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; Carlsbad Municipal Golf Course Toxicity Testing; Toxicity Testing for Municipal Golf Course Water Quality; 2006-07-05
Results of Toxicity Testing for The City of Carlsbad - Municipal Golf Course Water Quality Study Prepared for: Dudek & Associates, Inc. 605 Tliird Street Encinitas, CA 92024 Testing Location: Nautilus Environmental 5550 Morehouse Drive, Ste. 150 San Diego, CA 92121 Submitted: July 5, 2006 Data Qualitv Assurance: • Nautilus Environmental is a state certified laboratory under the California Department of Health Services - Environmental Laboratory Accreditation Program (ELAP), Certificate No. 1802. • All test results included in this report have met internal Quality Assurance requirements, as well as all minimum acceptability criteria for test controls under the EPA protocol requirements. • All data have been reviewed and verified. Results Verified By Date: ^-^-oC TOXICITY SUMMARY REPORT Test ID Number: 0605-S065 a, b, c, and d Client: Dudek & Associates Prepared: June 28, 2006 Sample Information Client: Project Study: Sample Site ID's: Sample Collection Date, Time: Sample Receipt Date, Time: Sample Materiai: Dudek & Associates, Inc. Carlsbad Golf Course Toxicity Evaluation a) Upstream Surface b) Downstream Surface c) Well #1 d) Well #3 6/1/06, 1245 to 1500 (for a, b, c); and 6/2/06, 0930 (for d) 6/2/06, 1200 Grab samples; groundwater and site runoff Chronic Toxicity Test Specifications Test Period: Test Organism: Test Endpoints: Test Organism Source, Age: Test Concentrations: Lab Control/Dilution Water: Conductivity Controls: Protocol Used: Test Acceptability Criteria: Test Validity: 6/2/06 to 6/9/06 Ceriodaphnia dubia (water flea) 7-day chronic survival and reproduction In-house culture, <24 hours 100, 50, 25, 12.5, and 6.25%; plus Lab and Conductivity Controls Lab freshwater (diluted mineral water); conductivity = 200 pS/cm Lab freshwater spiked with seawater to raise the conductivity to levels matching the samples (3,600 pS/cm and 4,800 pS/cm), These controls were conducted to evaluate the potential effects due to conductivity alone. EPA/821/R-02/013, 2002 Freshwater Chronic The Lab Control must meet or exceed a mean of 80% survival; 60% of the surviving females must produce 3 or more broods of offspring; and surviving females must produce a mean of 15 or more offspring (only the first 3 broods are used for calculating mean reproduction). Test results met all test acceptability criteria and internal QA/QC requirements. Table 1. Water Quality Information: Samples Upon Receipt Sample ID pH DO (mg/L) Temp CC) Conductivity (nS/cm) Hardness (mg/L as CaCOs) Alkalinity (mg/L as CaCOj) Upstream 7.49 8,4 9.1 3,910 >1,000 202 Downstream 7.79 8,1 13.5 3,590 >1,000 245 Well #1 6.32 4,2 " 8.1 3,720 968 71 Well #3 6,63 2.7" 8.3 4,820 >1,000 250 Samples were aerated prior to testing, to raise dissolved oxygen levels up to around 8,0 mg/L. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Test ID Number: 0605-S065 a, b, c, and d Client: Dudek & Associates Prepared:June 28, 2006 Table 2. Summary of Test Results Sample ID Concentration (%) Mean Percent Survival Mean Number of Offspring Produced Lab Control 100 32 Upstream 6,25 100 31 12,5 100 30 25 100 32 50 90 26 100 90 25 * Lab Control 100 32 Downstream 6,25 89 29 12,5 100 34 25 100 31 50 100 28 100 90 ' 25 * Lab Control • 100 33 Well #1 6,25 100 34 12.5 100 32 25 100 34 50 100 31 100 90 14 * Lab Control 100 33 Well #3 6,25 100 31 12.5 100 31 25 100 33 50 90 27 * 100 90 8* Conductivity Control #1 3,600 pS/cm 100 20 * Conductivity Control #2 4,800 pS/cm 40 5 * * an asterisk indicates a statistical difference (i.e. toxicity) when compared to the Lab Control Note: Conductivity Control #1 had a conductivity matching the Upstream, Downstream and Well #1 samples, while Conductivity Control #2 had a conductivity matching the Well #3 sample. Nautilus Environmental - San Diego Bioassay Laboratory TOXICITY SUMMARY REPORT Test ID Number: 0605-S065 a, b, c, and d Client: Dudek & Associates Prepared: June 28, 2006 Table 3. Summary of Statistical Results and Reportable Values Sample ID Test & Endpoint NOEC (% effluent) ECso (% effluent) TUc (toxicity units) Upstream: Survival Reproduction Downstream: Survival Reproduction Well#1: Survival Reproduction 100 50 100 50 100 50 >100 >100 >100 >100 >100 93.4 1.0 2.0 1.0 2.0 1.0 2.0 Well #3: Survival Reproduction 100 25 >100 77.7 1,0 4,0 NOEC - the highest Concentration that produces No Observed Effect ECso = concentration that causes an adverse effect to 50% of the test organisms Chronic Toxicity Units (TUc) = 100 T NOEC Discussion of Results All testing met the EPA protocol's minimum test acceptability criteria, as well as all internal requirements under our Quality Assurance Program. Survival of Ceriodaphnia was not affected in any of the four samples tested. However, all four samples did result in some degree of toxicity with regards to the reproduction endpoint. Adverse effects to the reproduction were expected, however, due to the elevated conductivity of the samples. Through internal studies and experience, we have consistently observed reproductive effects to Ceriodaphnia when conductivity levels exceed approximately 2,500 pS/cm, Conductivity is related to the concentration of salts in a sample. The degree of effect on reproduction of C. dubia was related to conductivity during this monitoring study as well. Well # 3, with the greatest conductivity (4,800 pS/cm), had the greatest effect on the number of offspring produced (a 76% reduction from the control). To evaluate potential effects due to conductivity alone, laboratory controls of the same conductivity as the samples were created and tested concurrently. The conductivity control results closely matched that of their respective samples and were not statistically different, further suggesting that conductivity could account for the reproductive effects observed in all samples tested. Nautilus Environmental - San Diego Bioassay Laboratory Appendix A Chronic Toxicity Testing Raw Data and Statistical Analysis Site: Upstream CETIS Test Summary Report Date: Page 1 of 2 27 Jun-06 11:58 AM Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Test No: 14-2485-4300 Test Type: Reproduction-Survival (7d) Duration: 6d 23ti Start Date: 02 Jun-06 01:50 PM Protocol: EPA/821/R-02-013 (2002) Species: Ceriodaphnia dubia Ending Date: 09 Jun-06 01:45 PM Dil Water: Diluted Mineral Water (8:2) Source: In-House Culture Setup Date: 02 Jun-06 01:50 PM Brine: Not Applicable Sample No: 02-5879-7542 Material: Upstream Ambient Sample Client: Dudek & Associates Sample Date: 01 Jun-06 12:45 PM Code: 06-0463 Project: Receive Date 02 Jun-06 12:00 PM Source: Dudek & Associates Project: Sample Age: 25ti . station: Upstream Comparison Summary Analysis Endpoint NOEL LOEL ChV MSDp Method 00-5972-8276 7d Proportion Survived 100 > 100 N/A N/A Fisher's Exact 03-4480-2055 Reproduction 50 100 70.711 14,72% Steel's Many-One Rank Test Acceptability Analysis Endpoint Attribute Statistic Acceptable Range Decision 00-5972-8276 7d Proportion Survived Control Response 1 0.8 - N/A Passes acceptability criteria 03-4480-2055 Reproduction Control Response 31.5 15- N/A Passes acceptability criteria 03-4480-2055 Reproduction MSDp 0.14721 0,13-0.47 Passes acceptability criteria 7d Proportion Survived Summary Conc-% Control Type Reps Mean IVIinimum Maxirnum SE SD CV 0 Conductivity C 10 1.00000 1.00000 1.00000 0.00000 0,00000 0.00% 0 Lab Control 10 1.00000 1.00000 1,00000 0.00000 0,00000 0,00% 6.25 10 1.00000 1.00000 1,00000 0.00000 0,00000 0.00% 12.5 10 1.ooooo 1.00000 1,00000 0,00000 0,00000 0.00% 25 10 1.00000 1.00000 1,00000 0,00000 0,00000 0,00% 50 10 0,90000 0.00000 1,00000 0,10000 0,31623 35.14% 100 10 0,90000 0.00000 1,00000 0,10000 0:31623 35.14% Reproduction Summary Conc-% Control Type Reps Mean IVIinimum IVlaximum SE SD CV 0 Conductivity C 10 20 8 27 2.087 6,5997 33.00% 0 Lab Control 10 31.5 26 35 0,8199 2.5927 8.23% 6.25 10 31.3 27 36 0,8699 2.7508 8:79% 12.5 10 29.9 25 34 1,0899 3.4464 11.53% 25 10 32,1 27 37 0,9481 2,9981 9.34% 50 10 26,2 6 35 2,5768 8,1486 31.10% 100 10 24,6 14 31 1.4772 4,6714 18.99% 000-089-125-1 CETIS™ V1.025B Analyst:_ Approval: C//^ 7/'^0(^ CETIS Test Summary Page 2 of 2 Report Date: 27 Jun-06 11:58 AM Link: 03-4627-6794/0605-S065a 7d Proportion Survived Detail Conc-% Control Type Rep 1 0 0 6.25 12.5 25 50 100 Rep 2 Rep 3 Conductivity C 1.00000 1.00000 1.00000 LabControl 1,00000 1,00000 1,00000 1,00000 1,00000 1.00000 1,00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1,00000 1.00000 Rep 4 1,00000 1.00000 1,00000 1,00000 1,00000 1,00000 1,00000 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 1,00000 1,00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 10 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 1.00000 Reproduction Detail Conc-% Contro 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0 0 6.25 12.5 25 50 100 Conductivity C Lab Control Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 15 11 8 24 27 25 26 20 20 24 32 32 34 35 31 26 30 30 34 31 36 33 30 32 30 31 27 30 35 29 34 25 33 33 25 33 31 30 27 28 34 30 35 37 31 34 • 29 32 32 27 32 32 32 35 24 26 26 6 25 24 23 31 28 26 21 28 25 26 • 14 24 000-089-125-1 CETIS™ V1.025B Analyst:. Approval: V/^ TAyt?