% 2S-8 7--OS S.4 2-4 - $?.*, •7.73 A5&sI*? rS.J <fs y>^ Concentration Da PH DO (mg/L) Cond. (Mmhos/cm) Temp ("C) PH DO (mg/L) Temp ('C) 100%..i r,,,,2 i » j g 4, 5W Ci-fo Vteiteal -i iaL 2^7.C.O 7- 23 4 Analysts: Initial: Final: Sample Used (A, B, C): MO A A /v A Final Review: Nautilus Environmental 5550 Morehouse Drive. Suite 150. San Diego, CA 92121. t i ' '? r Appendix B Sample Receipt Information Nautilus Environmental 5550 Morehouse Drive. Suite 150 San Diego, CA 92121 Client- Sample P:Golf Ge«r**- Tests Performed: Test ID No(s).: Sample (A, B.C) Log-in No. (08-xxxr) Sample Collection Date S Time fogftjfo (pHO Sample Receipt Dale & Time t/iffift Check-in Temperature (*C) Temperature OK? DO(mg/L) pH (units) Conductivity (uS/cm) Salinity (ppt; Alkalinity (mgrt^' Hardness (mg/L)- Total Chlorine (moA) Technician Initials i/aydg uoofo (n.O 32. )0 oe /o- o Jf. 2.- Co (3. 1). •=mgIL as CaCOj. *» Measured for freshwater samples only, NA = Not Applicable Culligan N = . ' Freshwater Te CortroVDflufon Water Source: Additional Control? Marine Tests: Control/Dilution Water Source: LABSW ARTSW Additional Control? Y N = Sample Salted w/ artificial salt? Y N Other Alkalinity: .Alkalinity:. Other Alkalinity:. Alkalinity.. Hardness: Hardness: Salinity:. Salinity: _ Sample salted w/orine? Y N If yes, what ppt?. If yes. what ppt?_ Sample Check-In Information Sample Description: COC Complete? A B C^ Filtration? Y Pore Size: Organisms ^_or Aeration? Y (itj Length of Time: Final DO: Final pH: pH Adjustment? Y (H Initial pH: Final pH: CtAdjustment? (Y )N Debris B Initial Free CV STS added: Final Free CI2: Sub-samples for additional chemistry: * / ii'<3 r\Jr Jj&si Comments: Temperature tor sample must be 0-6'C if received >24 hours past collection time. QC Check: Final Review: cfa Appendix C Chain-of-Custody Form OUHWii* D WASHINGTON n 8RmSH COLUMBIA S550Mi«hciu» Drive-Suite 150 5009 IMk Hipster -Suite 2 8664 Commerce Court SaiDiego,Coio™92121 lrara,Wa<tagron 98424 Bumaby British Columbia CaiwdoV5A4N7 Ptxine858.587.7333 Phone 253.922.4296 Phone 604.420.8773 fox 858.587.3961 Fox 253.922.581 4 Fox604.3S7.1361 Chain of Custody Date .Page.of ANALYSES REQUIRED Report to: Company _ Address 6Cfc~ Cityjg Contact Phone/Email SAMPLE ID ZT state #77 £5Z>7 DATE MATRIX Invoice to: Company Address City_ Contact Phone/Email State -Zip. CONMINEK TYPE NUMBER Of CONTAINERS COMMENTS uf ft Additional costs may be required for sample disposal or storage. Net 30 unless otherwise contracted. » '. < ' ' I'*'? DISTRIBUTION: WHITE • Nautilus Environmental, COLOR - Origmater January 26, 2009 EnviroMatrix I /Mv I Analytical, Inc. 26 January 2009 Dudek & Associates, Inc. EMA Log #: 0901355 Attn: Chris Oesch 605 Third Street Encinitas, CA 92024 Project Name: Carlsbad Golf Course Enclosed are the results of analyses for samples received by the laboratory on 01/15/09 15:08. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. I certify that this data is in compliance both technically and for completeness. Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewridge Avenue, Suite A - San Diego, California 92123 - (858) 560-7717 - Fax (858) 560-7763 Analytical Chemistry Laboratory Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EM A Log #: 0901355 ANALYTICAL REPORT FOR SAMPLES Sample ID Laboratory ID Matrix Date Sampled Date Received DOWNSTREAM UPSTREAM WELLS WELL1 0901355-01 0901355-02 0901355-03 0901355-04 Water Water Water Water 01/15/0909:45 01/15/09 10:05 01/15/09 10:30 01/15/09 10:45 01/15/09 15:08 01/15/09 15:08 01/15/09 15:08 01/15/09 15:08 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix Page 2 of7 Client Name: Dudek& Associates, Inc. Project Name: Carlsbad Golf Course EMALog#:0901355 Conventional Chemistry Parameters by Standard/EPA Methods Analyte DOWNSTREAM (0901355-01) Water Dissolved Oxygen pH Phosphorus, Total Total Suspended Solids Result Sampled: 7.60 7.51 0.08 ND ReportingLimit Units 01/15/0909:45 Received: 0.10 0.10 0.05 20 mg/1 pH Units mg/1 Dilution Batch Prepared Analyzed Method Notes 01/15/09 15:08 1 N N 9012232 901 1601 9011618 9012121 01/15/09 01/1 5/09 01/16/09 01/21/09 01/15/09 01/15/09 01/16/09 01/22/09 SM4500-OG SM4500-H+B SM4500PB.E SM2540D HT-15 HT-15 UPSTREAM (0901355-02) Water Sampled: 01/15/09 10:05 Received: 01/15/09 15:08 Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids WELL 3 (0901355-03) Water Sampled Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids WELL 1 (0901355-04) Water Sampled Dissolved Oxygen PH Phosphorus, Total Total Suspended Solids 7.97 7.66 0.06 ND : 01/15/09 2.80 6.63 0.78 807 : 01/15/09 2.81 5.90 0.23 27 0.10 0.10 0.05 20 10:30 Received 0.10 0.10 0.05 20 10:45 Received 0.10 0.10 0.05 20 mg/1 pH Units mg/1 1 (I 9012232 901 1601 9011618 9012121 01/15/09 01/15/09 01/16/09 01/21/09 01/15/09 01/15/09 01/16/09 01/22/09 SM4500-OG SM4500-H+ B SM4500PB.E SM2540D HT-15 HT-1S : 01/15/09 15:08 mg/1 pH Units mg/1 : 01/15/09 mg/1 pH Units mg/I 1 15:08 1 N H 9012232 9011601 9011618 9012121 9012232 9011601 9011618 9012122 01/15/09 01/15/09 01/16/09 01/21/09 01/15/09 01/15/09 Oi/16/09 01/21/09 01/15/09 01/15/09 01/16/09 01/22/09 01/15/09 01/15/09 01/16/09 01/22/09 SM4500-OG SM4500-H+ B SM4500PB,E SM2540D SM4500-OG SM4500-H+ B SM4500PB.E SM2540D HT-15 HT-15 HT-15 HT-15 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix Analytical, Inc. Page 3 of 7 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EMALog#: 0901355 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Reporting Spike Source %REC RPD Result Limit Units Level Result %REC Limits RPD Limit Notes Batch 9011601 Duplicate (9011601-DUP1)Source: 0901335-01 Prepared & Analyzed: 01/15/09 PH Reference (9011601-SRMI) 8.67 0.10 pH Units 8.70 Prepared & Analyzed: 01/15/09 0.3 Total Suspended Solids ND 20 mg/1 20 PH Batch 9011618 Blank (9011618-BLK1) Phosphorus, Total LCS(9011618-BS1) Phosphorus, Total LCS Dup (9011618-BSD1) Phosphorus, Total Duplicate (9011618-DUP1) Phosphorus, Total Matrix Spike (9011618-MS1) Phosphorus, Total Matrix Spike Dup (9011618-MSD1) Phosphorus, Total Batch 9012121 Blank (9012121-BLK1) 8.98 ND 0.51 0.51 0.12 0.60 0.60 0.10 0.05 0.05 pH Units mg/1 mg/1 0.05 mg/1 Source: 0901177-01 0.05 mg/1 Source: 0901177-01 0.05 mg/1 Source: 0901177-01 0.05 mg/1 9.10 99 97-102.96 Prepared & Analyzed: 01/16/09 Prepared & Analyzed: 01/16/09 0.500 102 80-120 Prepared & Analyzed: 01/16/09 0.500 102 80-120 0.4 Prepared & Analyzed: 01/16/09 0.11 13 Prepared & Analyzed: 01/16/09 0.500 0.11 98 80-120 Prepared & Analyzed: 01/16/09 0.500 0.11 99 80-120 0.2 Prepared: 01/21/09 Analyzed: 01/22/09 20 20 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. • EnviroMatrix [ ,-Mtv JAnarytical, Inc. Page 4 of7 Client Name: Dudek & Associates, Inc. ProjectName: Carlsbad Golf Couise Conventional Chemistry Anatyte Result EMALog#: 0901355 Parameters by Standard/EPA Methods - Quality Control Repotting Limit Units Spike Source %REC RPD Level Result %REC Limits RPD Limit Notes Batch 9012121 Duplicate (9012121-DUP1) Total Suspended Solids 3.0 Reference (9012121-SRM1) Total Suspended Solids 62.3 Batch 9012122 Blank (9012122-BLK1) Total Suspended Solids ND Duplicate (9012122-DUP1) Total Suspended Solids 26.0 Reference (9012122-SRM1) Total Suspended Solids 60.9 Batch 9012232 Duplicate (9012232-DUP1) Dissolved Oxygen 7.52 Duplicate (9012232-DUP2) Dissolved Oxygen 8.59 Duplicate (9012232-DUP3) Source: 0901355-01 20 mg/1 20 mg/1 20 mg/1 Source: 0901355-04 20 mg/1 20 mg/1 Source: 0901355-01 0.10 mg/1 Source: 0901355-02 0.10 mg/1 Source: 0901355-03 Prepared: 01/21/09 Analyzed: 01/22/09 9.0 100 20 QR-04 Prepared: 01/21/09 Analyzed: 01/22/09 60.6 103 1.51-107.2' Prepared: 01/21/09 Analyzed: 01/22/09 Prepared: 01/21/09 Analyzed: 01/22/09 27.0 4 20 Prepared: 01/21/09 Analyzed: 01/22/09 60.6 100 1.51-107.2' Prepared & Analyzed: 01/15/09 7.60 1 20 Prepared & Analyzed: 01/15/09 7.97 7 20 Prepared & Analyzed: 01/15/09 Dissolved Oxygen 2.73 0.10 mg/1 2.80 3 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. - EnviroMatrix I M\ 1 Analytical, Inc. Page 5 of7 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad Golf Course EM A Log #: 0901355 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Reporting Result Limit Units Spike Level Source Result %REC RPD %REC Limits RPD Limit Notes Batch 9012232 Duplicate (9012232-DUP4)Source: 0901355-04 Prepared & Analyzed: 01/15/09 Dissolved Oxygen 3.06 0.10 mg/I 2.81 9 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in Us entirety. -EnviroMatrixf 7* J Analytical, Inc. Page 6 of7 Client Name: Dudek & Associates, Inc. EMALog#: 0901355 Project Name: Carlsbad Golf Course Notes and Definitions QR-04 The RPD between the sample and sample duplicate is not valid since both results are below the reporting limit for this analyte. HT-15 This sample was received outside of the EPA's recommended 15 minute holding time for this analysis. However, the sample was analyzed immediately upon receipt. ND Analyte NOT DETECTED at or abo\e the reporting limit NR Not Reported dry Sample results reported on a dry weight basis RPD Relative Percent Difference The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. - EnviroMatrix [ Mv ] Analytical, Inc. Page 7 of7 CHAIN-OF-CUSTODY RECORD EnviroMatrix[™^ Analytical,Inc. 4340 Viewridge Ave., Ste. A - San Diego, CA 92123 - Phone (858) 560-7717 - Fax (858) 560-7763 Requested Analysis Page of. Phone:F«X. Email: Billinfi Address: PioieotID: PO*: ID*Client Sample ID Sample Date San^ile Time Sample Matrix Container #/Type 664* 10 MrtrtxCMo: A - Air, DW-Drinking %ter, OW-Orotmdvwtcr, SW-Storm W.ter WW°Wt?tew«rer,5-Soll,SEP"SadimOTt,SP°Solid,T-'nMue,O-Oil.L-Liqnid REL..INQUIRED BY DATE/TIME RECEIVED I SMpped^fi oCourier oUFS a FedEx olISPS a Client Drop Off aOflur aSameDiy a24hr a4lhr a3ihy D4d»y a5d*y oRpt oPDF o Excel o Oeoft»ckef/EDF Q Hard Copy DEPT oByljbonrtory o'Return to Client: P/UtrDelivgy D Archive Compmy: ConwtContainerj.