HomeMy WebLinkAboutCUP 194Bx5; Carlsbad Aquafarms; Conditional Use Permit (CUP) (3)INVESTIGATING POTENTIAL SOURCES OF FECAL CONTAMINATION
TO AQUACULTURAL PRODUCTION
IN AQUA HEDIONDA LAGOON
Prepared By.
AGUA HEDIONDA LAGOON
SHELLFISH TECHNICAL ADVISORY COMMITTEE
January 2003
• Page 1«
AGUA HEDIONDA LAGOON SHEOF/SH TECHNICAL ADVISORY CQM/WTTgg REPORT: JANUARY 2003
TABLE OF CONTENTS
TABLE OF CONTENTS .-. 1
introduction 2
Study Design , 5
Replicate Samples 5
Intel-laboratory Quality Control Samples 6
Sample Collection 6
Analytical Methods 7
Results „ 7
Shellfish 8
Waters 9
Interlaboratory Quality Control Samples 10
Tables 1-20 12
Figures 1-4 56
• Page 1 •
AG(JA HEOIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT! JANUARY 2003
INTRODUCTION
The Aqua Hedionda Lagoon Shellfish Technical Advisory Committee (AHLSTAC) is an
advisory body to the Regional Water Quality Control Board (RWQCB) mandated by the
California Shellfish Protection Act of 1993. Its mission is to advise and assist the
RWQCB in developing an investigation and remediation strategy in accordance with
specified law to reduce pollution affecting a threatened shellfish growing area such as
Agua Hedionda Lagoon (AHL), The bill also requires monitoring of the threatened
growing area during the implementation of pollution abatement measures. Members of
the TAC include: Aqua Hedionda Lagoon Foundation, Carlsbad Aquaferms, Inc. (CAI),
San Diego Farm Bureau, Cabrillo Power Company, Cities of Carlsbad, Vista, and
Oceanside, California Department of Health Services (DHS), State Water Resources
Control Board (SWRCB), San Diego Regional Water Quality Control Board, California
Department of Fish and Game, California Coastal Commission, University of California
Cooperative Extension San Diego County Environmental Health Department, and
Audubon Society.
Oysters, clams and mussels are filter-feeding shellfish, taking in large amounts of water
from which they filter and digest planktonic organisms. Thus, they tend to concentrate
waterborne bacteria and viruses in their gastrointestinal tracts. This results in a
potential hearth threat to shellfish consumers if pathogenic microorganisms are present
in high enough concentrations. The DHS regulates the commercial shellfish industry
through a combination of routine water quality monitoring and sanitary surveys.
Sanitary surveys are conducted before a commercial shellfish growing area can begin
operation, and are reevaluated on an annual basis. The purpose of these surveys is to
identify all potential sources of fecal material within the watershed, and to determine if
these sources would present an uncontrollable hazard to the operation of the growing
facility.
According to the National Shellfish Sanitation Program (NSSP), indicator
microorganisms are to be employed for the determination of the presence of fecal
pollution of commercial shellfish growing waters. Conform organisms are the only
officially accepted microbiological indicator group for use in shellfish growing areas.
DHS, as a participant in the NSSP, uses fecal coliform (FC) densities in water samples
as the primary regulatory tool for all commercial shellfish growing areas.
In 1967, Glenn Northcraft, a Carlsbad resident, opened San Diego County's only oyster
farm in the western basin of AHL. The initial sanitary survey concluded that the growing
area in western AHL was Conditionally Approved, meaning that the area met the water
quality standards for an Approved classification (i.e., open harvest conditions) except for
periods of rain when fecal coliform levels were elevated, indicating a potential risk for
the presence of human enteric pathogens from the watershed. Areas that experience
water quality impacts due to rainfall-related runoff are typically closed after a certain
threshold of rain is reached and remain closed for a specified period of time to allow
water and shellfish quality to return to acceptable levels. The oyster farm was later sold
• Page 2 •
AGUA HEDIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
to John Davis, who modified the farm to grow bay mussels and named the business
Carlsbad Aquafarms Inc.
CAI uses large plastic floats to suspend a series of nylon ropes, to each of which are
attached several eight-foot long nylon mesh tubes containing mussels. The plastic
floats used consist of two sizes: small round floats approximately two feet in diameter
and large floats that are horizontally arranged cylinders approximately 2 feet wide by 4
feet long. Anchors hold the float assemblies in place. Even when fully loaded with
mature sets of mussels, the floats are buoyant enough to accommodate one or more
roosting sea birds.
As the mussels grow they fill the tubes, at which point they are harvested and size-
sorted. Sub-market size mussels are returned to the growing area for further growth.
Market-size mussels are placed in a flow-through depuration facility located onshore to
reduce bacteria levels to acceptable levels prior to shipment, Mussel "seed" (juvenile
mussels) is not purchased from a hatchery as there is a sufficient natural set of mussels
within the mussel tubes according to CAI. Depuration is accomplished after the
mussels are held in disinfected filtered seawater for 44 hours. The depuration facility is
certified and overseen by the Department of Health Services' Food and Drug Branch.
Subsequent studies have found that the bacteriological water quality of the lagoon and
shellfish quality has slowly deteriorated over time relative to fecal conform
concentrations. In 1990, based on results of water quality monitoring, the growing
grounds were reclassified as "restricted". As a result, CAI must depurate harvested
mussels in filtered, purified seawater for a minimum of 44 hours before they can be
sold. 1993 legislation mandated that, whenever a commercial shellfish growing area is
defined as threatened, the appropriate Regional Water Quality Control Board shall form
a technical advisory committee to advise and assist the Board in the development of a
remediation strategy. CAI's growing waters meets the legislation's definition of
threatened because the growing classification had been downgraded. The AHL TAG
was convened in 1994 to identify the potential sources of fecal contamination to AHL
and to suggest ways to mitigate the contamination.
Potential contamination sources considered by the AHL TAG include:
• Two wastewater treatment plants, Oceanside and Encina, located north and south of
the lagoon with discharge outfalls into the Pacific Ocean;
• A sewage pump station located east of the growing grounds, where the middle and
outer basins connect;
A main sewer line that is submerged and in direct contact with AHL;
Storm drains that periodically carry runoff from the watershed into AHL;
Migrant encampments;
Recreational users of the lagoon and nearby coastline; and
Feces from warm-blooded animals, including the birds that roost on the aquaculture
structures.
• Page 3 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
The AHL TAG considered the likelihood of each potential source:
• In the recently completed Sanitary Survey by DHS, computer modeling was done on
the ocean outfalls (the Encina Ocean Outfall located south of the lagoon, and the
Oceanside Ocean Outfall located to the north) and their dispersion patterns. Using
available data reported by the wastewater treatment plants, the EPA model
PLUMES suggested that even under worst-case conditions of sewage volume,
bacterial concentration of the sewage, and ocean current speed and direction,
discharge from neither of the outfalls would result in water quality impacts that
exceed the shellfish growing water standard applicable to Agua Hedionda Lagoon.
Modeling was done on the ocean outfalls and their dispersion patterns. The
conclusion of this work was that it is unlikely that fecal contamination from the
outfalls reaches AHL for any extended period of time. However, further study may
be warranted to determine more specifically the extent of the outfalls' contribution to
fecal concentrations in the lagoon;
• Dye tests and flow analyses conducted in the past on the sewage pump station
indicated that there were no major leaks from that system;
• Sewer line damage or spills are required to be reported to the authorities.
Historically, the reported spills do not explain many of the incidences of
contamination in AHL;
• A significant number of contamination events do not coincide with rain events. And
while it is possible to have some storm drains flowing during dry months, the flow to
AHL is minimal;
• Studies have not yet been conducted to determine the potential pollution contribution
from migrant encampments and other human uses; and
• A pilot study to determine the contribution of fecal coliform bacteria (used as an
indicator or fecal contamination) from birds perching on the mussel farm's flotation
system was completed by the San Diego State University Graduate School of Public
Health and the San Diego Regional Water Quality Control Board. This study found
that excluding perching seabirds from much of the mussel farm reduced levels of
fecal coliform in the growing waters, Although coliform levels did not fall to the
concentration required for DHS to reclassify the area, the pilot study indicated that
completely excluding birds from the farm could potentially remove a significant
portion of the bacteria that are contaminating the lagoon.
As a follow-up to the pilot study (funded in part by the Rural Conservation District of
Greater San Diego County), a more detailed study was designed to monitor the growing
area before and after the aquaculture structures were modified with a bird-proofing
system. After several sampling events, it became clear that the bird-proofing measures
as designed were ineffective. CAI determined that twice as many spikes would be
needed on the floating structures to adequately exclude the larger roosting birds. The
TAC voted to modify the study design and seek additional funding before completing the
study. The State Water Resources Control Board agreed to fund the work presented
below, and requested that the Rural Conservation District contract with the Board in
order to make the money available to complete the study.