^ CETIS Analysis Detail Comparisons: Report Date: Page 1 of 1 27 Jun-06 11:58 AM 00-5972-8276/0605-S065a Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version 7d Proportion Survived Comparison 03-4627-6794 03-4627-6794 27 Jun-06 11:57 AM CETlSvl .025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Fisher's Exact C > T Untransformed 100 >100 1.00 N/A . Group Comparisons Control vs Conc -% Statistic Critical Decision(0.05) Lab Control 6,25 12.5 25 50 100 1,00000 1.00000 1.00000 0.50000 0,50000 0.05000 0.05000 0.05000 0.05000 0.05000 Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed 0 Lab Control 10 0 10 6.25 10 0 10 12.5 10 0 10 25 10 0 10 50 9 1 10 100 9 1 10 Graphics 1.0-o o o o 0.9- •P o o V .> 0.8- « 0.7- .1 0.. 1 o.s- T) 0.1- 0,3: 0,2: 0,1- 0,0 0,0 0 6,25 1 12.5 25 Conc-% r 1 i 50 100 000-089-125-1 CETIS™ v1,025B Analyst:. Approval: ^fl '7/^/o€ CETIS Analysis Detail Comparisons: Report Date: Analysis: Page 1 of 1 27 Jun-06 11:58 AM 03-4480-2055/0605-S065a Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 03-4627-6794 03-4627-6794 27 Jun-06 11:57 AM CETlSvl.025 Method Alt H Data Transform Steel's Many-One Rank C>T Untransformed NOEL LOEL Toxic Units ChV 50 100 MSDp 2.00 70.711 14,72% ANOVA Assumptions Attribute Test statistic Critical P Level Decision(O.OI) Variances Bartlett 19.51903 15.08628 0.00154 Unequal Vanances Distribution Kolmogorov-Smirnov D 0.13133 0.13307 0,01175 Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F statistic P Level Decision(0.05) Between 487.3333 97.46667 5 4.74 0,00116 Significant Effect Error 1110.4 20.56296 54 Total 1597.73337 118.02963 59 Group Comparisons Control vs Conc-% Statistic Critical P Level Ties Decision(0.05) Lab Control 6.25 99.5 75 > 0,0500 5 Non-Significant Effect 12.5 92 75 > 0,0500 6 Non-Significant Effect 25 110.5 75 > 0,0500 5 Non-Significant Effect 50 83.5 75 > 0,0500 7 Non-Significant Effect 100 62 75 <= 0.0500 6 Significant Effect Data Summary Original Data Transformed Data Conc-% Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD 0 Lab Confrol 10 31.5 • 26 35 2,5927 6.25 10 31.3 27 36 2,7508 12.5 10 29.9 25 34 3,4464 25 10 32.1 27 37 2,9981 50 10 26.2 6 35 8,1485 100 10 24.6 14 31 4,6714 Graphics S 1 I 30-1 I 1 I 1 I j 12,5 25 Conc-% Rankits 000-089-125-1 CETIS™ v1,025B Analvst: Approval: '^^ lU/06 Freshwater Chronic Bioassay Client/Sample ID: Test No: Dudek / Upstream Surface biros'- $00-5' Conc. Rep Board/ Cup Daily Reproduction/ Survival Total QC Conc. Rep Board/ Cup !;!:ii1;;;n m:5:m. m^mi mTim Total QC Lab Control 1 HC 1 0 o iir c Lab Control 2 KS ?. 0 o 0 32. m-m^$. 3 15 H 0 O s , ^ 0 m-m^$. 4 1 0 o T m Q m-m^$. 5 HS s 0 o 0 l-r ll 0 7( m-m^$. 6 H5 f> o 0 , :^ o '•^ 0 / ; m-m^$. 7 HS T 0 r ^'^^ -o -(^ ^l 30 —"-^—f- m-m^$. 8 H5 fi o ^ '> 0 ' 1^ o M 0 m-m^$. 9 HS 10 0 o o IV ? VCP m-m^$. 10 o o I'S 51 Analyst: Sis. Verified by: Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep mmi m^m m}:m:: ;:;:;:5;:;:; mmi^: Total QC 6.25% 1 0 b 1 12^ J7 0 6.25% 2 c o D 0 3 & o D (< if 0 to 4 c o D u 11 0 5 o ri 1 c •30 6 0 o tl |t4 0 3{ 7 o S o / c XI 8 f> o i'4 ll c 3c> 9 o o iK c 3r 10 O i i'^ D Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep 2 3 7 8 Total QC 12.5% 1 0 o 10 D 12.5% 2 0 , a <o 1 C 3 0 o 0 •3 Is ^ C 4 0 o D 0 5 o o b W c . Is-6 ri O 1"^ 0 7 5 o —s 0 •:;i 8 0 rl ,S o in 0 3<? 9 0 o o t w ' r:^ 0 10 0 O 0 C43 \^ 10 0 Time Fed (day): (01 \?.S0 (1) l&Ha (2) /V^^ (3)/^AA (4)jW^ (5) (6)J^Z) (7) - (8) :L Daphnid Survival and Reproduction Datasheet Test Species: C. c/t//)/a Start Date/Time: C-Z-O^ ( \^5ti End Date/Time: <gt.cUi.f Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep m^^m ;::i:|5;;;;: e Total QC 25% 1 0 ft 1 0 34 25% 2 0 & 5 IS 0 3 A 0 H ir 0 "ir 4 0 1^ 0 5 6 + <? c 3 i 6 0 T 0 7 n ; 1 • n 0 8 q b G) c /., 10 Cl 0 0 cS ..Cl ly I'D 'n 0 m Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep :1;;:;;; 2 imm mm\\ Total QC 50% 1 0 A S il a ?2-50% 2 0 0 (rt IT-<o ta 3 Ci <i a 4 r. ri 0 7 CJ 5 0 <D 0 ft ll 1^ Cj 6 fe-3 0 () 0 ZM- 7 0 3 0 15 0 a 2-(e 8 0 0 5 0 — V/J 9 0 0 S M 0 -z-r 10 0 0 0 6 Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep mm Total QC 100% 1 0 0 S 5 n 2-? 100% 2 0 .(?) 0 It. () •31 3 0 0 % Ilp •i? 4 0 c) il 5 0 0 r-^ 0 lu. 0 6 0 0 r\ 1^ /•/ 7 0 0 s 8 0 n. C~! 1^ 1 9 0 0 If — _ 10 0 6 0 3 —ti_ ^) 1 Notes: Comments: QC Check: Neonates for each replicate were blocked across concentrations at test initiation: d = dead; M = male; LIP = lost in progress E^s-s^i, -grt^ .rt-i.t^ OX'JA is^iVU 2. Au.e +0 1^^11 p-f .^^H f^.^•l,V^A•,•^>;. U/toi^S, 1^ \r;l-l,^l0b Final Review: <77^ "7/^/^C Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Fre9Twateffffroni??fc Client: Sample ID: Test No: ronic Bioassay Dudek Upstream Surface Concentration Day Lab Control *\ pH DO (mg/L) Cond. (pmhos/cm) Temp (°C) XM. U ^. 'XX :?n>itiai OS. ft. IX 1.H.O .2^ pH -1 •^"l DO (mg/L) S-.D 1-^ U—!—\ Temp (°C) Z5,\ I. M ( o -t>^i 1 ^ . ^ g, Concentration 6.25% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 pH m A)2. l,«i.H DO (mg/L) •1,5 n.ci •-7.-) -i • S n "A Cond. (|jmhos/cm) 'I He(<7 ' • ' Temp (°C) V.;.:,•:•;•:•.•.•:.:':':.l;^:--.14 ..3> . ...aH.\ pH hi nal:;sSfi;iSsi; '^'?/ I- '9^ DO (mg/L) 1.4 9r fs Temp (°C) 2-5.M •^4,<i C3.S.3 V • V .(lis Concentration 12.5% Day 0 1112 13 14 15 16 17 pH •^."^^ ""^ C,7S mi lat ft..O'Z-DO (mg/L) n.S "7. Si •l.G Cond. (Mmhos/cm) Temp (°C) •2-5. Z 2.H.\ pH :-:;:x:o;-:::::-:';:::S^ Fin dl li.oi = DO (mg/L) "S", .•.; ^ .0 Temp (°C) .2M .-1 Animal Source/Date Received Animal Age at Initiation: Comments: QC Check: Internal/NA 2?r hours Water Quality Measurements Test Species: C. dubia Start Date/Time: 6/2/2006 / End Date/Time: _Le_q_ Concentration 25% Dav 0 1 1 1 2 1 3 -1 4 1 5 1 6 1 7 pH ~1 -I "TS -iiKm ffl «ai 1.^1 DO (mg/L) ^.2 Cond: (pmhos/cm) 1.3=11 13.30 Temp (°C) rs.\ f: 2H,O 2.H.\ 2H.^ pH % ^•.^^•^ , = -nal DO (mg/L) i "1. 3 in 7.'I — Temp (°C) asj,<i Concentration 50% Day 0 1 1 1 2 1 3 1 . 4 1 5 1 6 1 7 pH "1.-1 T-"-\mm\ nil ~?SSD T -if? -7.-8 3 DO (mg/L) •-1.9 ^.2 Cond. (pmhos/cm) ^•200 Temp (°C) iH.5 •IH .U. l^ .:.:.;:':-:-:-:-:':':';'-tti-^. _ 1 2H.2 . •2M,.O pH rir 'B.s.'^ al ]Lr DO (mg/L) L..^: ^:| 8.1 Temp CC) 25.1 • ' ze - LP 1 Concentration Day 100% pH 1.U0 1.1 \ 11? DO (mg/L) •75.^ L L_ul Cond. (pmhos/cm) B-S-30 3^2/) Temp CC) Z-H.l 2M. S c5-/.b .•?H.:i 2H.!> pH Fil ialfSSiSss^ "S-.z-S DO (mg/L) Temp CC) ^3 •Z5. j 5iM-<^ X.5, Co Analysts: Initial: Yiu 5^ Final: 5A •Jrs ^1 Waufz/us Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego. CA 92121. Final Review: <J/Z "P///^^ Site: Downstream CETIS Test Summary Page 1 of 1 ReportDate: 05 Jul-06 3:30 PM Link: 03-4594-1949/0605-S065b Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Test No: 14-2485-4300 Start Date: 02 Jun-06 02:05 PM Ending Date: 09 Jun-06 01.40 PM Setup Date: 02 Jun-06 02:05 PM Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013 (2002) Dil Water: Diluted Mineral Water (8:2) Brine: Not Applicable Duration: 6d 23h Species: Ceriodaphnia dubia Source: In-House Culture Sample No: 15-4637-1306 Sample Date: 01 Jun-06 02:15 PM ReceiveDate: 02 Jun-06 12:00 PM Sample Age: 24h Material: Downstream Ambient Sample Code: 06-0464 Source: Dudek & Associates Station: Downstream Client: Project: Dudek & Associates Comparison Summary Analysis Endpoint NOEL LOEL ChV 16-5805-5249 7d Proportion Survived 100 > 100 03-5086-9758 Reproduction 50 100 MSDp Method N/A 70.711 N/A 25.18% Fisher's Exact Bonferroni Adj Wilcoxon Rank Sum Test Acceptability Analysis Endpoint Attribute 16-5805-5249 7d Proportion Sumved 03-5086-9758 Reproduction 03-5086-9758 Reproduction Control Response Control Response Statistic Acceptable Range Decision 1 31.5 0.8 15 - N/A N/A MSDp 0.25182 0.13-0.47 Passes acceptability criteria Passes acceptability criteria Passes acceptability criteria 7d Proportion Survived Summary Conc-% Control Type Reps Mean 0 6.25 12.5 25 50 100 Minimum Maximum SE SD CV Lab Control 10 10 10 10 10 10 1.00000 0.88889 1.00000 1.00000 1.00000 0.90000 1.00000 0.00000 1.00000 1.00000 1.00000 0.00000 ooooo ooooo ooooo ooooo ooooo ooooo 0.00000 0,11111 0.00000 0.00000 0.00000 0.10000 0.00000 0.33333 0.00000 0.00000 0.00000 0.31623 0.00% 37.50% 0.00% 0.00% 0.00% 35.14% Reproduction Summary Conc-% Control Type 0 5.25 12.5 25 50 100 Lab Control Reps Mean Minimum Maximum SE SD CV 10 31.5 26 35 0.8199 2.5927 8.23% 10 28.778 0 . 