- Yes No N/A CooUinenProptrlyPreseved: Yes No N/A Custody Seilj Intact Yes No WA COCVUbelsAgree: Yes No N/A Signature SigMtBt TtapgRocapl:Print ftint Smpled By: Otent EMA AuKuampler Company:Cornpny: 'Addidon4loortm«y«pply,eon8oM«prq)ectnMi«t«rfordeWli. While-EMA 'EMA reserves the right to return «ny«mplesltat do not rastch our vraste profile. NOTE: ByretbKruiJhingampl«itoEMA,^cHait<sf<^topvforllKKrvi(»reqtu^ of ifier 30 dtysonlsM otherwise noted. AllwotkisiubieclioFMA'.« •• Canary-Accounting Pink-dicnt(Vrepofl)OoW. Client (Rdinqufsh) California 5550 Morehouse Drive Suite 150 San Diego, CA 92121 85&587-T333 fax:858-587-6769 Washington 5009 Pacific Highway East Suite 2 TacomaWA 98424 253-922-4296 fax-253-922-5814 British Columbia 8664 Commerce Court Bumaby, British Columbia V5A4N7 604-603-938} fox-604^03-9381 Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Testing Period: January 2009 Prepared for: Testing Location: Submitted: Dudek & Associates, Inc. 605 Third Street Encinitas, CA 92024 Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 March 5, 2009 Data Quality Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified Date: TOXICITY SUMMARY REPORT Test ID Number: 0901-S032 to -S035 Client: Dudek & Associates Monitoring Period: January 2009 Sample Information Client: Dudek & Associates, Inc. Project Study: Carlsbad Golf Course Toxicity Evaluation - January 2009 Sample Site lO's: a) Downstream b) Upstream c) Well #3 d) Well #1 Sample Collection Date, Time: 1/15/09, 09:45 to 10:45 Sample Receipt Date, Time: 1/16/09,10:10 Sample Material; Grab samples; groundwater and site runoff Chronic Toxlcltv Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Control: Protocol Used: Test Acceptability Criteria: 1/16/09 to 1/23/09 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, < 24 hours 100, 50, 25,12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity = 200 uS/cm Lab freshwater spiked with seawater to raise the conductivity to match the sample's conductivity levels, which roughly ranged from 3,000 to 5,000 uS/cm. This control was conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Table 1. Water Quality Information: Samples Upon Receipt Sample ID Downstream Upstream Well #3 Well #1 pH 7.80 7.79 7.34 7.26 DO (mg/L) 9.8 10.6 5.4 5.7 Temp <°C) 11.2 11.2 11.2 11.2 Conductivity 1 (u,S/cm) 3100 3510 4320 2670 Hardness (mg/L as CaCOj) >1000 >1000 >1000 >1000 Alkalinity (mg/L as CaCO3) 255 197 127 41 1 The conductivity meter was not measuring accurately at the time of sample receipt (temperature adjustment factor not working). Actual conductivity levels of each sample were measuring approximately 500 uS/cm higher at test initiation. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Test ID Number: 0901-5032 to -S035 Client: Dudek & Associates Monitoring Period: January 2009 Table 2. Summary of Test Results Sample ID Concentration (%) Lab Control Downstream 6.25 12.5 25 50 100 Cond. Control Lab Control Upstream 6.25 12.5 25 50 100 Cond. Control Lab Control Well #3 6.25 12.5 25 50 100 Cond. Control Lab Control Well #1 6.25 12.5 25 50 100 Cond. Control Mean Percent Survival 100 100 100 100 100 100 100 100 100 100 90 100 100 90 100 100 100 100 90 11 * 60* 100 100 90 100 100 90 100 Mean Number of Offspring Produced 24.2 24.9 26.3 28.4 25.0 16.1 * 17.3* 24.9 25.6 28.6 25.3 20.5 14.7* 11.6* 26.1 22.8 20.2 19.5* 9.5* 0.0 * 3.9* 26.0 24.8 24.7 26.4 23.4 0.0* 17.1 * * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Note: The Conductivity Control was lab water spiked to match the conductivity of the 100% sample, and was used as a comparison to determine whether conductivity levels had an effect on the final results. The approximate conductivity levels of each sample were as follows: 3700 pS/cm (Downstream); 4400 uS/cm (Upstream); 4900 uS/cm (Well #3); and 3200 uS/cm (Well #1). Nautilus Environmental - San Diego Bioassay Laboratory TOX1CITY SUMMARY REPORT Client: Dudek & Associates Test ID Number: 0901-S032 to -S035 Monitoring Period: January 2009 Table 3. Summary of Statistical Results and Reportable Values Sample ID Test & Endpoint Downstream: Survival Reproduction Upstream: Survival Reproduction Well #3: Survival Reproduction Well#1: Survival Reproduction NOEC (% effluent) 100 50 100 50 50 12.5 100 50 ECso (% effluent) >100 >100 >100 >100 71.1 41.1 >100 72.2 TUc (toxicity units) 1.0 2.0 1.0 2.0 2.0 8.0 1.0 2.0 NOEC - the highest Concentration that produces No Observed Effect ECso = concentration that causes a 50% adverse effect to the test organisms Chronic Toxicity Units (TUc) = 100 r NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. There was no toxicity observed to the survival endpoint with three of the sample sites. However, the Well #3 sample did show toxicity to survival at the 100% concentration. More of an effect was observed with the reproduction endpoint. The same three sample sites showed a decrease in reproduction (i.e. toxicity) at the 100% concentration, but the Well #3 sample showed a decrease in reproduction down to the 25% concentration. The adverse effects to reproduction were expected however, due to the elevated levels of conductivity found in the samples. From internal studies and experience, we have consistently seen adverse effects to the reproduction endpoint when conductivity levels exceed approximately 2,500 uS/cm. Conductivity is related to the concentration of salts in a sample. The optimum range for Ceriodaphnia is between approximately 100 and 1,500 uS/cm. The correlation between .higher conductivity levels and decreased reproduction in Ceriodaphnia was observed in this study as well. Since all four samples had conductivity levels greater than 2,500 uS/cm (ranging from 3,000 to 5,000 uS/cm), an adverse effect to the reproduction was expected. The Well #3 sample had the highest conductivity level (approximately 4,900 uS/cm), and as expected, showed the greatest decrease in the number of offspring produced. The 100% concentration of Well #3 resulted in 0% reproduction. The Conductivity Controls for both the Upstream and Downstream samples showed a similar response with the reproduction endpoint. This would seem to indicate that conductivity levels were the primary driver to toxicity with these samples. The Well #3 and Well #1 samples, on the other hand, showed a greater adverse effect compared to the conductivity controls. This would seem to indicate that, in addition to the high conductivity levels, there might have been another factor involved that contributed to the toxicity. Nautilus Environmental - San Diego Bioassay Laboratory Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis Site: Downstream CETIS Summary Report Report Date: Link/Link Code: 05Mar-0911:50(p1 of 2) 21 -0460-7128J0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: Start Date: Ending Date: Duration: Sample No: Sample Date: Receive Date Sample Age: 10-1066-9411 Test Type: Reproduction-Survival (7d) 16 Jan-09 13:20 Protocol: EPA/821/R-02-013 (2002) 23 Jan-09 1 1 :35 Species: Ceriodaphnia dubia 6d 22h Source: In-House Culture 16-9753-5634 Code: 09-0029 15 Jan-09 09:45 Material: Downstream Ambient Sample 1 6 Jan-09 10: 1 0 Source: Dudek & Associates 28h(11.2'C) Station: Downstream Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client: Dudek & Associates Project: Comparison Summary Analysis No 18-3519-7903 18-2278-2472 Endpoint 7d Survival Rate Reproduction NOEL 100 50 LOEL >100 100 TOEL N/A 70.7 PMSD N/A 19.8% Method Fisher Exact/Bonferroni-Holm Test Bonferroni Adj t Test Point Estimate Summary Analysis No 10-8405-1988 Endpoint Reproduction Effect-% 50 Conc-% >100 95% LCL N/A 95% UCL N/A Method Linear Interpolation (ICPIN) Test Acceptability Analysis No 18-3519-7903 10-8405-1986 18-2278-2472 18-2278-2472 Endpoint Attribute 7d Survival Rate Reproduction Reproduction Reproduction Control Resp Control Resp Control Resp PMSD TestStat 1 24.2 24.2 0.198 Acceptably Limits 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Overlap Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 8 Mean 1 1 1 1 1 1 1 95% LCL 1 1 1 1 1 1 1 95% UCL 1 1 1 1 1 1 1 Min 1 1 1 1 1 1 1 Max 1 1 1 1 1 1 1 StdErr 0 0 0 0 0 0 0 Std Dev 0 0 0 0 0 0 0 CV% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% Diff% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 8 Mean 17.3 24.2 24.9 26.3 28.4 25 16.1 95% LCL 15.4 22.3 23.1 25.2 27.3 23.6 14.1 95% UCL 19.2 26.1 26.7 27.4 29.5 26.4 18.1 Min 9 11 14 22 24 18 7 Max 25 29 32 32 33 31 24 Std Err 0.923 0.926 0.867 0.558 0.539 0.672 0.988 Std Dev 5.06 5.07 4.75 3.06 2.95 3.68 5.41 cv% 29.2% 21.0% 19.1% 11.6% 10.4% 14.7% 33.6% Drff% 0.0% -39.9% -43.9% -52.0% -64.2% ^4.5% 6.79% 000-089-163-3 CETIS™ v1.6.3revE Analyst:.QA:f CETIS Summary Report Report Date: Link/Link Code: 05Mar-0911:50(p2of 2) 21 -0460-7128/0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 1 Rep1 11 26 29 32 27 27 11 Rep 2 1 1 1 1 1 1 1 Rep 2 18 25 25 26 24 25 18 Rep 3 1 1 1 1 1 1 1 Rep 3 18 22 23 27 31 23 17 Rep 4 1 1 1 1 1 1 1 Rep 4 25 28 32 29 28 24 17 Rep 5 1 1 1 1 1 1 1 Rep 5 13 27 27 27 28 23 24 Rep 6 1 1 1 1 1 1 1 Rep 6 17 24 27 26 24 27 14 Rep 7 1 1 1 1 1 1 Rep 7 23 29 25 23 33 18 Rep 8 1 1 1 1 1 1 1 Rep 8 9 24 14 22 30 31 7 Rep 9 1 1 1 1 1 1 1 Rep 9 19 26 24 23 28 23 21 Rep 10 1 1 1 1 1 1 Rep 10 20 11 23 28 31 29 000-089-163-3 CETIS ™v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Data: Link/Link Code: 05Mar-0911:50(p1 of 1) 21-0460-7128/0901-S035 Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 18-3519-7903 Endpoint: 7d Survival Rate Analyzed: 13 Feb-09 13:15 Analysis: STP 2x2 Contingency Tables CETIS Version: Official Results: CETISV1.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C>T Not Run 100 >100 N/A N/A Fisher Exact/Bonferronl-Holm Test Control vs Conc-%TestStat P-Value Decision(O.OS) Lab Control 6.25 12.5 25 50 100 1 1 Non-Significant Effect 1 1 Non-Significant Effect 1 1 Non-Significant Effect 1 1 Non-Significant Effect 1 1 Non-Significant Effect Data Summary Conc-% Control Type No-Rasp Resp Total 0 6.25 12.5 25 50 100 Lab Control 10 10 10 10 10 8 0 0 0 0 0 0 10 10 10 10 10 8 7d Survival Rate Detail Conc-% Control Type Repl Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control Graphics i.o- O.fr 0.8- 0.7- O.fr O.S- 0.