AGfJA HEDIQNDA tftGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
The purpose of the current study was to determine the impact to water and shellfish
quality in the commercial growing area in outer Agua Hedionda Lagoon (AHL) by birds
that roost on the aquaculture structures. The study design called for two distinct growing
areas spatially separated. One area was the current location of CAI's mussel lines: all
previous bird-proofing was removed from these structures. The second location was
placed along the western shoreline of the outer lagoon (Figure 1). It was initially desired
to place the bird-free location at the north end of the outer lagoon but CAI determined
that this was not feasible. The mussel line structures located along the western
shoreline were bird-proofed by attaching numerous plastic spikes to the exposed
surface of the floats. These spikes remained in place for the duration of the study.
These two locations were sampled concurrently in an attempt to determine if there was
a difference in fecal coliform concentrations in shellfish or waters for these two
treatments. In addition, several other locations in the outer lagoon were established as
auxiliary water quality sampling stations,
STUDY DESIGN
The study consisted of three sampling events. Each sampling event consisted of three
consecutive days of sampling. On each sampling day, water samples were collected at
11 water quality stations: three within the current growing area, two within the new
growing area, and seven between and around the two growing areas (Table 1, Figure
1). Mussels were collected from five shellfish stations within the two growing areas. An
additional sampling station was added for the third event. A buoy located at the north
end of the outer lagoon, which was populated with a natural set of bay mussels (Mytilus
edulis), was designated for sampling of both shellfish and waters for all three days of
the final sampling event. A summary of sample allocation per sampling day is provided
in Table 2a and Table 2b.
All samples were analyzed for fecal coliform. Sampling events were scheduled to occur
when the bird population roosting in the lagoon was sufficiently large, a determination
that was based on historic bird counts provided by the Audubon Society. Bird counts
and location were recorded for several days over at least a one week period prior to the
first sampling event to confirm that the new growing area is consistently free of birds.
Bird counts and location were also recorded in the evening before each sampling day,
and during sampling.
REPLICATE SAMPLES
Duplicate water samples were collected at each of the 7 stations outside of the growing
areas (i.e., stations 2, 4, 6, 7, 40,41, and 42). Four replicate water samples were
collected at each of the 2 stations in the new growing area (W1 and W2) and at each of
the 3 stations in the existing growing area (5, 5N, and 53). Four replicate mussel
samples were collected at each of the 5 shellfish stations within the two growing areas
on each day (stations # 5, 5N, 5S, W1, and W2). All replicate sample results were used
Page 5 *
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
for the statistical analyses. Values greater the upper limit of detection (e.g., >= 160,000
MPN) were assumed to be just at this upper limit (i.e., 160,000 MPN), introducing the
possibility that the sample results exceeding the detection limit are underestimates.
Likewise, sample results below the detection limit (e.g, < 20 MPN) were assumed to be
just below that level (i.e., 20 MPN) and may therefore represent an overestimate.
INTERLABORATORY QUALITY CONTROL SAMPLES
All shellfish samples were analyzed by a local certified laboratory, Motile Laboratory,
which will be referred to in this report as the "primary shellfish lab". All water samples
were shipped to another certified lab (Biovir Laboratory;Table 3) in the bay area,
heretofore designated as the "primary water lab". Interlaboratory quality control (QC)
samples were collected for both shellfish and water samples. A laboratory duplicate
water and shellfish sample were collected from each of three randomly selected
sampling stations on each day. In an effort to ensure that a homogenous water sample
is sent to each laboratory, a 250 mL bottle was used to collect the water sample at each
of these three stations. Two 100 mL sample bottles were filled from the 250 ml bottle,
with one bottle going to each of the two laboratories involved in the study. The shellfish
QC samples were shipped to the primary water lab and the water QC samples were
delivered to the primary shellfish lab.
SAMPLE COLLECTION
Attempts were made to conduct sampling at slack tide during the morning. This
approach was intended to minimize the impact from any outside sources due to tidal
transport and to maximize the chances of detecting an impact from birds, the majority of
which roosted overnight and left the area during the day. Unfortunately it was not
possible to complete all of the sampling during slack tide due to constraints caused by
the time required for two persons to complete the sampling (approximately three hours)
and the need to sample at the appropriate time to meet the 24-hour hold times for those
sample shipped to a laboratory located in northern California. In general, shellfish
samples were collected first, followed by collection of water samples.
All water samples were collected at a depth of 1 foot below the surface. Water samples
were collected in sterile 100 milliliter (mL) screw-cap polypropylene bottles or the
equivalent. All bottles were immediately placed in an insulated ice chest containing
sufficient ice packs ("blue ice") to maintain a temperature between 4° and 10° C. A
temperature control blank was collected at the start of the sampling day and stored in
the ice chest with the other water samples. Samples were transported or shipped to the
appropriate laboratory, where all analyses were performed within the holding times
required under the NSSP. The laboratories immediately checked the temperature of the
control blank at the time of receipt to ensure that proper holding temperatures were
maintained during shipment.
> Page 6 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL AQVISORY COMMITTEE REPORT: JANUARY 2003
Shellfish samples were collected from the mussel lines at arms-length below the surface
(i.e., approximately 2 to 4 feet below the surface of the water). Each sample consisted
of a minimum of 15 individuals, more if necessary, to ensure that a minimum of 250
grams of tissue was available to the laboratory. Collection of replicate samples at a site
was accomplished by collecting a large pool of shellfish from which subsamples were
randomly drawn for preparing the replicate samples.
Mussels were immediately placed into plastic bags, which were then labeled with the
sampling location, date, and time and sealed to ensure that there was no commingling
of samples. Samples were then immediately placed in an insulated ice chest containing
sufficient ice packs ("blue ice") to maintain a temperature between 4° and 10° C prior to
delivery to the laboratory.
Field data forms were completed as samples were collected. The information recorded
included the sampling date and time, sample identification number, location, water
temperature and salinity, and any miscellaneous comments on field conditions.
On the evening before each sampling day a bird count was conducted to determine the
number and location of birds in the growing area. Numbers of each species and
approximate locations within the growing area were recorded.
ANALYTICAL METHODS
All water samples were analyzed for fecal coliform (FC) using the most probable
numbers (MPN) estimate of bacterial density in a multiple tube fermentation test
(Standard Methods, 18th ed., Part 9221,1992). The reporting units for this method are
most probable number per 100 milliliters (MPN/100 mL) for water and MPN/100 grams
for shellfish samples.
Shellfish samples were analyzed for FC using a most probable number estimate of
bacterial density in a multiple tube fermentation test (American Public Health
Association, 1970; Standard Methods, 18th ed., Part 9215 A and B, 1992). Following a
review of the results of the first sampling event, in which several samples exceeded the
upper detection limit for the assay (reported as £16,000 MPN), the AHLSTAC decided
to request the laboratories to add an additional dilution to the shellfish assay. This step
increased the upper detection limit by one log factor to 160,000 MPN.
RESULTS
Bird counts were conducted the week prior to the first sampling event on September 25-
27. The number of birds ranged from 824 to 1063 and consisted mostly of gulls and
pelicans (Table 4, Figure 2). The number of birds remained relatively consistent
throughout the sampling events, ranging from 711 to 1191 (Tables 5-8). Sampling
Page 7
AGUA H5DIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
events were conducted on October 2-4, October 15-17, and November 5-17, 2001.
There were no birds observed in the "no-bird" area when bird counts were made.
Shellfish
In general there was a great deal of variation in shellfish fecal coliform concentrations
from event to event and often from day to day (Table 9). During Event 1 the fecal
coliform concentrations ranged from 230 MPN (the market standard for fecal coliform in
shellfish) to £16,000 MPN, the upper detection limit for the standard assay. Several
samples from the bird-occupied area exceeded the upper detection limit (16,000 MPN)
for fecal coliform concentrations on Day 3 of Event 1. In an attempt to ensure an
accurate estimate of these extremely elevated concentrations of fecal coliform, the
AHLSTAC modified the analytical protocol by including an additional dilution to raise the
upper detection limit to 160,000 MPN. Unfortunately several shellfish samples collected
during subsequent sampling events exceeded this upper limit as well. Samples from the
bird- occupied area exceeded this upper limit of 160,000 MPN on Day 2 of Event 2 and
on Day 2 of Event 3.