40 4.0476 12.143 42.19% 10 33.5 23 39 1.675 5.2967 15.81% 10 30.5 24 38 1.3764 4.3525 14.27% 10 27.6 0 38 3.4679 10.967 39.73% 10 25.4 18 33 1.3013 4.1150 16.20% 7d Proportion Survived Detail Conc-% Control Type Repl Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 0 6.25 12.5 25 50 100 Lab Control 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Rep 9 Rep 10 1.00000 N/A 1.00000 1.00000 1.00000 1-00000 Reproduction Detail OOOOO ooooo OOOOO ooooo OOOOO ooooo OOOOO ooooo ooooo ooooo ooooo ooooo 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0 6.25 12.5 25 50 100 % Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 Lab Control 32 32 34 35 31 26 30 30 34 31 0 39 25 27 36 35' N/A 26 31 40 33 38 27 23 39 36 33 36 31 39 31 38 29 25 24 36 28 32 31 31 39 31 29 34 25 21 38 33 32 0 28 36 28 26 24 18 33 23 25 22 29 26 000-089-125-1 CETIS™ V1.025B Analyst:. J C Approval: ZfL ifr/oc CETIS Analysis Detail Comparisons: Page 1 of 1 Report Date: 27 Jun-06 1:20 PM Analysis: 16-5805-5249/0605-S065b Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type 7d Proportion Survived Comparison Sample Link Control Link Date Analyzed Version 03-4594-1949 03-4594-1949 27 Jun-06 1:18 PM CETlSvl .025" Method Alt H Data Transform Fisher's Exact C>T Untransformed NOEL LOEL Toxic Units ChV 100 >100 MSDp 1.00 N/A Group Comparisons Control vs Conc-% Statistic Critical Lab Control Decision(0.05) 6.25 12.5 25 50 100 0.47368 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 1,00000 0.05000 Non-Significant Effect 1.00000 0.05000 Non-Significant Effect 0.50000 0,05000 Non-Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed 0 6.25 12.5 25 50 100 Lab Control Graphics 1.0: 0.9^ O.B 0,7 0.6: 0.5: 0.4 0.3. 0.2: 0.1 0.0 10 8 10 10 10 9 10 9 10 10 10 10 12.5 25 Conc-% 000-089-125-1 CETIS™ V1.025B Analyst $0 Approval: Z^J/O/ CETIS Analysis Detail Comparisons: Report Date: Page 1 of 1 27 Jun-06 1:20 PM Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analvzed Vpr<:ion Reproduction Comparison 03-4594-1949 03-4594-1949 27 Jun-06 1:19 PM CETISv1.025 Method Alt H Data Transform 2 NOEL LOEL Toxic Units ChV MSDp Bonrerroni Adj Wilcoxon Rank Sum C > T Untransformed 50 100 2,00 70.711 25.18% ANOVA Assumptions Attribute Test statistic Critical P Level Decision(O.OI) Variances Bartlett 28.66301 15.08628 0,00003 Unequal Variances Distribution Kolmogorov-Smirnov D 0.16073 1 0.13416 0,00063 Non-normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Decision(0.05) Between Error 418,6868 2897,855 83,73737 5 54,67652 53 1.53 0.19586 Non-Significant Effect Total 3316.5423 138,41389 58 Group Comparisons Control vs Conc-% Statistic Critical P Level Ties Decision(0.05) Lab Control 6.25 90 0.4841 6 Non-Significant Effect 12.5 124 0.9173 7 Non-Significant Effect 25 95 0.2406 4 Non-Significant Effect 50 98 0.3153 4 Non-Significant Effect 100 65 0.0008 5 Significant Effect Data Summary Original Data Transformed Data Conc-% Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD 0 LabControl 10 31.5 26 35 2.5927 6.25 9 28.778 0 40 12.143 12.5 10 33.5 23 39 5.2967 25 10 30.5 24 38 4.3525 50 10 27.6 0 38 10.967 100 10 25.4 18 33 4.1150 50-1 it 6.25 12.5 25 Conc-% 000-089-125-1 CETIS™ V1.025B Analyst:. Approval: 'J/t ?/(/(?/ Freshwater Chronic Bioassay Client/Sample ID: Test No: Dudek / Downstream Surface Conc. Rep Board/ Cup Daily Reproduction/ Survival Total QC Conc. Rep Board/ Cup mmiii m^m B\i^m m\4]i]\ ::;::S::;:; mmi i;;i|;8::i:: Total QC ConlrsL 1 H-J '-^^^ ConlrsL 2 w c— i 1 3 \fc -'UJi'+i- 4 H5 n / 5 HS i« 6 HS i^ 7 H5 8 H5 x\ 9 Hb 10 HS '--^ Analyst: SVV So Ac. Verified by: Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep \m^m mM;-. i;i;::3:;:;: ;:;:;:5:;;; mm\ Total QC 6.25% 1 0 o _ — _ ... o/ri 6.25% 2 0 o (I IS (••a, 3 0 17 ' 1 J - 1 -i-^ o 4 0 •n 11 1 -Zi-o ir\ 5 o O D /q o 6 f, Co 0 I'O o . 35-7 o i-)p 8 0 X(i 9 n r. s 3i 10 0 •0 r\ o 46 ' 1 Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep 1 i::;;;3i;:;;; 4 'mm^m\ :;:;:7::;;; 8 Total QC 12.5%, 1 0 o P ) Cn o 3} 12.5%, 2 0 0 )(.^ 0 3g-3 0 ri )^ 0 a.7 4 6 0 lb .0 a3 5 0 O 5 c 0 S"? 6 0 O »^ 0 i^ I<c 0 7 0 i:^ UD VI s J3 8 0 T) ,H o •Jtt) 9 0 O o 3i Ci 10 0 o 51 Time Fed (day): (0) 'vHoS: (1) ^CQ (2) 7^ (3)i^a2_ (4) jOl'Q (5) '^5 o (6). (7)_ (8)_ Daphnid Survival and Reproduction Datasheet Test Species: C. dubia Start Date/Time: ^.-q-bC ^\H&5 End Date/Time: (j,.^. Notes: Comments: QC Check: Neonates for each replicate were blocked across concentrations attest Initiation; d = dead; M = male; LIP = lost in progress . UVTSAA (oi^oS"- 3oii,<Jcm^ Final Review: s//2- Rep Daily Reproduction/ Survival QC Rep 1 m^M i^:^:;:4;:i: :;i;::5;;;: ::i:;7;::: ::::;;8;:;: 1 Otal QC 1 0 o s y (5 25% 2 0 n r ( » i^ (2 3 0 o H-o O :£'5' 4 c o 3 (X-O 5 0 0 i A 0 —( 6 0 S 0 h O 2.S' 7 G C) \\ O Z'L. 8 t) Q o o 31 9 t O s ic> o 10 0 o T Ift iH-o 3/ Conn Rep Daily Reproduction/ Survival Total QC Rep 1 :;;;:;3!:;:: ::;::i4;:::: fr mMw Total QC 1 Q H Z^-O 'Zl 50% 2 0 n 1 /•=r o 3 0 f—1 0 0 o 4 c o 0 lo ii o z-( 5 0 0 0 \ •^ 1^ o 3^ 6 6 o \ > 17 0 3-3 r) 7 c Q 0 o 8 0 ,\ 0 o o 9 0 0 0 o z-Y 10 D o -1 / c-o Conc: Rep ::;::i;:::;::: Daily Reproduction/ Survival Total QC Rep ii;i;2i:;:: •^i^H5;;i;: ::;;;S::;:| Total QC 1 c-t^ •n o 100% 2 0 r\ o IO e 2-(i> 3 6 O 0 9 C 4 0 0 3 0 Y 7 O 1^ 5 0 O T \^ /lb o/d , 6 0 0 H \o ^ O 7 0 O oL 1 0 0 7-S 8 0 o 0 lo 9 0 o s-Iri / --^ o 10 0 6 0 Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Fr^SwatenSfronic Bioassay Client: Sample ID: Test No: Dudek Downstream Surface Concentration Day Lab Control *| (^[^^ ^^ffe-f^'^iC) pH DO (mg/L) Cond. (Mmhos/cm) Temp CC) 7.M -7 ^ L> 3^ IS ZH.A pH DO (mg/L) l.lv ) Temp (°C) Concentration 6.25% Dav 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 pH n.^^|-| ^.i3 <&, il- DO (mg/L) Cond. (pmhos/cm) M-i5 us ?. LlaJ Temp (°C) xa.Cc v:;>:':-j;^:j pH ^.6i 00 hi ^.'^(^ nai \1 DO (mg/L) LM 1,-1 •S- 1 Temp (°C) —^ ^ 0 1 Concentration 12.5% Day 0|1|2|3|4|5|6|7 pH iRI 131 DO (mg/L) 7.C^ "7 .-I •1..'^ Cond. (pmhos/cm) 16^ (..(/•fCS if\'l-•106. It's Temp (°C) XH.5 2.H.\ pH F'm dl r. H DO (mg/L) -1.3 1.7 Temp (°C) •Z6,\ = -i-i 1—2 7^5. (£. Water Quality Measurements Test Species: C. dubia Animal Source/Date Received: Animal Age at Initiation: Comments: Internal/NA <l8'^ours start Date/Time: 6/2/2006 End Date/Time: Ip .q .pLe |\^MO Concentration 25% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 pH : ::;•;:: ;if "7.q^7 roa> "1 '\S DO (mg/L) • -l.io •n."i %--ci L: > "I.t, Cond. (pmhos/cm) il Iri^ 1 )S\ IZ02. '' Temp ("C) aM.3 24. i ^^,\ LILU pH r tnal 2 1 DO (mg/L) ; n: z "7.-7 ) - 0 • 2 1 Temp (°C) ; •Z^.H > Z"^ CP Concentration 50% Dav 0 1 1 1 2 i 3 1 4 1 5 1 6 1 7 pH oaf DO (mg/L) ?-i ^ Z t-1 ' * * Cond. (pmhos/cm) 7-106 )^H7 iiacbx> Temp (°C) •IH--T- :\\\-:-:-y.-y.::-y^i. •i^.l 2k X) pH Ft nai DO (mg/L) 1.3 5 1 Temp CC) A5.3 ' 1 fcZ Concentration 100% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 pH Ifll .jaf::?::?:::::;:::;;:.: •n DO (mg/L) 6 ^.5 <?. D Cond. (pmhos/cm) •5530 35,3o Temp (°C) .3 9M.3 ^ -1 ^H--^ pH •? -HC. Fin al DO (mg/L) ^.0 ' > ^ Temp (°C) ^-^.-^ i; A ~. . .- y L. 0 1 2 3 4 .<; 6 7 Analysts: Initial: k-sC 5b Final: >) rr -"3 QC Check: 10. Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Final Review: Site: Well #1 CETIS Test Summary Page 1 of 2 ReportDate: 05 Jul-06 3:31 PM Link: 11-4067-1023/0605-S065c Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (GA) Test No: 14-2485-4300 Test Type: Reproduction-Survival (7d) Duration 6d 21h Start Date: 02 Jun-06 02:25 PM Protocol: EPA/821/R-02-013 (2002) Species: Ceriodaphnia dubia Ending Date: . 09 Jun-06 12:10 PM Dil Water: Diluted Mineral Water 8:2) Source: In-House Culture Setup Date: 02 Jun-06 02:25 PM Brine: Not Applicable Sample No: 09-1629-1115 Material: Groundwater Client: Dudek & Associates Sample Date; 01 Jun-06 03:00 PM Code: 06-0461 Project: ReceiveDate: 02 Jun-06 12:00 PM Source: Dudek & Associates Sample Age: 23h Station: Well 1 Comparison Summary Analysis Endpoint NOEL LOEL ChV MSDp Method 09-2305-8525 7d Proportion Survived 100 > 100 N/A N/A Fisher's Exact 17-0630-4800 Reproduction 50 100 70.711 11.98% Bonferroni Adj t Point Estimate Summary Analysis Endpoint % Effect Conc-% 95% LCL 95% UCL Method 13-1322-4033 Reproduction 25 68.21970 63.18306 72.96908 Linear Interpolation 50 93.35859 85.34553 N/A Test Acceptability Analysis Endpoint Attribute Statistic Acceptable Range Decision 09-2305-8525 7d Proportion Survived Control Response 1 0.8 - N/A Passes acceptability criteria 13-1322-4033 Reproduction Control Response 32.7 15 - N/A Passes acceptability criteria 17-0630-4800 Reproduction Control Response 32.7 15 - N/A Passes acceptability criteria 17-0630-4800 Reproduction MSDp 0.11984 0.13 - 0.47 7d Proportion Survived Summary ' • Conc-% Control Type Reps Mean Minimum Maximum SE SD CV 0 Lab Control 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 6.25 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 12.5 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 25 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 50 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 100 10 0.90000 0.00000 1.00000 0.10000 0.31623 35.14% Reproduction Summary Conc-% Control Type Reps Mean Minimum Maximum SE SD CV 0 Lab Control 10 32.7 30 38 0.9781 3.0930 9.46% 6.25 10 33.667 30 37 0.9129 2.7386 8.13% 12.5 10 32 25 36 1.2247 3.6742 11.48% 25 10 34.2 31 38 0.786 2.4855 7.27% 50 10 30.9 28 36 0.7371 2.331 7.54% 100 10 14.4 0 21 1.9276 6.0955 42.33% tice :/. 000-089-125-1 CETIS™ V1.025B Analyst: •f C Approval: 7/?