+ 0.3 0.2- 0.1- 0.0 6.25 1Z.5 25 Conc-% 100 000-089-163-4 CETIS™ v1.6.3revE Analyst,QA: CETIS Analytical Report Report Date: Link/Link Code: 05 Mar-09 11:50 (p 1 of 2) 21 -0460-7128/0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 18-2278-2472 05 Mar-09 11:47 Data Transform Zeta Untransformed Bonferroni Adj t Test Control vs Conc-% Lab Control ANOVA Table Source Between Error Total 6.25 12.5 25 50 100* Sum Squares 750.3491 923.875 1674.224 Endpoint Reproduction Analysis: Parametric-Multiple Comparison Alt Hyp C>T Test Stat -0.371 -1.11 -2.23 -0.424 4.04 Mean Square 150.0698 17.76683 167.8367 ANOVA Assumptions Attribute Test Variances Distribution Bartlett Equality of Variance Shapiro-Wilk Normality Reproduction Summary Conc-% Control Type Count 0 6.25 12.5 25 50 100 Lab Control 10 10 10 10 10 8 Mean 24.2 24.9 26.3 28.4 25 16.1 Monte Carlo Not Run Critical 2.4 2.4 2.4 2.4 2.4 DF 5 52 57 Test Stat 5.42 0.946 95% LCL 22.3 23.1 25.1 27.3 23.6 14.1 MSO 4.52 4.52 4.52 4.52 4.8 FStat 8.45 Critical 15.1 95% UCL 26.1 26.7 27.5 29.5 26.4 18.2 NOEL 50 P-Value 1.0000 1.0000 1.0000 1.0000 0.0004 P-Value 0.0000 P-Value 0.3670 0.0126 Min 11 14 22 24 18 7 Nautilus Environmental (CA) CETIS Version: CETISvl.6.3 Official Results: Yes LOEL TOEL TU PMSO 100 70.7 2 19.8% Decision(S%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision(5%) Significant Effect Decision(1%) Equal Variances Normal Distribution Max StdErr StdDev CV% 29 0.942 5.07 21.0% 32 0.882 4.75 19.1% 32 0.568 3.06 11.6% 33 0.548 2.95 10.4% 31 0.684 3.68 14.7% 24 1 5.41 33.6% Diff% 0.0% -2.89% -8.68% -17.4% -3.31% 33.4% 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report ReP°rt Date: Link/Link Code: 05Mar-0911:50(p2of 2) 21-0460-7128/0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 18-2278-2472 Endpoint Reproduction Analyzed: 05 Mar-09 11:47 Analysis: Parametric-Multiple Comparison CETIS Version: CETISvl .6.3 Official Results: Yes Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 6.25 12.5 25 50 100 Lab Control 29 32 32 33 31 24 28 29 29 31 29 21 27 27 28 31 27 18 26 27 27 30 27 17 26 25 27 28 25 17 25 25 26 28 24 14 24 24 26 28 23 11 24 23 23 27 23 7 22 23 23 24 23 11 14 22 24 18 Graphics 40-1 *" 30- 10- 10- Reject NUU -10- 1 1 r~ i 1 0 6.25 12.5 25 50 100 Conc-% -15-T [ 1 1 l~~~—I [ 1 12.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Ranklts 000-089-163-4 CETIS™ v1.6.3revE Analyst:: El CETIS Analytical Report Report Date: Link/Link Code: 05Mar-09 11:50(p1 of 1) 21 -0460-7128/0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No Analyzed: 10-8405-1988 Endpoint Reproduction 05 Mar-09 1 1 :47 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Resamptes Linear Linear 7055475 280 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes Exp 95% CL Method Yes Two-Point Interpolation Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 >100 N/A N/A Reproduction Summary Conc-% 0 6.25 12.5 25 50 100 Control Type Count Lab Control 10 10 10 10 10 8 Mean 24.2 24.9 26.3 28.4 25 16.1 Min 11 14 22 24 18 7 Calculated Variate Max 29 32 32 33 31 24 StdErr 0.926 0.867 0.558 0.539 0.672 0.988 Std Dev 5.07 4 75 3.06 2 95 3.68 5.41 CV% 21.0% 19.1% 11.6% 10.4% 14.7% 33.6% Diff% 0.0% -2.89% -8.68% -17.4% -3.31% 33.4% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 30- *, § 20" 1 ^ 15- 10- 5- Control Type Rep 1 Lab Control 26 29 32 27 27 11 • • ' ' • 0 20 40 60 Conc-% Rep 2 25 25 26 24 25 18 80 Rep 3 22 23 27 31 23 17 * 100 120 Rep 4 28 32 29 28 24 17 Rep 5 27 27 27 28 23 24 Rep 6 24 27 26 24 27 14 Rep 7 29 25 23 33 18 7 Rep 8 Rep 9 24 26 14 24 22 23 30 28 31 23 21 Rep 10 11 23 28 31 29 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report r<l/f. Report Date: Link/Link Code: 05Mar-0911:52(p1 of 1} 21 -0460-7128/0901-S035 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 06-9177-6537 Analyzed: 05 Mar-09 11:48 Endpoint: Reproduction Analysis: Parametric-Two Sample CETIS Version: CETISv1.6.3 Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed OT Not Run <0 N/A 16.2% Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Peci8ion(5%) Lab Control Conductivity Control 3.05 1.73 3.93 0.0035 Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Declsion(5%) Between Error 238.05 461.7 238.05 25.65 1 18 9.28 0.0069 Significant Effect Total 699.75 263.7 19 ANOVA Assumptions Attribute Test TestStat Critical P-Value Decislon(1%) Variances Variance Ratio F 1.01 Distribution Shapiro-Wilk Normality 0.915 6.54 0.9920 Equal Variances 0.0802 Normal Distribution Reproduction Summary Conc-% Control Type Count Mean 95%LCL 95%UCL Win Max Std Err Std Dev CV%Dirf% Lab Control 10 24.2 22.3 26.1 11 29 0.942 5.07 21.0% 0.0% Conductivity Co 10 17.3 15.4 19.2 9 25 0.939 5.06 29.2% 28.5% Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 Conductivity C 25 23 20 19 18 18 17 13 11 9 Lab Control 29 28 27 26 26 25 24 24 22 11 Graphics 30-| 25- 20- 15- 10- 10-1 5- Rejfect Null -5- -10- -is- Conc-%L.C, -2.0 -1.5 -1.0 -OS 0.0 0.5 1.0 1.S 2.0 Ranktts 000-089-163-4 CETIS™ v1.6.3revE Analyst. =reshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna mple ID:Dudek & Associates - Downstream Start Date/Time: 1 /1 6/2009 \ 3 2.^ dumber: C0ni-5C>?6 End Date/Time: 1/23/2009 u^S Cone. LC Rep 1 2 3 4 5 Q 7 8 10 Analyst Verified by: Board/ Cup •2. V,t q 5 (, d |3 jj V4> »7I fl1 1 Z.O ES 36, Daily Reproduction/ Survival #1:!$:!:!:! b 0 Q 0 0 0 0 Q/a 6 il& j:|:j:jZ|ii:li O 0oo•no 0o V Y& ;:::|:;3t;JH;! f) YQ 3(. 0 flo ~i —r^ j;::j::Si:j:!: 3 <3 6 ^~T — ^L liiiilSiiiii!& o I ^ft O O O "DP fc* « W r _13>L7 au/ j&f-- :;j;:;;Z;i;i;i; \'^ ^ \ \\ 1 il^C \^i^ ^ y PA/*T |:j:ii^ij;ii; £? Total ^2^*21/5 5^*^^ *3S^-j ^if •5/5 -^ i«tV.' H QC n w (4) > 5 CO (5)(7). Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival ' 1 Ml;! £ 0n 00 0t)oo K mwm 0 0nn Q0o 0o-o ;iH:!;5;!i!Hng3(7 Q0 0 0aI? !ii!i!i*^M O 3 1. ^a 3j, Q 3 3 xmmIP^ Q ^b 0oo 0(\ i^:::S;:::;!: /'4*r7tf H*r^v4 ;«:7:i;ii;; 6> (.3 <?n 7 ^\\ «r 10 ti ;!:h!ffii!H;i Total K ^^^"2> 11 a3> 1 IT -2O Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Dally Reproduction/ Survival " 1 ii (•)& : 0n Q0 Q,0o 0 !!!ii;iiS!!i!ii O O Oo O O Oo 0u :i:l:!;5:!Hi; 0 O 0 ? C7 O 0 (20 0 !*!;!4!:!# 4 ^5 <? 5£s"613 mmm6 o O1,3. O O ID O ISL c> !!!!!!«!!!!!!! 10 ^Mr('LJ^'2^c>^ o"I :;;;:;7;:^;:; »^ l"2"ix O \-L>\^y«• ^H j!ii!ii«ili!!!i Total "^•*1 2S^.2, 2^$7a-i «^**_^ »^aw^^, Cone. 12.50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival'y.1 &b ^/5 (7 t> Q ffd 0 A I!!*!?;*!; Q5o 0 0 0 ^. t5ffl ^ jl!i;!l5!i!liln O O r3 <7 0 /9 O 50 yymm MH<3^•^H S1 « c\ t\mmo O 'O <UO p5Vo 6 !!!!!!!S!!!!l!l \3r-7a ItT ^t^C? O ^A |i!;;:17:i:!:|:tu»c»"i.» \^\^ ip<?{ LO is i!!l!!Sll!!|!Total ^»P. 30- 3-1 3^-1 ?T9k (j» Q^>:*£*.a^>»e> QC «r P» QC y /»« QC (? M* Time Fed/Test Solution Renewed (day): 0) V5Z.<5 f 1) 1 6£^> (2) g^^7 (3)_U Notes: d = dead; M ° male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) Comments: _ __ _ _ QC Check: fc/L Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates - Downstream Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dub/a D. pu/ex D. magna Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Cone.Rep Board/ Cup Daily Reproduction/ Survivalmmmmmm Total QC o JA25%0 V & V 0 l\0 o.4- * 0 o 2Huo600 0 0 0 10 IS.0 u to Analyst Es Verified by:CT6 Cone.Rep Daily Reproduction/ Survival Total i QC 50%0 -o D :0 o IDoooo * o o o Vb 10 r\ Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survivalmm 00o A66 UP&o L?P [Iliii^jlii T> O O O o O' -- O O — ;i:iii$i!i!i!i 0 0 0^ d 0_ d 0 *^- liiiliitHBj! 3 ^t/o 3 2— • 3 M :!!$B(i!!t!j © O 0 5 €> O — _^L rP — f =jlji| 0. g1^10 J,l$—-H-7_/ !!![i!3l!!iil! 0 & C O \0q — o tO — !i!!!H«!5!!!5 — • — - Total 1) 1& 4-1 •1 ?*• W MP 1 ^-\ UP QC ^ w Time Fed/Test Solution Renewed (day): Notes: d a dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) Comments: QC Check:Final Review: / Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client: Dudek & Associates Water Quality Measurements Test Species: C. dub/a Sample ID: Downstream Start Date/Time: 1/16/2009 TestNo: OTOJ-5635 End Date/Time: 1/23/2009 Concentration Da Conductivity Control 6 •"'MM. - r...._ -2.4-H Concentration Day PH DO (mg/L) Cond. ((jmhos/cm) Temp CC) PH DO (mg/L) Temp CO 6.25% S-O3 7-2 SO Z^-l^-.A'.-.tepft--'* ZM-"ZS.S"as-. • 8-3 •7.17 Concentration Day 12.5% ?l * PH DO (mg/L) Cond. (pmhos/cm) Temp (°Cl 1& PH DO (mg/L) Temp CO s-n 2.S1 U.3L Analysts: Initial: Final: Sample Used (A, B, C): Comments: MD JG A PH? 4 au DP A Df A H Animal Source/Date Received: THtl/T?^/ /N f* Sample Log-In Numbers: A: CA|--C(jl^^1 B: QC Check:L_ Animal Age at Initiation: _ C: Nautilus Environmental 5550 Moreho liua •ehouse Drive, Final Review: Suite 150. San D/ego, C/l 92f 21. Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek & Associates Sample ID: Downstream Test No: Test Species: C. dub/a Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Concentration Day PH 00 (mart.) Cond. (pmhos/cm) Temp ("C)m 25% Tfa -i- a. 15-0 K-O JJ.03- PH DO (mg/L) Temp CO Concentration Da 8.1. ZV.f, 2H.9 50% PH DO (mg/L) Cond. (iimhos/cm) Temp (*C PH DO (mg/U Temp ("C) TS7 T^TS 1^0 11-70 % 5050 . [0 8.31 Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: Sample Log-in Numbers: A: QC Check: Nfuttha environmental SS50 Honhousa Drive, Softs f SO. San Diego, CA 92721. Site: Upstream CETIS Summary Report Report Date: Link/Link Code: 13Feb-0913:12(p1 of 2} 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: Start Date: Ending Date: Duration: Sample No: Sample Date: Receive Date Sample Age: 14-8678-8906 Test Type: Reproduction-Survival (7d) 16 Jan-09 13:20 Protocol: EPA/821/R-02-013 (2002) 23 Jan-09 1 1 :05 Species: Ceriodaphnia dubia 6d 22h Source: In-House Culture 18-5185-4435 Code: 09-0030 15 Jan-09 10:05 Material: POTW Effluent 16 Jan-09 10:10 Source: Dudek & Associates 27h(11.2*C) Station: Upstream Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client: Dudek & Associates Project: Comparison Summary Analysis No 03-8037-1926 06-0511-0252 End point 7d Survival Rate Reproduction NOEL 100 50 LOEL >100 100 TOEL N/A 70.7 PMSD N/A 29.8% Method Fisher Exact/Bonferroni-Holm Test Wilcoxon/Bonferroni Ad] Test Point Estimate Summary Analysis No 19-7163-7563 Endpoint Reproduction Effect-% 50 Conc-% >100 95% LCL N/A 95% UCL N/A Method Linear Interpolation (ICPIN) Test Acceptability Analysis No 03-8037-1926 06-0511-0252 19-7163-7563 06-0511-0252 Endpoint Attribute 7d Survival Rate Reproduction Reproduction Reproduction Control Resp Control Control PMSD Resp Resp Test Stat 1 24.9 24.9 0.298 Acceptab 0.8 - NL 15 -NL 15 -NL Ity Limits 0.13-0.47 Overlap Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 9 Mean 0.9 1 1 1 0.9 1 1 95% LCL 0.782 1 1 1 0.782 1 1 95% UCL 1 1 1 1 1 1 1 Min 0 1 1 1 0 1 1 Max 1 1 1 1 1 1 1 Std Err 0.0577 0 0 0 0.0577 0 0 Std Dev 0.316 0 0 0 0.316 0 0 CV% 35.1% 0.0% 0.0% 0.0% 35.1% 0.0% 0.0% Diff% 0.0% -11.1% -11.1% -11.1% 0.0% -11.1% -11.1% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 9 Mean 11.6 24.9 25.6 28.6 25.3 20.5 14.7 95% LCL 9.71 23.3 23.7 26.6 22.8 16.2 12.9 95% UCL 13.5 26.5 27.5 30.6 27.8 24.8 16.4 Min 0 19 15 21 13 0 8 Max 17 31 33 38 34 31 23 Std Err 0.924 0.772 0.944 0.959 1.22 2.09 0.851 Std Dev 5.06 4.23 5.17 5.25 6.7 11.4 4.66 CV% 43.6% 17.0% 20.2% 18.4% 26.5% 55.8% 31.8% Diff% 0.0% -115.0% -121.0% -147.0% -118.0% -76.7% -26.4% 000-089-163-4 CETIS™v1.6.3revE Analyst: frL CETIS Summary Report Report Date: Link/Link Code: 13Feb-0913:12(p2of 2) 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep 1 1 1 1 1 1 1 1 Rep 2 1 1 1 1 1 . 1 1 Rep3 1 1 1 1 1 1 1 Rep 4 1 1 1 1 1 1 RepS 1 1 1 1 0 1 1 Rep 6 1 1 1 1 1 1 1 Rep 7 1 1 1 1 1 1 1 Rep 8 0 1 1 1 1 1 1 Rep 9 1 1 1 1 1 1 1 Rep 10 1 1 1 1 1 1 1 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 6 31 33 35 34 19 11 Rep 2 14 27 26 32 32 31 23 Rep 3 17 29 31 38 26 0 19 Rep 4 14 19 24 26 27 28 RepS 14 20 15 30 13 26 16 Rep 6 15 23 25 21 24 30 15 Rep 7 15 21 20 26 19 21 12 RepS 0 29 27 29 25 0 11 Rep 9 11 23 27 24 33 23 8 Rep 10 8 27 28 25 20 " 27 17 000-089-163-4 CETIS™ v1.6.3revE Analyst:,QA: CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:12(p1of 1) 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 03-8037-1926 Endpoint: 13Feb-0913:11 Analysis: 7d Survival Rate CETIS Version: CETISv1.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% TestStat P-Value Oecision(O.OS) Lab Control 6.25 1 12.5 1 25 0.5 50 1 100 1 Test Acceptability Attribute Test Stat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- fO.8- 0.7- !„Iff 0.5- 0.3- 0.2- 0.1- O.D Lab Control 10 0 10 0 10 0 9 1 10 0 9 0 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 10 9 9 0 6.25 12.5 25 Conc-% 50 100 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:12(p1of 2) 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 06-051 1-0252 13Feb-0913:11 Data Transform Rank Zeta Wilcoxon/BonferronI Adj Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100* Test Acceptability Attribute Control Resp PMSD ANOVA Table Source Between Error Total Endpoint: Reproduction Analysis: Nonparametric-Multiple Comparison Alt Hyp OT Test Stat 110 125 108 98.5 49.5 Monte Carlo Not Run Critical Test Stat Acceptability Ties 3 2 3 5 2 Limits 24.9 15 -NL 0.298 0.13-0.47 Sum Squares 1147.937 2406.3 3554.237 Mean Square 229.5875 45.40189 274.9893 ANOVA Assumptions Attribute Test Variances Distribution Reproduction Conc-% 0 6.25 12.5 25 50 100 Bartlett Equality of Variance Shapiro-Wilk Normality Summary Control Type Lab Control Rank Transformed Summary Conc-% Control Type 0 6.25 12.5 25 50 100 Lab Control Count Mean 10 10 10 10 10 9 24.9 25.6 28.6 25.3 20.5 14.7 Count Mean 10 10 10 10 10 9 32 34.6 41 33.4 . 27.7 9.39 OF 5 53 58 Test Stat 13.5 0.935 95% LCL 23.3 23.6 26.6 22.8 16.1 12.9 95% LCL 26.7 29 35.8 26.6 20.6 6.98 FStat 5.06 Critical 15.1 95% UCL 26.5 27.6 30.6 27.8 24.9 16.4 95% UCL 37.2 40.1 46.2 40.2 34.7 11.8 NOEL 50 P-Value 1.0000 1.0000 1.0000 1.0000 0.0005 Overlap Yes Yes P-Value 0.0007 P-Value 0.0191 0.0035 Min 19 15 21 13 0 8 Min 13.5 8.5 20 7 1.5 3 CETIS Version: Official Results: LOEL 100 Nautilus Environmental (CA) CETISvl Yes TOEL TU 70.7 2 .6.3 PMSD 29.8% Decision(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Decislon(5%) Significant Effect Decision(1%) Equal Variances Non-normal Distribution Max 31 33 38 34 31 23 Max 51 55.5 59 57 51 23.5 Std Err 0.785 0.96 0.976 1.24 2.12 0.866 Std Err 2.57 2.7 2.56 3-33 3.42 1.17 Std Dev 4.23 5.17 5.25 6.7 11.4 4.66 Std Dev 13.8 14.5 13.8 18 18.4 6.32 CV% 17.0% 20.2% 18.4% 26.5% 55.8% 31.8% CV% 43.3% 42.1% 33.6% 53.8% 66.6% 67.4% Diff% 0.0% -2.81% -14.9% -1.61% 17.7% 41.1% Diff% 0.0% -8.14% -28.3% •4.54% 13.5% 70.6% 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CET1S Analytical Report Report Date: Link/Link Code-. 13Feb-0913:12{p2of 2) 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 06-0511 -0252 Analyzed: 13 Feb-09 13:11 Endpoint: Reproduction Analysis: Nonparametric-Muitiple Comparison CETIS Version: CETISv1.6.3 Official Results: Yes Graphics 40- c 30- 1 20- 0 6.25 12.5 25 Conc-% 50 100 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 Rankits 1.0 1.5 2.0 2.5 000-089-163-4 CETIS™ v1.6.3revE Analyst:. CETIS Analytical Report Report Date: Link/Link Cods: 13Feb-09 13:12 (p 1 of 1) 18-6703-3983/0901-S034 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 19-7163-7563 Endpoint: Reproduction 1 3 Feb-09 1 3:1 1 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Resamples Linear Linear 7055475 280 Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Exp 95% CL Method Yes Test Acceptability Attribute TestStat Acceptability Limits Control Resp 24.9 15 -NL Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95% LCL 95%UCL 50 > 100 N/A N/A Reproduction Summary Conc-% 0 6.25 12.5 25 50 100 Control Type Count Lab Control 10 10 10 10 10 9 Mean 24.9 25.6 28.6 25.3 20.5 14.7 Win 19 15 21 13 0 8 Calculated Variate Max 31 33 38 34 31 23 Std Err 0.772 0.944 0.959 1.22 2.09 0.851 Std Dev 4.23 5.17 5.25 6.7 11.4 4 66 CV% 17.0% 20.2% 18.4% 26.5% 55.8% 31.8% Diff% 0.0% -2.81% -14.9% -1.61% 17.7% 41.1% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 30- § * ii 15- 10- 5- Control Type Rep 1 Lab Control 31 33 35 34 19 11 .V' ^ 0 20 40 60 Conc-% Rep 2 27 26 32 32 31 23 ~--^^\ 80 Rep 3 29 31 38 26 0 19 -. 100 120 Rep 4 19 24 26 27 28 16 Rep 5 20 15 30 13 26 15 Rep 6 23 25 21 24 30 12 Rep 7 21 20 26 19 21 11 Rep 8 Rep 9 29 23 27 27 29 24 25 33 0 23 8 17 Rep 10 27 28 25 20 27 000-089-163-4 CETIS™ v1.6.3rev£Analyst:. CETiS Analytical Report Report Date: Link/Link Code: 13Feb-0913:20(p1of 1} 18-6703-3983/0901-S034. , Ceriodaphnia 7-d Survival and Reproduction Test ' Analysis No: 07-5121-3402 Endpoint: Reproduction Analyzed: 13 Feb-09 13:19 Analysis: Parametric-Two Sample Data Transform Untransformed Zeta Alt Hyp Monte Carlo NOEL C > T Not Run <0 Equal Variance t Two-Sample Test Control vs Control TestStat Critical MSD P-Value Lab Control Conductivity Control 6.38 1.73 3.62 0.0000 Test Acceptability Attribute Control Resp Control Resp PMSD TestStat Acceptability Limits Overlap 24.9 15 -NL Yes 11.6 15 -NL Yes 0.145 0.13-0.47 Yes ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Between 884.45 Error 391.3 884.45 1 40.7 0.0000 21.73889 18 Nautilus Environmental (CA) CETIS Version: CETISvl.6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 14.5% Decision{5%) Significant Effect Decision Passes acceptability criteria Fails acceptability criteria Passes acceptability criteria Decrsion(5%) Significant Effect Total 1275.75 906.1889 19 ANOVA Assumptions Attribute Test TestStat Critical P-Value Variances Variance Ratio F 1.43 6.54 0.6010 Distribution Shapiro-Wilk Normality 0.919 0.0964 Reproduction Summary Conc-% Control Type » Count Mean 95% LCL 95% UCL Win 0 Lab Control 10 24.9 23.3 26.5 19 0 Conductivity Co 10 11.6 9.68 13.5 0 Graphics 40- c 30-_o w 20- .L -*— 10-1 * If o -s RejecfNuli 10~ Decision(1%) Equal Variances Normal Distribution Max StdErr Std Dev CV% Diff% 31 0.785 4.23 17.0% 0.0% 17 0.94 5.06 43.6% 53.4% ^0 0 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Sr^&lnc--M, L. C- Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst:.QA: Freshwater Chronic Bioassay Client: Dudek & Associates Water Quality Measurements Test Species: C. dubia Sample ID: Upstream Test No: Start Date/Time: 1/16/2009 I32.O End Date/Time: 1/23/2009 Concentration Day pH Cond. (pmhos/cm) TempC) Lab Control g.i< 71 \cl 3&i PH DO (mg/L) Temp CC)i. f .0 Concentration Da Conductivity Control PH DO (mg/L) Cond. ((imhos/cm) Temp (°C) 1 a H40O 16.6 S-2f 8- ft PH DO (mg/L) Temp (°C) lff S5.5 25.4 ^-03S.I £5.0 %w Concentration Day 6.25% PH DO (mg/L) Cond. (ijmhos/cm) Temp (°C) 7-S JLE PH DO (mg/L) Temp (°C) 5*13 243 s-ys--7 .5 507 2V. £ zo 2S-H 1.1 Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: MO MD A- HP St A S*- s «•'* V Sample Log-in Numbers: A: QC Check: B: Animal Age at Initiation: C: Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client: Dudek & Associates Sample ID: Upstream Test No: Test Species: C. dubia Start Date/Time: 1/16/2009 i3Z-(7 End Date/Time: 1/23/2009 < > P V Concentration 25% Day ^K£.,.'.;~%.£«^*.^.>£i^\* pH :M2. -r,y <z.c&T^5 DO (man.) Concentration 50% Da pH DO (mq/U Cond. (pmhos/cm) Temp ('Cl Tf5 S-i fc-3 7-*U 7.83 1.6 7-7?«. a 24. f Concentration 100% Da JL.A3njfial PH DO(mg/L)-1 TT5 7.70 1--? Cond. ((jmhoa/cm)MMM6 Temp ('C ZST.7 PH 00 (mgO.) Temp CO S S-l Analysts: Initial: Final: Sample Used (A, B, C): Comments: MO MO rV KD SL B: Animal Source/Date Received: jUtff.fflQ.J /ft Sample Log-in Numbers: A: C~f\ — Of/sP QC Check: '{J— ^li^jO^ NtuUlui Environment*! $550 Warehouse Drive, Suite 1SO. S*n Diego, CA 92121. Animal Age at Initiation:. C: Kr s Final Review: Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates - Upstream Test Number: CROH Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Datemme: 1/16/2009 End Date/Time: 1/23/2009 Cone. LC Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup I I/ ^7 1°13 15 *nM •u *lo Jib•asr Daily Reproduction/ Survival :i:!:;:jl!:|:|:j O 0ooooo 0o c> Mt> ^ j-iljij&ijiii O O O(? Oo o (7 WP^ •::r':°3*i'!:i 0 o 0o 0 0 65 fr. ( & 0o E5 lilijIlS&H:£ t ^ ^* 4 5"3 O 5" -7t| SL Siij'Sljii!! Coc» 76 O lO& C O St- !i!l?!!3IH!!!Sl! 5 K 1/0 6 •7 & 1C 4 l,rt H :j::j!:?