The fecal coliform geometric means for mussels from the stations within the bird-free
area ranged from 272 MPN to 3131 MPN over the course of the study (Table 10). The
overall fecal coliform geometric mean for the bird-free area was 956 MPN. The fecal
coliform geometric means for mussels from the bird-occupied area ranged from 1183
MPN to 7766 MPN. The fecal coliform geometric mean for shellfish from the buoy site
during Event 3 was 194 MPN. This location was the only site that met the shellfish
market standard. The overall fecal coliform geometric mean for the bird-occupied area
was 2619 MPN. For each event the geometric mean for each bird-free site was always
lower than that of the bird-occupied sites, with the exception of site #5 during Event 2
(Table 11). The 90% confidence intervals of the bird-free and bird-occupied sites
overlap to varying degrees during all events except Event 3, where the upper
confidence interval of the bird-free sites did not overlap the lower confidence interval of
the bird-occupied sites. When the data for all events is combined, the 90% confidence
intervals of the bird-occupied and bird-free areas do not overlap (Table 11).
Statistical tests for normality of the data were performed (Kolmogorov-Smirnov,
Sharpiro-Wilks) using SPSS statistical software. The raw fecal coliform data were
determined not to be normal (p < 0.002), therefore all fecal coliform data was
transformed to their base 10 logarithms. There was an improvement in normality when
the log-transformed data was analyzed, however some data remained non-normal so
subsequent statistical analyses were conducted with nonparametric tests.
The shellfish fecal coliform data were examined for significant differences among all
sites. The results of the Wilcoxson Rank Sums test demonstrated three major groupings
of ranks: the sites from the bird-occupied area occupied the uppermost ranks, followed
by the sites in the bird-free area and finally the buoy site (Table 12). Prior to comparing
the group of sites from the bird-occupied area with the group of sites from the bird-free
AGUA HZQIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
area it was necessary to determine if the variability among each group would preclude
such an analysis. The results of a Kruskal-Wallis analysis showed that there were no
significant differences among the bird-occupied sites (Table 13). Therefore the data
from the bird-occupied sites was grouped and compared with the grouped data from the
bird-free area. The results of this analysis showed that there was a significant difference
between the bird-occupied area and the bird-free area (p < 0.0001). Therefore the
mussel samples from the bird-occupied area contained significantly higher fecal coliform
concentrations than the bird-free area, With the exception of Day 3 of Event 2 the fecal
coliform concentrations were always highest in the mussels from the bird-occupied area
compared to mussels from the bird-free area or the buoy (Figure 3). It should be pointed
out that the statistical analyses were run on the data set both with and without the buoy
data. The results of these analyses were the same, therefore the use of data from the
buoy site did not skew the statistical results (Table 14).
Waters
Fecal coliform concentrations in water samples ranged from <1.8 MPN to 260 MPN in
the bird- occupied area and from <1.8 MPN to 70 MPN in the bird-free area (Table 15).
Fecal coliform concentrations in water samples from the buoy site ranged from <1.8 to
13 MPN. The fecal coliform concentrations for the remainder of the water quality
stations ranged from <1.8 MPN to 130 MPN, with the highest concentrations detected at
stations 4 and 6.
The fecal coliform geometric means for water samples from the stations within the bird-
free area ranged from 4 MPN to 19 MPN over the course of the study (Table 16). The
overall fecal coliform geometric mean for water samples from the bird-free area was 8.5
MPN. The fecal coliform geometric means for water samples from the bird-occupied
area ranged from 3 MPN to 62 MPN. The overall fecal coliform geometric mean for
water samples from the bird-occupied area was 10.2 MPN, It appears that the
geometric means of the bird-occupied and bird-free sites are generally higher in fecal
coliform than the bird-free sites, with the highest overall fecal coliform concentrations
detected at site #5S (Figure 4).
Statistical tests for normality of the data demonstrated that the raw fecal coliform data
was not normal (p < 0.002). The fecal coliform data was transformed (base 10
logarithms), resulting in a noticeable improvement in normality when the log-
transformed data was analyzed. However some data remained non-normal so
subsequent statistical analyses were conducted with nonparametric tests.
The water sample fecal coliform data were examined for significant differences among
all sites. The Kruskal-Wallis Non-Parametric Rank Test demonstrated a significant
difference among sites for fecal coliform concentrations in water (p < 0.000).These
mean ranks show that the fecal coliform concentrations in water samples from site #05S
in the bird-occupied area have a much higher ranking than the other sites in this area or
in the bird-free area (Table 17a). The remainder of the sites demonstrate no strong
• Page 9 •
AGUA HeOtQNQA iAGQO/V SHELLFISH TECHNICAL AQVISQRY COMMITTEE REPORT: JANUARY 2003
pattern in rankings as was observed for the shellfish fecal coltform data. Based on the
mean ranks, it appeared that the higher ranking of station #05S may be the cause of
this finding of significance. The data from this site were removed and the data
reanalyze. The results showed no significant difference among the remaining sites
(Table 17b). It should be noted that the data from the buoy station were not included in
any of these analyses: the low ranking of the buoy data would produce a significant
result whether or not site #05S was included.
Prior to comparing the group of sites from the bird-occupied area with the group of sites
from the bird-free area it was necessary to determine if the variability among each group
would preclude such an analysis. The results of a Kruskal-Wallis analysis showed that
there were differences within the bird-occupied sites and within the bird-free sites (p <
0.01). Despite the differences within each of the two groups of sites, the data from each
group was compared to the other using the Mann-Whitney non-parametric test. The
result of this non-parametric test indicated that there was no discernable difference
between the water samples of the bird-occupied area and those of the bird-free area
(p=0.118;Table18).
Interlaboratory Quality Control Samples
Each QC sample was compared to the geometric mean and confidence intervals of the
remaining replicates. For example, five replicate shellfish samples were collected at the
designated QC stations each day. Four of the replicates were analyzed at the primary
laboratory and the fifth replicate was shipped to the secondary laboratory for assay. The
results of the replicates from the primary laboratory were used to calculate a geometric
mean and the upper and lower confidence limits at = .10 (i.e., 90%). In addition, these
data were compared to the estimated 95% confidence intervals for MPN values from the
U.S, Food and Drug Administration's (FDA) Bacteriological Analytical Manual1. The
same process was followed with the water QC samples with two exceptions: (1) the
number of replicates varied according to the site; and (2) the replicate water samples
were shipped to the primary lab while the QC water sample was delivered to the
secondary water lab on site. The need to ship samples created a disparity because of
the lengthy holding time involved compared to the short holding times of the on-site
primary shellfish laboratory. As a result the samples do not serve as rigorous
interlaboratory QC samples due to different holding times prior to the start of the
assays.
In general, there was poor agreement between laboratories for the assay results of split
shellfish and water samples. Of the 27 shellfish QC samples sent to the secondary
shellfish lab, 18 did not fall within either their respective calculated 90% confidence
interval or the FDA's estimated 95% confidence intervals for fecal coliform MPN. Of
these 18 samples, 17 had lower fecal coliform concentrations than the respective values
from the primary shellfish laboratory (Table 19). The calculated 90% confidence
1 8m Edition (Revision A), 1998.
• Page 10 •
AGUA HEDiONOA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
intervals for each set of replicates were usually well within the FDA's estimated 95%
confidence intervals, indicating an acceptable level of precision for these assays.
Of the 18 water QC samples delivered to the secondary water lab, 11 did not fall within
either their respective calculated 90% confidence interval or the FDA's estimated 95%
confidence intervals for fecal coliform MPN, All 11 of these samples had higher fecal
coliform concentrations than the respective values from the primary water laboratory
(Table 20).
Although there was poor agreement between laboratories, there was a pattern in which
results from the laboratory capable of analyzing samples within a short holding time
were consistently higher. Unfortunately it is not possible to determine if this observed
pattern was related to bacterial die-off as a result of a greater lag time between sample
collection and microbiological assay. However, this possibility should be considered in
future studies involving more than one laboratory.
' Page 11 >
AGUA HEQIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
TABLES 1 - 20.
Page 12 <
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 1. Description of sampling locations.
Water Log boom at Cannon Lake outlet
6 Water Railroad trestle at outlet from middle lagoon
Water Mouth of Agua Hedionda Lagoon, east of road
overpass
40 Water Western location between new and existing growing
areas
41 Water Midway between new and existing growing areas
42 Water Eastern location between new and existing growing
areas
5N Water. Shellfish North end of existing growing area
Water, Shellfish Center of growing area
5S Water, Shellfish South end of existing growing area
WN1 Water. Shellfish New growing area in north lagoon, north end
WN2 Water, Shellfish New growing area in north lagoon, south end
Buoy Water, Shellfish Northwest end of outer lagoon, in main channel
" Added for third sampling ev$nt
• Page 13 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Table 2a. Allocation and number of base samples, field quality assurance samples, and
laboratory quality assurance samples for each day of the study period.
40
41
42
5N
58
WN1
WS1
Subtotal 11 21 15
TOTAL 35 23
Table 2b. Allocation and number of additional samples for the buoy station, which was
added to Event 3, for each day of the study period.