/V' CETIS Test Summary Report Date: Link: Page 2 of 2 05 Jul-06 3:31 PM 7d Proportion Survived Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 1-00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 6.25 1.00000 N/A 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 12.5 1.00000 N/A 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 50 1.00000 1-00000 1-00000 1.00000 1,00000 1-00000 1.00000 1.00000 1.00000 1.00000 100 1.00000 0.00000 1-00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Control 32 30 30 31 30 35 34 38 30 37 6.25 30 N/A 35 31 33 30 36 37 35 36 12.5 36 N/A 34 25 30 35 35 33 32 28 25 33 34 32 31 32 33 36 38 35 38 50 30 29 29 31 31 30 32 33 28 36 100 21 0 14 14 14 21 13 11 18 18 000-089-125-1 CETIS™ V1.025B Analyst:. Approval:'7/^ j/e/cKT CETIS Analysis Detail Comparisons: Page 1 of 1 Report Date: 27 Jun-06 1:27 PM Analysis: 09-2305-8525/0605-S065c Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type 7d Proportion Survived Comparison Sample Link Control Link Date Analyzed Version 11-4067-1023 11-4067-1023 27 Jun-06 1:26 PM CETlSv1.025~ Method Alt H Data Triansform Fisher's Exact C>T Untransformed NOEL LOEL Toxic Units ChV 100 MSDp >100 1.00 N/A Group Comparisons Control vs Conc-% Statistic Lab Control Critical Declsion(0.05) 6,25 12,5 25 50 100 1,00000 1,00000 1,00000 1,00000 0.50000 0.05000 0.05000 0,05000 0,05000 0.05000 Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Data Summary Conc-% Control Type 0 6,25 12,5 25 50 100 Non-Responders Responders Total Observed Lab Control 10 9 9 10 10 9 10 9 9 10 10 10 Graphics 1.0^ 0.9. .> 0.8: m 0.7: •J 0.6: I 0,5^ a •a 0,4 rs 0,3. 0,2: 0,1. 0,0 12,5 25 Conc-% 000-089-125-1 CETIS™ v1,025B Analyst: ^ Approval: I/T/OB CETIS Analysis Detaii Comparisons: Report Date: Analysis: Page 1 of 1 27 Jun-06 1:27 PM 17-0630-4800/0605-S065C Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 11-4067-1023 11-4067-1023 27 Jun-06 1:27 PM CETISv1.025 Method Alt H Data Transform Bonferroni Adj t C >T Untransformed NOEL LOEL 50 100 Toxic Units 2.00 ChV MSDp 70.711 11.98% ANOVA Assumptions Attribute Test Statistic Critical P Level Decision(O.OI) Variances Bartlett 12.67539 15,08628 0.02662 Distribution Kolmogorov-Smirnov D 0.09477 Equal Variances 0,13527 0,20600 Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Decision(0.05) Between 2835,431 567.0862 5 42,55 0,00000 Significant Effect Error 693 13,32692 52 Total 3528,43115 580.41311 57 Group Comparisons Control vs Conc-% Statistic Critical P Level MSD Decision(0.05) Lab Control 6.25 -0.5763 2.40023 0,7166 4,02599 Non-Significant Effect 12.5 0.41733 2,40023 0.3391 4,02599 Non-Significant Effect 25 -0,9188 2,40023 0.8188 3,91861 Non-Significant Effect 50 1,10254 2,40023 0,1377 3,91861 Non-Significant Effect 100 11,2091 2.40023 0,0000 3,91861 Significant Effect Data Summary Original Data Transformed Data Conc-% Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD 0 Lab Control 10 32.7 30 38 3,0930 6.25 9 33,667 30 37 2,7386 12,5 9 32 25 36 3.6742 25 10 34,2 31 38 2,4855 50 10 30,9 28 36 2.331 100 10 14.4 0 21 6.0955 Graphics I .{ } { I. Reject Null I? c 3 12.5 25 Conc-% -0.5 0.0 O.S Rankits 000-089-125-1 CETIS™ V1.025B Analyst: Approval: if^^Dd CETIS Analysis Detail Ceriodaphnia 7-d Survival and Reproduction Test Linear Interpolation: Page 1 of 1 Report Date: 27 Jun-06 5:39 PM Analysis: 13-1322-4033/0605-S065c 000-089-125-1 CETIS™ V1.025B Analyst:_ Approval.17^ "^^/^ ^ Freshwater Chronic Bioassay Client/Sample ID: Test No: Dudek/Well 1 Conc. Lab Control Rep Board/ Cup 9 Analyst: Verified by: HS 4.5 21 28 2a .3£i. 32 11 !;l;;i; o o 0 Daily Reproduction/ Survival -L2_ CXJ Cb _LL -LiL J-2_ -L3_ /2_ ' cD ;:::7 l_L 0 1^ V I .AO Total 30 3a_ 32. QC Conc. 25% Rep 7 8 9 TIT Daphnid Survival and Reproduction Datashee! Test Species: C. dt;Jb/a Start Date/Time: / \'^-3<, End Date/Time: [ _2_ o XL o XI. Daily Reproduction/ Survival ''-'•^•y^mm-mm-wsmmi^^ to O 0 o S _LL J5L -1^ J5_ a XL i6. 0 1 s a IS Total 23^ 12^ 32- 11. 3£. QC 25: Conc. 6.25% Conc. 12.5% Rep 8 9 TIT Rep 5 6 9 "IF o Daily Reproduction/Survival ' •' ^3:i:|:j;;!;;;4;::;;|;:::::5::n;l;:^:;;& D L? T5~ "If- TT" LO s: IK IS: TT Total 20 3y 317 12- 3& QC 77" Conc. 50% IL _22_ _ Daily Reproduction/ Survival O "TT O -U- A5L -1^ 43^ _i2i- -iii- If 0 i2. Total lk. 3 c? 251 3^ QC Rep 9 IF Time Fed (day): (Q)\^.^ (D ^AV^ (2)^3,573 (3)J^^ (4) /' / Q (5) 1500(61...^^^ (7) (8). Notes: Comments: QC Check: JX. o Dally Reproduction/Survival Til JL. 5 IL Ji. t: Jl J2_ il 6 Total 52 lis: Neonatgsfoj^ach^licate ^^^^ ^,^^3^. ^ = ,^ale: LIP = lost in_pmnr»^ ^^::iri^A.c\tV ,a^X-V ^; :^^v.^-H •v,.,^^^, '..o.^ •^.-^x...^ . Final Review: ^/S- 7/^/ Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. QC Conc. Rep Daily Reproduction/ Survival QC Rep i;:;::1;;;:: m2\i\: M^\im ::;:;!<:;;; ^:mm\ Total QC 100% 1 2 0 0 o <:)/<-v 1 c? /O — y J . 3 —#9— o o fi (n I'i 4 n o n ' < 5 0 • o ... o V ? —t T _L_J / V 6 tl 0 3 0 il 2 ) / A 7 0 o o 0 9 i I -UJ 8 0 , , r) o O '&~ 1 ( •-^•mi^]^^; 9 10 0 c u 0 a o 1 .5 2, <? o iy— ll —u 1^ Fr^WWateWB'oniPBRjasfl^^ Client: Sample ID: Test No: Dudek Well 1 Cher Concentration Day Lab Control ^ 3 I 4 1 5 pH DO (mg/L) Cond. (pmhos/cm) Temp rC) ISfL t^z pH DO (mg/L) Temp (°C) -1 96.-1 US •7.L0 (CiS •I.Z FInaj ifil 2.HA -2x1 ^. I 25-. So Concentration 6.25% Dav 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 pH m -) u2 mm ^. 5H 1.S2. DO (mg/L) -\LJ> -!.•>. -^.^ 1.8 Cond. (pmhos/cm) Mm Temp (°C) ZH.-*- .;.-:-.;.'.-.:.-:\':-:-;:-f:; -) ^ ^ 2.H .\ pH -1.%%' FJ naJ::::S:i:;::::::H;: 1 .9-^: DO (mg/L) ^. 1 i-U: Temp (°C) M! 'JfSr^ . (-^ '^:) I Xi X- Concentration 12.5% Day 0 1 1 1 2 1 3 1 4 1 5. 1 6 1 7 pH ^•.)) mi iiai 3 -3. DO (mg/L) 1. 7-~1. 5 «.o •. —-r.-^.^ "I K Cond. (pmhos/cm) 'u>5^i IOCP ^•7r, —> Temp CC) •IH.-^ •J-M .1 2H.0 —t—LJl 2-^.\ pH Fir al DO (mg/L) "I .5 -7.-5 ^.^ Temp CC) •2-5 M < &S,3 25-1 ^^•'^ 1 . LP Animal Source/Date Received: Animal Age at Initiation: Comments: QC Check: Internal/NA <lLViours 9-^ <i>L-n\oU Water Quality Measurements Test Species: C. dubia Start Date/Time: 6/2/2006 End Date/Time: (i2/^/o& /Z-/0 Concentration 25% Dav 0 1 1 1 2 1 3 1 4 1 5 1 6 1 pH ^.13.- roa? 7.0s DO (mg/L) 1-H • •^. '1 - -—14— a? Cond. (pmhos/cm) ..1XH i:a^M L.:—1 I"30C) Temp (°C) ^,:;k ::•:•:•:•:•:•:•:•:•;•:-:•:•]«-• 1 XH.0 2H.C) pH hi nal 7-91 7^ DO (mg/L) -1.(.v> ^. 1 1 Temp (°C) 25-1 Concentration 50% Dav 0|1|2|3|4|5|6|7 pH n.ni i(i« tai -I.'i 5 Q).1b DO (mg/L) -I.CB 0 <, Cond. (pmhos/cm) Z056 Temp CC) ^M. \ c5Al.D #^ ZH.© pH rtr 7'>*:^ al 1 DO (mg/L) n.L. ^. 1 § Temp (°C) 1 9-4.c, Concentration Day 100% pH 1- •S'T, -1 . •! •O- Ul 1.-7 <1 G..3^ DO (mg/L) -l.O) •3.H Cond, (pmhos/cm) ^Lz>MO SLJSa> Temp CO U-M-.H 6iy,e> •2H-0 pH 7 3^5 Fl tal 7.'? Z 7~7<^ DO (mg/L) -l.-l B". ( —1 1 ^ H - /•A 1,,- ^ ? s. \ Temp (°C) 25,1 2-5. U> Analysts; Initial: Final: 0 1 2 3 4 6 7 Analysts; Initial: Final: *-LC Analysts; Initial: Final: S5 ^t^s '•j WauWus £nv/ronmen(a;, LLC. 5550 Morehocyse OriVe, Suite 150 San Diego, CA 92121. Final Review: UT^ Site: Well #3 CETIS Test Summary Report Date: Link: Page 1 of 2 05 Jul-06 3:47 PM j Ceriodaptinia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Test No: 14-2485-4300 Test Type Reproduction-Survival (7d) Duration: 6d 23h StartDate: 02 Jun-05 02:40 PM Protocol: EPA/821/R-02-013 (2002) Species: Ceriodaphnia dubia Ending Date: 09 Jun-06 01:55 PM Dil Water: Diluted Mineral Water (8:2) Source: In-House Culture Setup Date: 02 Jun-05 02:40 PM Brine: Not Applicable Sample No: 15-4275-6942 Material: Groundwater Client: Dudek & Associates Sample Date: 02 Jun-06 09:30 AM Code: 06-0462 Project: Receive Date: 02 Jun-06 12:00 PM Source: Dudek & Associates Sample Age: 5h Station: Well 3 Comparison Summary Analysis Endpoint NOEL LOEL ChV MSDp Method 10-9077-9090 7d Proportion Survived 100 > 100 N/A N/A Fisher's Exact 04-9533-3696 Reproduction 25 50 35.355 10.96% Dunnett's Multiple Comparison Point Estimate Summary Analysis Endpoint % Effect Conc-% 95% LCL 95% UCL Method 14-8920-7910 Reproduction 25 55.81551 46.23579 61.52432 Linear Interpolation 50 77.67380 71.68674 82.02247 Test Acceptability Analysis Endpoint Attribute Statistic Acceptable Range Decision 10-90/7-9090 7d Proportion Survived Control Response 1 0.8 - N/A Passes acceptability criteria 0-1-9533-3696 Reproduction Control Response 32.7 15 - N/A Passes acceptability criteria •14-8920-7910 Reproduction Control Response 32.7 15- N/A Passes acceptability criteria 04-9533-3696 Reproduction MSDp 0.10963 0.13-0.47 (A) foilo oeceplubility ciitciiu 0^ ^Ji- 7d Proportion Survived Summary Conc-% Control Type Reps Mean Minimum Maximum SE SD cv 0 Conductivily C 10 0.40000 0.00000 1.00000 0.16330 0.51640 129.10 0 LabControl 10 1.00000 1-00000 1.00000 0.00000 • 0.00000 0.00% 6.25 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 12.5 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 25 10 1.00000 1.00000 1.00000 0.00000 0.00000 0.00% 50 10 0.90000 0.00000 1.00000 0.10000 0.31623 35.14% 100 10 0.90000 0.00000 1.00000 0.10000 0.31623 35.14% Reproduction Summary Conc-% Control Type Reps Mean Minimum Maximum SE SD cv 0 Conductivity C 10 4.5 0 14 1.9394 6.-1328 136.28 0 LabControl 10 32.7 30 38 0.9781 3.0930 9.46% 6.25 10 31.3 25 35 1.0440 3.3015 10.55% 12.5 10 30.9 27 36 0.9713 3.0714 9.94% 25 10 32.6 30 34 0.4269 1.3499 4.14% 50 10 25.7 17 33 1.6265 5.1435 19.26% 100 10 8 0 12 1.2472 • 3.9441 49.30% CK. sMiSi-r'cc:/ c^i(Terence.. Ce,, ^pA 1 . J r^y , / , ^ • iet t=H/f ciii>tiym^<^f &^l /lt.-o-L~ oi2 (l-ocz) ho 000-089-125-1 CETIS™ V1.025B Analyst:. Approval: '^/^ I CETIS Test Summary Page 2 of 2 ReportDate: 05 Jul-06 3:47 PM Link: 09-1379-7450/0605-S065d 7d Proportion Survived Detail ... Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 0.00000 0.00000 1.00000 1.00000 1.00000 1.00000 0.00000 0.00000 0.00000 0.00000 0 Lab Control 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 6-25 1.00000 1.00000 1.00000 1.00000 1.00000 1,. ooooo 1.00000 1.00000 1.00000 1.00000 12.5 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 50 1.00000 0.00000 1 ooooo 1.00000 1.00000 I'.OOOOO 1.00000 1.00000 1.00000 1.00000 100 1.00000 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 0 0 10 7 14 14 0 0 0 0 0 Lab Control 32 30 30 31 30 35 34 38 30 37 6.25 34 28 29 35 35 31 33 30 33 25 12.5 27 34 28 32 36 27 32 31 33 29 25 31 34 33 32 34 30 33 34 32 33 50 31 17 25 23 22 26 32 27 33 31 100 8 11 0 9 12 5 12 8 4 11 000-089-125-1 CETIS™ V1.025B Analyst:. Approval:'^ I I I CETIS Analysis Detail Comparisons: Report Date: Analysts: Page 1 of 1 27 Jun-06 1:39 PM 10-9077-9090/0605-S065d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version 7d Proportion Survived Comparison 09-1379-7450 09-1379-7450 27 Jun-06 1:38 PM CETlSvl .025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Fisher's Exact C>T Untransformed 100 >100 1.00 N/A Group Comparisons Control vs Conc-% statistic Critical Decision(0.05) Lab Control 6,25 12,5 25 50 100 1,00000 1.00000 1.00000 0.50000 0.50000 0,05000 0,05000 0,05000 0.05000 0,05000 Non-Significant Effect Non-SignificanI Effect Non-Significant Effect Non-Significant Effect Non-Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed 0 Lab Control 10 0 10 6.25 10 0 10 12.5 10 0 10 25 10 0 10 50 9 1 10 100 9 1 10 Graphics 1.0-o o G O 0.9- •D o o s .| 0.8-W 0.7- I 0.6- 1 0.5- TJ 0,1- 0,3- 0,2- 0,1- 0,0^ 0,0^ 0 6,25 12,5 25 Conc-% 1 50 100 000-089-125-1 CETIS™ V1.025B Analyst:. Approval: CETIS Analysis Detail Comparisons: Page 1 of 1 ReportDate: 27 Jun-06 1:39 PM Analysis: 04-9533-3696/0605-S065d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Reproduction Comparison Sample Link Control Link Date Analyzed Version 09-1379-7450 09-1379-7450 27 Jun-06 1:39 PM CETISv1.025" Method Alt H Data Transform Z NOEL LOEL Toxic Units ChV MSDp Dunnett s Multiple Comparison C > T Untransformed 25 50 4.00 35.355 10.96% ANOVA Assumptions Attribute Test Statistic Critical P Level Decision(O.OI) Variances Bartlett 13,29399 15,08628 0,02077 Equal Variances Distribution Kolmogorov-Smirnov D 0.05067 0,13307 1,00000 Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F Statistic P Level Decision(0.05) Betw/een 4586,333 917,2667 5 74.64 0,00000 Significant Effect Error 663,6 12,28889 54 Total 5249,93347 929,55555 59 Group Comparisons Control vs Conc-% statistic Critical P Level MSD Decision(0.05) Lab Control 6,25 0,89301 2.28667 > 0.0500 3,58488 Non-Significant Effect 12.5 1.14816 2.28667 > 0.0500 3,58488 Non-Significant Effect 25 0,06379 2.28667 > 0.0500 3,58488 Non-Significant Effect 50 3,82719 2.28667 < = 0.0500 3,58488 Significant Effect 100 15,7553 2.28667 < = 0,0500 3,58488 Significant Effect Data Summ ary Original Data Transformed Data Conc-% Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD 0 Lab Control 10 32,7 30 38 3,0930 6.25 10 31.3 25 35 3,3015 12.5 10 30.9 27 36 3.0714 25 10 32,6 30 34 1.3499 50 10 26.7 17 33 5.1435 100 10 8 0 12 3.9441 Graphics I 3(H a u 1 .f{. Reject Null SB 12.5 25 Conc-'Vo -1.0 -0.5 0.0 0,5, 1,0 1,5 2.0 2.5 Rankits 000-089-125-1 CETIS™ V1.025B Analyst:. Approval: 0/^ 7, CETIS Analysis Detail Linear Interpolation: Page 1 of 1 Report Date: 27 Jun-06 5:35 PM Analysis: 14-8920-7910/0605-S065d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Reproduction Analysis Type Linear Interpolation Sample Link Control Link Date Analyzed Version 09-1379-7450 09-1379-7450 27 Jun-06 5:34 PM CETlSvl.025 Linear Interpolation Options X Transform Y Transform Seed Linear Linear Resamples Expanded CL Method 7055475 200 Yes Two-Point Interpolation Point Estimates % Effect Conc-% 95% LCL 25 50 95% UCL 55.81551 77.67380 46.23579 71,68674 61,52432 82,02247 Data Summary Calculated Variate Conc-% Control Type Count Mean Minimum Maximum SE SD 0 Lab Control 10 32.7 30 38 0.63136 3,09300 6.25 10 31,3 25 35 0.67392 3.30151 12,5 10 30,9 27 36 0.62694 3.07137 25 10 32,6 30 34 0.27555 1.3499 50 10 26,7 17 33 1.04991 5.1435 100 10 8 0 12 0,80508 3.94405 Graphics 000-089-125-1 CETIS™ V1.025B Analyst: '^C- Approval: 0"/^ Freshwater Chronic Bioassay Client/Sample ID: Test No: Dudek/Weil 3 Conc. Rep Board/ Cup Daily Reproduction/ Survival Total QC Conc. Rep Board/ Cup m-mm m2\\ih mm\ m^m mm-i m^m H:ii:8i:Hi Total QC ^^^st^ab ConfroO^ 1 H5 ^H ^^^st^ab ConfroO^ 2 HS fri /I r 3 H.5 ^ 2^ L/x\ -Wu( - 4 HS ^r^ 1 5 H-J sR 6 <^ HO 7 HS H? 8 H5 9 HM 10 Analyst: A< UJI Verified by: Daphnid Survival and Reproduction Datasheet Test Species: C. dubia Start Date/Time: (,-2-C)4 / H'A.D End Date/Time: U.A.CIP / Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep mmtA i;;:;2;;:;; ::::;:3;i;:; m^m ::::::5;;:;; :i:;:!S;;:;: \^\mm ::;i:;8::::; Total QC 25% 1 0 c-> \^ ?i 25% 2 0 o • ^ ''^ r) \i 3H' 3 n o D ••? iH ?J 4 o M o J^- 5 C rr, 0 o J'Y 6 c, (D o lp i o 30 7 0 <Q 1^ •2. 3^ 8 o 0 7 i'^ iH o 9 Q Ci v-6^ H IT- 10 - 0 o 0 VJ a Conc. Rep Daily Reproduction/ Survival QC Conc. Rep mmm mmm i;:-:i:i*;i;; mm &m mim n:;;;8;;;;; 1 0T3t QC 6.25% 1 o o o Iv r7 6.25% 2 c o o '\ \1 • 3 t 6 Q il 0 4 o 0 o '7 0 "sr 5 c o o o 6 0 o 1^ o 7 o PJ o 11/ c 8 6 o o o -\-b 9 Q 0) o n 10 O O 0 f 3 is Conc. Rep Daily Reproduction/ Survival Total QC Conc. Rep m^-mm^ ft::;;; -•mm ;;;i;8;;;;; Total QC 12.5% 1 0 o -o 5 iQ O 12.5% 2 0 O 1^ 3 c i'U o •vO if— I/:! 4 0 CJ M o 15 5 r. • o 30 6 0. •o 1 / —( 1.—— c 7 0-"5 — '4 M c ^ 8 C: o I -1? n •iM 9 r. rj o \v o i 3^ 10 0 o c> \o o \1 2*^ Time Fed (day): (0) \HHO (1) (2)J^i^ (31 (4) ii/< (5)_l5lQ_ (6) 1 (7)_ (8)^^ Rep Daily Reproduction/ Survival Total QC Rep mmm ;;:;;2:;;; ;:;;;;3;;;;; ^i;!;;*!;;; ;;;;;:5;;;;; m^^mi ;i;i;7;;;;; i:;;;*;;;; Total QC 1 o <» o 1. -to i} ?( . 50% 2 c o 0 M & 3 o 03 • o } 1 < c? i| -U -4 V o o P IT' o 5 6 ^ O '\1^ •a L£) 6 0 o % •tie, 7 CJ o If (.> •VZ-72. 8 O o I'V-o ^7 9 10 6 o (i 11 73 c l> ^ i> "Si Hnnr Rep Dally Reproduction/ Survival Total QC Rep m^^im ;;;;;2:;;;; ;;-!;i3;;;;;; ;;;;;?*;;;;; ;;;;;;5;;;;: ;;;;;;6;;;i;; ;;;;;?;;;;; ;;;i;;8;;;!;. Total QC 1 r-. 0 o 0 0 M ? 100% 2 a o o 0 3 /I 3 O 6 • o Oil 4 c C ?'. 4 5 0 o ^ ? 0 6 ^: 0 o X 0 3 r 7 0 n y ti 5 /2- 8 C o 0 f <£> M 9 c. 0 O 0 t O .^-y 10 D 0 } T) X .5 /f 1 Notes: Comments: QC Check: Neonates for each replicate were blocked across concentrations at test initiation; d = dead; M = male; LIP = lost in progress Final Review: \i fl^ 7/^/oC Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Freshwater Chronic Bioassay Dudek Client: Sample ID: Test No: Well 3 Concentration LabC -ontrol *!, /g^ ! 4 1 5 Day 0 1 1 1 2 1 3 -ontrol *!, /g^ ! 4 1 5 1 6 1 7 ^ ::g:':fi :tit:.vt::^-ti:t:.:tt-tfttttttt t.t:^yyyttr> Ihl:ial « f-i pH %, vn •^.1?) ^^\^ : 9. DO (mg/L) "-i.vr> -l-t Cond. (pmhos/cm) Temp (°C) 51-1 y —1—&— •3U,.\ -•••••-••• t. .tmi-tmtmmt itvmitmtMi mmmt^mitttttttmtttttmttttmmXiiiai^^ pH •n.B<7 1 ^'o DO (mg/L) ^-^ <, ^•1 Temp (°C) 25.1 S2.^. :;^S ,'7 Concentration 6.25% Day 0|l|2|3|4l5l6l7 Jiiitiat pH 1. -n .-1 a T. DO (mg/L) -1.3 1~\ Cond. (pmhos/cm) s^o Temp CO a.<-t.\ 5:S.^1 2.H.I :v::v^.v"'-'-:v-"::¥::¥:¥;:.->.:.->^ -ti-^-^-^^t-tmi:: <<f: <:xf: .y. mmmttMiTiiiitmmtt^mt^i^mit:;^^^ pH ^. 10 DO (mg/L) nM 1-Temp rC) Concentration 12.5% Day 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 Initial pH 1 sc> 1. JO DO (mg/L) l.^ 7.7-5i—1 7.a Cond. (pmhos/cm) iij>q lo\q Temp CC) A>-V3 •iH . 0 ^->tmmmt;mm mmmmm mmmtmt: Mt tm^^ma mmmaim mtmmtttmmti mm -trnm tmt pH •7.^1^-f .IU DO (mg/L) •7.1/ Temp (°C) 29.) ' 54.^ ^.:-7 7-5 ..U' Animal Source/Date Received: Animal Age at Initiation: Comments: QC Check: Internal/NA 2H <'8. hours Sc^vy.^v^ »<.vg.V<A ^y'Cov •\n :)^v\iUAu)X -t- (i^ A.&.v|^ I •VVy<i-.u^K n Water Quality Measurements Test Species: C. dubia Start Date/Time: 6/2/2006 / ^v\^ o End Date/Time: .g; .^^^ j Concentration 25% Day 0|l|2l3l4l5l6)7 mtttmmmmmmm mm t;-mttmimmmttmmtt:ttmtttttm:tmm:tfttt^ mmmmt;tmttttm tmmtmttm m m pH •^. n \ -1.33) 7.-:^tr DO (mg/L) •"1 -.I -1.5-7.M I.Cc, l.<=\ Cond. (pmhos/cm) V'^\9 Temp (°C) 3-H.\ tH-3 mo Final pH ?-27 DO (mg/L) •1.H •?.l Temp CC) n Concentration 50% Day 0|l|2|3|4|5l6l7 InHial pH •n.so " 7,<5c^ % . IS "I.IH DO (mg/L) •-1.9 -1.-1 Cond. (pmhos/cm) •l"T?rO 2500 Temp (°C) •;tM.^ c^V)0 2M-0 :•: :'*•: mffmmitmtmm:vmttmtt:ttttmmtmtm:tf:mmm pH B. 1 ^• &,u DO (mg/L) -l.-S" •3,3 &'.0 •5.0 ^/ S.2- Temp (°C) ^5,3 2$.( ' as n Concentration 100% Day 0|l|2|3|4|5|6l7 Initial pH i.Ln 3- VO DO (mg/L) 7.q <5 \ •8,0 Cond. (pmhos/cm) M.7CP HiilO Temp CC) ZH.0 'i' mstm ;:;:; mm:mmmm imii mmtmti ^mimmi t ;::;;fitnai;;;i;; mtm mtttmm ttt:mtmmtttmmtm pH ^: /a "^"^ IS' 17 DO (mg/L) S'.l Temp CC) •7.s.^ ^ •'h Z5.