|:|i|i| 1*1 \ ^ HO 0, , >L o K(^ i 3- //rf !|!|!j:8.:j:j:i|Total i> 1 3~1 31. °l 1 1 ;>O 3«?? 9 I •g.'H 31 'J? -*11"" 1 QC £ P* Cone. CC Rep 1 2 i_ 34 5 6 7 8 9 10 Cone. 6.25% Cone. 12.5% Rep 1 2 3 4 5 6 7 8 9 10 Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival Ihiili!6oooo fl c\ o O O ji::[:j?!ii:|: U nO0 O r) D O/iJU Ou en!:!*!:!:!e o 6 0 6 0 ft —0 /> ii=:iif;;i;;: 0 U ^i( A 0 I_ ^J0 ;;!:;:5:;;;;i u o 0 O O 5 <3 ~- ' C Z mm*tf s< <»•^.— H•5 i:::;;i7;i:;:::o 5<{ S ? "7 l( "• S O ::i;;!®:!:!j) - Total @> \ H . V^o/<^ I \ £> Daily Reproduction/ Survival •Jjj:::j; O oo t) 0 onf\ o,-t ::;j;:;2;li;;i: 0oouc) i) O cJ0 f !:;i!:;?::|:!i P) 0 Oo 0 0 3 oo o |::::::4:::i|: 5^s* <§• ^S" (^ G £> ty |:!:;:|5j:!:!: O 8o\n oto-Jo 10a !:|ii:S:|:!:i-» o 0*^» \*b fag cr (0 1 A, i -31 H:!:!*-:!:!;! l'c 1*5 ^ S^, 1 0Vsusp, 1 2^^[ I C» jii^jftj:!:!:Total 33J •J.(0 •^ 3° V5> 5LS•3.0 3"-^ 31as* Daily Reproduction/ Survival .. lijiliii Ooo £) rt o /) oa V •:;:!:•«•:•::: 0z>c;no 0 0t7 0 0 |:!:|i»j:!:|: r> O £> 0 (5 O 3, 0 O c? iliniiSijij'i.r ^"^ i<^- 3o 5T U "5 IlilJilSn!:;: O U O \-^ \\o \t o ^a :;:-::JP:::i::.<i| Om« o-? {a f fi•7 ;i:i;ii7i;i=;i; 2_i l-y l"?(5 fZ, (i O 15 V5 :ijH:WJ:jii;Total "2>?p '2>$- ""y'a) ^3(^7 "?»oy<\y,(^>&.'-] PH QC f1M QC W ^8 QC JoLl»is ime Fed/Test Solution Renewed (day): ,JHOO_(1). ' 1050 (61 1345 (7) lotes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are included in total) omments: 1C Check:Final Review: Jautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates - Upstream Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 1/16/2009 \3 Z0 End Date/Time: 1/23/2009 it. Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup 1 >/ 3 -7 \ 0 13 >S Ifa 17 H Z.O 2-t rrt Efe Daily Reproduction/ Survival\mm* 0oo 0 o oa q 0 0 d/lp ijillj!*!!!!!! O O o O O 0 o 0 to V w~ m&m -0 o o 0 0 0 2 0 o O €S lHji&i!!*m i << <<( < c > >6»r></><£>3'H?oi 6a bet 0 3r O to <}/M <? "7 <3 h 0 3k iiiiiiiSiiiiii U 0 ^t>^t(_ ^1fr 0 7 V(V i!i!!i*HIHl 1*7 K V^L. O *. id 0iv)c-tu Mb mm* — Total *>M ^b^>PU ^^A|^l^ 3^IS ^e ^3 ?o QC 1) /** Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival 1 Oo O G 0 a f\ a 0d 2 0 0 O O U U 0 0 u u a ; n f\ O 0 0 0 2. 0 C?0 4ar o T \5"so 0s«? M,Jf6 ! o 0 to 5 Q "7 O <? 0 fr ^Oo 0 1I'k Ike? o £ f ' ^OPe> \^\<\5o O V( t T 9 Total A °l "2>\ 0 *•'& 3-^ff -^,0 31 10^^>an QC IT^ Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival &:;:!: Oa 0 ^q6 0o 0 0 ih:!i!2;i:36ot> o O V 0 Ut> '0 O 0 0 0 0 60 6 <?. 6 !:;£$!!!!!; O 5* •^ 3 O tf O G O c> iiiii!£i|i£i O 6 0 0 •7 O 5" o H O ililliHlili!!! *tb $ iA$ 0 Co 77 l/ 4 iiiiiii^iiiii; v l\ V2) ^> ^fi o '1611 ii!i!ii^Ni;ii — Total ll 3: 1 c u-> 9 L^>'Z-j €>n QC Time Fed/Test Solution Renewed (day):L (050 i j^»g (7) Notes: d = dead; M = male; HP = lost in progress; B = 4th brood (only the first 3 broods are Included In total) Comments: QC Check:Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Site: Well #3 CETIS Summary Report Report Date: Link/Link Code: 13Feb-0914:45(p1of 2) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Test Run No: 05-5784-1439 Test Type: Reproduction-Survival (7d) Start Date: 16 Jan-09 14:00 Protocol: EPA/821/R-02-013 (2002) Ending Date: 23 Jan-09 10:40 Species: Ceriodaphnia dubia Duration: 6d 21h Source: In-House Culture Sample No: 16-2554-5040 Code: 09-0031 Sample Date: 15 Jan-09 10:30 Material: Groundwater Receive Date: 16 Jan-09 10:10 Source: Dudek & Associates Sample Age: 28h(11.2'C) Station: Well 3 Comparison Summary Analysis No Endpoint 1 3-6534-61 65 7d Survival Rate 2 1 -3835-9055 Reproduction Point Estimate Summary Analysis No Endpoint 01-8027-3804 7d Survival Rate 17-7781-5640 Reproduction Test Acceptability Analysis No Endpoint 01-8027-3804 7d Survival Rate 1 3-6534-61 65 7d Survival Rate 17-7781-5640 Reproduction 21-3835-9055 Reproduction 21-3835-9055 Reproduction 7d Survival Rate Summary Conc-% Control Type Count 0 Conductivity C 10 0 Lab Control 10 6.25 10 12.5 10 25 10 50 10 100 9 Reproduction Summary Conc-% Control Type Count 0 Conductivity C 10 0 Lab Control 10 6.25 10 12.5 10 25 10 50 10 100 9 NOEL 50 12.5 Effect-% 50 50 Attribute Control Resp Control Resp Control Resp Control Resp PMSD Mean 95% LCL 0.6 0.407 1 1 1 1 1 1 1 1 0.9 0.782 0.111 0 Mean 95% LCL 3.9 2.22 26.1 25.1 22.8 21.3 20.2 16.7 19.5 17.6 9.5 7.02 0 0 LOEL 100 25 Conc-% 71.1 41.1 TestStat 1 1 26.1 26.1 0.233 95% UGL 0.793 1 1 1 1 1 0.236 95% UCL 5.58 27.1 24.3 23.7 21.4 12 0 TOEL 70.7 17.7 95% LCL 62.7 34.6 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client: Dudek & Associates Project: PMSD N/A 23.3% 95% UCL 80.5 49 Acceptabilty Limits 0.8 - NL 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Min 0 1 1 1 1 0 0 Min 0 21 17 0 10 1 0 Max 1 1 1 1 1 1 1 Max 14 31 30 31 25 19 0 Method Fisher Exact/Bonferroni-Holm Test Wilcoxon/Bonferroni Adj Test Method Trimmed Spearman-Karber Linear Interpolation (ICPIN) Overlap Decision Yes Passes acceptability criteria Yes Passes acceptability criteria Yes Passes acceptability criteria Yes Passes acceptability criteria Yes Passes acceptability criteria StdErr Std Dev CV% Diff% 0.0943 0.516 86.1% 0.0% 0 0 0.0% -66.7% 0 0 0.0% -66.7% 0 0 0.0% -66.7% 0 0 0.0% -66.7% 0.0577 0.316 35.1% -50.0% 0.0609 0.333 300.0% 81.5% Std Err Std Dev CV% Diff% 0.823 4.51 116.0% 0.0% 0.49 2.69 10.3% -569.0% 0.754 4.13 18.1% -485.0% 1.7 9.32 46.1% -418.0% 0.908 4.97 25.5% -400.0% 1.21 6.64 69.9% -144.0% 0 0 100.0% 000-089-163-3 CETIS™ v1.6.3revE Analyst: CETIS Summary Report Report Date: Link/Link Code: 13Feb-0914:45(p2of 2) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Reproduction Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Detail Control Type Conductivity Co Lab Control Rep1 0 1 1 1 1 1 0 Rep1 0 26 28 27 23 3 0 Rep 2 1 1 1 1 1 1 0 Rep 2 14 27 30 26 13 11 0 Rep 3 0 1 1 1 1 1 0 Rep 3 0 28 22 9 25 4 0 Rep 4 1 1 1 1 1 1 0 Rep 4 5 25 23 31 22 17 0 Rep 5 0 1 1 1 1 1 0 Reps 0 21 17 17 16 11 0 Rep 6 0 1 1 1 1 1 Rep 6 0 31 20 21 22 16 Rep 7 1 1 1 1 1 0 1 Rep 7 7 28 20 22 21 1 0 Rep 8 1 1 1 1 1 1 0 Rep 8 6 26 19 25 24 19 0 Rep 9 1 1 1 1 1 1 0 Rep 9 2 25 23 0 19 11 0 Rep 10 1 1 1 1 1 1 0 Rep 10 5 24 26 24 10 2 0 000-089-163-3 CETlS™v1.6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:30(ptof 1) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 13-6534-6165 Endpoint: 7d Survival Rate Analyzed: 13 Feb-09 13:29 Analysis: STP 2x2 Contingency Tables Data Transform Untransformed Zeta Alt Hyp C>T Fisher Exact/Bonferroni-Holm Test Control vs Conc-% Test Stat Lab Control 6.25 12.5 25 50 100 Test Acceptability Attribute Control Resp Data Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Graphics l.O-i • • 0.9- v 0:8- * 0.7- 1 o.e $ 0.5- S o., 0.3- 0.2- *1 0 6.25 1 1 1 0.5 0.000119 Monte Carlo NOEL Not Run 50 P- Value Decision(O.OS) 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 0.000595 Significant Effect Test Stat Acceptability Limits Overlap 1 0.8 No-Resp Resp 10 0 10 0 10 0 10 0 9 1 1 8 • • • 12.5 25 SO Conc-% - NL Yes Total 10 10 10 10 10 9 • 100 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes LOEL TOEL TU PMSD 100 70.7 2 N/A Decision Passes acceptability criteria 000-089-163-4 CETIS™ v1.6.3revE Analyst:, CETfS Analytical Report Report Date: Link/Link Cods: 13 Feb-09 13:30 (p 1 of 1) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 01-8027-3804 13 Feb-09 13:29 Endpoint: Analysis: Spearman-Ka'rber Estimates Threshold Option Threshold Trim Control Threshold 0 Test Acceptability Attribute Control Resp 7d Survival Rate Summary 11.110/ Test Stat 1 7d Survival Rate Trimmed Spearman-Karber Mu i 1.85 Acceptability 0.8 - NL Sigma 0.027 Limits Overlap Yes CETIS Version: Official Results EC/LC50 71.1 Decision 95% UCL 62.7 Nautilus Environmental (CA) CETlSvl.6.3 Yes 95% UCL 80.5 Passes acceptability criteria Calculated VarIate(A/B) Conc-% Control Type Count Mean 0 Lab Control 10 6.25 10 12.5 ' 10 25 10 50 10 100 9 7d Survival Rate Detail Conc-% Control Type Rep 0 Lab Control 1 6.25 1 12.5 1 25 1 50 1 100 0 Graphics 1.0 ( 0.9- 0.8- g 0.7- * 0.6-s ; '§ 0.5- 5 a"~ 0.3- 0.2- 0.1- 0.0( ^A 1 1 1 1 0.9 0.111 1 Rep 2 1 1 1 1 1 0 \ ) 20 40 60 80 Conc-% Win 1 1 1 1 0 0 Rep 3 1 1 1 1 1 0 \ 100 120 Max 1 1 1 1 1 1 Rep 4 1 1 1 1 1 0 Std Err 0 0 0 0 0.0577 0.0609 Rep 5 1 1 1 1 1 0 Std Dev 0 0 0 0 0.316 0.333 Rep 6 1 1 1 1 1 1 CV% 0.0% 0.0% 0.0% 0.0% 35.1% 300.0% Rep 7 1 1 1 1 0 0 Diff% A B 0.0% 10 10 0.0% 10 10 0.0% 10 10 0.0% 10 10 10.0% 9 10 88.9% 1 9 Rep 8 Rep 9 Rep 10 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0 000-089-163-4 CETIS™ v1.6.3revE Analyst;. CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:30(p2of 3) 07-0484-2794/0901-S033 Cariodaphnla 7-d Survival and Reproduction Test Analysis No: Analyzed: 21-3836-9055 13Feb-0913:29 Data Transform Rank Zeta Wilcoxon/Bonferroni Adj Test Control vs Conc-% Lab Control 6.25 12.5 25* 50' 100* Test Acceptability Attribute Control Resp PMSD ANOVA Table Source Between Error Total Endpoint: Reproduction Analysis: Nonparametric-Multiple Comparison Alt Hyp C>T Test Stat 80 82 63 55 45 Monte Carlo Not Run Critical Test Stat Acceptability Ties 2 6 3 0 0 Limits 26.1 15 -NL 0.233 0.13-0.47 Sum Squares 4484.697 1619.1 6103.797 Mean Square 896.9393 30.54906 927.4884 ANOVA Assumptions Attribute Test Variances Distribution Reproduction Conc-% 0 6.25 12.5 25 50 100 Mod Levene Equality of Varianc Shapiro-Wilk Normality Summary Control Type Lab Control Rank Transformed Summary Conc-% Control Type 0 6.25 12.5 25 50 100 Lab Control Count 10 10 10 10 10 9 Count 10 10 10 10 10 9 Mean 26.1 22.8 20.2 19.5 9.5 0 Mean 48.5 38.5 36 31.4 17.7 5.5 DF 5 53 58 Test Stat 3.38 0.941 95% LCL 25.1 21.2 16.7 17.6 6.98 0 95% LCL 45.6 34 29.5 27.6 15.6 5.5 FStat 29.4 Critical 3.38 95% UCL 27.1 24.4 23.7 21.4 12 0 95% UCL 51.4 43 42.5 35.2 19.7 5.5 NOEL 12.5 P-Value 0.1260 0.1780 0.0015 0.0000 0.0000 Overlap Yes Yes P-Value 0.0000 P-Value 0.0100 0.0065 Min 21 17 0 10 1 0 Min 32 24 5.5 16 11 5.5 CETIS Version: Official Results LOEL TOEL 25 17.7 Decision(5%) Non-Significant Effect Non-Significant Effect Significant Effect Significant Effect Significant Effect Decision Nautilus Environmental (CA) CETISv1.6.3 Yes TU PMSD 8 23.3% Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Decision(1 %) Equal Variances Non-normal Distribution Max Std Err 31 0.499 30 0.767 31 1.73 25 0.923 19 1.23 0 0 Max Std Err 58.5 1.43 57 2.19 58.5 3.17 45.5 1.84 27 0.987 5.5 0 Std Dev CV% 2.69 10.3% 4.13 18.1% 9.32 46.1% 4.97 25.5% 6.64 69.9% 0 Std Dev CV% 7.71 15.9% 11.8 30.6% 17.1 47.4% 9.93 31.6% 5.31 30.1% 0 0.0% Diff% 0.0% 12.6% 22.6% 25.3% 63.6% 100.0% Diff% 0.0% 20.6% 25.8% 35.3% 63.6% 88.7% 000-089-163-4 CETIS™ v1.6.3revE Analyst:.. CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:30(p3of 3) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 21-3835-9055 Endpoint: Reproduction Analyzed: 13 Feb-09 13:29 Analysis: Nonparametric-Multiple Comparison CETIS Version: CETISvl.6.3 Offtcial Results: Yes Graphics 10-1 30- 10- 6.25 12.S 25 Conc-% -RejedWoll-i 50 100 -40 -2.5 -2.0 -1.5 -1.0 -0.5 0.0 O.S 1.0 1.5 2.0 2.