' 3 of the 5 growing area stations are randomly selected each day
• Page 14 *
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Table 3. List of sample types, number of samples, and designated laboratories for
analysis of samples.
3 randomly selected growing area
stations
All mussel sampling stations (4 @
each of 5 stations)
All non-growing area water sampling
stations (2 @ each of 6 stations)
TOTAL PER DAY
3 waters"3 mussel
samples
20 mussel samples 20 waters
12 waters
20 mussel samples 3 mussel samples
3 waters 32 waters
a) Total number of shellfish samples per event and per study:
23 mussel samples x 3 days = 69 mussel samples per event
69 mussel samples per event x 3 events - 207 mussel samples
b) Total number of water samples per event and per study.
35 water samples x 3 days = 105 water samples per event
105 water samples per event x 3 events = 315 water samples
4 Split sample of a 250 mL sample; the other half used as the interlaboratory QC sample analyzed by Biovir Labs.6 Each mussel sample contains at least 15 individual mussels, to ensure at least 250 grams of tissue.
1 Page 15 <
AGUA HEDSONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 4. Preliminary bird counts conducted on September 25-27, 2001, prior to the first sampling event.
DATE: 09>26JW
START TIME: 8:45p«n
END TIME: 8:4Spm
COUNTER: Pat & Grata Efiiott
RAFT
1
2
3
4
5
6
7
6
9
10
11
12
13
14
15
16
17
1£
19
2C
21
GULL
Hole: 1.2
50
15
25
2
1
3
7
5
t
20
10
10
30
25
15
200
160
260
:>ELICAN CORMORANT
OTHEF
Jirrfs spooked
3
r
1
14
15
23
!5
17
30
20
10
?
\
0
10
10
i
1
1
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
3
r
3
3
10
U
t2
13
14
15
16
17
18
19
20
21
3ULL
30
JO
20
20
20
10
15
10
5
2
,
j
4
15
20
25
25
20
ISO
100
240
D9B6KI1
8:3Cpm
9:30pm
PataCraigEIioH
PELl
CAN
4
20
15
12
15
15
10
25
3
Z
1
2
1
4
3
0
0
>
35
20
2
:ORMORANT
OTHER
1
3ATE:
START TIME:
END TIME:
HUNTER:
WFT
1
2
3
1
5
S
r
9
3
10
11
12
13
\4
15
16
17
IS
19
20
21
GUU
87
25
0
(
5
40
11
JO
13
7
S
&
10
36
35
27
25
11
75
90
100
marai
8:30pm
9:30pm
Pat&CraiqBSoii
Bob&Diane Richards
3ELI
3AN
17
12
37
30
40
15
12
16
>
3
5
,
1
2
3
3
1
3
4
3
)
CORMORANT
OTHER
• Page 16 •
AGVA HEOIOftDA LAGOON SHELLFISH TECHNICAL ADWSORY COMMITTEE REPORT JANUARY 2Q03
k-[TOTAL 199 h[TOTAL
o t
793 |l92
I
i
NOTES:NOTES:MOTES:
Appro* SO guls in watei west of 18.Other great blue heron
912 tujls total
betwesn 14&15. Foggy. Many
. 150 to 200. few before being
counted.
1113 birds total.
• Page 17 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Table 5. Bird counts conducted during the first sampling event of October 2-4, 2001.
DATE: 10/01/01
START TIME: 8:30pm
END TIME: 9:30pm
COUNTER: Bob Richards
RAFT
1
2
3
4
5
6
7
B
9
10
11
12
13
14
15
16
17
18
19
20
21
GULL
25
B
9
3
4
53
7
30
29
5
15
21
21
26
35
23
28
21
103
30
83
PELI
CAN
4
14
25
18
18
9
16
21
18
27
10
5
5
1
0
0
0
0
0
3
0
CORMORAN1
OTHER
DATE:
START TIME:
END TIME:
COUNTER:
=?AFT
1
2
3
4
5
5
7
3
3
10
11
12
13
14
15
16
17
18
13
20
21
GULL
55
30
12
5
5
12
30
16
16
10
3
1
11
10/02/01
8:40pm
9:45pm
Pat & CraFg Elliott
3ELI
CAN
22
11
20
25
23
32
30
30
16
36
20
16
21
CORMORANT
OTHER
1
14-18 combined. See note.
130
170
120
250
30
20
4
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
5
1
B
9
10
11
12
13
14
15
16
17
18
19
20
21
GULL
0
1
6
10
4
12
20
13
8
5
7
0
7
0
8
22
21
30
120
120
240
10/03/01
8: 35pm
9:45pm
Pat & Craig Elliott
PELI
CAN
12
11
1
J
1
40
45
33
38
50
36
»3
43
53
28
3
2
1
60
20
5
CORMORANT
OTHER
Page 181
AGUA HEDtONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
OTAL 1196 OTAL 1881 56
22
TOTAL 136
NOTES:
Clear weather. Birds calm.
NOTES: Steam release (blast) from
power plant for two or three
minutes spooked gulls. Number
shown is estimate.
NOTES; Many pelicans flew off when
spot light shown on first four rafts.
Ten gulls in water around 13.
1QO gulls in water between 14 & 19.
Total gull probably 765.
• Page 19 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPQRr JANUARY 2003
Table 6. Bird counts conducted during the second sampling event of October 15-17, 2001.
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
5
1
B
9
10
11
12
13
14
15
16
17
18
19
20
21
GULL
GO
25
3
3
3
0
3
15
5
10
20
20
10
20
20
30
20
25
40
70
240
10/14701
8:45pm
9: 30pm
Pat & Craig Elliott
=>ELI
CAN
3
4
D
0
0
50
40
15
20
10
10
10
10
5
0
0
0
0
120
70
5
CORMORANT
OTHER
1
1
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
6
p
B
9
10
11
12
13
14
15
16
17
18
13
20
21
GULL
55
30
20
15
20
10
4
13
4
5
9
8
15
20
20
40
45
40
40
40
20
10/15/01
8:35pm
9: 25pm
Pat & Craig Elliott
PELI
CAN
3
1
5
0
3
35
60
31
28
13
10
12
D
&
0
0
0
0
10
5
0
CORMORANT
1
OTHER
1
2
1
1
20
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
3
7
3
3
10
11
lj\2
13
14
15
16
17
18
19
20
21
GULL
4
5
13
14
11
10
5
18
5
5
10
10
10
15
20
20
30
15
45
70
200
10/16/01
8: 30pm
9: 10pm
Pat & Craig Elliott
PELI
CAN
0
0
0
1
2
35
50
30
25
10
10
10
10
2
2
0
5
3
110
70
0
CORMORANT
OTHER
20
• Page 20 •
AGUA HEDSONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
22 1
TOTAL (642 38S
1
t
bz b jo
[TOTAL 1*73 J219
I
U
b b b
(TOTAL jsas b?8
I
bo
NOTES:NOTES:NOTES:
Other Great Blue Heron. Rafts 1-4 spooked
after finish count on 1. Raft 2-4 estimate.
Loud seals. Rafts 19-21 spooked by light,
figures are estimates.
Seals still barking. Rafts 19-21 spooked. Unable
to make good estimate of departing peii'cans,
probably as many as previous night.
Others: Rafts 3-9 Great Slue Heron. Rafts 10-21
Activity at Aquafarm dock may have discouraged
bird presence on rafts 1-4. Activity associated
with sewer spill in lagoon earlier in the day. We
were informed by guard of spill. Seals still around.
• Page 21 •
AGUA HEDSONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMfTTEE REPORT; JANUARY 2003
Table 7. Bird counts conducted during the third sampling event of November 5-7, 2001.
DATE:
START" TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
5
rt3
7
3
3
10
11
12
13
14
15
16
17
18
19
20
21
GULL
3
9
18
B
2 __,
5
1
2
10
10
29
12
28
48
22
18
25
20
70
35
15
11/04/01
7:30pm
8:20pm
Pat & Craig Elliott
PELI
CAN
0
D
1
5
4
32
ro
50
21
10
4
9
19
3
4
9
2
1
50
10
0
CORMORANT
OTHER
2
B
5
1
3
DATE:
START TIME:
EN DTI ME:
COUNTER:
RAFT
1
2
3
4
5
B
7
S
9
10
11
12
13
14
15
16
17
18
19
20
21
GULL "
1
2
1
3
1
0
0
1
5
5
30
19
32
14
18
35
21
23
150
120
200
11/05/01
7:30pm
8: 15pm
Craig Elliott &
Bob Richards
PELI CAN
D
0
3
5
5
1
5
29
15
22
10
9
7
12
11
4
0
0
20
20
5
CORMORANT
OTHER
2
3
10
DATE:
START TIME:
END TIME:
COUNTER:
RAFT
1
2
3
4
S
7
3
9
10
11
12
13
14
15
16
17
18
19
20
21
GULL
,
60
/
22
2
1
1
4
7
11
14
12
19
31
24
22
42
57
20
120
200
11/06/01
7: 30pm
8:15pm
Craig Elliott &
Bob Richards
=>ELf
CAN
5
D
0
4
29
30
34
37
14
19
2
2
2
4
10
12
0
1
100
40
50
CORMORANT OTHER
1
Page 221
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
22
TOTA
L.