\ >L-^-^ . •15. U Analysts; Initial: Final: 56 1-^ ^ i£\>"7\ Nautilus Environmental, LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Final Review: J /g^ 7/^^~/0^ Conductivity Controls CETIS Test Summary Ceriodaphnia 7-d Survival and Reproduction Test Page 1 of 2 Report Date: 05 Jul-06 3:44 PM Link: 03-4627-6794/0605-S065a Test No: Start Date: Ending Date: Setup Date: Nautilus Environmental (CA) 14-2485-4300 02 Jun-06 02:40 PM 09 Jun-06 02:10 PM 02 Jun-06 02:40 PM Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013 (2002) Oil Water: Diluted Mineral Water (8:2) Brine: Nol Applicable Duration: 6d 23h Species: Ceriodaphnia dubia Source: In-House Culture Sample No: 02-5879-7542 Material: Sample Date: 01 Jun-06 12:45 PM Code: ReceiveDate: 02 Jun-06 12:00 PM Source: Sample Age: 26h Station: Upstream Ambient Sample 06-0463 Dudek & Associates Upstream Client: Project: Dudek & Associates Comparison Summary Analysis Endpoint 08-4195-7466 7d Proportion Survived 03-3598-3655 Reproduction ^L<v4 Cor\-trvl us. 6?M<^t/Cl^/V.Vy Cc?Mr<, l] Cf ic^cc-l^jVyV-y =J^£00 ^MSDp NOEL LOEL ChV Test Acceptability Analysis Endpoint N/A 12.34% Method Fisher's Exact Equal Variance t SilKtiS'hi'c«.y\ Attribute 08:4195-7466 7d Proportion Survived 03-3598-3655 Reproduction 03-3598-3655 Reproduction 03-3598-3655 Reproduction Control Response Control Response Control Response Statistic Acceptable Range Decision 1 31.5 20 0.8 - N/A 15 - N/A 15-N/A MSDp 0.12344 0.13 - 0.47 7d Proportion Survived Summary Conc-% Control Type Reps Passes acceptability criteria Passes acceptability criteria Passes acceplability criteria ^^^-feuli acceptability oriteria-' gi^f, Sc 0 0 6.25 12.5 25 50 100 Mean Conductivily C Lab Control 10 10 10 10 10 10 10 1.00000 1.00000 1.00000 1.00000 1.00000 0.90000 0.90000 Minimum Maximum SE SD CV OOOOO ooooo OOOOO ooooo OOOOO ooooo 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 0-00000 0.00000 0.00000 0.00000 0.10000 0.10000 0.00000 0.00000 0.00000 0.00000 0,00000 0,31623 0,31623 0,00% 0.00% 0.00% 0.00% 0.00% 35.14% 35.14% Reproduction Summary Cor\c.% ^Control Type Reps Meaj: 0 0 6.25 12.5 25 50 100 Conductivity C Lab Conlrol Minimum Maximum SE SD 10 20 / 8 27 10 26 35 10 31.3 27 36 10 29.9 25 34 10 32.1 27 37 10 26.2 6 35 10 24.6 31 cv 2.087 0.8199 0.8699 1.0899 0.9481 2.5768 1.4772 6.5997 2.5927 2.7508 3.4464 2.9981 8.1486 4.6714 33.00% 8.23% 8.79% 11.53% 9.34% 31.10% 18.99% sfec-Z/b/^ 10,Z.S: Z.S 000-089-125-1 CETIS™ V1.025B Analyst: Approval: I I I I I I I CETIS Test Summary Report Date: Link: Page 2 of 2 05 Jul-OS 3:44 PM 7d Proportion Survived Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5. F!ep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0 • Lab Control 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 6.25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 12.5 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 50 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 1.00000 1.00000 100 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 0.00000 1.00000 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 15 11 8 24 27 25 26 20 20 24 0 Lab Control 32 32 34 35 31 26 30 30 34 31 6.25 36 33 30 32 30 31 27 30 35 29 12.5 34 25 33 33 25 33 31 30 27 28 25 34 30 35 37 31 34 29 32 32 27 50 32 32 32 35 24 26 26 6 25 24 100 23 . 31 28 26 21 28 25 26 14 24 000-089-125-1 CETIS™ V1.025B Analyst: -^C- Approval: 7"/^ l/'f/o^ CETIS Analysis Detail Ceriodaphnia 7-d Survival and Reproduction Test Comparisons: Page 1 of 1 Report Date: 27 Jun-06 12:01 PM Analysis: 08-4195-7466/0605-S065a Nautilus Environmental (CA) Endpoint Analysis Type 7d Proportion Survived Comparison Sample Link Control Link Date Analyzed Version 03-4627-6794 03-4627-6794 27 Jun-06 12:00 PM CETlSvl.025 Method Alt H Fisher's Exact C>T Data Transform Untransformed NOEL LOEL Toxic Units ChV 0 >0 MSDp N/A N/A Group Comparisons Control vs Control Statistic Critical Lab Control Conductivity Co 1.00000 Decision(0.05) 0.05000 Non-Significant Effect Data Summary Control Type Non-Responders Responders Total Observed 0 Conductivity Co 10 0 0 LabControl 10 0 Graphics 1.0 0.9- .> 0.8- 5 0.7- e 0.6- 0.5- 0.4-; 0.3-. 0.2 0.1- 0,0 10 10 (CO) Conc-Vo (LC) 000-089-125-1 CETIS™ V1.025B AnalysL Approval:'^/^ 7^/o^ Comparisons: Page 1 of 1 CETIS Analysis Detait Report Date: 27 Jun-06 12:01 PM Analysis: 03-3598-3655/0605-S065a Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version Reproduction Comparison 03^627-6794 03-4627-6794 27 Jun-06 12:00 PM CETISv1.025 Method Alt H Data Transform Z NOEL LOEL Toxic Units ChV MSDp Equal Variance 1 C > T Untransformed <0 0 N/A 12.34% ANOVA Assumptions Attribute Test Statistic Critical P Level Decision(O.OI) Variances Variance Ratio 6,47934 6.54109 0.01034 Equal Variances Distribution Shapiro-Wilk W 0,93058 0.86826 0.15807 Normal Distribution ANOVA Table Source Sum of Squares Mean Square DF F statistic P Level Decision(0.05) Between 661,25 661,25 Error 452,5 25,13889 1 26.30 18 0.00007 Significant Effect Total 1113,75 686,38889 19 Group Comparisons Control vs Control Statistic Critical P Level MSD Decision(0.05) LabControl Conductivity Co 5,12873 1.73406 0.0000 3.88824 Significant Effect Data Summary Original Data Transformed Data Conc-% Control Type Count Mean Minimum Maximum SD Mean Minimum Maximum SD 0 Conductivity C 10 20 8 0 LabControl 10 31.5 26 27 35 6.5997 2.5927 Graphics I I 30- Reject Null Conc-% c c <3£ 000-089-125-1 CETIS™ v1,025B Analyst, Approval:. CETIS Test Summary Page 1 of 2 ReportDate: 05 Jul-06 3:45 PM Link: 09-1379-7450/0605-S065d Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Test No: 14-2485-4300 StartDate: 02 Jun-06 02:40 PM Ending Date: 09 Jun-06 02:10 PM Setup Date: 02 Jun-06 02:40 PM Test Type: Reproduction-Survival (7d) Protocol: EPA/821/R-02-013 (2002) Dil Water: Diluted Mineral Water (8:2) Brine: Nol Applicable Duration: 6d 23h Species: Ceriodaphnia dubia Source: In-House Culture Sample No: 15-4275-6942 Material: Sample Date; 02 Jun-06 09:30 AM Code: ReceiveDate; 02 Jun-06 12:00 PM Source: Sample Age: 5h Station: Groundwater 06-0462 Dudek & Associates Well 3 Client: Project; Dudek & Associates Comparison Summary Analysis Endpoint Coni-rcl i,/. LOndvci^i^My ^*>ntrol^2}l COndi^d-l^i'ty ~ V, fOO NOEL LOEL ChV MSDp Method 12-6666-0163 7d Proportion Survived <0 ^0 05-7604-2943 Reproduction <0 —^0 N/A N/A N/A 11.52% Fisher's Exact Mann-Whitney U Test Acceptability Analysis Endpoint Attribute Statistic Acceptable Range Decision 12-6665-0163 7d Proportion Survived Control Response 1 0.8 - N/A Passes acceptability crite ia • 12-5666-0163 7d Proportion Survived Control Response 0.4 0.8 - N/A ConJ. • CoKf. Relic aeooptobility erfteria 05-7604-2943 Reproduction Control Response 32.7 15 - N/A Passes acceptability crite ia 05-7604-2943 Reproducfion Conlrol Response 4.5 15-N/A Cohd. Co^-^. Folic oGooptability eriterta- 05-7604-2943 Reproduction MSDp 0.11518 0.13-0.47 - foils dLceptdbilily ciiluiia 7d Proportion Survived Summary Conc-% __Control Type Reps Mean 1 Minimum Maximum SE SD CV 0 1 Conductivity C 10 0.40000 1 0-00000 1.00000 0.16330 0.51640 129.10 0 / Lab Control 10 i.oooooj 1.00000 1.00000 0.00000 0.00000 0.00% 6.25 10 1.00000 1-00000 1.00000 0.00000 0.00000 0.00% 12.5 10 1.00000 1.00000 1.00000 0.00000 0.00000 0,00% 25 10 1.00000 1.00000 1.00000 0.00000 0.OOOOO 0.00% 50 10 0.90000 0.00000 1.00000 0.10000 0.31623 35.14% 100 10 0.90000 0.00000 1.00000 0.10000 0.31623 35.14% Reproduction Summary Conc-% ^ontrol Type Reps Mean Minimum Maximum SE SD CV 0 Conductivity C 10 4.5 j 0 14 1.9394 6.1328 136.28 0 Lab Control 10 32.LJ 30 38 0.9781 3.0930 9.46% 6.25 10 31.3 25 35 1.0440 3.3015 10.55% 12.5 10 30.9 27 36 0.9713 3.0714 9.94% 25 10 32.6 30 34 0.4269 1.3499 4.14%) 50 10 26.7 17 33 1.6265 5-1435 19.26% 100 10 8 0 12 1.2472 3.9441 49.30% typical, //puei^ -fke. rt^UhW M-P-Pe^e^cc ^-eft-^ce^ -r^^i^^ Co^V-z-o / t^^f (^r^Ai/c+iu/fy ^rfPe^er^e. Tee EPA docw^^^-t S^^>/jl-oz. 013 (i^^oz) 000-089-125-1 CETIS™ V1.025B Analyst:. SC Approval: CETIS Test Summary Report Date; Link; Page 2 of 2 05 Jul-06 3:45 PM 7d Proportion Survived Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 -, Rep 4 Rep 5 . Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conduclivity C 0.00000 0.00000 1.00000 1-00000 1.00000 1.00000 0.00000 0.00000 0.00000 0.00000 0 Lab Control 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 5.25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 12.5 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 25 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 50 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 100 1.00000 1.00000 0.