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: 1 3 Feb-09 1 3:31 (p 1 of 1 ) Link/Link Code: 07-0484-2794/090 1-S033 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No Analyzed: 17-7781-5640 Endpolnt: Reproduction 13 Feb-09 13:29 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Linear Resamples Linear 2895625 280 Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Exp 95% CL Method Yes Test Acceptability Attribute TestStat Acceptability Limits Control Resp 26.15-NL Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 41.1 34.6 49 Reproduction Summary Conc-% Control Type Count 0 6.25 12.5 25 50 100 Lab Control 1 0 10 10 10 10 9 Calculated Variate Mean 26.1 22.8 20.2 19.5 9 0 .5 Min 21 17 0 10 1 0 Max 31 30 31 25 19 0 Std Err 0.49 0.754 1.7 0.908 1.21 0 StdOev 2.69 4.13 9.32 4.97 6.64 0 CV% 10.3% 18.1% 46.1% 25.5% 69.9% Diff% 0.0% 12.6% 22.6% 25.3% 63.6% 100.0% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 30" 25- o•f 10- 0c Control Type Rep 1 Lab Control 26 28 27 23 3 0 I V V 20 40 60 Conc-% Rep 2 27 30 26 13 11 0 \ \ so Rep3 28 22 9 25 4 0 -v, 100 120 Rep 4 25 23 31 22 17 0 Rep 5 21 17 17 16 11 0 Rep 6 31 20 21 22 16 0 Rep 7 28 20 22 21 1 0 Rep 8 Rep 9 Rep 10 26 25 24 19 23 26 25 0 24 24 19 10 19 11 2 0 0 000-089-163-4 CETIS Tttv1.6.3revE Analyst: r L> CETIS Analytical Report flab CodstA vV. Report Date: Link/Link Code: 13Feb-0913:30(p 1 of 3) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: 13-3142-7496 Endpoint: Reproduction Analyzed: 13 Feb-09 13:29 Analysis: Parametric-Two Sample Data Transform Untransformed Zeta 1 / Nautilus Environmental (CA) CETIS Version: CETISvl .6.3 Official Results: Yes Alt Hyp Monte Carlo NOEL LOEL TOEL TU PMSD C>T Not Run <0 0 N/A 11.0% Equal Variance t Two-Sample Test Control vs Control Lab Control Test Acceptability Attribute Control Resp Control Resp PMSD ANOVA Table Source Between Error Total TestStat Critical MSD P-Value Decision(5%) Conductivity Control 13.4 1.73 2.88 0.0000 Significant Effect TestStat Acceptability Limits Overlap Decision 26.1 15 -NL Yes Passes acceptability criteria 3.9 15 -NL Yes Fails acceptability criteria 0.11 0.13-0.47 • Yes Fails acceptability criteria Sum Squares Mean Square OF F Stat P-Value Decision(5%) 2464.2 2464.2 1 179 0.0000 Significant Effect 247.8 13.76667 18 2712 2477.967 19 ANOVA Assumptions Attribute Test Variances Distribution TestStat Critical P-Value Decision{1%) Variance Ratio F 2.82 6.54 0 Shapiro-Wilk Normality 0.923 0 Reproduction Summary Conc-% Control Type Count 0 Lab Control 10 0 Conductivity Co 10 Graphics 10- | ^ 1 20- 10- 0 ' 1390 Equal Variances 1140 Normal Distribution Mean 95% LCL 95% UCL Win Max Std Err Std Dev CV% Diff% 26.1 25.1 27.1 21 31 0.499 2.69 10.3% 0.0% 3.9 2.19 5.61 0 14 0.837 4.51 116.0% 85.1% 1 '— •*— 1 Reject-Nun— i 0 0 CO- Conc-% LC, IS-, il / = ^***^»*-» • j»--» • -5- • ^^ -10 — T "1 "• ~r — r — • — 1 T 1 •2.0 -l.S -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst:.QA: CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:31(p1 of 1) 07-0484-2794/0901-S033 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-6233-1514 Analyzed: 13 Feb-09 13:29 Endpoint: 7d Survival Rate Analysis: Single 2x2 Contingency Table CETIS Version: Official Results: CETISvl.6.3 Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEU TOEL TU PMSD Untransformed C>T Not Run <0 N/A N/A Fisher Exact Test Control vs Control TestStat P-Value Decision(O.OS) Lab Control Conductivity Control 0.0433 0.0433 Significant Effect Test Acceptability Attribute TestStat Acceptability Limits Overlap Decision Control Resp Control Resp 1 0.6 0.8 - NL 0.8 - NL Yes Passes acceptability criteria Yes Fails acceptability criteria Data Summary Conc-% Control Type No-Resp Resp Total Conductivity Co 6 4 10 Lab Control 10 0 10 Graphics 1.0- 0.9- 0.8- 0.7- 0.6- 0.5- 0.4- 0.3J 0.2- 0.1- 0.0 Conc-% 000-089-163-4 CETIS™ v1.6.3revE Analyst:_ Freshwater Chronic Bioassay ilient/Sample ID: Dudek & Associates - Well 3 Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Cone. LC Rep 1 2 3 4 5 6 7 8 9id Analyst Verified by: Board/ Cup 7. ' \f <(f2i Z-'i2.H 15 Z£ t? •2.1 50 31 32- £5 .Tf, Daily Reproduction/ Survival :j:i:!i&j£! A *(7o(5 0 0a. G O sI6 " :;::!:i?i:i:jj e? O 0& 0 0 0 0 0 9Hr & Q0 O f\o pc) 0 • &» -CA >5 6 6 <ff h•5" >H iijijiSiliiii Oo P£> (^e>ft oo r\nt wmm\-*fif*v Of -& H ^1 *> -1 I*3 »^<_ !i|ij:l^:iiiiiiy \ \ \ ^In ^i 5 i3v iff 10 /t/hL ;jj::|l^:|:::j Total S(e f-N — t ^^ * ^Q JJ-^ ^•1•3,1 5t/3 •2^ ^M QC \l &/*~~ Cone. CC Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival JHSljM:!;; 46> <? O 0O/D1 cr, Qa vf/L6 n/A> °tojoU — O Ooo Hi;!;a;!:hl— (0 / m--- O *- __op 0 0 i;|i;;S!*l; ^, 1 •—o • — — 6 0 O O IlililSllH!!^_. .^— r>_*— - /3_ ^oO IllOiSiii!!!! —•«=, — «< —•— ^Md ^ j|i:!j;?|j|j:i:_ ? . rt- - Od> cO O j!!i!i®!i:ii: '- Cone. 6.25% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival •=:!;!!|!!!;!j! O a Q a C}o G a O & mmm 0oo Q C?-o 0 Z) O U !!!l!!!?!i;i!i 0 0 h ft 0 0 Q0 0 0 l;!:;:!4!=;i!s 5 M r-s- s ^ •5- <( ^fq *•z liliiliSillii!; O Oo ID ~7o« O O O mmm- <*\*> IO o O•ac * c • c. 1 ;;;:;;i7;*!;; ^I? 7a % %7 (£> H»v |ii:;:!ft!:i;|i Total C^a \«-fo I <i^>^>/do/ <i_ ( (^ 3 ^ Total "3-tS ^.0 S'it 23n 3.0 ^JO 'I ^2i(j> _*- ^ Cone. 12.50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival :i:]:i!#IH d G Oo o 0a qa /7 =;=;*?;*;;c>o U ^cJ •o0o "0 (_/ iiiiHlailjij! ^n O h f) 6 rtn 0 0 iyiiiiSiliiii 5- ^" - Q £j5f •5 .? 3<9 .? iiliHlMil;•o c)on G» Oy O O G O iliiilueliliill -7. <3| — ' I?o«crn?!M (D1 i;i;i;7iii;ii; if^Nr^x SU-r^•Ns "7 ^l.fi fjrV\x i;li!:i8i;i:!;:Total ^"?•r>tiq^»n^i^^S^><J 3H QC fljit^ QC 0(&L f\V<- QC (oex. me Fed/Test Solution Renewed (day):V\<\Q otes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included In total) >mments: C Check:Final Review: autilus Environmental 5550 Morehouso Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates - Well 3 Test Number: Oaphnid Survival and Reproduction Datasheet Test Species: C.dubia D. pulex D. magna Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup 2 ' li 2,3 if-M Z.S U z.7 11 So 11 32. ES 3C, Daily Reproduction/ Survival iHiiiijij!!!:;! & o 0 a 0 (3 Q 0 o o JC ;i;=|:;&£;i & O O 0o O V O O o Vf~ ;£|ii3;;i:=;;d 00fi<o 90 0ft0 Vivr !:i:;:J4i|:|i!lH H ^ ^3 s 3 I o SL ^i^iiiil; O Oooou 0 ©fi Hw |i||!|iSl:ili: (an 1 5? Mto 7 7 6'^ DP ii;:!i;5hiiii!i \>} } 1^ 1^10 fl t?) l^f i7) d) /v^ !ji!&8ijiil!Total ^3 "» "^> 3-3 2=^ !U 3a 3 1 3-M 1 °^ 10 QC M t%_ Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival .$HI3 G 0 0 0 Q O Q 0 O <3 iilHiaiil:!!. C? O O & O 0 t> O Oo IliHiaiiiHiio 0o 0 Qo 6 I? 06 !!!ii;l*i[l!l!io oo M ^3 d/lo 3 o !!lHi*!!!j!!3/? 4ao n— ,«?oo iHiii^iiii;:^> 0 0 2( 3 f — ^3 & mmxooa q *fIi3 —11 fon iiiiiiiilsiiiiH '— Total *5> 1 I Mn1 1 }(£> *l$t°i1 1 3- QC t5l«- Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival Hi!*!; O (?<y (7 a c, 0 0, d/n'n. m&mQIO^o OM-o O 0 Q 0/ks - C> mmm• — _ oj<*^ . b 0 L-XP 0—-~ 0 nii!!NN!i!!i! — -< — 0a/6•t 0_— ^.^^o !!i!!!*ii!. — — —o/c| —- o — — — !i!!j!3ij!!ij! — —_ — • — ^ C> — __. — iii!=!lJ1i{!!i! _— — ~~__ _— ~o— — — !i!i!i!*!i!!!J ,^ — -i— —^ V— ~. — Total 0/d o/(i c/ci 6/<4ws L-tP O oK0/4 ^/cL- QC O VI Time Fed/Test Solution Renewed (day): .(D_ Notes: d = dead; M = male; LIP = lost in progress; B » 4th brood (only the first 3 broods are included in total) Comments: QC Check:Final Review: Nautilus Environmental 5550 More/rouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client: Dudek & Associates Water Quality Measurements Test Species: C. dubia Sample ID: Well 3 Test No: Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Concentration Day PH OO(mq/L) Cond. (pmhos/cm) TempCO PH DO (man.) Temp (*C) Lab Control R-IS •2.5-3 1-fc Si'?/" l"7.TLST 7-no? £."23 -&**: oO.| S^*J Concentration Da pH DO (ma/L) Cond. (pmhos/cm) Temp CO Conductivity Control ^TP ; ,- pas BXJfl PH DO(mg/U Temp (-C)-7.V.6 «-07 8.¥ 7.S-4 -7.7? Concentration Day 6.25% PH DO (mg/L) Cond. ((jmhos/cm) Tem °C) PH DO (mo/U Temp (°C) 7H5jj. s « 7,^7 .. - J9p. 71 J I 6(0 -7.96 •8.0 S-% 25-^ Concentration Dajf 12.5%» 1. » PH DO (mg/L) Cond. (pmhos/ctn) Temp ("C) l-7to 1 «5 7-2 975as-3 l-S PH DO (mg/L) Temp CC) 7.7 [a 7.7 i -7. -9S HA . o . 6 Analysts: Initial: Final: Sample Used (A, B, C): MO , A MO J6A for rT /^ o£ Ht? ^ ^>L Df A DP D^A su AJ(A + ^- -L: K> f \ Comments: Animal Source/Date Received: Sample Log-in Numbers: A: QC Check: B: Animal Age at initiation: C: . Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality Measurements Client Dudek & Associates Sample ID: Well 3 Test No: Test Species: C. dubia Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Concentration Day 100% 0 | 1j 2 I 3 [ 4 j 8 I 6 PH''" 1703 | 7-33 | 7-0*17. DO (mg/L) Cond, (pmhos/cm) 250 71 PH DO (mg/L) Temp CC) &]L 0^- 3.5- 3,3 25 '8.1?, 35- O 8./-T jj£fe Analysts: Initial: Final: Sample Used (A, B, C): Comments: _ Animal Source/Date Received: _ MO MO 6 MO Sc HO Sample Log-in Numbers: A: 0*%- 3l Animal Age at Initiation: C: hf 3 QC Check: Nautilus Environmental 5550 Moreftous* Drive, Suite f 50. Sm D/ego, CA 9212}. Final Review: / Site: Well#1 CETIS Summary Report Report Data: Link/Link Code: 13 Feb-09 13:40 (p 1 of 2) 14-7882-2846/0901-S032 Certodaphnia 7-d Survival and Reproduction Test Test Run No: 06-3019-1555 Test Type: Reproduction-Survival (7d) Start Date: 16 Jan-09 14:00 Protocol: EPA/821/R-02-013(2002) Ending Date: 23 Jan-09 10:05 Species: Ceriodaphnia dubia Duration: 6d 20h Source: In-House Culture Sample No: 00-6709-5228 Code: 09-0032 Sample Date: 15 Jan-09 10:45 Material: Groundwater Receive Date: 16 Jan-09 10:10 Source: Dudek & Associates Sample Age: 27h(11.2*C) Station: Well 1 Comparison Summary Analysis No Endpoint 1 7-0360-2041 7d Survival Rate 17-7765-6394 Reproduction Point Estimate Summary Analysis No Endpoint 21-3244-7817 Reproduction Test Acceptability Analysis No Endpoint 17-0360-2041 7d Survival Rate 17-7765-6394 Reproduction 21-3244-7817 Reproduction 1 7-7765-6394 Reproduction NOEL LOEL 100 >100 50 100 Effect-% 50 Attribute Control Resp Control Resp Control Resp PMSD Conc-% 72.2 Test Stat 1 26 26 0.218 TOEL N/A 70.7 Nautilus Environmental (CA) Analyst: Diluent: Diluted Mineral Water (8:2) Brine: Not Applicable Age: <24h Client: Dudek & Associates Project: PMSD N/A 21.8% Method Fisher ExacfBonferroni-Holm Test Steel Many-One Rank Test 95% LCL 95% UCL Method 67.4 74.9 Linear Interpolation (ICPIN) Acceptabilty Limits Overlap 0.8 - NL 15 -NL 15 -NL 0.13-0.47 Yes Yes Yes Yes Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Survival Rate Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 10 Mean 1 1 1 0.9 1 1 0.9 95% LCL 1 1 1 0.782 1 1 0.782 95% UCL 1 1 1 1 1 1 1 Min 1 1 1 0 1 1 0 Max 1 1 1 1 1 1 1 Std Err 0 0 0 0.0577 0 0 0.0577 Std Dev 0 0 0 0.316 0 0 0.316 CV% 0.0% 0.0% 0.0% 35.1% 0.0% 0.0% 35.1% Diff% 0.0% 0.0% 0.0% 10.0% 0.0% 0.0% 10.0% Reproduction Summary Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity C Lab Control Count 10 10 10 10 10 10 10 Mean 17.1 26 24.8 24.7 26.4 23.4 0 95% LCL 14.3 24.6 23.4 20.7 25 21.5 0 95% UCL 19.9 27.4 26.2 28.7 27.8 25.3 0 Min 4 17 16 0 20 15 0 Max 24 30 30 36 32 31 0 Std Err 1.36 0.699 0.693 1.97 0.69 0.916 0 Std Dev 7.48 3.83 3.79 10.8 3.78 5.02 0 CV% 43.7% 14.7% 15.3% 43.6% 14.3% 21.4% Diff% 0.0% -52.0% -45.0% -44.4% -54.4% -36.8% 100.0% 000-089-163-3 CETIS™ v1.6.3revE Analyst:. CETIS Summary Report Report Date: Link/Link Code: 13Feb-0913:40(p2of 2) 14-7882-2646/0901-S032 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) 7d Survival Rate Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 1 1 1 1 1 1 1 Rep 2 1 1 1 1 1 1 1 Rep 3 1 1 1 1 1 1 1 Rep 4 1 1 1 1 1 1 1 RepS 1 1 1 1 1 1 1 Rep 6 1 1 1 1 1 1 0 Rep 7 1 1 1 1 1 1 1 Rep 8 1 1 1 0 1 1 1 Rep 9 1 1 1 1 1 1 1 Rep 10 1 1 1 1 1 1 1 Reproduction Detail Conc-% 0 0 6.25 12.5 25 50 100 Control Type Conductivity Co Lab Control Rep1 4 17 16 14 20 20 0 Rep 2 4 24 24 28 26 24 0 Rep 3 23 30 26 25 27 31 0 Rep 4 16 30 30 34 27 21 0 RepS 16 26 29 33 31 29 0 Rep 6 21 26 23 29 21 15 0 Rep 7 20 25 24 26 27 27 0 Rep 8 24 28 25 0 25 27 0 Rep 9 24 29 26 36 32 20 0 Rep 10 19 25 25 22 28 20 0 000-089-163-3 CETIS™ v1.6.3revE Analyst:, CETIS Analytical Report Report Data: Link/Link Code: 13Feb-0913:39(p1of 1) 14-7882-2646/0901-S032 Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: Analyzed: 17-0360-2041 Endpoint: 13Feb-0913:38 Analysis: 7d Survival Rate CETIS Version: CETISv1.6.3 STP 2x2 Contingency Tables Official Results: Yes Data Transform Zeta Alt Hyp Monte Carlo NOEL LOEL TOEL TU PM5D Untransformed C > T Not Run 100 >100 N/A 1 N/A Fisher Exact/Bonferroni-Holm Test Control vs Conc-% TestStat P-Value Decision(O.OS) Lab Control 6.25 1 12.5 0.5 25 1 50 1 100 0.5 Test Acceptability Attribute Test Stat Control Resp 1 Data Summary Conc-% Control Type No-Resp Resp 0 6.25 12.5 25 50 100 Graphics 1.0- 0.9- fl 0.8- S 0> i"* 0.5- S 0.«- 0.3- 0.2- O.t- 0.0 ' Lab Control 10 0 10 0 9 1 10 0 10 0 9 1 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect 1 Non-Significant Effect Acceptability Limits Overlap Decision 0.8 - NL Yes Passes acceptability criteria Total 10 10 10 10 10 10 • « • • • « 0 6.25 12.5 25 Conc-% 50 100 000-089-163-4 CETIS™ v1.6.3revE Analyst'.. CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:39(p1 of 2} 14-7882-2646/0901-3032 Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-7765-6394 Endpoint: Reproduction CETIS Version: CETISvl Analyzed: 13 Feb-09 13:38 Analysis: Nonparametric-Control vs Treatments Official Results: Yes Data Transform Rank Steel Many-One Rank Test Control vs Conc-% Lab Control 6.25 12.5 25 50 100* Test Acceptability Attribute Control Resp PMSD Zeta Alt Hyp OT Monte Carlo Not Run TestStat Critical 91.5 108 110 90 55 75 75 75 75 75 Test Stat Acceptability Ties 6 4 3 2 0 Limits 26 15 -NL 0.218 0.13-0.47 ANOVA Table Source Sum Squares Mean Square DF Between 5289.683 Error 1662.5 Total 6952.183 ANOVA Assumptions Attribute Test 1057.937 30.78704 1088.724 5 54 / 59 Test Stat Variances Mod Levene Equality of Varianc 3.85 Distribution Shapiro-Wilk Normality 0.86 Reproduction Summary Conc-% Control Type 0 Lab Control 6.25 12.5 25 50 100 Rank Transformed Summary Conc-% Control Type 0 Lab Control 6.25 12.5 X 25 50 100 Count 10 10 10 10 10 10 Count 10 10 10 10 10 10 Mean 26 24.8 24.7 26.4 23.4 0 Mean 37.6 32.5 38 39 30.1 6 95% LCL 24.5 23.4 20.6 25 21.5 0 95% LCL 32.8 27.9 30.4 33.9 24.1 6 FStat 34.4 Critical 3.38 95% UCL 27.5 26.2 28.8 27.8 25.3 0 95% UCL 42.4 37 45.5 44 36 6 NOEL 50 P-Value 0.4050 0.8920 0.9150 0.3540 0.0004 LOEL 100 TOEL 70.7 TU 2 .6.3 PMSD 21.8% Decislon(5%) Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Significant Effect Overlap -Decision Yes Yes P-Value 0.0000 Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect P-Value Decision(1%) 0.0047 0.0000 Min 17 16 0 20 15 0 Min 15 14 6 17.5 13 6 Unequal Variances Non-normal Distribution Max 30 30 36 32 31 0 Max 53 53 60 57 55.5 6 Std Err 0.711 0.705 2 0.701 0.931 0 Std Err 2.35 2.21 3.68 2.44 2.89 0 Std Dev 3.83 3.79 10.8 3.78 5.02 0 Std Dev 12.7 11.9 19.8 13.1 15.6 0 CV% 14.7% 15.3% 43.6% 14.3% 21 .4% CV% 33.7% 36.7% 52.2% 33.7% 51.9% 0.0% Diff% 0.0% 4.62% 5.0% -1.54% 10.0% 100.0% Diff% 0.0% 13.7% -0.93% -3.59% 20.1% 84.0% 000-089-163-4 CETIS™ v1.6.3revE Analyst:_QA: CETIS Analytical Report Report Date: Link/Link Code: 13Feb-0913:39(p2of 2) 14-7882-2646/0901-S032 Ceriodaphnla 7-d Survival and Reproduction Test Nautilus Environmental (CA) Analysis No: 17-7765-6394 Endpoint: Reproduction Analyzed: 13 Feb-09 13:38 Analysis: Nonparametric-Control vs Treatments CETIS Version: CETISv1.6.3 Official Results: Yes Graphics §1 I Z(H 10- 6.25 12.5 25 Conc-% SO -40- 100 •2.5 -2.0 -1.5 -1.0 -0.5 0.0 0.5 1.0 1.5 2.0 2.5 Rankits 000-089-163-4 CETIS™ v1.6.3revE Analyst: CETIS Analytical Report Report Date: Link/Link Code Ceriodaphnia 7-d Survival and Reproduction Test Analysis No: Analyzed: 21-3244-7817 Endpoint: Reproduction 13 Feb-09 13:38 Analysis: Linear Interpolation (ICPIN) Linear Interpolation Options X Transform Y Transform Seed Linear Resamples Linear 7747401 280 13 Feb-09 13:39 (p 1 of 1) : 14-7882-2646/0901 -S032 Nautilus Environmental (CA) CETIS Version: CETISv1.6.3 Official Results: Yes Exp 95% CL Method Yes Test Acceptability Attribute TestStat Acceptability Limits Control Resp 26 15 -NL Two-Point Interpolation Overlap Yes Decision Passes acceptability criteria Point Estimates Effect-% Conc-% 95% LCL 95% UCL 50 72.2 67.4 74.9 Reproduction Summary Conc-% 0 6.25 12.5 25 50 100 Control Type Count Lab Control 10 10 10 10 10 10 Mean 26 24.8 24.7 26.4 23.4 0 Min 17 16 0 20 15 0 Calculated Variate Max 30 30 36 32 31 0 StdErr 0.699 0.693 1.97 0.69 0.916 0 Std Dev 3.83 3.79 10.8 3.78 5.02 0 CV% 14.7% 15.3% 43.6% 14.3% 21.4% Diff% 0.0% 4.62% 5.0% -1.54% 10.0% 100.0% Reproduction Detail Conc-% 0 6.25 12.5 25 50 100 Graphics 30- 25- § » ? 15- 10- 5- Control Type Rep 1 Lab Control 17 16 14 20 20 0 k. -•- \ \ Rep 2 24 24 28 26 24 0 \ \ 0 20 40 60 Conc-°/b \ \ 80 Rep 3 30 26 25 27 31 0 \ 100 120 Rep 4 30 30 34 27 21 0 Rep 5 26 29 33 31 29 0 Rep 6 26 23 29 21 15 0 Rep 7 25 24 26 27 27 0 Rep 8 Rep 9 Rep 10 28 29 25 25 26 25 0 36 22 25 32 28 27 20 20 000 000-089-163-4 CETIS ™v1.6.3revE Analyst; CETIS Analytical Report / . , . „ A , n \ X Report Date: 13 Feb-09 13*0 <P 1 of D (UJoCalVXM Vf. CWUcAMH CortHb\ ] Link/Link Code: 1 4-7882-2646/090 1-S032 Ceriodaphnla 7-d Survival and Reproduction Test Analysis No: 19-2626-0178 Endpoint: Reproduction Analyzed: 13 Feb-09 13:39 Analysis: Nonparametric-Two Sample Data Transform Rank Zeta Alt Hyp Monte Carlo NOEL C> T Not Run <0 Wiicoxon Rank Sum Two-Sample Test Control vs Control Test Stat Critical Ties P-Value Lab Control Conductivity Control 62 1 0.0002 Test Acceptability Attribute Control Resp Control Resp PMSD ANOVA Table Source Sum Test Stat Acceptability Limits Overlap 26 15 -NL Yes 17.1 15 -NL Yes 0.177 0.13-0.47 Yes Squares Mean Square DF F Stat P-Value Between 396.05 396.05 1 11.2 0.0036 Error 634.9 35.27222 18 Nautilus Environmental (CA) CETIS Version: CETlSv1.6.3 Official Results: Yes LOEL TOEL TU PMSD 0 N/A 17.7% Decision(5%) Significant Effect Decision Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria Decision(5%) Significant Effect Total 1030.95 431.3222 19 ANOVA Assumptions Attribute Test Test Stat Critical P-Value Variances Variance Ratio F 3.81 6.54 0.0591 Distribution Shapiro-Wilk Normality 0.854 0.0062 Reproduction Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min 0 Lab Control 10 26 24.5 27.5 17 0 Conductivity Co 10 17.1 14.3 19.9 4 Rank Transformed Summary Conc-% Control Type Count Mean 95% LCL 95% UCL Min 0 Lab Control 10 14.8 13.1 16.5 5 0 Conductivity Co 10 6.2 4.83 7.57 1.5 Graphics 40- 5 1 10- &•-*£" Os^ 6- 4- a* -, •6- •8- ' ' ^ Conc-% LC, Decision(1%) Equal Variances Non-normal Distribution Max Std Err Std Dev CV% Diff% 30 0.711 3.83 14.7% 00% 24 1.39 7.48 43.7% 34.2% Max Std Err Std Dev CV% Diff% 19.5 0.817 4.4 29.7% 0.0% 11 0.668 3.6 58.1% 58.1% ^. m 'm I I' • I 1 r- — I — i .0 -1.5 -1.0 -O.S 0.0 0.5 1.0 1.5 2.0 Ranklts 000-089-163-4 CETIS™ v1.6.3revE Analyst Freshwater Chronic Bioassay Client: Dudek & Associates Water Quality Measurements Test Species: C. dubia _ Sample ID: Wei 11 Test No: QqHhl-.5o33- Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Concentration Da PH DO (mg/L) Cond. (pmhos/cm) Temp °C) Lab Control #\M >:*;: &)jM 2H."7. -- •7.4 OO 7.7 •7...