0
388
408
D
304 19
22
TOTAL
D
581
711
0
182 15
22
[TOTA
L
0
sag
0
420 1
NOTES: Approx 20 gulls spooked from 1 thru 4
when light shown.
Seal barking. Lots of lightening.
Floats in rafts 20 & 21 mostly underwater.
Others are egrets.
NOTES: Approx 30 gulls spooked from 1 thru 4
when light shown.
Floats in rafts 20 & 21 above water again
20-21 near)/ full. Numbers are estimates
since birds spooked.
NOTES: Approx 80 gufis spooked from 1 thru 4
when light shown.
20-21 full. Numbers are estimates since birds
spooked.
Other great blue heron.
Page 23 *
AGUA HEDtONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
Table 8. Total bird counts for each day of the study.
711 B78 11101063 086 \824 B29 11235 1191 1029 [718
' Page 24 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMiTFEE REPORT. JANUARY 2003
Table 9. Fecal coliform results for shellfish samples from all sampling events.
AH-0417-02 10/02/01 1255 Agua Hedionda. WQ Station #05N CBMU 230
AH-0417-03 10/02/01 1255 Agua Hedionda. WQ Station #05N CBMU 1300
AH-0417-04 10/02/01 1255 Agua Hedionda. WQ Station #05N CBMU 230
AH-0417-05 10/02/01 1310 Agua Hedionda. WQ Station #05 CBMU 230 499.4
AH-Q4t7-06 10/02/01 1310 Agua Hedionda. WQ Station #05 CBMU 2400
AH-0417-07 10/02/01 1310 Agua Hedionda. WQ Station #05 CBMU 230
AH-0417-08 10/02/01 1310 Agua Hedionda, WQ Station #05 CBMU 490
AH-0417-09 10/02/01 1345 Agua Hediortda. WQ Station #OSS CBMU 2400 2400.0
AH-0417-10 10/02/01 1345 Agua HedtorKJa. WQ Station #05S CBMU 2400
AH-0417-11 10/02/01 1345 Agua Hedforxia, WQ Station #053 CBMU 2400
AH-0417-12 10/02/01 1345 Agua Hedionda, WQ Station #05S CBMU 2400
AK-0417-13 10/02/01 1405 Agua Hedionda. WQ Station #WN1 CBMU 230 637.4
1AH-0417-14 10/02/01 1405 Agua Hedionda. WQ Station #WN1 CBMU 230
AH-0417-15 10/02/01 1405 Agua Hedionda. WQ Station #WN1 CBMU 2400
AH-0417-16 10/02/01 1405 Agua Hedionda, WQ Station #WN1 CBMU 1300
AH-0417-17 10/02/01 1430 Agua Hedionda, WQ Station #WS1 CBMU 1300 637.4
AH-0417-18 10/02/01 1430 Agua Hedionda. WQ Station #WS1 CBMU 230
AH-0417-19 10/02/01 1430 Agua Hedionda, WQ Station #WS1 CBMU 2400
AH-0417-20 10/02/01 1430 Agua Hedionda. WQ Station #WS1 CBMU 230
Event 1, Day 2:
AH-0418-01 10/03/01 0930 Agua Hedionda. WQ Station #05N CBMU 230 488.7
AH-0418-02 10/03/01 0930 Agua Hedionda, WQ Station #C5N CBMU 2200
AH-0418-03 10/03/01 0930 Agua Hedionda, WQ Station #C5N CBMU 230
AH-0418-04 10/03/01 0930 Agua Hedionda, WQ Station #05N CBMU 490
AH-0418-05 10/03/01 1000 Agua Hedionda, WQ Station #05 CBMU 2400 1335.3
! CBMU = Cultured Bay Mussel
Page 25'
AGUA HEDKSNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
AH-0418-06
AH-04 18-07
AH-04 18-08
AH-O4 18-09
AH-0418-10
AH-0418-11
AH-0418-12
AH-0418-13
AH-Q418-14
AH-0418-15
AH-0418-16
AH-0418-17
AH-0418-18
AH-Q418-19
AH-04 18-20
Event 1, Day 3:
AH-04 19-01
AH-04 19-02
AH-04 19-03
AH-04 19-04
AH-04 19-05
AH ^04 19-06
AH ^04 19-07
AH-0419-08
AH-0419-09
AH-0419-10
AH-0419-11
AH-0419-12
AH-0419-13
AH-0419-14
AH-0419-15
AH-0419-16
AH-Q419-17
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/D1
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
1000
1000
1000
1020
1020
1020
1020
1050
1050
1050
1050
1115
1115
1115
1115
0900
0900
0900
0900
0925
0925
0925
0925
0950
0950
0950
0950
1015
1015
1015
1015
1030
Agua Hedionda, WQ Station #05
Aqua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda. WQ Station #05
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
>
>
>
2400
2400
230
2400
1300
1600
2400
490
490
490
490
230
230
230
230
1300
16000
16000
2400
2400
2400
1300
16000
1300
230
1300
2400
5400
2400
2400
2400
2400
1860.5
490.0
230.0
5316.2
3308.4
982.8
2939.4
2400.0!
Page 26 •
AGUA H£D!ONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
AH-0419-18
AH-0419-19
AH-0419-20
Event2, Day.1!::-:';' i •::
AH-0423-01
AK-0423-02
AH-0423-03
AH-0423-04
AH-0423-05
AH-0423-06
AH-0423-07
AH-0423-08
AH-0423-09
AH-0423-10
AH-0423-11
AH-0423-12
AH-0423-13
AH-0423-14
AH-0423-15
AH-0423-16
AH-0423-17
AH-0423-18
AH-0423-19
AH-0423-20
Event 2. Day 2:
AH-0424-01
AH-0424-02
AH-0424^03
AH-0424^04
AH-0424-05
AH-0424-06
AH-0424-07
10/04/01
1 (MM/01
10/04/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/16/01
10/16/01
10)16/01
10/16/01
10/16/01
10/16/01
10/15/01
1030
1030
1030
1145
1145
1145
1145
1200
1200
1200
1200
1215
1215
1215
1215
1230
1230
1230
1230
1250
1250
1250
1250
1005
1005
1005
1005
1035
1035
1035
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
. : ! •- . . : : : .: - . : : " '.:',::• : : :
• .. ' . . ' . . . : : : : • . ~: . :
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #C5N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #Q5S
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Stalion #WN1
Ague Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
<
<
<
<
<
<
<
<
<
<
<
<
<
>
>
>
2400
2400
2400
; ;•;
180
450
160
180
180
180
180
180
1300
4900
2300
450
180
180
180
180
450
180
450
180
160000
160000
24000
160000
4900
2300
2300
;;•;;'; i^:; .| ;
r 226.3
180.0
1602.4
180.0
284.6
99573.3
2778.7
• Page 27 •
AGUA HEDtONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
AH-0424-08
AH-0424-09
AH-0424-1Q
AH-0424-11
AH-0424-12
AH-0424-13
AH-0424-14
AH-0424-15
AHO424-16
AH-0424-17
AH-0424-18
AH-0424-19
AH-0424-20
Event 2, Day 3: :
AH-0425-01
AH-0425-02
AH-0425-03
AH-0425-04
AH-0425-05
AH-0425-06
AH-0425-07
AH -0425-08
AH-0425-09
AH-0425-10
AH-0425-11
AH-0425-12
AH-0425-13
AH-0425-14
AH4J425-15
AH-0425-16
AH-O425-17
AH-0425-18
AH-0425-19
10/16/01
10/16/01
10/16/01
1035
1050
1050
10/16/01 | 1050
10/16/01 | 1050
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
1115
1115
1115
1115
1135
1135
1135
1135
0945
0945
0945
0945
1000
1000
1000
1000
1015
1015
1015
1015
1030
1030
1030
1030
1045
1045
1045
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda. WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda. WQ Station #WS1
Agua Hedionda. WQ Station #WS1
Agua Hedionda, WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua Hedionda. WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda. WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda. WQ Station #WS1
Agua Hedionda. WQ Station #WS1
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
>
2300
22000
54000
2300
160000
4900
13000
2300
13000
22000
13000
4900
9500
13000
930
2300
2300
2300
2300
4900
7600
4900
13000
2300
4300
35000
24000
22000
24000
4300
9500
13000
25713.S
6606.2
10741.7
2827.9
3746.4
5010.0
25806.5
7142.2
• Page 28 <
AGUA HED10N&A LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY2003
AH-0425-2Q
AH-042943
10/17/01 ua Hedionda. WQ Station #WS1
qua Hedionda Outer NWBuoy
gja Hedionda Outer NWBuoy
gua Hedionda Outer NWBuoy 180
AH-0429-O4 11/05/01 0945 ,gua Hedionda Outer NWBuoy WBMU 180
AH-0429-05 11/05/01 1000 Aqua Hedionda. WQ Station #05N CBMU 180 485.2
AH-0429-06 11/05/01 1000 Agua Hedionda. WQ Station #05N CBMU 180
AH-0429-07 11/05/01 1000 Agua Hedionda, WQ Station #05N CBMU 9500
AH-0429-08 11/05/01 1000 Agua Hedionda. WQ Station #OSN CBMU 180
AH-0429-09 11/05/01 1015 Agua Hedionda. WQ Station #05 CBMU 2300 3320.0
AH-0429-10 11/05/01 1015 Agua Hedionda, WQ Station #05 CBMU 2200
AH-0429-11 11/05/01 1015 Agua Hedionda. WQ Station #05 CBMU 4900
AH-0429-12 11/05/01 1015 Agua Hedionda, WQ Station #05 CBMU 4900
AH-0429-13 11/05/01 1030 Agua Hedionda, WQ Station #05S CBMU 450 1229.1
AH-0429-14 11/05/01 1030 Agua Hedionda, WQ Station #05S CBMU 450
AH-0429-15 11/05/01 1030 Agua Hedionda, WQ Station #05S CBMU 4900
AH-0429-16 11/05/01 1030 Agua Hedionda, WQ Station #05S CBMU 2300
AH-0429-17 11/05/01 1040 Agua Hedionda, WQ Station #WN1 CBMU 370 271.0
AH-0429-18 11/05/01 1040 Agua Hedionda, WQ Station #WN1 CBMU 180
AH-0429-19 11/05/01 1040 Agua Hedionda. WQ Station #WN1 CBMU 180
AH-0429-20 11/05/01 1040 Agua Hedionda. WQ Station #WN1 CBMU 450
AH-0429-21 11/05/01 1050 Agua Hedionda. WQ Station #WS1 CSMU 450 284.6
AH-0429-22 11/05/01 1050 Agua Hedionda. WQ Station #WS1 CBMU 450
AH-0429-23 11/05/01 1050 ft
IA.