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Reproduction Detail Conc-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Conductivity C 0 0 10 7 14 14 0 0 0 0 0 Lab Control 32 30 30 31 30 35 34 38 30 37 6.25 34 28 29 35 35 31 33 30 33 25 12.5 27 34 28 32 36 27 32 31 33 29 25 31 34 33 32 34 30 33 34 32 33 50 31 17 25 23 22 26 32 27 33 31 100 8 11 -0 9 12 5 12 8 4 11 000-089-125-1 CETIS™ V1.025B Analyst:. SC Approval CETIS Analysis Detail Comparisons: Report Date: Page 1 of 1 27 Jun-06 1:42 PM Ceriodaphnia 7-d Survival and Reproduction Test Nautilus Environmental (CA) Endpoint Analysis Type Sample Link Control Link Date Analyzed Version 7d Proportion Survived Comparison 09-1379-7450 09-1379-7450 27 Jun-06 1:41PM CETlSvl ,025 Method Alt H Data Transform NOEL LOEL Toxic Units ChV MSDp Fisher s Exact C > T Untransformed <0 0 N/A Group Comparisons Control vs Control Statistic Critical Decision(0.05) Lab Control Conductivity Co 0.00542 0.05000 Significant Effect Data Summary Conc-% Control Type Non-Responders Responders Total Observed 0 Conductivity Co 4 6 10 0 LabControl 10 0 io Graphics 1.0-o 0.9- s > 0,8-W 0,7- c •| 0,6- 1 0,5- ^ 0,4-rs Q 0,3- 0,2- 0,1- 0,0^ 1 1 0 0 (^Lc) Conc-% [ca) 000-089-125-1 CETIS™ v1,025B Analyst: S ^ Approval: Sit l/^/eC CETIS Analysis Detail Ceriodaphnia 7-d Survival and Reproduction Test Comparisons: Page 1 of 1 Report Date: 27 Jun-06 1:42 PM Analysis: 05-7604-2943/0605-S065d Nautilus Environmental (CA) Endpoint Analysis Type Reproduction Comparison Sample Link Control Link Date Analyzed Version 09-1379-7450 09-1379-7450 27 Jun-06 1:41 PM CETISvi:025 Method Alt H Data Transform Mann-Whitney U C >T Untransformed NOEL LOEL Toxic Units ChV <0 0 MSDp N/A 11,52% ANOVA Assumptions Attribute Test Variances Statistic Critical P Level Decision(O.OI) Variance Ratio 3.93147 Distribution Shapiro-Wilk W 6.54109 0,05377 Equal Variances 0,85362 0,86826 0,00526 Non-normal Distribution ANOVA Table Source Between Sum of Squares Mean Square DF F Statistic P Level Decision(0.05) Error 3976,2 424,6 3976,2 23,58889 1 18 168,56 0,00000 Significant Effect Total 4400,79996 3999,7888 19 Group Comparisons Control vs Control Statistic Critical P Level Ties Lab Control Conductivity Co 100 Decision{0.05) 0,0000 Significant Effect Original Data Data Summary Conc-% Control Type Count Mean Minimum Maximum SD Transformed Data Mean Conductivity C 10 4,5 LabControl 10 32,7 Graphics I I 30- 0 30 Minimum Maximum SD 14 38 6,1328 3,0930 cc) Conc-% 5| c 3 ybooo 0 0 o ' 0 Oy< So o -2,0 -1,5 -1,0 -0,5 0,0 0,5 Ranicits 1,0 1,5 2,0 000-089-125-1 CETIS™ v1,025B Analyst: 5c Approval: 7/v7<^6 Freshwater Chronic Bioassay Client/Sample ID: bu^Ack ^ /^So^'O^^ Daphnid Survival and Reproduction Datashe? Test Species: C. dubia D. pulex D. magn, Start Date/Time: M,_2^^Qj^i, End Date/Time: ^1 iTime Fed (day): (0)iHhfi_ (2)J3<to_ (Z)I^l^ (4) \\\< (5) | (6)i^ (7) - (8)_ Notes: L ^ D , 7~ r- -a---^, - u.tiuu (oniy ine first 3 hrnnriQ are Wauti/us Environmenfa/, LLC. 5550 Morehoc.se Drive, Suite 150. San Diego, CA 92121. Final Review: '7/-S7()C I I I Freshwater Chronic Bioassay Client: Sample ID: Test No: Cc Water Quality Measurements Test Species: C d^V;,^ Start Date/Time: C. ^ -pb^fkH^) End Date/Time: i'-'^msG i^jp Analysts: Comments: Concentration Day 0 1 2 3 4 5 6 Initial pH <§.i7 •?.)) ^.\^ *.Vt) DO (mg/L) •7-5 "l.-S 7>.'1 1.^ -I 5 7.5 Cond. (pmtios/cm) •^pU'<;rt> ^fo) D 3(^51 (9 Temp (°C) m ^ Final pH -|.^-2-^,|D i^.Do DO (mg/L) ^. 1 ^-^ mm Temp CC) AM ^3 1 Concentration Day 0 1 2 3 4 5 6 7 - Initial pH %.OD DO (mg/L) -1 Lo Xs-T,(> 7. H Cond. (pmhos/cm) M%CO 'l?>to M7^ -J HT ^0 Temp CC) 5a. •3-M.4 af, 3 ——LJ: > V 1 , , Final PH '7.q'?; S'./O 111? DO (mg/L) 7-4 "^^^ / %.| Temp CC) 2S./ as.m Concentration Day 0 1 2 3 4 5 6 7 - initial pH DO (mg/L) Cond, (pmhos/cm) Tomp (°C) - - Pl"=«1 PH DO (mg/L) Temp (°C) ts: Initial: Final: 0 1 2 3 4 5 6 7 ts: Initial: Final: ts: Initial: Final: Uo 2^ S2i Animal Source/Date Received: QC ChecK: lj>lvifoU • Nauiiliis Environmental. LLC. 5550 Morehouse Drive, Suite 150. San Diego, CA 92121. Animal Age at Initiation: ^I'AKTJ) Final Review: -^fC if-^/oC Appendix B Sample Receipt Information I I Nautilus Environmental 5550 Morehouse Drive, Suite 150 San Diego, CA 92121 Client: Z /ksoQoU^ Sample ID: O^-iUA CLW Pei •7^-/- Xb: Sample (A, B, C) a, Tests Performed: Osr'ioJcipk^'^ Test ID No(s).:_^^OS(~S^5 Check-in Temperature {'C) Temperature OK? DO (mg/L) pH (units) Conductivity (pS/cm) Salinity (ppt) Alkalinity (mg/L)* Hardness (mg/L)* * Total Chlorine (mg/L) Technician Initials • '^)Ur-V,Lf ^ . Vlf.,\\ • ^qL*^ ^ ^ " OMt^ i 'ls\-Cks I1MS 0>A-DU •\Mi'=r Lp.2--OLp oq'^^o (LICKS' \Wb %.\ Y (5) Y § Y (5) ^m 2.1 1.4'=) Q.G 2 2-c^<; ~\\ L^O "71000 O, DX- •jfvc (iia.C Ad/i-nc ! mg/L as CaCO,, '= Measured for freshwater samples only, NA = Not Applicable /Freshwater Tests: / Control/Dilution Wafer Source: (^l) Culligan Other Alkalinity: ^^6 Additional Control? N = Lo^'^'^'mivi ''^'-^ (Wrfrd Alkalinity: / Marine Tests: Hardness: 'S'tf Hardness: ^'d^ Control/Dilution Water Source: U\B SW ART SW Other: Additional Control? Y N = Sample Salted w/ artificial salt? Y N If yes, what ppt? Alkalinity: Alkalinity: Salinity: Salinity: Sample Check-In Information Sample Description: .\fNie\\ \ - WcCsr,^ y^.WcLA^, ' 6 fr,c.C^\xji , - tvc^ 0^.-)r , X^b , COC Complete? Q) . N A B C Filtration? Y (M) Pore Size: Aeration? Organisms or Oebris Final 00: Final pH: pH Adjustment? Y 0). Initial pH: . Final pH: Clj Adjustment? B Initial Free,.C^: ST&added: Finat Free Cl^: Sub'^amples for additional chemistry: Sample salted w/brine? Y N If yes, what ppt? Comments: Temperature for sample must be 0-6°C if received >24 hours past collection time. QG Check: A'^- MinhU Final Review:' s77^. I/XT/O^ Appendix C Chain-of-Custody Forms ° c/ln'f.^^u'^'* ° WASHINGTON • BRITISH COLUMBIA 5550 Moiehouse Dnve • Suife 150 5009 fcdfic Highwoy Eosf • Suite 2 8664 Commerce Court ^0 Dim Mornio 92121 Tocomo, Washington 98424 Burnoby, British Columbia, Canada VSA 4N3 to858.S87.7333 253.922.4296 ^604,420 8773 fox858,587.3961 Fax253,922,5814 Fox604 357 136] Chain of Custody Date Sample Collection by: Report to: Company ^'^^ Address (>0T "^^^ >T City i^ClA-^i W State CAT Zip ^ItV^ Contact ""T^TJ!^ J> h*Y ^ . Phone/Email 76 0 ^"7^ <4i^^ Invoice fo: Company _ Address City —/^ ^. -)4 f State C Zip He^Zy' Contact fr.^,,^ ^Z^aC/^ Phone/Email V/; Q - Y? f^^/ jT'T' ANALYSES REQUIRED PROJEa INFORMATION CLIENT O. NO. SHIPPED VIA; SPECIAL INSTRUaiONS/COMMENTS: TOTAL NO, OF CONTAINERS REC'D GOOD CONDITION /AATCHES TEST SCHEDULE (Prinleci Noni^ (Time (Date) (.Signalurej (Printed Na (Date) (Company) AddiHonal costs may be requirecj for sample disposal or storage. Net 30 unless otiierwise contracted. RELINQUISHED BY (COURIER) (Signature) (Printed Name) (Company) RECEIVED BY (LABOR^ORY}, ^ / / 7r~, M-o,i,A^ ,j,m. (Signature) (Printed Name) (Time) (Date) (Dole). Noutilus Environmental log-in No. DISTRIBUTION: WHITE - NauKlus Environmental, COLOR • Oriainato • CALIFORNIA 5550Morehouse Drive-Suite ISO Son Diego, California 92121 Phone 858.587.7333 Fax 858,587,3961 • WASHINGTON 5009 Pacific Highwoy East. Suite 2 Tocomo, Woshington 98424 Phone 253,922,4296 Fox 253,922,5814 • BRITISH COLUMBIA 8664 Commerce Court Burnoby Brihsh Columbia, Canado VSA 4N3 Pfione 604.420,8773 Fox 604,357,1361 Date Chain of Custody Sample Collection by: Report to: Company U b^^VC Address City. State .Zip. Contact -pQ,\^ sh^DitrA, Phone/Email SAMPLE ID DATE TIME MATRIX Invoice to: Company Address City /^^iL^^ . A f Contact '^/^ x-i Co<rT'L^J //^^ / State _cA_ zip^^ c--Ly ANALYSES REQUIRED Phone/Email • <f'^ f ^ V/> 7 CONTAINER TYPE NUMBER Of CONTAINERS COMMENTS VA;^ 7^ (T) t\/£U_ G/i/5i> PROJEQ INFORMATION CLIENT RO, NO, SHIPPED VIA: SAMPLE RECEIPT TOTAL NO, OF CONTAINERS REC'D GOOD CONDITION MATCHES TEST SCHEDULE 13 SPECIAL INSTRUaiONS/COMMENTS: RELINQUISHED BY (CLIENT) (Printed (Time) (Date) (Company) RECEIVED BY (COURIER) l=;i^or,|i.rol (Printed Name) (Date) (Company) RELINQUISHED BY (COURIER) (Signature) [Printed Name) (Company) RECEIVED BY (LABORATORY) (Signotu.-e) (Printed Name) (Time) (Date) l'2u>£> \ I ime) (Dote) Nautilus Environmental Log-in No. Qjj; • Additional costs may be recjc/ired for sample disposal or storage. Net 30 unless otherwise contracted. DISTRIBUTION; WHITE - Nautilus Environmental, COLOR - Oricjinotor LETTER OF TRANSMITTAL 605 THIRD STREET ' ENCINITAS, CALIFORNIA 92024 T 760.942.5147 F 760.632.8710 To: Dudek 1645 S. Rancho Santa Fe Road Suite 202 San Marcos, California 92078 DATE: July 7, 2006 JOBNO. 4821-01 RE: Carlsbad Municipal Golf Course Water Quality Testing I Attention: George Litzinger We are sending you the following items: JUL 1 0 2006 DUDbK a ASSOCIATES S.AN MftRClgS omn^' Copies DATE NO. DESCRIPTION 3 6/9/2006 Baseline Water Quality Analytical Testing Laboratory Results 3 7/5/2006 Baseline Water Quality Toxicity Testing Laboratory Results THESE ARE SENT as cliecked below: For approval X For your use Other As requested For review and comment Remarks: Enclosed are the first baseline laboratory results for the water quality testing program for the golf course. We shall collect samples for the second baseline sample and submit it to the labs in the near future. Please contact me if you have any questions or wish to discuss. Copy to: John Cahill, City of Carlsbad -Signed: EnviroMatrix I /M\ j Analytical, Inc. 09 June 2006 Dudek & Associates, Inc. EMA Log #: 0606040 Attn: Chris Oesch 605 Third Street Encinitas, CA 92024 Project Name: Carlsbad G.C. Enclosed are the results of analyses for samples received by the laboratory on 06/02/06 11:34. Samples were analyzed pursuant to client request utilizing EPA or other ELAP approved methodologies. I certify that this data is in compliance both technically and for completeness. Dan Verdon Laboratory Director CA ELAP Certification #: 2564 4340 Viewndge Avenue, Suite A • San Diego, California 92123 • (858) 560-7717 • Fax (858) 560-7763 Analytical Chemistry Laboratory Client Name: Dudek & Associates, Inc. ProjectName: Carlsbad G.C. EMA Log #: 0606040 ANALYTICAL REPORT FOR SAMPLES Sample ID Laboratory ID Matrix Date Sampled Date Received Surface