^SL id DH DO (mg/L) Tortip (°C) Iff" ' TP fi.M Concentration Da PH DO (mg/L) Cond. ((jmhos/cm) Temp (° Conductivity Control i,,,,,,,! ..»J......l 3200 PH DO (mg/L) Temp (°C) * . initialtr.itf •7,9 743 t.L Concentration Da PH DO (mg/L) Cond. ((jmhos/cm) Tem °C 6.25% aso S-H PH DO (ma/L) Temp fC) HfO -lf\ t-MO -7,97 7.5*8.0 ^flM* :.-....,,. _£J- Concentration Day 12.5% PH DO (mg/L) Cond. ((jmhos/cm) Temp (°C) -16*7oi^o" pH DO (mg/L) Temp (°C) 10 7.ST 35.0 ff.d 25 Analysts: Initial: Final: Sample Used (A, B, C): Comments: Animal Source/Date Received: MO HP /f EF A MO Sample Log-in Numbers: A: QC Check: B: Animal Age at Initiation: — C: "" Final Review:1 ^4 -0 ~<\*~ nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Water Quality (Measurements Client: Dudek & Associates Test Species: C. dubia Sample ID: WelM Test No: Start Date/Time: 1/16/2009 End Date/Time: 1/23/2009 Concentration Da 50% pH DO (mo/L) Cond. (jjmhos/cm) TempJ*Cj 5-3 (0 2fO PH DO (mart.) Temp (*C) 3O£-.l •02- 7.^8 17.57 :rs"7-79- 55-0 ff.f) Concentration Day 100% pH DO (mq/L) Cond. (pmhos/cm) Tem CO Til 7-1 fama). V 6.4% 3,3 9.6 6-69 PH DO (mq/L) Temp (*C) 7.-1 .0 35,0 Comments: _ Animal Source/Date Received: Analysts: Initial: Final: Sample Used (A, 8, C): HO ' , A MO If A Wr typ i* 1/6 J^r>c ^? St- DP A DPXp A 36 /1/^f A . •' ', ^ HD ."i^..../ Thtsma / Sample Log-In Numbers: A: QC Check: Df B: Animal Age at Initiation: C: Final Review: Nautilus Environmental 5550 Morehoust Drive. Suite ISO. San Diego. CA 92121. Freshwater Chronic Bioassay Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna mple 10:Dudek & Associates - Well 1 Start Date/Time: 1/16/2009 ,tjfto Mumber: C^OlOO#,3- End Date/Time: 1/23/2009 \oo<=3 Cone. LC Rep 1 2 3 i 4 ^ 5 6 7 8 9 10 Analyst Verified by: Boar Cur| Jr oT i 12 i '•3 /I %of ^1 L C _33_ J£>bJS Daily Reproduction/ Survival :!:|#:l;£|:! 6. 5 Ao 0 0o pB 0j(^ j;::::j?!:j::i C^ ^) Oo £> 0D O O O W !::i:i:?::::-! O O6 0o o G O 4 O MO :iii|::4:!:::i O 5 tj £/ 4 i4V S" 5t i::;i:|8;:;i;: ti5oetfO <^f-> O O•y 0 X OP1 :|i|::B:!:!l:: lix ^f4~ f\ f\ \<2^ 1-3, 1C '?* 1 1) I 7 \ 3a &l 5o 6///f- ::::::=8j:!:!:; Total n <to .'JCiau d.(j A5^*o gip QC \*f~>^L. Cone. CC Rep 1 2 3 4 5 6 7 8 9 1O Daily Reproduction/ Survival t 1 #!:!(J Qao G r>uoo /7 m$mo o 0noo O V 00 i!;!!|*l:hl O ^D C O O O Oo 0 -Q •;!*:«£;!: CJ* u ^3 ^H•a^ 3I ililllSlilir!; O 0 0 o O D If,0 ^"7 ;:SS?;:i:!:! W'&n \A.(a 1 ey ^& O ~7 V2^ O :-;::;7;:^: """fij"" O if ^«f iO.II '¥6n =i;!;!i?i;!!!!i Total 1 H?'3 i^f Id ataoil OS11 Cone. 6.25% Rep 1 2 3 4 5 6 7^ 8 9 10 ~ — Daily Reproduction/ Survival ' 'Tjljlj; -6>6 0 6ao r> C-, 0& imrnmoof)o 0 0 Vo t)V & Ooo ooooao I!!;!!!*!!!!!! 0 3 T 3 •14r £ <j<- !#i!;5!#;; t* o .2- Ooo(0 13 /DIt Blj!S:!S;i ^' »-•M11 ^c> ^\L», ^f &!i;i7llj:j;i O I? O\<" ifnn Tio CD !H!iH8.-j:!ij;Total ll» M Ito JO ^S3 3*a5 2U>1& Cone. 12.5% Rep 1 2 3 4 5 6 7 8 91o Daily Reproduction/ Survival 1':|:j;i /oao& aa n a SJ ^ mm&f) V <q V G VV flV C? £!:!!&;:!{! O O Ooao<bt»feo l^fi!4ii!Hi ^J 5T <? -<S"sr d/d O 6 #!#S*;# i?O fl 0 o O I/ • — I3u IO lilllliail!!!!! , <1.«? l^ IL ^1C? ""y L^ r/> ;*P*!il;ai ^s? iS »7h&— oo =i:!;li8j|!|ii; — Total (4 ^2^? 3H ^3 ^1 ^U). O/^ JU25- QC £)!«_ QC \ fc7<- QC IH«_ ne Fed/Test Solution Renewed (day): fygo d > tf/s (21 j3co o) 1130 (s> inSo n]\gs& otes: d = dead; M = male; LIP = lost in progress; B = 4th brood (only the first 3 broods are Included in total) •mments: _ Final Review: autilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Client/Sample ID: Dudek & Associates • Well 1 Test Number: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia D. pulex D. magna Start Pate/Time: 1/1 6/2009 End Date/Time: 1/23/2009 Cone. 25% Rep 1 2 3 4 5 6 7 8 9 10 Analyst Verified by: Board/ Cup ± iJ i7 i??3 3*i tf H -13 45 47 $1 TO- & Dally Reproduction/ Survival i;i;;jl3i!i;ij 0 D & ft) 0 & 6 90 J4 ijiiiiSilllj!o O 0 9,0 u o 0 6> u & mmmo o o o o e£> O 0 O U M£> !:&!«;!!!;! 3 t| 4 *6 f 4 *l S7 6 SL !ij!!H*(i!!|i 06 •Q a 5$ 0 '£J{cp i!l!!!*l!i!!! ,?7M1*i,7,y*?•'it 'hJtti^ !i!i!ii?!l!l!! \fi t\ \( i^> 1-7 5 O l>> O O ' W mmm Total 2» A\o AT <JT7 ^.l ai A7 a^> 39. X% QC JSVJA- Cone. 50% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival UlSi! 0 O (0tf a (D O 0 0o mzmoo 0 o p 0 0o'0 t? Hi*!!!!!! Pd oo o <o <D O o £) i|;!i!|^i!i!i! J 5- S"s*<r^5"MH 3 !iii!ii«!!!i!i O 0 "a •s> ?>o /D °( > 7 !{!!i!«l!l!!! fe ^O7, 5 ^&c? ^1w [i!l!!!*!!!!!lri^m^?is CD 1 M O O IHllilSjHli!Total 2.D ^31 ^JiV\ 16 Aljn 10it) QC m*n_ Cone. 100% Rep 1 2 3 4 5 6 7 8 9 10 Daily Reproduction/ Survival ..Jii!6 tf d» 0* ^^7 O c O <J mmmoo Qu r> 0uu $0 !i!i!l!*!!i!!io «D O O c) G CJ 0 <3 <3 i:iil:i^:j!:;j do\o>\<? i o CY^ oo o 0 <s>n ft 0O— C) 0o ft I!!!!!*!!!!!: O (0 £7 /f O — £><? 0c? ;!!!!!!Kl!l!l! 0 (b O o G? • — Oo 0r^ !li?!5!$!l!l!! — Total 0 0 o t> ft - G/*O o fc ^) QC O Time Fed/Test Solution Renewed (day): Notes: d = dead; M = male; LIP 3 lost In progress; B = 4th brood (only the first 3 broods are included In total) Comments: QC Check;Final Review: Nautilus Environmental 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Appendix B Sample Receipt Information Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Sample Check-In Information Client: O 0<K<LK & ArS^eCCCuVe^ Sample ID: Ofc-^^^vVGCxarYN O pfsVveCi UY\ I Sample (A, B, C): Log-in No. (09-xxxx): Sample Collection Date & Time Sample Receipt Date & Time Check-in Temperature (°C) Temperature OK? DO (mg/L) pH (units) Conductivity (uS/cm) Salinity (ppt) Alkalinity (mg/L)* Hardness (mg/L)* * Total Chlorine (mg/L) Technician Initials Freshwater Tests: Control/Dilution Water Source: \8:L\ ( Additional Control? Y N I Dov^sWm -00 1^ l/IS/01 014? */iWo^ ioio \\.7_ <DN q.% "^ %O 3100 \- "25 2-55 >|00° 0,o3 e^ * - mg/L as CaCO3 Mulligan Other Tests Performed] U4\( \ <Or-\cL TestlDNo(s).: Uf,4r^,rv^ -003P l/is/o^ iocs i/tb/cA toio \\.^ (?) N lO.fo 7."7«i •^5/0 2_.2. l<\7 XOoo o-n S- 5 W-ui 3 - ooSi I/1 5/0^ \D30 \/'t/oR IblD \V2- ^0 ^ 5 M, 7.3H H3ZO Z.^ 11-7 >iooo o.o \ £^S f<L*- c,^ V*o^So32- \oso35 \A/a_il I — ooS i. */tS/£fl 10KS i/it/bi 1010 i\.z_ 0 N 5. -7 -72.6 7.G70 I-U Hi >!00<b ^0.050-11 £^ , '= Measured for freshwater samples only, NA = Not Applicable Alkalinity: "?U Hardness: 10 Alkalinity: Hardness: Marine Tests: Control/Dilution Water Source: LABSW ARTSW Other Alkalinity: Salinity: Additional Control? Y N = Sample Salted w/ artificial salt? Y N Sample salted w/brine? Y N Comments: Temperature for sample must be If yes, what ppt? If yes, what ppt? 5 0-6°C if received Alkalinity: Salinity: >24 hours past collection time. Sample Description: , o Airless., ho . CoVor\«-5S., o^QfVe$<i , l 3'.Oflo.au*.,no COC Complete? A B C_ Filtration? _Y_ Pore Size: Organisms or Debris Aeration? _Y_ Length of Final DO: Final pH: pH Adjustment? Y./N, Initial pH: Final pH: CI2 Adjustment? (Y JN Initial Free CI2: STS added: Final Free CI2: B Z-o.i O.iO Sub-samples for additional chemistry: QC Check: tL- 2- Final Review: Itis Appendix C Chain-of-Custody Form CAUFORNIA D WASHINGTON D BR.T.SH COLUMBIA 5550MoreliouseDme. Suite 150 5fl09PDdfcHtfiway&>sr.$(jte2 8664 Commerce Court Choin of Custody Sai Diego, Gftmio 9212) Plione858.587.7333 fox858.587.3961 TocomaWbstaglon 98424 Fhn 253.922.4296 Fox 253.922.581 4 Burnoby British Cabibio Canada VSA 4N7 tone 604.420.8773 fax604.357.1361 Sfm.pl* rfflUctian by-ANALYSES REQUIRED IIReport to: Company Address _ City Contact. Phone/Email SAMPLE ID state C4 zip <f2x>3-f DATE TIME MATRIX Invoice to: Company _ City. Contact State .2P. Phone/Email CONTAKER TYPt NUMtBtOFCONMMERS COAAMENTS JoW)ft PROJEaiNFORMAHON CUENT P.O. NO. to, RELINQUISHED BY (CLIENT)REUNQUISHED BY (COURIER) (Tim*)(Signature) (Printed Name)(Dote)(Printed Name) (Time) (Date) SHIPPED VIA:Y (Company) SKOAL INSTlAJCnONS/eOMMtNiS: . RECEIVED BY (COURIER) (Signature (Company)l0-002.^, 00.^6,1 , oo&Z Additional costs may be required for sample disposal or storage. Net 30 unless otherwise contracted.DISTRIBUTION: WHITE • Nautilus Environmental, COLOR - Originator Glossary of Qualifier Codes: Q1 - Wrong Entry (misspelled, copy error, wrote in wrong spot, etc) Q2 - Temps out of recommended range, refer to CAR Q3 - DO out of recommended range, refer to CAR Q4 - Salinity out of recommended range, refer to CAR Q5 - Miscalculation Q6 - Spilled test chamber/ Lost test animal Q7- Instrumentation Error/Failure Q8 - Inadequate sample volume, 50% renewal Q9 - Inadequate sample volume, no renewal performed Q10 - Sample out of holding time Q11 - Refer to QA section of report for explanation Q12 - Supplemental information is footnoted Q13 - Test initiated with an incorrect number of test organisms Q14 - Replicate(s) not initiated Q15 - Survival counts not recorded due to poor visibility or heavy debris Q16 - Test placed on air due to DO levels dropping below 4.0 mg/L Q17 - Test initiated on air due to an anticipated drop in DO Q18 - Airline obstructed or fell out of replicate and replaced, drop in DO may occur Q19 - Animals initiated out of appropriate age range Q20 - Readings not taken, tech error Q21 - Replicate not counted, tech error Updated 11/12/08