.gua Hedionda. WQ Station #WS1 CBMU 180
AH-0429-24
AH-0430-01
11/05/01 1050 Agua Hedionda, WQ Station #WS1
11/06/01 0945
CBMU
Agua Hedionda Outer NW Buoy WBMU
180
180 180.0
AH-0430-02 11/06/01 0945 Agua Hadionda Outer NW Buoy WBMU 180
AH-0430-03 11/06/01 0945 Agua Hedionda Outer NW Buoy WBMU 180
AH-0430-04 11/06/01 0945 Ag ja Hedionda Outer NW Buoy WBMU <180
7 WBMU = Wild Bay Mussel (i.e., natural set)
• Page 29 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
AH-0430-05
AH-0430-06
AH-0430^07
AH-0430-08
AH-0430-09
AH-043Q-10
AH-0430-11
AH-O430-12
AH-0430-13
AH-0430-14
AH-0430-15
AH-0430-16
AH-0430-17
AH-0430-18
AH-0430-19
AH-0430-20
AH-0430-21
AH-0430-22
AH-0430-23
AH-0430-24
AH-0431-01
AH-0431-02
AH-0431-03
AH-0431-04
AH-0431-05
AH-0431-06
AH-0431-07
AH-0431-OS
AH-0431-09
AH-0431-10
AH-0431-11
AH-0431-12
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
1 1/06/01
1 1/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/07/01
11/07/01
1 1/07/01
1 1/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
1000
1000
1000
1000
1037
1037
1037
1037
1110
1110
1110
1110
1145
1145
1145
1145
1200
1200
1200
1200
0900
0900
0900
0900
1000
1000
1000
1000
0925
0925
0925
0925
Agua Hedionda, WQ Station #05N
Aqua Hedionda. WQ Station #05N
Agua Hedionda, WQ Station #05N
Aaua Hedionda. WQ Station #05N
Agua Hedionda. WO Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda. WQ Station #053
Agua Hedionda. WQ Station #Q5S
Agua Hedionda. WQ Station #05S
Agua Hedionda, WQ Station #WN1
Agua Hedionda. WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda. WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda Outer NW Buoy
Agua Hedionda Outer NW Buoy
Agua Hedionda Outer NW Buoy
Agua Hedionda Outer NWBuoy
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #Q5N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
CBMU
CBML)
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
C8MU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
WBMU
WBMU
WBMU
WBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
>
>
>
<
<
<
<
<
<
<
<
<
160000
160000
160000
160000
130000
2300
130000
4900
130
4900
4900
2300
180
4900
180
180
1300
450
930
2300
180
180
180J
450
4900
2300
24000
4900
2300
2300
180
130
160000.0
20890.7
1775.6'
411.2
1057.6
226.3
6033.7
643,4
• Page 30 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
AH-C431-13
AH-0431-14
AH-0431-15
AH-0431-1G
AH-C431-17
AH-0431-18
AH-0431-19
AH-0431-20
AH-0431-21
AH-0431-22
AH-0431-23
AH-Q431-24
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
1040
1040
1040
1040
0945
0945
0945
0945
1100
1100
1100
1100
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #058
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #WN1
Aqua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
CBMU
<
<
<
<
<
<
<
24000
4900
24000
4900
180
180
180
180
2300
180
180
180
10844.4
180.0
340.3
• Page 31 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Table 10. Geometric means for shellfish fecal colifbrm results for each site per sampling event.
1302
1233
3547
1183
'3994
7766
1637
5910
2871
972
3131
272
706
'2795
'468 194
1 Page 321
co
a
s
en
cn
QJ
o
ow
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Table 12. Results of the Wilcoxson Rank Sums Test and the Kruskal-Wallis Test for fecal
coliform concentration data from mussel samples.
111.1
#05 36 3673 3474 297.7 102.0
#053 36 4351 3474 297.7 120.9
#WN1 36 3026 3474 297.7 84.0
#WS1 36 3161 3474 297.7 87.8
Buoy 12 314 1158 184.6 26,2
Chi Square 32.26
DF
Probability > Chi Square < 0.0001
Page 34 <
AQUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 13. Results of the Kruskal-Wallis test for differences among sites in the bird-
occupied area.
z
One-Sided Probability < Z
TwoOSided Probability > Z
-4.2548
< 0.0001
< 0.0001
• Page 35 •
AGUA H&3K3NOA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 14. Summary of the ranks of shellfish fecal coliform concentrations for each event ana* sampling station.
EVENTS:
- w/o Buoy E2 E1 E3 Yes .065
-with Buoy E2 E1 E3 Yes .004
srres
- w/o Buoy 5S 5N WS1 WN1 Yes .017
- with Buoy 5S 5N WS1 WN1 Yes .000
• Page 36 •
AGISAHEOIQHDA LAGOON SHELLFiSH TECHNICAL AOWSQRY COMMITTEE REPQRr JANUARY 2003
Table 15. Fecal coliform results for water samples from all sampling events.