Upstream 0606040-01 Water 06/01/06 12:30 06/02/06 11:34 Well 1 0606040-02 Water 06/01/06 14:50 06/02/06 11:34 Surface Downstream 0606040-03 Water 06/01/06 14:00 06/02/06 11:34 Well 3 0606040-04 Water 06/02/06 09:30 06/02/06 11:34 TTie results in this report apply to lhe samples analyzed in accordance wilh the chain of custody document. This analytical report must be reproduced in its entirety. - EnviroMatrix Analytical, Inc. Page 2 of 8 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad G.C. EMA Log #: 0606040 Conventional Chemistry Parameters by Standard/EPA Methods Reporting Analyte Result Limit Units Dilution Batch Prepared Analyzed Method Notes Surface Upstream (0606040-01) Water Sampled: 06/01/06 12:30 Received: 06/02/06 11:34 Nitrate as N 0.39 0.10 mg/l 2 6060218 06/02/06 06/02/06 SM4500 NOS E Nitrite as N ND 0.05 1 6060226 06/02/06 06/02/06 SM4500 N02 B Dissolved Oxygen 3.34 O.IO 6060538 06/02/06 06/02/06 SM4500 0 G pH 7.24 0.10 pH Units 6060506 06/02/06 06/02/06 EPA 150.1 Phosphorus, Total 0.06 0.05 mg/l 6060115 06/02/06 06/02/06 SM4500 P B, E Total Suspended Solids ND 20 6060540 06/05/06 06/05/06 SM2540 D Well 1 (0606040-02) Water Sampled: 06/01/06 14:50 Received: 06/02/06 11:34 Nitrate as N ND 0.05 mg/l 1 6060218 06/02/06 06/02/06 SM4500 N03 E Nitrite asN ND 0.05 II 6060226 06/02/06 06/02/06 SM4500 N02 B Dissolved Oxygen 1.10 0.10 6060538 06/02/06 06/02/06 SM4500 0 G pH 5.90 O.IO pH Units 6060506 06/02/06 06/02/06 EPA 150,1 Phosphorus, Total 0.17 0.05 mg/l 6060115 06/02/06 06/02/06 SM4500 P B, E Total Suspended Solids ND 20 1. 6060540 06/05/06 06/05/06 SM2540D Surface Downstream (0606040-03) Water Sampled: 06/01/06 14:00 Received: 06/02/06 11:34 Nitrate as N ND 0,05 mg/l 1 6060218 06/02/06 06/02/06 SM4500NO3 E Nitrite as N ND 0,05 .1 6060226 06/02/06 06/02/06 SM4500 N02 B Dissolved Oxygen 2.92 0.10 .. 6060538 06/02/06 06/02/06 SM4500 O G pH 7.42 0.10 pH Units 6060506 06/02/06 06/02/06 EPA 150,1 Phosphorus, Total 0.21 0.05 mg/l " 6060115 06/02/06 06/02/06 SM4500 P B, E Total Suspended Solids ND 20 6060540 06/05/06 06/05/06 SM2540 D Well 3 (0606040-04) Water Sampled: 06/02/06 09:30 Received: 06/02/06 11:34 Nitrate as N 9.28 1.25 mg/l 25 6060218 06/02/06 06/02/06 SM4500 N03 E Nitrite as N 0.05 0.05 1 6060226 06/02/06 06/02/06 SM4500 N02 B Dissolved Oxygen 0.94 O.IO II 6060538 06/02/06 06/02/06 SM4500 0 G pH 6.60 0.10 pH Units 6060506 06/02/06 06/02/06 EPA 150.1 Phosphorus, Total 0.36 0.05 mg/l 6060115 06/02/06 06/02/06 SM4500 P B, E Total Suspended Solids 37 20 II 6060540 06/05/06 06/05/06 SM2540 D The results in this report apply lo the samples analyzed in accordance wilh the chain of custody documenl. This analytical report musl be reproduced in its entirety. - EnviroMatrix Analjrtical, Inc. Page 3 of { Client Name: Dudek & Associates, Inc. Project Name: Carlsbad G.C. EMA Lo g#: 0606040 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 6060115 Blank (6060115-BLKl) Prepared & Analyzed: 06/01/06 Phosphoms, Total ND 0.05 mg/l LCS (6060115-BSl) Prepared & Analyzed: 06/01/06 Phosphorus, Total 0.49 0.05 mg/l 0.500 98 80-120 LCS Dup (6060115-BSDl) Prepared & Analyzed: 06/01/06 Phosphorus, Total 0.47 0.05 mg/l 0,500 94 80-120 4 20 Duplicate (6060115-DUPl) Source: 0605502-05 Prepared & Analyzed: 06/01/06 Phosphorus, Total 0.32 0.05 mg/l 0.29 10 20 Matrix Spike (6060115-MSl) Source: 0605502-05 Prepared & Analyzed: 06/01/06 Phosphorus, Total 0.60 0.05 " mg/l 0,500 0,29 62 80-120 QM-05 Matrix Spike Dup (6060115-MSDl) Source: 0605502-05 Prepared & Analyzed: 06/01/06 Phosphorus, Total 0.65 0.05 mg/l 0,500 0,29 72 80-120 8 20 QM-05 Batch 6060218 Blank (6060218-BLKl) Prepared & Analyzed: 06/02/06 Nitrate as N ND 0.05 mg/l LCS (6060218-BSl) Prepared & Analyzed: 06/02/06 Nitrate as N 0.43 0.05 mg/l 0,500 86 80-120 LCS Dup (6060218-BSDl) Prepared & Analyzed: 06/02/06 Nitrate as N 0.46 0.05 mg/l 0.500 92 80-120 7 20 The results in this report apply to the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix I ,ivi I Analytical. Inc. raRc4of8 Client Name: Dudek & Associates, Inc. Project Name: Carlsbad G.C. EMA Log #: 0606040 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 6060218 Duplicate (6060218-DUPl) Source: 0606016-03 Prepared & Analyzed: 06/02/06 Nitrate as N ND 0.05 mg/l ND 20 Matrix Spike (6060218-MSl) Source: 0606016-03 Prepared & Analyzed: 06/02/06 Nitrate as N 0.44 0.05 mg/l 0.500 ND 88 80-120 Matrix Spike Dup (6060218-MSDl) Source: 0606016-03 Prepared & Analyzed: 06/02/06 Nitrate as N 0.40 0.05 mg/l 0.500 ND 80 80-120 10 20 Reference (6060218-SRMl) Prepared & Analyzed: 06/02/06 Nitrate as N 5.69 0.50 mg/l 6.28 91 89-109 Batch 6060226 Blank (6060226-BLKl) Prepared & Analyzed: 06/02/06 Nitrite as N ND 0.05 mg/l LCS (6060226-BSl) Prepared & Analyzed: 06/02/06 Nitrite as N 0.09 0.05 mg/l 0.100 90 80-120 LCS Dup (6060226-BSDl) Prepared & Analyzed: 06/02/06 Nitrite as N 0.09 0.05 mg/l 0,100 90 80-120 0 20 Duplicate (6060226-DUPl) Source: 0606031-07 Prepared & Analyzed: 06/02/06 Nitrite as N ND 0.05 mg/l ND 20 Matrix Spike (6060226-MSl) Source: 0606031-07 Prepared & Analyzed: 06/02/06 Mitrite as N 0.09 0.05 mg/l 0,100 ND 90 80-120 The results in this report apply to the samples analyzed in accordance wilh the chain of custody document. This analytical report must be reproduced in ils entirety. -EnviroMatrix [ I Anal}rtical, Inc. Page 5 of 8 Client Name: Dudek & Associates, Inc. ProjectName: Carlsbad G.C. EMA Log #: 0606040 Conventional Chemistry Parameters by Standard/EPA Methods - Quality Control Analyte Result Reporting Limit Units Spike Source %REC Level Result %REC Limits RPD RPD Limit Notes Batch 6060226 MatrLx Spike Dup (6060226-MSDl) Source: 0606031-07 Prepared & Analyzed: 06/02/06 Nitrite as N 0.09 0.05 mg/l 0.100 ND 90 80-120 0 20 Batch 6060506 Duplicate (6060506-DUPl) Source: 0606037-01 Prepared & Analyzed: 06/02/06 pH 9.56 0.10 pH Units 9.56 0 20 Reference (6060506-SRMl) Prepared & Analyzed: 06/02/06 pH 8.84 0.10 pH Units 9.10 97 97-103 Batch 6060538 Duplicate (6060538-DUPl) Source: 0606040-01 Prepared & Analyzed: 06/02/06 Dissolved Oxygen 3.22 0.10 mg/l 3.34 4 20 Batch 6060540 Duplicate (6060540-DUPl) Source: 0605510-01 Prepared & Analyzed: 06/05/06 Total Suspended Solids 27 20 mg/l 24 12 20 Duplicate (6060540-DUP2) Source: 0605502-05 Prepared & Analyzed: 06/05/06 Total Suspended Solids 6 20 mg/l 8 29 20 QR-04 Reference (6060540-SRMl) Prepared & Analyzed: 06/05/06 Total Suspended Solids 51 20 mg/l 55.7 92 87.3-113 The results in this report apply lo the samples analyzed in accordance with the chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix I jvi^ ] Analytical, Inc. Page 6 of 8 Client Name: Project Name: Dudek & Associates, Inc. Carlsbad G.C. EMA Log #: 0606040 Conventional Chemistry Parameters by Standard/EPA Methods -• Quality Control Analyte Reporting Spike Source Result Limit Units Level Result %REC %REC RPD Limits RPD Limit Notes Batch 6060540 Reference (6060540-SRM2) Total Suspended Solids Prepared & Analyzed: 06/05/06 51 20 mg/l 55.7 92 87,3-113 The results in this report apply io the samples analyzed in accordance with the, chain of custody document. This analytical report must be reproduced in its entirety. EnviroMatrix I M' 1 Analytical, Inc. -Page 7 of 8 Client Name: Dudek & Associates, Inc. EMA Log #: 0606040 Project Name: Carlsbad G.C, Notes and Definitions QR-04 The RPD between the sample and sample duplicate is not valid since both results are below the reporting limit for this analyte. QM-05 The spike recovery was outside acceptance limits for the MS and/or MSD due to matrix interference. The LCS and/or LCSD were within acceptance limits showing that the laboratory is in control and the data is acceptable. ND Analyte NOT DETECTED at or above the reporting limit NR Not Reported dry Sample results reported on a dry weight basis RPD Relative Percent Difference The results in this repori apply to the samples analyzed in accordance with the chain of custody document. This analytical repori must be reproduced in its entirety. -EnviroMatrix ^^^^ Analytical, Inc. Page 8 of 8 CHAIN-OF-CUSTODY RECORD ' E^nviroMatrix EMA LOG #: 0 ()OQO^() Analytical, Inc- 4340 Viewridge Ave., Ste. A • San Diego, CA 92123 • Plione (858) 560-7717 • Fax (858) 560-7763 EMA DATE/TIME STAMP Client: REQUESTED ANALYSIS Address: 6 or 3^ Attn aM(L\l Ofc^CW Phone: ^Hl^bi Sampled by: C-O T^^T Fax: '^ef^'frZ^^'^'^^ Billing Address: Project: C/\(lUS \^ (S.C. P0#: EMA ID n Client Sample ID Sample Date Sample Time Sample Matrix Container(s) # Type* 4 o •2 WM(i>^ 3 5 10 'Container Types: B=Brass Tube; V=VOA; G=Glass; P=Plaslic; 0=Other (list) RELINQUISHED BY Signature DATEATIME RECEIV: Signature Print '^^0^ fct£^f- Tamper-Proof Seals Intact: Yes No N/A Sample(s): Cold Ambient Wann Correct Containers: Yes No VOAsw/ZHS: Yes No N/A AU Samples Properly Preserved: Yes No N/A Disposal: N/C (aqueous) *EMA (@$5,00/sample) Retum Hold^ Turnaround Time: 24 Iir 48 hr 3 day 4 day 5 day ( Normal Commems: c[^t>k ^ Company Company: ^"yy^A^-^jCy Signature Signature Print Print Company: Company: *EMA reserves the righl to return samples that do not match our waste profile. White - EMA Canary - Accounling Pink - Client (w/Repoa) Goldenrod - Client (Kciinquish Samples)