Eventl, Day1:
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02KJ1
12:15 Ugua Hedionda, WQ Station #04
12:15
12:20
12:20
12:26
12:26
12:33
12:33
12:38
12:38
12:41
12:41
12:44
12:44
12:44
12:44
12:49
12:49
12:49
12:49
12:54
12:54
12:54
12:54
13:00
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Aqua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #07
Aqua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #C5N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #WN1
SGW9 II 110.01 93.2
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
^SGW
SGW
79.0
7.8
33.0
22.0
23.0
49.0
11.0
4.5
7.8
7.8
11.0
33.0
23.0
17.0
17.0
6.8
4.5
9.3;
6.8
33.0
79.0
46.0
79.C
13.C
16.0
22.5
23.2
5.9
9.3
21.6
6.6
55.5
15.8
' SGW = Shellfish Growng Water
• Page 37 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
26
27
28
29
30
31
32
Event 1, Day 2:
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
13
19
20
21
22
23
24
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/02/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03J01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
13:00
13:00
13:00
13:04
13:04
13:04
13:04
11:30
11:31
11:37
11:38
11:44
11:45
11:50
11:52
11:54
11:55
11:56
11:56
12:04
12:05
12:06
12:06
12.08
12:09
12:10
12:11
12:12
12:14
12:15
12:15
Agua Hedionda, WQ Station #WN1
Agua Hedionda. WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda. WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #06
Agua Hedionda. WQ Station #07
Agua Hedionda. WQ Station #07
Agua Hedionda. WQ Station #40
Agua Hedionda. WQ Station #40
Agua Hedioncfa. WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05 N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Aqua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #058
Agua Hedionda, WQ Station #058
Agua Hedionda, WQ Station #058
Aaua Hedionda, WQ Station #05S
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
4.5
49.0
22.0
17.0
13,0
13.0
17.0
49.0
49.0
14.0
49.0
4.Q
33.0
17.0
17.0
33.0
33.0
49.0
49.0
23.0
49.0
33.0
7.8
6.8
79.0
7,8
4.5
79.0
70.0
79.0
33.0
14.9
49.0
26.2
11.5
17.0
33.0
49.0
23.2
11.7
61,6
* Page 38 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITT££ REPORT JANUARY 2003
25
26
27
28
29
30
31
32
Event 1, Day 3:
01
•02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/03/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
12:20
12:21
12:21
12:24
12:26
12.27
12:29
12:29
11:15
11:15
11:20
11:20
11:23
11:23
11:27
11:27
11:29
11:29
11:31
11:31
11:33
11:33
11:33
11:33
11:38
11:38
11:38
11:38
11:45
11:45
11:45
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda. WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda. WQ Station #WS1
Agua HedFonda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Agua Hedionda. WQ Station #06
Agua Hedionda. WQ Station #07
Apua Hedionda, WQ Station #07
Agua Hedionda. WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua HedFonda. WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
33.0
31.0
33.0
70.0
17.0
34.0
49.0
49.0
33.0
14.0
23.0
79.0
<1.8
13.0
4.5
4.5
13.0
7.8
7.8
4.0
17.0
7.8
4,5
13,0
33.0
13,0
17.0
2,0
260.0
23.0
33.0
39.2
34.3
21.5
42.6
13.0
4.5
10.1
5.6
9.4
11.0
71.2
Page 39 <
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL AD VISOFtY COMMITTEE REPORT JANUARY 2003
24
25
26
27
28
29
30
31
32
••. : ••.:-::- :- • • : •-• .
Event 2, Day 1: :
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/04/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
11:45
11:52
11:52
11:52
11:52
11:55
11:55
11:55
11:55
13:50
13:50
13:55
13:55
14:00
14:00
14:10
14:10
14:15
14:15
14:15
14:15
14:20
14:20
14:20
14:20
14:22
14:22
14:22
14:22
14:25
Agua Kedionda, WQ Station #05S
Aqua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
SOW
SOW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
130.0
<1.8
11-0
4.5
49.0
17.0
11.0
11.0
NR*
13.0
11.0
4.0
6.8
4.5
17.0
11.0
17.0
23.0
22.0
13.0
23.0
23.0
7.8
33,0
70.0
14.0
11.0
22.0
23.0
7.8
13.4
12.7
12.0
5.2
8.7
13.7
22,5
17,3
25.4
16.7
15.0
Page 40 i
AGUA HSD1ONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
22
23
24
25
26
27
28
29
30
31
32
Eveht2,bay;2:;; ! r
01
02
03
04
05
OS
07
08
09
10
11
12
13
14
15
16
17
18
19
20
10/W01
10/15/01
10/1 5/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/15/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
14:25
14:25
14:25
14:30
14:30
14:30
14:30
14.35
14:35
14:35
14:35
11:52
11:52
12:00
12:00
12:07
12:07
12:13
12:13
12:15
12.15
12:16
12:16
12:19
12:19
12:19
12:19
12:23
12:23
12:23
12:23
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Aqua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Aqua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #QSN
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
<
<
17.0
49.0
7.8
13.0
13.0
11.0
11.0
17.0
17.0
17.0
11.0
130.00
1.80
49.00
17.00
3.70
33.00
23.00
33.00
17.00
7.BQ
6.80
13.00
17.00
4.00
7.80
7.80
6.80
11.00
7.80
7.80
12.0
15.2
15.3
23.9
11.0
27.5
11.5
9.4
8.0
8.2
• Page 41 *
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
21
22
23
24
25
26
27
28
29
30
31
32
Event 2. Day 3: i
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/16/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
10/17/01
12:28
12:28
12:28
12:28
12:34
12:34
12:34
12:34
12:36
12:36
12:36
12:36
11:06
11:06
11:15
11:15
11:20
11:20
11:23
11:23
11:25
11:25
11:29
11:29
11:30
11:30
11:30
11:30
11:35
11.35
11:35
Agua Hedionda. WQ Station #05S
Aqua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #058
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Asua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #03
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
33.00
7.80
13.00
1.80
13.00
7.80
7.80
4.5(
11.00
7.80
13.00
6.80
79.d
70.0
79.0
17.0
4.5
<1.8
7.8
7.8
4.5
2.0
4.5
23.0
7.8
11.01
7.8
13.0
22.0
7.6
11.0
8.8
7.7
9.3
74.4
36.6
4.5
7.8
3.0
10.2
9.7
17.4
» Page 42 *
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
20
21
22
23
2*
25
26
27
28
29
30
31
32
10/17/01 11:35 Aqua Hedionda,. WQ Station #05 SOW 49.0
10/17/01 11:40 Aqua Hedionda. WQ Station #05S SOW 17.0 16.4
10/17/01 11:40 Aqua Hedionda. WQ Station 305S SOW 23.C
10/17/01 11:40 Agua Hedionda. WQ Station #055 SGW 11.0
10/17/01 11:40 Agua Hedionda. WQ Station #05S SGW 17.0
10/17/01 11:47 Aaua Hedionda. WQ Station #WN1 SGW 2.0 2.a
10/17/01 11:47 Agua Hedionda. WQ Station #WH1 SGW 2.0
10/17/01 11:47 Agua Hedionda, WQ Station #WN1 SGW 2.0
10/17/01 11:47 Agua Hediortda. WQ Station #WN1 SGW 7.8
10/17/01 11:50 Agua HediorvJa. WQ Station #WS1 SGW 11.0 6.2
10/17/01 11:50 Agja Hediortda, WQ Station #WS1 SGW 2.0
OJ_
02_
03_
04_
05_
06_
07_
°JL
09
Station #WS1 SGWqja Hedionda, WQ
ua Hedionda, WQ Station #WS1
qua Hedionda WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
11/05/01 11:40 Agua Hedionda, WQ Station #06 SGW 11.0
11/05f01 11:50 Agua Hedionda, WQ Station #07 SGW 1.8 1.8
11/05/01 11:50 Agua Hedionda, WQ Station #07 SGW 1.8
11/05/01 11:55 Agua Hedionda Outer NW Buoy SGW 4.5
11/05/01 11:55 Agua Hedionda Outer NW Buoy SGW 2.0
11/05/01 11:55 Agua Hedionda Outer NW Buoy SGW 1.8
11/05/01 11:55 ftgua Hedionda Outer NW Buoy SGW 1.8
11/05/01 12:00 Agua Hedionda, WQ Station #40 SGW 4.5 3.0
11/05/01 12:00 Agua Hedionda, WQ Station #40 SGW 2.0
11/05/01 12:01 Agua Hedionda, WQ Station #41 SGW 1.8 1.9
TJ
~2~3
11/05/01 12:01 Agua Hedionda, WQ Station #41 SGW 2.0
15
16_
17
11/05/01 12:03 Agua Hedionda. WQ Station #42 SGW 1.8
11/05/01 12:03 Agua Hedionda, WQ Station #42 SGW 1.8
11/05/01 12:05 lAgua Hedionda, WQ Station #05N SGW |<
« Page 43 •
AGUA HBDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
18
19
20
21
22
23
24
25
26
27
26
29
30
31
32
33
34
35
02
03
04
05
06
07
08
09
10
11
12
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
11/05/01
1V06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
12:05
12:05
12:05
12:08
12:08
12:08
12:08
12:15
12:15
12:15
12:15
12:20
12:20
12:20
12:20
12:30
12:30
12:30
12:30
12:49
12:49
12:58
12:58
13:05
13:05
13.10
13:10
13:10
13:10
13:15
13:15
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Ague Hedionda, WQ Station #05
4,5 ua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Ague Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Ao.ua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hed Fonda, WQ Station #06
Agua Hedionda, WQ Station #06
Agua Hed Fonda, WQ Station #07
Agua Hedionda, WQ Station #07
Agua Hedionda Outer NW B joy
Agua Hedionda Outer NW Buoy
Agua Hedionda Outer NWBuoy
Agua Hedionda Outer NWBuoy
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
SGW
SOW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
<
<
<
<
<
<
<
<
<
<
4.5
2.0
1.8
1.8
1.8
1.8
4.0
7.8
7.8
11.0
11.0
1.8
2.0
1.8
*
1.8
4.5
1.8
2.0]
33.0
33.0
4.1
16.0
2.0
<1.8
13.0
<1.8
4.0
2.0
4.5
4.5
2.2
9.3
1.9
.2.3
33.6
8.1
2.0
4.7
4.5
• Page 44 •
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
26
29
30
31
32
33
34
35
01
02
03
04
05
06
07
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/06/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
13:18
13:18
13:21
13:21
13:24
13:24
13:24
13:24
13:30
13:30
13:30
13:30
13:36
13:36
13:36
13:36
13:45
13:45
13:45
13:45
13:50
13:50
13:50
13:50
11:50
11:50
11:56
11:58
12:00
12:00
12:08
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda. WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #053
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #04
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #06
Agua Hedionda, WQ Station #07
Agua Hedionda, WQ Station #07
Agua Hedionda Outer NWBuoy
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
<
<
<
<
<
<
1.8
7.8
1.8
1.8
4.5
2.0
3.8*
4.5
4.5
4.S
7.8
1.8
13.0
49.0
23.0
23.0]
17.0
4.5
23.0
6.8
7.8
7.8
17.0
11.0
17.0
70.0
13.C
2.0
1.8
1.8
3.2*
3.7
i.e!
3.4
4.1
24.1
10.5
8.7
34.5
5.1
1.8
1.9
Page 45 <
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2QQ3
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
26
29
30
31
32
33
34
35
36
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
11/07/01
12:08
12:08
12:08
12:15
12:15
12:16
12:16
12:17
12:17
12:20
12:20
12:20
12:20
12:26
12:26
12:26
12:26
12:30
12:30
12:30
12:30
12:35
12:35
12:35
12:35
12:37
12:37
12:37
12:37
Agua Hedionda Outer NW Buoy
Agua Hedionda Outer NWBuoy
Agua Hedionda Outer NWBuoy
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #40
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #41
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #42
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05N
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda, WQ Station #05S
Agua Hedionda h WQ Station #05S
Agua Hedionda, WQ Station #WNM
Agua Hedionda, WQ Station #WNI1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station #WN1
Agua Hedionda, WQ Station SWS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
Agua Hedionda, WQ Station #WS1
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
SGW
<
<
<
1.8
1.8
2.0
6.8
2.0
4.5
2.Q
4.5
2.0
4.0
2.0
4.5
2.0
2.0
4.5
2.0
4.5,
17.0
7.8
23.0
33.0
1.8
1.3
2.0
1.8
4.5
2.0
2.0
6.8
3.7
3.0
3.0
2.9
3.0
17.8
1.3
3.3
• Page 46 •
AGtW HECHONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 16. Geometric means for water fecal coliform results for each site per sampling event.
Page 47 *
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTSS REPORT: JANUARY 2003
Table 17a. Results of the Kruskal-Wallis Non-Parametric Rank Test for fecal coliform
concentrations in water samples from the bird-occupied and bird-free areas.
Bird-Occupied:
05N 36 82
05 36 72
053 36 129
Bird-Free:
WN1 35 77
WS1 35 87
TOTAL 178
Chi-Square 27.702
Degrees of Freedom
Asymmetrical Significance 0.000
Table 17b. Results of the Kruskal-Wallis Non-Parametric Rank Test for fecal colHbrm
concentrations in water samples from the bird-occupied and bird-free areas, with site
#05S removed.
Bird-Occupied:
05N
05
Bird-Free:
WN1
WS1
TOTAL
36
36
35
35
142
73
66
69
78
Chi-Square
Degrees of Freedom
Asymmetrical Significance
1.9
3
0.593
i Page 48 •
AGUA HED1ONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 18. Results of the Mann-Whitney Test of fecal coliform concentrations in water
samples from the bird-occupied and bird-free areas.
AREA
Bird-occupied
Bird-free
Total
Mann-Whitney U
Wilcoxon W
Z
Asymmetric
Significance
N
108
70
178
MEAN RANK
94.34
82.03
SUM OF RANKS
10189
5742
3257
5742
-1.563
.118
• Page 49 »
AGUA HEDIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
Table 19. Comparison of inter laboratory results for fecal colrform concentrations in shellfish samples.
10CafcuEated from replicate values
11 From: U.S. Food and Drug Administration Bacteriotogical Analytical Manual, 8* Edition {Revision A), 1998,
Geometric mean of replicate values
• Page SO •
AGUA HEDIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
E1D302QC
E1D303QC
E2D101QC
E2D102QC
E2D103QC
E2D201QC
E2D202QC
E2D203QC
E2D301QC
10/04/01
10/04/01
10/15/2001
10/1 5/2001
10/15/2001
10/16/01
10/16/01
10/16/01
10/17/01
tf05
(WVN1
&05N
005
W/VN1
tfQSN
SOS
wsi
#05
1100
700
68
20
<18
3300C
110C
130C
170C
2400
2400
2400
1300
>16000
5400
2400
2400
2400
180
450
<180
<180
<180
<180
<180
<180
<180
<180
<180
<180
>160000
>160000
24000
>160000
4900
2300
2300
2300
22000
13000
4900
950C
230C
2300
3308
2939
226
<180
<=180
99573
2779
10742
3746
135C
2106
15£
<18C
<18C
4563S
2036
6411
2304
8107
4103
330
<180
<180
217243,
3792
17998
6092
1000
1000
70
34000
1000
3400
1000
10000
7100
400
230000
7100
250000
7100
Page 51
AGUA HED10NDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
E2D302QC
E2D3Q3QC
E3D101QC
E3D102QC
E3D103QC
E3D201QC
E3D202QC
E3D203QC
E3D3Q1QC
10/17/01
10/17/01
11/05/01
11/05/01
11/05/01
11/06/01
11/06/01
11/06/01
11/07/01
#05S
#WS1
Juoy
WJ5
#WN1
#G5N
#053
#WS1
#05N
220
430
<18
680C
68
<18
24000
68
70C
4900
7600
490C
13000
230C
4300
4300
9500
13000
4900
<180
<18Q
<180
<180
2300
2200
4900
4900
370
<180
<180
450
> 160000
>160000
>16000C
16000C
18C
490C
490C
230C
130{
45(
930
2300
490C
5010
7142
<180
3320
271
16000C
1776
1058
6034
278C
4623
<18C
2293
183
160000
489
604
267S
9030
11035
<180
4806
402
160000
6443
1852
13588
1500
2200
1000
100
40000
70C
34C
2200
17000
1700C
7100
710
460000
4000
2500
15000
• Page 52 •
AGUA HEDIONDA. LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2003
E3D302QC
E3D303QC
11/07/01
1 1/07/01
#05S
#WS1
1700
18.C
2300
24000
4900
24000
4900
24000
4900
230C
<18t
<18C
<18C
10844
34C
5100
11S
23059
970
360C
10C
25000
1000
Page 53
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Table 20. Comparison of inter laboratory results for fecal coliform concentrations in water samples.
Calculated from replicate values
14 From: U.S. Food and Drug Administration Bacteriological Analytical Manual, 8th Edition (Revision A), 1998.15 Geometric mean of replicate values
• Page 54 *
AGUA HEDIONDA WGOOW SHELLFiSH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
AH-Q42G-02
ftH-0426-03
AH-M27-01
W-Q427-02
WH-0427-03
AH-0428-01
AH-0428-02
WH-0428-03
10/15/01
10/15/01
10/16/01
10/16/01
10/16/01
10/17/01
10/17/01
10/17/01
JTO7
ros
MO
*42
JW5H
ff06
(*41
S05N
99
43
4£
4E
4E
4£
32
2fl
c
17
14
11
22
23
23
33
7
13
17
4
8
8
79
17
5
2
8
11
8
13
9
17
28
9
8
37
3
10
;
12
20
6
c
10
2
a
2S
22
37
16
13
130
6
12
3.4
6.C
s.e
3.4
3.4
14.C
0.7
3.5
22
3£
7C
22
22
10C
10
22
• Page 55 •
AGUA HEQIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
FIGURES 1-4.
• Page 56 *
A&JA HEDIQNDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Figure 1. Location of sampling stations.
• Page 57 •
AGUA HEDSONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT. JANUARY 2003
Figure 2. Locations of mussel lines and numbering system used for counting birds.
NORTH
AQUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT: JANUARY 2003
Figure 3. Geometric means of the fecal coliform data for shellfish samples by sampling
site (5N, 5, and 53 are in the bird-occupied area, WN1 and WS1 are in the bird-free
area).
Geometric Means for Each Event: Shellfish
< Page 59 >
AGUA HEDIONDA LAGOON SHELLFISH TECHNICAL ADVISORY COMMITTEE REPORT JANUARY 2Q03
Figure 4. Geometric means of the fecal coliform data for water samples by sampling site
(5N, 5, and 5S are in the bird-occupied area, WN1 and WS1 are in the bird-free area).
Geometric Means for Each Event: Waters
i Page 60 <